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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7+N1F2pN1
Generation DefinitionsDonating Investigator Daniel G. Tenen, Beth Israel Deaconess Medical Center Description
Hemizygotes are viable, fertile, normal in size, and do not display any behavioral abnormalities. Hemizygotes express tetracycline-controlled transactivator protein (tTA) in bone marrow hematopoietic stem cells (HSC) and in common myeloid progenitors (CMP)). tTA activity also is detected in lung. Little to no tTA activity is detected in thymus, lymph node, intestine or spleen. When bred to other transgenic mice carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring can be induced by withdrawal of tetracycline or doxycycline. This strain represents an effective tool for generating bitrangenic animals to study inducible gene expression in blood stem and progenitor cells.These mice were originally designed to be bred with transgenic mice harboring a TRE-BCR-ABL1 transgene (Stock No. 006202), creating bitransgenic offspring as a model for studies of the Philadelphia chromosome and inducible chronic myeloid leukemia.
Development
A transgenic construct consisting of the SV40 minimal promoter, rabbit beta-globin intron 2, and the 3' enhancer sequence from the murine T-cell acute lymphocytic leukemia 1 gene (Tal1, formerly called homolog of human stem cell leukemia gene or SCL) was designed to direct expression of the tetracycline-regulated transactivator, tTA. The construct was injected into the pronucleus of "BDF" (C57BL/6 x DBA/2 F1 hybrid) eggs, which subsequently were implanted into pseudopregnant foster mothers. Transgenic mice were bred to generate founder line 19. In 2004, mice were bred to a "tetO-cre" transgenic mouse on an unidentified background. Subsequently, mice were selected for the Tal1-tTA transgene (and against tetO-cre) while backcrossing to FVB/N for approximately 7-9 generations prior to arrival at the Jackson Laboratory. The donating investigators note that tissues from these mice can transplant into FVB/N mice without rejection.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Tal1-tTA)19Dgt allele
017722 B6.Cg-Tg(Tal1-tTA)19Dgt/J View Strains carrying Tg(Tal1-tTA)19Dgt (1 strain)
002319 B6.Cg-Tg(BCL2)22Wehi/J 002320 B6.Cg-Tg(BCL2)25Wehi/J 002321 B6.Cg-Tg(BCL2)36Wehi/J 010914 B6.Cg-Tg(Emu-TXLNA)1Amjr/J 002318 C.Cg-Tg(BCL2)22Wehi/J 003477 C57BL/6J-Tg(SV)419Bri/J 003476 C57BL/6J-Tg(SV)427Bri/J 002233 C57BL/6J-Tg(SV)7Bri/J 003268 FVB-Tg(IRS1)1Mhep/J 006553 STOCK Smn1tm1Msd Tg(H2-K1-tsA58)6Kio Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cancer Research
Chronic Myelogenous Leukemia
Increased Tumor Incidence
Leukemia
Leukemia: lymphocytic
Developmental Biology Research
Internal/Organ Defects
hematopoietic defects
Lymphoid Tissue Defects
hematopoietic defects
Hematological Research
Hematopoietic Defects
Immunology, Inflammation and Autoimmunity Research
Lymphoid Tissue Defects
Research Tools
Cancer Research
Leukemia
Tetop Tet System
myeloma and hybridoma production
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Hematological Research
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Tal1-tTA)19Dgt | ||
|---|---|---|---|
| Allele Name | transgene insertion 19, Daniel G Tenen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | SCLtTA; SCLtTA stg; Scl-tTA; | ||
| Mutation Made By | Daniel Tenen, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Site of Expression | Expresses tTA in bone marrow-derived hematopoietic stem cells (HSC) and common myeloid progenitors (CMP). tTA activity also is detected in lung. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | SV40, E mu enhancer, SV40 | ||
