Strain Name:

B6;129S6-Ptgs1tm1Fun/J

Stock Number:

006182

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Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation?+F1p
Generation Definitions
 
Donating Investigator Colin D. Funk,   Queen's University

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Reduced gene product (protein) levels, 10% of wildtype levels, are detected by Western blot analysis of nonstimulated peritoneal macrophages. RT-PCR analysis of stomach and kidney tissue reveals that gene product (mRNA) levels are reduced by 70-73%. This allele is a hypomorph. Mutant mice have a reduced response to arachidonic acid and capsaicin evoked ear inflammation and to carrageenan induced paw edema. Platelet aggregation is impaired as indicated by resistance to arachidonic acid induced thrombosis in vivo, and failure to aggregate in response to arachidonic acid in vitro. Mutant mice exhibit lengthened bleeding time. This mutant mouse strain may be useful in studies related to inflammatory immune response and thrombosis.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ptgs1
008104   B6.129(FVB)-Ptgs2tm2.1(Ptgs1)Fun/J
View Strains carrying other alleles of Ptgs1     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Preeclampsia/Eclampsia 1; PEE1
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ptgs1tm1Fun/Ptgs1tm1Fun

        involves: 129S6/SvEvTac * C57BL/6
  • immune system phenotype
  • decreased inflammatory response
    • in response to arachidonic acid and capsaicin in an ear swelling assay or carrageenan treatment in a paw edema assay   (MGI Ref ID J:97351)
    • no inflammatory protection was observed with tetradecanoyl phorbol acetate (TPA) and croton oil induced inflammation   (MGI Ref ID J:97351)
  • hematopoietic system phenotype
  • abnormal platelet physiology
    • in ex vivo, platelets did not aggregate in response to concentrations of arachidonic acid that were effective in wild-type platelets   (MGI Ref ID J:97351)
    • mice were resistant to arachidonic acid induced thrombosis in vivo   (MGI Ref ID J:97351)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • normal gestational length, normal parturition, normal litter size and development, normal ovaries size and weight   (MGI Ref ID J:97351)
  • homeostasis/metabolism phenotype
  • abnormal platelet physiology
    • in ex vivo, platelets did not aggregate in response to concentrations of arachidonic acid that were effective in wild-type platelets   (MGI Ref ID J:97351)
    • mice were resistant to arachidonic acid induced thrombosis in vivo   (MGI Ref ID J:97351)
  • abnormal thromboxane level
    • significantly reduced urinary excretion of 1,3-dinor-thromboxane B2   (MGI Ref ID J:108957)
  • decreased urine prostaglandin level
    • significantly reduced urinary excretion of 11alpha-hydroxy-9,15-dioxo-2,3,4,5-tetranor-prostane-1,20 dioic acid   (MGI Ref ID J:108957)
    • significantly reduced urinary excretion of 1,3-dinor-thromboxane B2   (MGI Ref ID J:108957)
  • increased bleeding time
    • tail bleeding time was significantly prolonged   (MGI Ref ID J:97351)
  • renal/urinary system phenotype
  • decreased urine prostaglandin level
    • significantly reduced urinary excretion of 11alpha-hydroxy-9,15-dioxo-2,3,4,5-tetranor-prostane-1,20 dioic acid   (MGI Ref ID J:108957)
    • significantly reduced urinary excretion of 1,3-dinor-thromboxane B2   (MGI Ref ID J:108957)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects
      Thrombosis

Hematological Research
Platelet Defects
      Alterations in platelet aggregation

Immunology, Inflammation and Autoimmunity Research
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ptgs1tm1Fun
Allele Name targeted mutation 1, Colin D Funk
Allele Type Targeted (knock-out)
Common Name(s) COX-1 KD; PGHS1Neo;
Mutation Made By Colin Funk,   Queen's University
Strain of Origin129S6/SvEvTac
ES Cell Line NameTL1/TL-1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Ptgs1, prostaglandin-endoperoxide synthase 1
Chromosome 2
Gene Common Name(s) COX1; COX3; Cox-1; Cox-3; PCOX1; PES-1; PGG/HS; PGHS-1; PGHS1; PHS1; PTGHS; cyclooxygenase 1;
Molecular Note A floxed PGK-neo was inserted into intron 10 to create this hypomorphic allele. Western blot showed that protein was dramatically reduced in mutant macrophages to approximately 10% of wild-type levels. [MGI Ref ID J:97351]

Genotyping

Genotyping Information

Genotyping Protocols

Ptgs1tm1Fun, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Ptgs1tm1Fun related

Cheng Y; Wang M; Yu Y; Lawson J; Funk CD; Fitzgerald GA. 2006. Cyclooxygenases, microsomal prostaglandin E synthase-1, and cardiovascular function. J Clin Invest 116(5):1391-9. [PubMed: 16614756]  [MGI Ref ID J:108957]

Machado FS; Esper L; Dias A; Madan R; Gu Y; Hildeman D; Serhan CN; Karp CL; Aliberti J. 2008. Native and aspirin-triggered lipoxins control innate immunity by inducing proteasomal degradation of TRAF6. J Exp Med 205(5):1077-86. [PubMed: 18411340]  [MGI Ref ID J:136277]

Nemeth K; Leelahavanichkul A; Yuen PS; Mayer B; Parmelee A; Doi K; Robey PG; Leelahavanichkul K; Koller BH; Brown JM; Hu X; Jelinek I; Star RA; Mezey E. 2009. Bone marrow stromal cells attenuate sepsis via prostaglandin E(2)-dependent reprogramming of host macrophages to increase their interleukin-10 production. Nat Med 15(1):42-9. [PubMed: 19098906]  [MGI Ref ID J:146566]

Song WL; Wang M; Ricciotti E; Fries S; Yu Y; Grosser T; Reilly M; Lawson JA; FitzGerald GA. 2008. Tetranor PGDM, an abundant urinary metabolite reflects biosynthesis of prostaglandin D2 in mice and humans. J Biol Chem 283(2):1179-88. [PubMed: 17993463]  [MGI Ref ID J:130179]

Yu Y; Cheng Y; Fan J; Chen XS; Klein-Szanto A; Fitzgerald GA; Funk CD. 2005. Differential impact of prostaglandin H synthase 1 knockdown on platelets and parturition. J Clin Invest 115(4):986-995. [PubMed: 15776109]  [MGI Ref ID J:97351]

Yu Y; Fan J; Hui Y; Rouzer CA; Marnett LJ; Klein-Szanto AJ; FitzGerald GA; Funk CD. 2007. Targeted cyclooxygenase gene (ptgs) exchange reveals discriminant isoform functionality. J Biol Chem 282(2):1498-506. [PubMed: 17110378]  [MGI Ref ID J:118171]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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