Strain Name: |
NOD.Cg-Tg(Ins2-Cxcl13)1Cys/JbsJ |
|---|---|
Stock Number: |
006154 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Strain Common Names | NOD.BLC; NOD.BLC1; NOD.RIP-BLC; NOD.RIP-BLC1; |
| Genes & Alleles | Cxcl13; Ins2; Tg(Ins2-Cxcl13)1Cys; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain NOD/ShiLt Donor Strain B6D2F1/J Donating Investigator Jeffrey Bluestone, University of California, San Francisco H2 Haplotype g7 Generation N15F?+N1p Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
Ins2-Cxcl13 (commonly referred to as BLC) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. On the B6.D2 background pancreatic islet structure is disrupted, strongly resembling severe insulitis. However, these mice have normal blood glucose levels, no decrease in B-cells and do not become overtly diabetic. Immunohistochemistry indicates Cxcl13 expression is significantly higher in the pancreatic islets than the follicular stromal cells of the lymph nodes while no expression was observed in the liver or kidney. Histopathology indicates 50% of the pancreatic islets are infiltrated with small nucleated cells. Immunohistochemistry and FACS analysis reveals that 90% of the infiltrating cells are B220+ naïve B lymphcytes. In addition, CD4+ and CD8+ cells were present. Transgenic expression leads to the development of lymph node like structures that contain B and T cell zones, high endothelial venules, stromal cells and the chemokine Ccl21 (SLC), Luther SA et al; Immunity 2000.On the NOD congenic background Tg(Ins2-Cxcl13) expression in the pancreas leads to accelerated diabetes (Donating investigator unpublished communication).
This strain is useful to the study of B cells and chemokines in the development of diabetes.
Strain Development
Chemokine Cxcl13 (BLC) is produced by the stromal cells in lymphoid follicles. Lymphoid chemokines have a major role in homeostatic trafficking within lymphoid tissues. This transgenic strain contains the Cxcl13 cDNA fused to rat insulin promoter 7 (Ins2) injected into B6D2F1 fertilized embryos. Approximately, 19 copies of the Ins2-Cxcl13 transgene integrated into the genome. Transgenic mice resulting from B6D2F1 fertilized embyos were mated to C57BL/6 for 10 generations prior to mating to NOD for 15 genrations. In 2006, the T1DR recieved NOD.Cg-Tg(Ins2-Cxcl13)1Cys/JbsJ at generation N15 and crossed it once to NOD/LtJ (Stock# 1976).
Gene & Allele Details
| Allele Symbol | Tg(Ins2-Cxcl13)1Cys | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jason G Cyster | ||
| Common Name(s) | RIP-BLC1; RIP-CXCL13; | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Expressed Gene | Cxcl13, chemokine (C-X-C motif) ligand 13, mouse, laboratory | ||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | Three lines, having 4, 9 and 19 estimated copies of the transgene, were generated. Mice of all three lines exhibited similar B cell-enriched lymphocyte infiltration of the pancreatic beta cells. The line with 19 transgene copies, designated line 1, was used for further analyses. | ||
| Molecular Note | The transgene comprises the full-length Cxcl13 (BLC) cDNA under control of the rat insulin 2 promoter. Immunostaining of adjacent pancreatic sections with antibodies to insulin and to CXCL13 demonstrated that all the beta cells express CXCL3; the intensity of staining for CXCL3 is several fold greater in pancreatic beta cells than in follicular stromal cells, where it is expressed from the endogenous gene. No expression is detected in liver or kidney. [MGI Ref ID J:110548] [MGI Ref ID J:77238] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-Cxcl13)1Cys
Related Strains
Strains carrying other alleles of Cxcl13
005626 B6.129X1-Cxcl13tm1Cys/J View Strains carrying other alleles of Cxcl13 (1 strain)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects (Lymphoid Tissue Defects)
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Lymphoid Tissue Defects (selective lymph node development defects)
References
Selected Reference(s)
Additional ReferencesLuther SA; Lopez T; Bai W; Hanahan D; Cyster JG. 2000. BLC expression in pancreatic islets causes B cell recruitment and lymphotoxin-dependent lymphoid neogenesis. Immunity 12(5):471-81. [PubMed: 10843380] [MGI Ref ID J:110548]
Price and Supply Information
| Strain Name: | NOD.Cg-Tg(Ins2-Cxcl13)1Cys/JbsJ |
| Stock Number: | 006154 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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