| Molecular Note | The transgene consists of the SV40 minimal promoter, rabbit beta-globin intron 2, and the 3' enhancer sequence from the mouse T-cell acute lymphocytic leukemia gene driving expression of the tetracycline-regulated transactivator. [MGI Ref ID J:96511] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Tal1-tTA)19Dgt, Separated PCR
Tg(tTA), Melt Curve Analysis
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Koschmieder S; Gottgens B; Zhang P; Iwasaki-Arai J; Akashi K; Kutok JL; Dayaram T; Geary K; Green AR; Tenen DG; Huettner CS. 2005. Inducible chronic phase of myeloid leukemia with expansion of hematopoietic stem cells in a transgenic model of BCR-ABL leukemogenesis. Blood 105(1):324-34. [PubMed: 15331442] [MGI Ref ID J:96511]
Additional References
Tg(Tal1-tTA)19Dgt relatedChen CI; Koschmieder S; Kerstiens L; Schemionek M; Altvater B; Pscherer S; Gerss J; Maecker HT; Berdel WE; Juergens H; Lee PP; Rossig C. 2012. NK cells are dysfunctional in human chronic myelogenous leukemia before and on imatinib treatment and in BCR-ABL-positive mice. Leukemia 26(3):465-74. [PubMed: 21904381] [MGI Ref ID J:181529]
Eiring AM; Harb JG; Neviani P; Garton C; Oaks JJ; Spizzo R; Liu S; Schwind S; Santhanam R; Hickey CJ; Becker H; Chandler JC; Andino R; Cortes J; Hokland P; Huettner CS; Bhatia R; Roy DC; Liebhaber SA; Caligiuri MA; Marcucci G; Garzon R; Croce CM; Calin GA; Perrotti D. 2010. miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts. Cell 140(5):652-65. [PubMed: 20211135] [MGI Ref ID J:160777]
Hamilton A; Helgason GV; Schemionek M; Zhang B; Myssina S; Allan EK; Nicolini FE; Muller-Tidow C; Bhatia R; Brunton VG; Koschmieder S; Holyoake TL. 2012. Chronic myeloid leukemia stem cells are not dependent on Bcr-Abl kinase activity for their survival. Blood 119(6):1501-10. [PubMed: 22184410] [MGI Ref ID J:181724]
Moore CR; Liu Y; Shao C; Covey LR; Morse HC 3rd; Xie P. 2012. Specific deletion of TRAF3 in B lymphocytes leads to B-lymphoma development in mice. Leukemia 26(5):1122-7. [PubMed: 22033491] [MGI Ref ID J:188983]
Reynaud D; Pietras E; Barry-Holson K; Mir A; Binnewies M; Jeanne M; Sala-Torra O; Radich JP; Passegue E. 2011. IL-6 controls leukemic multipotent progenitor cell fate and contributes to chronic myelogenous leukemia development. Cancer Cell 20(5):661-73. [PubMed: 22094259] [MGI Ref ID J:178950]
Schemionek M; Elling C; Steidl U; Baumer N; Hamilton A; Spieker T; Gothert JR; Stehling M; Wagers A; Huettner CS; Tenen DG; Tickenbrock L; Berdel WE; Serve H; Holyoake TL; Muller-Tidow C; Koschmieder S. 2010. BCR-ABL enhances differentiation of long-term repopulating hematopoietic stem cells. Blood 115(16):3185-95. [PubMed: 20053753] [MGI Ref ID J:160797]
Sengupta A; Arnett J; Dunn S; Williams DA; Cancelas JA. 2010. Rac2 GTPase deficiency depletes BCR-ABL+ leukemic stem cells and progenitors in vivo. Blood 116(1):81-4. [PubMed: 20407032] [MGI Ref ID J:162808]
Sengupta A; Ficker AM; Dunn SK; Madhu M; Cancelas JA. 2012. Bmi1 reprograms CML B-lymphoid progenitors to become B-ALL-initiating cells. Blood 119(2):494-502. [PubMed: 22101899] [MGI Ref ID J:181793]
Zhang B; Ho YW; Huang Q; Maeda T; Lin A; Lee SU; Hair A; Holyoake TL; Huettner C; Bhatia R. 2012. Altered microenvironmental regulation of leukemic and normal stem cells in chronic myelogenous leukemia. Cancer Cell 21(4):577-92. [PubMed: 22516264] [MGI Ref ID J:189328]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice are bred together or to wildtype siblings.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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