Strain Name:

B6;129S6-Pdzk1tm1Dls/J

Stock Number:

006208

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation?+F1pN1
Generation Definitions
 
Donating InvestigatorDr. David L. Silver,   Albert Einstein College of Medicine

Description
Mice homozygous for this targeted mutation are viable and fertile. Liver tissue from homozygous mutant mice lacks endogenous protein expression. Homozygotes, but not heterozygotes, have significantly decreased (~85-95%) hepatic high density lipoprotein (HDL) receptor scavenger receptor B-I (SR-BI) levels. This decrease is further exacerbated following diet supplementation with the PPAR-alpha activator fenofibrate. These mice may be useful in studies of cardiovascular health and atherosclerosis, lipid metabolism, SR-B1 regulation, kidney function, as well as kidney transporter (e.g. urate transporter) regulation and liver organic anion transport.

Development
A targeting vector was designed to replace exon 1 and part of intron 1 of the endogenous gene with a neomycin resistance cassette. This construct was electroporated into 129S6/SvEvTac-derived CSL3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric mice were bred with C57BL/6J to generate heterozygous mice on this mixed B6;129S6 genetic background. Heterozygotes were then bred together for many generations prior to arrival at The Jackson Laboratory.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Pdzk1tm1Dls/Pdzk1tm1Dls

        involves: 129S6/SvEvTac * C57BL/6J
  • homeostasis/metabolism phenotype
  • abnormal circulating protein level
    • fractional uptake rate of BSP is reduced by 25% in mutants compared with wild-type and plasma half life is increased correspondingly be 25% in knockouts   (MGI Ref ID J:107125)
  • liver/biliary system phenotype
  • abnormal liver morphology
    • scavenger receptor B, type I expression and function in mutant livers are markedly reduced in knockouts   (MGI Ref ID J:107125)
    • distribution of organic anion transporting protein 1A1 is predominantly in intracellular structures, while in wild-type it has a basolateral distribution   (MGI Ref ID J:107125)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Atherosclerosis
Other
      altered lipoprotein profile

Cell Biology Research
Channel and Transporter Defects

Internal/Organ Research
Kidney Defects
Liver Defects

Metabolism Research
Lipid Metabolism

Research Tools
Cardiovascular Research
Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Pdzk1tm1Dls
Allele Name targeted mutation 1, David L Silver
Allele Type Targeted (knock-out)
Common Name(s) PDZK1-;
Mutation Made ByDr. David Silver,   Albert Einstein College of Medicine
Strain of Origin129S6/SvEvTac
ES Cell Line NameCSL3
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Pdzk1, PDZ domain containing 1
Chromosome 3
Gene Common Name(s) 1700023D20Rik; 2610507N21Rik; 4921513F16Rik; AI267131; AI314638; AL022680; CAP70; CLAMP; D3Ertd537e; DNA segment, Chr 3, ERATO Doi 537, expressed; NHERF-3; NHERF3; PDZD1; RIKEN cDNA 1700023D20 gene; RIKEN cDNA 2610507N21 gene; RIKEN cDNA 4921513F16 gene; expressed sequence AI267131; expressed sequence AI314638; expressed sequence AL022680; mPDZK1;
Molecular Note Exon 1, containing the ATG, and part of intron 1 were replaced by neomycin resistance cassette. Western blot of mutant liver lysates confirmed absence of protein. [MGI Ref ID J:100280]

Genotyping

Genotyping Information

Genotyping Protocols

Pdzk1tm1Dls, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lan D; Silver DL. 2005. Fenofibrate induces a novel degradation pathway for scavenger receptor B-I independent of PDZK1. J Biol Chem 280(24):23390-6. [PubMed: 15837786]  [MGI Ref ID J:100280]

Additional References

Pdzk1tm1Dls related

Giral H; Cranston D; Lanzano L; Caldas Y; Sutherland E; Rachelson J; Dobrinskikh E; Weinman EJ; Doctor RB; Gratton E; Levi M. 2012. NHE3 regulatory factor 1 (NHERF1) modulates intestinal sodium-dependent phosphate transporter (NaPi-2b) expression in apical microvilli. J Biol Chem 287(42):35047-56. [PubMed: 22904329]  [MGI Ref ID J:191894]

Wang P; Wang JJ; Xiao Y; Murray JW; Novikoff PM; Angeletti RH; Orr GA; Lan D; Silver DL; Wolkoff AW. 2005. Interaction with PDZK1 is required for expression of organic anion transporting protein 1A1 on the hepatocyte surface. J Biol Chem 280(34):30143-9. [PubMed: 15994332]  [MGI Ref ID J:107125]

Zhou H; Li Z; Silver DL; Jiang XC. 2006. Cholesteryl ester transfer protein (CETP) expression enhances HDL cholesteryl ester liver delivery, which is independent of scavenger receptor BI, LDL receptor related protein and possibly LDL receptor. Biochim Biophys Acta 1761(12):1482-8. [PubMed: 17055779]  [MGI Ref ID J:118151]

Zhu W; Saddar S; Seetharam D; Chambliss KL; Longoria C; Silver DL; Yuhanna IS; Shaul PW; Mineo C. 2008. The scavenger receptor class B type I adaptor protein PDZK1 maintains endothelial monolayer integrity. Circ Res 102(4):480-7. [PubMed: 18174467]  [MGI Ref ID J:147660]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101043 B6129SF1/J (approximate)
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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