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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N?+1F4 (10-DEC-07) Donating Investigator Dr. David Silver, Albert Einstein College of Medicine Description
Mice homozygous for this targeted mutation are viable and fertile. Liver tissue from homozygous mutant mice lacks endogenous protein expression. Homozygotes, but not heterozygotes, have significantly decreased (~85-95%) hepatic high density lipoprotein (HDL) receptor scavenger receptor B-I (SR-BI) levels. This decrease is further exacerbated following diet supplementation with the PPAR-alpha activator fenofibrate. These mice may be useful in studies of cardiovascular health and atherosclerosis, lipid metabolism, SR-B1 regulation, kidney function, as well as kidney transporter (e.g. urate transporter) regulation and liver organic anion transport.Development
A targeting vector was designed to replace exon 1 and part of intron 1 of the endogenous gene with a neomycin resistance cassette. This construct was electroporated into 129S6/SvEvTac-derived CSL3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric mice were bred with C57BL/6J to generate heterozygous mice on this mixed B6;129S6 genetic background. Heterozygotes were then bred together for many generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
Pdzk1tm1Dls/Pdzk1tm1Dls
involves: 129S6/SvEvTac * C57BL/6J
- homeostasis/metabolism phenotype
- abnormal circulating protein level (MGI Ref ID J:107125)
- fractional uptake rate of BSP is reduced by 25% in mutants compared with wild-type and plasma half life is increased correspondingly be 25% in knockouts
- liver/biliary system phenotype
- abnormal liver morphology (MGI Ref ID J:107125)
- scavenger receptor B, type I expression and function in mutant livers are markedly reduced in knockouts
- distribution of organic anion transporting protein 1A1 is predominantly in intracellular structures, while in wild-type it has a basolateral distribution
This mouse can be used to support research in many areas including:
Cardiovascular Research
Atherosclerosis
Other (altered lipoprotein profile)
Cell Biology Research
Channel and Transporter Defects
Internal/Organ Research
Kidney Defects
Liver Defects
Metabolism Research
Lipid Metabolism
Research Tools
Cardiovascular Research
Metabolism Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Pdzk1tm1Dls | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, David L Silver | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | PDZK1-; | ||
| Mutation Made By | Dr. David Silver, Albert Einstein College of Medicine | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | iTL1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Pdzk1, PDZ domain containing 1 | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | 1700023D20Rik; 2610507N21Rik; 4921513F16Rik; AI267131; AI314638; AL022680; CAP70; CLAMP; D3Ertd537e; DNA segment, Chr 3, ERATO Doi 537, expressed; NHERF3; PDZD1; RIKEN cDNA 1700023D20 gene; RIKEN cDNA 2610507N21 gene; RIKEN cDNA 4921513F16 gene; expressed sequence AI267131; expressed sequence AI314638; expressed sequence AL022680; mPDZK1; | ||
| Molecular Note | Exon 1, containing the ATG, and part of intron 1 were replaced by neomycin resistance cassette. Western blot of mutant liver lysates confirmed absence of protein. [MGI Ref ID J:100280] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Pdzk1tm1Dls, SEP PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Lan D; Silver DL. 2005. Fenofibrate induces a novel degradation pathway for scavenger receptor B-I independent of PDZK1. J Biol Chem 280(24):23390-6. [PubMed: 15837786] [MGI Ref ID J:100280]
Additional References
Pdzk1tm1Dls relatedWang P; Wang JJ; Xiao Y; Murray JW; Novikoff PM; Angeletti RH; Orr GA; Lan D; Silver DL; Wolkoff AW. 2005. Interaction with PDZK1 is required for expression of organic anion transporting protein 1A1 on the hepatocyte surface. J Biol Chem 280(34):30143-9. [PubMed: 15994332] [MGI Ref ID J:107125]
Zhou H; Li Z; Silver DL; Jiang XC. 2006. Cholesteryl ester transfer protein (CETP) expression enhances HDL cholesteryl ester liver delivery, which is independent of scavenger receptor BI, LDL receptor related protein and possibly LDL receptor. Biochim Biophys Acta 1761(12):1482-8. [PubMed: 17055779] [MGI Ref ID J:118151]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Pdzk1tm1Dls *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Pdzk1tm1Dls x Homozygous for Pdzk1tm1Dls
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Pdzk1tm1Dls *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Pdzk1tm1Dls x Homozygous for Pdzk1tm1Dls
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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