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Strain Name:

B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J

Stock Number:

006147

Availability:

Repository-Cryopreserved

Price and Supply Information

General Terms and Conditions

Genes & Alleles   GFP;   Sfpi1;   Tg(Sfpi1,-EGFP)7Dgt;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Daniel Tenen,   Beth Israel Deaconess Medical Center

Strain Description
Hemizygous mice are viable and fertile with no gross or behavioral abnormalities. Flow cytometry identifies enhanced green fluorescent protein (EGFP) reporter expression in a pattern similar to the endogenous gene; in bone marrow, high expression is observed in myeloid cells, and to a lesser degree in B lymphocytes and erythroid cells. Expression in spleen is consistent with B cells and natural killer cells, while no expression is observed in thymus. These mice may be useful in studies of hematopoietic cell development, transcription factor pathways, and leukemia (including erythroleukemia).

Strain Development
A transgene was created to contain the -14 kb upstream regulatory element (URE), 2.1 kb promoter sequence, and cDNA sequence of the murine PU.1 (Sfpi1 gene) all positioned upstream of an internal ribosomal entry site-enhanced green fluorescent protein (IRES-EGFP, Clonetech) and followed by the rabbit beta-globin gene (containing exon 2-3) and polyadenylation signal. This transgene was injected into FVB/N fertilized oocytes and embryos were implanted into the uteri of pseudopregnant FVB/N foster mothers. Transgenic offspring (founder line 7) were bred to C57BL/6 mice for one generation. The resulting hemizygous mice (mixed C57BL/6;FVB/N background) were intercrossed for many generations prior to arrival at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol Tg(Sfpi1,-EGFP)7Dgt
Allele Name transgene insertion 7, Daniel G Tenen
Strain of OriginFVB/N
Site of ExpressionHigh expression is observed in myeloid cells of bone marrow, and to a lesser degree in B lymphocytes and erythroid cells. Expression in spleen is consistent with B cells and natural killer cells, while no expression is observed in thymus.
Expressed Gene GFP, Green Fluorescent Protein, jellyfish
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source.
Promoter Sfpi1, SFFV proviral integration 1, mouse, laboratory
Molecular Note A Sfpi1 mini-gene comprising a 3.4-kb genomic DNA fragment containing the -14-kb upstream regulatory element (URE), a 2.1-kb genomic segment containing the promoter, and a cDNA representing the processed transcript of the gene is followed by an internal ribosome entry site (IRES), the enhanced green fluorescent protein (EGFP) coding sequence, and exon 2, intron 2, exon 3 and the polyadenylation signal of the rabbit beta-globin gene. Reporter expression is detected in a pattern similar to the endogenous gene, with high expression in myeloid cells, and to a lesser extent in B lymphocytes and erythroid cells. Expression is seen in total bone marrow, but is not detected in the thymus [J:117409]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Tg(Sfpi1,-EGFP)7Dgt

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, transgenic mice are bred together or to wildtype siblings.

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View Fluorescent Protein Strains     (138 strains)

Strains carrying other alleles of GFP
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View Strains carrying other alleles of GFP     (79 strains)

Strains carrying other alleles of Sfpi1
006922   B6.Cg-Sfpi1tm2Dgt/J
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View Strains carrying other alleles of Sfpi1     (3 strains)

Additional Web Information

Fluorescent Proteins/lacZ Systems

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Growth Factors/Receptors/Cytokines
Oncogenes

Developmental Biology Research
Internal/Organ Defects (Lymphoid Tissue Defects)
Internal/Organ Defects (hematopoietic defects)
Lymphoid Tissue Defects (hematopoietic defects)

Hematological Research
Hematopoietic Defects

Immunology and Inflammation Research
Lymphoid Tissue Defects (hematopoietic development)

Research Tools
Cancer Research (Leukemia)
Cancer Research (tumor immunology)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: cell marker for bone marrow transplantation)
Hematological Research
Immunology and Inflammation Research

GFP related

Research Tools
Fluorescent Proteins

References

Selected Reference(s)

Okuno Y; Huang G; Rosenbauer F; Evans EK; Radomska HS; Iwasaki H; Akashi K; Moreau-Gachelin F; Li Y; Zhang P; Gottgens B; Tenen DG. 2005. Potential autoregulation of transcription factor PU.1 by an upstream regulatory element. Mol Cell Biol 25(7):2832-45. [PubMed: 15767686]  [J:117409]

Price and Supply Information

Strain Name: B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
Stock Number: 006147

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        International

Price(s) in US dollars ($)
Cryorecovery Fee $2470.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.

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General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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