Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N6+F1pN1 Generation Definitions   Donating Investigator Richard J Hodes,   NCI/NIH

Description
Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of activated spleen lymphocytes. While neither telomere shortening nor chromosomal abnormalities (even across multiple generations) are observed, homozygous mice have significantly decreased body weight. These mutant mice may be useful in studies of both telomerase function and telomerase-independent pathways which affect development and metabolism.

Development
A targeting vector was designed to place a loxP-flanked neomycin resistance cassette immediately upstream of exon 1 of the endogenous gene, as well as insert a third loxP site downstream of exon 1. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Mice carrying the "floxed" allele were then bred together to produce homozygotes (TANK2^flox/flox) and next bred to C57BL/6 mice carrying the EIIa-Cre transgene to remove exon 1 (leaving a single loxP site). Mice bearing this "TANK2 exon 1-deleted" allele were backcrossed to C57BL/6J for six generations (and selected againt the EIIa-Cre transgene) prior to arrival at The Jackson Laboratory.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tnks2^tm1.1Yjc/Tnks2^tm1.1Yjc

        involves: 129S1/Sv

• mortality/aging
• *normal* mortality/aging
  • homozygotes give no indication of embryonic lethality   (MGI Ref ID J:106918)
• adipose tissue phenotype
• abnormal adipocyte glucose uptake
  • adipose glucose uptake is decreased in response to insulin stimulation compared to in wild-type mice   (MGI Ref ID J:137985)
• growth/size phenotype
• decreased body weight   (MGI Ref ID J:137985)
  • lower weight in females seen primarily between weeks 5 and 13, weights at 42 weeks not significantly different from controls   (MGI Ref ID J:106918)
  • male weights 20% less than controls throughout 42 week observation period   (MGI Ref ID J:106918)
• cellular phenotype
• *normal* cellular phenotype
  • no alteration in telomere length detected over the course of 7 generations   (MGI Ref ID J:106918)
  • mice exhibit normal cell cycling, irradiation sensitivity and telomere length   (MGI Ref ID J:137985)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators

Cancer Research
Genes Regulating Growth and Proliferation
Other
      DNA Repair

Cell Biology Research
Cell Cycle Regulation
DNA Damage Response
Genes Regulating Growth and Proliferation
Post-translational Processing
Transcriptional Regulation

Developmental Biology Research

Diabetes and Obesity Research
Obesity Without Diabetes

Endocrine Deficiency Research
Adipose Defects

Internal/Organ Research
Adipose Defects

Research Tools
Apoptosis Research
Cancer Research
Cell Biology Research
Developmental Biology Research
Diabetes and Obesity Research
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tnks2tm1.1Yjc
Allele Name		targeted mutation 1.1, Y Jeffrey Chiang
Allele Type		Targeted (knock-out)
Common Name(s)		Tank2-;
Mutation Made By		Jeffrey Chiang,   NCI/NIH
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		CJ7
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Tnks2, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
Chromosome		19
Gene Common Name(s)		5430432P15Rik; AA517131; AI662480; ARTD6; PARP-5b; PARP-5c; PARP5B; PARP5C; RIKEN cDNA 5430432P15 gene; TANK2; TNKL; expressed sequence AA517131; expressed sequence AI662480; pART6;
Molecular Note		Exon 1 was removed via cre-mediated excision. RT-PCR failed to detect transcript in mutants. [MGI Ref ID J:106918]

Genotyping

Genotyping Information

Genotyping Protocols

Tnks2^tm1.1Yjc, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chiang YJ; Nguyen ML; Gurunathan S; Kaminker P; Tessarollo L; Campisi J; Hodes RJ. 2006. Generation and characterization of telomere length maintenance in tankyrase 2-deficient mice. Mol Cell Biol 26(6):2037-43. [PubMed: 16507984]  [MGI Ref ID J:106918]

Additional References

Tnks2^tm1.1Yjc related

Chiang YJ; Hsiao SJ; Yver D; Cushman SW; Tessarollo L; Smith S; Hodes RJ. 2008. Tankyrase 1 and tankyrase 2 are essential but redundant for mouse embryonic development. PLoS ONE 3(7):e2639. [PubMed: 18612384]  [MGI Ref ID J:137985]

Levaot N; Voytyuk O; Dimitriou I; Sircoulomb F; Chandrakumar A; Deckert M; Krzyzanowski PM; Scotter A; Gu S; Janmohamed S; Cong F; Simoncic PD; Ueki Y; La Rose J; Rottapel R. 2011. Loss of Tankyrase-Mediated Destruction of 3BP2 Is the Underlying Pathogenic Mechanism of Cherubism. Cell 147(6):1324-39. [PubMed: 22153076]  [MGI Ref ID J:178917]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, heterozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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