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| These R26-stop-EYFP mutant mice have a loxP-flanked STOP sequence followed by the Enhanced Yellow Fluorescent Protein gene (EYFP) inserted into the Gt(ROSA)26Sor locus. When bred to mice expressing Cre recombinase, the STOP sequence is deleted and EYFP expression is observed in the cre-expressing tissue(s) of the double mutant offspring. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points. | |||||||||||||||||||
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 08-SEP-06 Species laboratory mouse Generation N6+F15 (18-JUN-12)
Generation DefinitionsDonating Investigator Frank Costantini, Columbia University Medical Center Description
Mice that are homozygous for the R26-stop-EYFP mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.These R26-stop-EYFP have also been used as a fluorescent reporter by the Allen Institute for Brain Science. For their characterization information, see images at the Allen Institute for Brain Science website (R26-stop-EYFP images).
Mutant mice maintained on a genetic background containing a contribution from C57BL/6J have the potential to harbor a loss-of-function mutation in the nicotinamide (NAD) nucleotide transhydrogenase gene (Nnt, Chromosome 13). This Nnt mutation arose spontaneously in C57BL/6J sometime prior to 1984. It has been confirmed that this strain (Stock No. 006148) is homozygous for the Nnt mutant allele. For a more detailed description, please see the JAX Mice and Services article "Nnt Alleles in C57BL/6 Mice".
Development
A targeting vector was designed to contain the Enhanced Yellow Fluorescent Protein gene (from the pEYFP-N1 plasmid, Clonetech) downstream of a loxP-flanked stop sequence (neomycin resistance gene and a trimer of the SV40 polyadenylation sequence). This entire construct was inserted into the Gt(ROSA)26Sor locus via electroporation of 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in C57BL/6J blastocysts. Chimeric male Y8-8 was bred to a C57BL/6J female mouse. Mutant male progeny were backcrossed to C57BL/6J for 6 generations and then made homozygous prior to sending to The Jackson Laboratory. The Y chromosome may not be fixed to the C57BL/6 genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (358 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(EYFP)Cos | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Frank Costantini | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Gt(ROSA)26Sor-StopFlox-EYFP; LSLYFP; R-EYFP; R26-YFP; R26-stop-EYFP; R26::EYFP; R26R EYFP; R26R-EYFP; R26RYFP; R26REYPF; R26RYFP; R26Y; R26YY; R26eYFP; ROSA-LSL-YFP; ROSA-YFP; ROSA26-EYFP; ROSA26-LoxP-STOP-LoxP-EYFP; ROSA26-YFP; ROSA26-loxP-Stop-loxP-YF; ROSA26-lsl-EYFP; ROSA26-stop-YFP reporter; ROSA26floxSTOPfloxYFP; ROSA26floxSTOP-YFP; ROSA26R-YFP; Rosa-YFPf; Rosa26-YFPfl; Rosa26-eYFPtg; Rosa26YFPStop; Rosa26stopfloxYFP; Rosa26R(YFP); Rosa26YFP; r26YFP; | ||
| Mutation Made By | Frank Costantini, Columbia University Medical Center | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | JM-1 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, Enhanced Yellow Fluorescent Protein expression is observed in the cre-expressing tissues | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Molecular Note | A construct containing a floxed PGK-neo cassette and an enhanced yellow fluorescent protein (EYFP) gene was inserted into the Gt(ROSA)26Sor locus via homologous recombination. The floxed neo cassette contains a strong tpA stop sequence which prevents thetranscription of EYFP. [MGI Ref ID J:80963] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
gT(ROSA)26Sortm1(EYFP)Cos/J MCA SEP,Separated MCA
Gt(Rosa)26Sortm1(EYFP)Cos, Standard PCR
gT(ROSA)26Sortm1(EYFP)Cos/J MCA SEP, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Gt(ROSA)26Sortm1(EYFP)Cos relatedAbou-Khalil R; Le Grand F; Pallafacchina G; Valable S; Authier FJ; Rudnicki MA; Gherardi RK; Germain S; Chretien F; Sotiropoulos A; Lafuste P; Montarras D; Chazaud B. 2009. Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal. Cell Stem Cell 5(3):298-309. [PubMed: 19733541] [MGI Ref ID J:154124]
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Camerer E; Barker A; Duong DN; Ganesan R; Kataoka H; Cornelissen I; Darragh MR; Hussain A; Zheng YW; Srinivasan Y; Brown C; Xu SM; Regard JB; Lin CY; Craik CS; Kirchhofer D; Coughlin SR. 2010. Local protease signaling contributes to neural tube closure in the mouse embryo. Dev Cell 18(1):25-38. [PubMed: 20152175] [MGI Ref ID J:157446]
Camerer E; Regard JB; Cornelissen I; Srinivasan Y; Duong DN; Palmer D; Pham TH; Wong JS; Pappu R; Coughlin SR. 2009. Sphingosine-1-phosphate in the plasma compartment regulates basal and inflammation-induced vascular leak in mice. J Clin Invest 119(7):1871-9. [PubMed: 19603543] [MGI Ref ID J:152547]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 08-SEP-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(EYFP)Cos
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Gt(ROSA)26Sortm1(EYFP)Cos x Homozygous for Gt(ROSA)26Sortm1(EYFP)Cos Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(EYFP)Cos
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Gt(ROSA)26Sortm1(EYFP)Cos x Homozygous for Gt(ROSA)26Sortm1(EYFP)Cos Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
|
|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.