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Strain Name:

FVB.129S6-Gt(ROSA)26Sortm1(HIF1A/luc)Kael/J

Stock Number:

006206

Availability:

Repository- Live


General Terms and Conditions

Genes & Alleles   Gt(ROSA)26Sor;   Gt(ROSA)26Sortm1(HIF1A/luc)Kael;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator William Kaelin,   Dana Farber Cancer Institute
GenerationN7+N2 (03-DEC-07)

Strain Description
Mice heterozygous for this "ODD-luc" knock-in are viable and fertile with no gross phenotypic or behavioral abnormalities. These mice have the C-terminal portion of the hypoxia-inducible factor 1 alpha (HIF1A) oxygen-dependent degradation domain (ODD) fused to the firefly luciferase (luc) gene. This region of the ODD also contains a proline residue (amino acid 564) that, when hydroxylated, will serve as a binding site for von Hippel-Lindau tumor suppressor protein (pVHL). Under normal oxygen concentrations, prolyl hydroxylation by egg-laying-defective nine (EGLN) proteins leads to pVHL-dependent polyubiquitylation and proteasomal degradation (thus, little or no luciferase fluorescence). Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization of the fusion protein and high levels of luciferase fluorescence in the hypoxic tissue(s). These "ODD-Luc" bioluminescent reporter mice may be useful in researching transcriptional regulation of hypoxia-inducible genes, cancer, ischemia, cardiovascular, myocardial infarction, stroke, pharmacokinetics, or other studies where imaging/reporting the development of tissue hypoxia and the action of small molecule inhibitors of HIF prolyl hydroxylase activity are appropriate.

Strain Development
A fusion protein was created with a human hypoxia-inducible factor 1 alpha sequence coding for amino acids 530-652 (HIF1a [aa 530-652]; this includes the C-terminal portion of the oxygen-dependent degradation domain [ODD]) fused to the firefly luciferase (luc) gene. This “ODD-Luc” fusion protein was preceded by a loxP-flanked PGK-neo cassette and followed by a bovine polyadenylation stop sequence. This targeting construct was incorporated into the Gt(ROSA)26Sor locus via electroporation into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into C57BL/6 blastocysts. Chimeric mice were bred to EIIA-Cre transgenic mice (on the FVB genetic background) to remove a loxP-flanked neomycin cassette. The resulting mice (with a single loxP site ahead of the “ODD-Luc” fusion gene) were bred with wildtype FVB mice (and selected against the Cre transgene) for 6 generations prior to arrival at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Gt(ROSA)26Sortm1(HIF1A/luc)Kael/Gt(ROSA)26Sor+

        involves: 129S6/SvEvTac
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:118308)
    • fusion gene is expressed ubiquitously; with tissue hypoxia, luciferase expression is enhanced in those tissues

Gene & Allele Details

Allele Symbol Gt(ROSA)26Sortm1(HIF1A/luc)Kael
Allele Name targeted mutation 1, William G Kaelin
Common Name(s) ODD-Luc; ROSA26 ODD-Luc;
Mutation Made By William Kaelin,   Dana Farber Cancer Institute
Strain of Origin129S6/SvEvTac
ES Cell Line NameTC-1
ES Cell Line Strain129S6/SvEvTac
Site of ExpressionUnder normal oxygen concentrations the (HIF1A) oxygen-dependent degradation domain(ODD)/luciferase fusion protein is degraded and shows little or no luciferase fluorescence. Under hypoxia, ubiquitination is attenuated, resulting in stabilization of the fusion protein and high levels of luciferase fluorescence in the hypoxic tissue(s).
Gene Symbol and Name Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Chromosome 6
Gene Common Name(s) AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26;
Molecular Note The human hypoxia-inducible factor 1 alpha sequence coding for amino acids 530-652, including the C-terminal portion of the oxygen-dependent degradation domain (ODD) was fused to the firefly luciferase (luc). The fusion protein was preceded by a loxP-flanked PGK-neo cassette and followed by a bovine polyadenylation sequence. The loxP-flanked neomycin was removed by crossing to a cre-expressing mouse line. In the resulting mice, hypoxia results in stabilization of the fusion protein and enhanced fluorescence detectable in hypoxic tissue. [MGI Ref ID J:118308]

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Gt(ROSA)26Sortm1(HIF1A/luc)Kael

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice may be bred as heterozygotes or homozygotes.
Diet Information LabDiet® 5K52/5K67

