Strain Name: |
B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J |
|---|---|
Stock Number: |
006148 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Gt(ROSA)26Sor; Gt(ROSA)26Sortm1(EYFP)Cos; YFP; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Frank Costantini, Columbia University Generation ?+F4 (23-JUL-07) Strain Description
Mice that are homozygous for the mutant allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice contain an Enhanced Yellow Fluorescent Protein gene (EYFP, Clonetech) inserted into the Gt(ROSA)26Sor locus. Expression of EYFP is blocked by an upstream loxP-flanked STOP sequence. When bred to mice with a cre recombinase gene under the control of a promoter of interest, the STOP sequence of the targeted gene is deleted in the tissue of interest, and EYFP expression is observed. These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.Strain Development
A targeting vector was designed to contain the Enhanced Yellow Fluorescent Protein gene (from the pEYFP-N1 plasmid, Clonetech) downstream of a loxP-flanked stop sequence (neomycin resistance gene and a trimer of the SV40 polyadenylation sequence). This entire construct was inserted into the Gt(ROSA)26Sor locus via electroporation of 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in C57BL/6J blastocysts. Chimeric male Y8-8 was bred to a C57BL/6J female mouse. Mutant male progeny were backcrossed to C57BL/6J for 6 generations and then made homozygous prior to sending to The Jackson Laboratory. The Y chromosome may not be fixed to the C57BL/6 genetic background.
Gene & Allele Details
| Allele Symbol | Gt(ROSA)26Sortm1(EYFP)Cos | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Frank Costantini | ||
| Common Name(s) | R26R-EYFP; R26RYFP; ROSA26-stop-YFP reporter; ROSA26R-YFP; Rosa26-YFPfl; | ||
| Mutation Made By | Frank Costantini, Columbia University | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | JM-1 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | when crossed to a cre recombinase-expressing strain, Enhanced Yellow Fluorescent Protein expression is observed in the cre-expressing tissues | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A construct containing a floxed PGK-neo cassette and an enhanced yellow fluorescent protein (EYFP) gene was inserted into the Gt(ROSA)26Sor locus via homologous recombination. The floxed neo cassette contains a strong tpA stop sequence which prevents thetranscription of EYFP. [MGI Ref ID J:80963] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gt(Rosa)26Sortm1(EYFP)Cos
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (145 strains)
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
YFP relatedResearch Tools
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Developmental Biology Research (Cre-lox System)
Fluorescent Proteins
Research Tools
Fluorescent Proteins
References
Additional ReferencesPrice and Supply Information
| Strain Name: | B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J |
| Stock Number: | 006148 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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