Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation ? + F2p   Donating Investigator W. Scott Young III,   National Institute of Mental Health

Description
Mice homozygous for this targeted mutation are viable, fertile, normal in size, exhibit apparently normal sexual behavior, and do not display any gross physical abnormalities. Homozygous mice exhibit less social agression, altered chemoinvestigatory behavior, and impaired social recognition. These mice may be useful in studies of agressive behavior, social motivation, and appropriate behavioral responses, and may be potential models of autism and agression accompanying dementia and traumatic brain injury.

Development
A targeting vector was used to replace the coding region from before the initiating methionine to just upstream of the transmembrane VI region of the endogenous gene with a neomycin resistance cassette. The construct was electroporated into the 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to C57BL/6 to generate mutant colonies on a mixed background.

Control Information

	Control
	Wild-type from the colony
	101043 B6129SF1/J	(approximate)
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Additional Web Information

JAX^® NOTES, Winter 2008; 512. JAX^® Mice strain sheds new light on autism.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Avpr1b^tm1Wsy/Avpr1b^tm1Wsy

        involves: 129S4/SvJae * C57BL/6

• behavior/neurological phenotype
• abnormal social investigation (MGI Ref ID J:94939)
  • no decrease in the amount of time mutant males spend sniffing a female during the second exposure period is seen unlike wild-type males
• decreased aggression (MGI Ref ID J:94939)
  • males display a longer latency to attack and less agonistic behavior towards intruder males
• homeostasis/metabolism phenotype
• increased circulating testosterone level (MGI Ref ID J:94939)
  • testosterone is elevated in young males (60-90 days old) but not in males older than 120 days

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects
Neurodevelopmental Defects (Autism)

Research Tools
Sensorineural Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Avpr1btm1Wsy
Allele Name		targeted mutation 1, WS Young III
Allele Type		Targeted (knock-out)
Common Name(s)		Avpr1b KO; Avpr1b-; V1bR-;
Strain of Origin		129X1/SvJ
ES Cell Line Name		RW-4
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Avpr1b, arginine vasopressin receptor 1B
Chromosome		1
Gene Common Name(s)		AVPR3; V1BR; V3/V1b; V3/V1b pituitary vasopressin receptor; VPR3;
Molecular Note		The coding region from the initiating codon until just before transmembrane region VI was eliminated. [MGI Ref ID J:94939]

Genotyping

Genotyping Information

Genotyping Protocols

Avpr1b^tm1Wsy, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Avpr1b^tm1Wsy related

Caldwell HK; Stewart J; Wiedholz LM; Millstein RA; Iacangelo A; Holmes A; Young WS rd; Wersinger SR. 2006. The acute intoxicating effects of ethanol are not dependent on the vasopressin 1a or 1b receptors. Neuropeptides 40(5):325-37. [PubMed: 17049983]  [MGI Ref ID J:117655]

Lolait SJ; Stewart LQ; Jessop DS; Young WS rd; O'Carroll AM. 2007. The hypothalamic-pituitary-adrenal axis response to stress in mice lacking functional vasopressin V1b receptors. Endocrinology 148(2):849-56. [PubMed: 17122081]  [MGI Ref ID J:129601]

Scattoni ML; McFarlane HG; Zhodzishsky V; Caldwell HK; Young WS; Ricceri L; Crawley JN. 2008. Reduced ultrasonic vocalizations in vasopressin 1b knockout mice. Behav Brain Res 187(2):371-378. [PubMed: 18005969]  [MGI Ref ID J:130127]

Wersinger SR; Ginns EI; O'Carroll AM; Lolait SJ; Young WS rd. 2002. Vasopressin V1b receptor knockout reduces aggressive behavior in male mice. Mol Psychiatry 7(9):975-84. [PubMed: 12399951]  [MGI Ref ID J:94939]

Wersinger SR; Kelliher KR; Zufall F; Lolait SJ; O'Carroll AM; Young WS rd. 2004. Social motivation is reduced in vasopressin 1b receptor null mice despite normal performance in an olfactory discrimination task. Horm Behav 46(5):638-45. [PubMed: 15555506]  [MGI Ref ID J:101895]

Young WS; Li J; Wersinger SR; Palkovits M. 2006. The vasopressin 1b receptor is prominent in the hippocampal area CA2 where it is unaffected by restraint stress or adrenalectomy. Neuroscience 143(4):1031-9. [PubMed: 17027167]  [MGI Ref ID J:117935]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice are viable and fertile with apparently normal sexual behavior, but the donating investigator does not attempt based on the possibility of behavior and parenting problems.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	101043 B6129SF1/J	(approximate)
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.