Related Strains

Fluorescent Protein Strains
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
005483   129-Tg(CAG-EYFP)7AC5Nagy/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
006102   B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
006100   B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J
005999   B6(SJL)-Tg(SBE/Tk-luc)7Twc/J
007676   B6.129(CD1)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
004218   B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
006412   B6.129-Il12btm1Lky/J
008451   B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ
005582   B6.129P-Cx3cr1tm1Litt/J
007572   B6.129P2(Cg)-Rorctm2Litt/J
005693   B6.129P2-Cxcr6tm1Litt/J
007669   B6.129S4-Pdgfratm11(EGFP)Sor/J
006852   B6.129S6-Per2tm1Jt/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
003479   B6.C3-Tg(Fos-luc)1Rnd/J
006772   B6.Cg-Foxp3tm2Tch/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
005491   B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
007484   B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
006055   B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006051   B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J
005884   B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
005029   B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
006098   B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J
006864   B6.Cg-Tg(Ins1-EGFP)1Hara/J
005244   B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
007902   B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J
007894   B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
004659   B6.Cg-Tg(TIE2GFP)287Sato/1J
006101   B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
003710   B6.Cg-Tg(Thy1-CFP)23Jrs/J
007940   B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007612   B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615   B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
005630   B6.Cg-Tg(Thy1-EYFP)15Jrs/J
003709   B6.Cg-Tg(Thy1-YFP)16Jrs/J
005627   B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
003782   B6.Cg-Tg(Thy1-YFPH)2Jrs/J
007606   B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
004509   B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006417   B6.FVB-Tg(Npy-hrGFP)1Lowl/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
008295   B6;129-Syt9tm1Sud/J
006676   B6;129P2-Olfr151tm26Mom/MomJ
006667   B6;129P2-Omptm3Mom/MomJ
004858   B6;129S1-Tshrtm1Rmar/J
007843   B6;129S4-Efnb2tm2Sor/J
008214   B6;129S4-Pou5f1tm2Jae/J
006614   B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J
006617   B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
004654   B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
005621   B6;D2-Tg(S100B-EGFP)1Wjt/J
005620   B6;D2-Tg(S100B-EYFP)1Wjt/J
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
007610   B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
004190   C.129-Il4tm1Lky/J
005700   C.129P2-Cxcr6tm1Litt/J
006769   C.Cg-Foxp3tm2Tch/J
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
007860   C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
003718   FVB-Tg(GadGFP)45704Swn/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
003257   FVB/N-Tg(GFAPGFP)14Mes/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
006741   STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)BMom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
006570   STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
007879   STOCK Stx1atm2Sud/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
006784   STOCK Tg(Ins1-ECFP)24Hara/J
003658   STOCK Tg(TIE2GFP)287Sato/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
003274   STOCK Tg(tetNZL)2Bjd/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Fluorescent Protein Strains     (145 strains)

Strains carrying other alleles of Gt(ROSA)26Sor
002292   129-Gt(ROSA)26Sor/J
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
003310   129S-Gt(ROSA)26Sortm1Sor/J
003946   129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
007689   129S4/SvJaeSor-Gt(ROSA)26Sortm4(attB/attP)Sor/J
007708   B6.129-Gt(ROSA)26Sortm1(HD*103Q)Xwy/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
003474   B6.129S4-Gt(ROSA)26Sortm1Sor/J
002192   B6.129S7-Gt(ROSA)26Sor/J
006148   B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
003504   B6;129-Gt(ROSA)26Sortm1Sho/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
002073   B6;129S-Gt(ROSA)26Sor/J
003309   B6;129S4-Gt(ROSA)26Sortm1Sor/J
004598   B6;129S4-Gt(ROSA)26Sortm2Dym/J
007670   B6;129S4-Gt(ROSA)26Sortm3(phiC31*)Sor/J
002955   C.129S7-Gt(ROSA)26Sor/J
007900   C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
005420   C;129S7 Gt(ROSA)26Sor-Bmp5cfe-se7J/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
008159   STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
007577   STOCK Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
View Strains carrying other alleles of Gt(ROSA)26Sor     (36 strains)

Additional Web Information

Congenic Nomenclature
Fluorescent Proteins/lacZ Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Ischemia studies

Internal/Organ Research
Heart Abnormalities
Lung Defects
Wound Healing

Research Tools
Cancer Research
Cardiovascular Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Internal/Organ Research
Metabolism Research
Toxicology Research

References

Selected Reference(s)

Safran M; Kim WY; O'Connell F; Flippin L; Gunzler V; Horner JW; Depinho RA; Kaelin WG Jr. 2006. Mouse model for noninvasive imaging of HIF prolyl hydroxylase activity: assessment of an oral agent that stimulates erythropoietin production. Proc Natl Acad Sci U S A 103(1):105-10. [PubMed: 16373502]  [MGI Ref ID J:118308]


Price and Supply Information

Strain Name: FVB.129S6-Gt(ROSA)26Sortm1(HIF1A/luc)Kael/J
Stock Number: 006206

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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