Strain Name:

FVB.Cg-Tg(Tal1-tTA)19Dgt/J

Stock Number:

006209

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN7+N1F2pN1
Generation Definitions
 
Donating Investigator Daniel G. Tenen,   Beth Israel Deaconess Medical Center

Description
Hemizygotes are viable, fertile, normal in size, and do not display any behavioral abnormalities. Hemizygotes express tetracycline-controlled transactivator protein (tTA) in bone marrow hematopoietic stem cells (HSC) and in common myeloid progenitors (CMP)). tTA activity also is detected in lung. Little to no tTA activity is detected in thymus, lymph node, intestine or spleen. When bred to other transgenic mice carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring can be induced by withdrawal of tetracycline or doxycycline. This strain represents an effective tool for generating bitrangenic animals to study inducible gene expression in blood stem and progenitor cells.

These mice were originally designed to be bred with transgenic mice harboring a TRE-BCR-ABL1 transgene (Stock No. 006202), creating bitransgenic offspring as a model for studies of the Philadelphia chromosome and inducible chronic myeloid leukemia.

Development
A transgenic construct consisting of the SV40 minimal promoter, rabbit beta-globin intron 2, and the 3' enhancer sequence from the murine T-cell acute lymphocytic leukemia 1 gene (Tal1, formerly called homolog of human stem cell leukemia gene or SCL) was designed to direct expression of the tetracycline-regulated transactivator, tTA. The construct was injected into the pronucleus of "BDF" (C57BL/6 x DBA/2 F1 hybrid) eggs, which subsequently were implanted into pseudopregnant foster mothers. Transgenic mice were bred to generate founder line 19. In 2004, mice were bred to a "tetO-cre" transgenic mouse on an unidentified background. Subsequently, mice were selected for the Tal1-tTA transgene (and against tetO-cre) while backcrossing to FVB/N for approximately 7-9 generations prior to arrival at the Jackson Laboratory. The donating investigators note that tissues from these mice can transplant into FVB/N mice without rejection.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Tal1-tTA)19Dgt allele
017722   B6.Cg-Tg(Tal1-tTA)19Dgt/J
View Strains carrying   Tg(Tal1-tTA)19Dgt     (1 strain)

View Strains carrying other alleles of SV40     (12 strains)

Strains carrying other alleles of tTA
008079   129S-Ppargtm2Yba/J
016198   129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ
011008   B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
009602   B6.129S4(Cg)-Kcnn2tm2Jpad/J
009603   B6.129S4-Kcnn3tm1Jpad/J
008227   B6.129S4-Ppargtm3Yba/J
012359   B6.Cg-Pvalbtm1.1(tTA2)Hze/J
016868   B6.Cg-Ssttm1.2(tTA2)Hze/J
007004   B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
003563   B6.Cg-Tg(Cebpb-tTA)5Bjd/J
003767   B6.Cg-Tg(Eno2tTA)5021Nes/J
003763   B6.Cg-Tg(Eno2tTA)5030Nes/J
018306   B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
005964   B6.Cg-Tg(GFAP-tTA)110Pop/J
002618   B6.Cg-Tg(MMTVtTA)1Mam/J
008284   B6.Cg-Tg(Scg2-tTA)1Jt/J
023970   B6.Cg-Tg(Sirpa-tTA)AUmri/J
023971   B6.Cg-Tg(Sirpa-tTA)SUmri/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
017754   B6;129-Omptm1(tTA)Gogo/J
007585   B6;129S4-Npytm2Rpa/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
003010   B6;CBA-Tg(Camk2a-tTA)1Mmay/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
010573   B6;SJL-Tg(Prl-tTA)6-5Jek/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
008603   C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J
010712   C57BL/6-Tg(Camk2a-tTA)1Stl/J
013585   FVB-Tg(Cdh5-tTA)D5Lbjn/J
005625   FVB-Tg(Pcp2-tTA)3Horr/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
005942   FVB/N-Tg(Pf4-tTA/VP16)42Kra/J
004937   NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J
008335   STOCK Foxa2tm1.1(rtTa)Moon/J
008600   STOCK Gt(ROSA)26Sortm1(tTA)Roos/J
005701   STOCK Pdx1tm1Macd/J
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
003271   STOCK Tg(CMV-tTA)3Bjd/J
024854   STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)#Kha/J
018124   STOCK Tg(Prnp-tTA)F959Sbp/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
003275   STOCK Tg(tetL)1Bjd/J
003274   STOCK Tg(tetNZL)2Bjd/J
016970   STOCK Tg(tetO-HCV)1Mlch/Mmjax
View Strains carrying other alleles of tTA     (45 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Chronic Myelogenous Leukemia
Increased Tumor Incidence
      Leukemia
      Leukemia: lymphocytic

Developmental Biology Research
Internal/Organ Defects
      hematopoietic defects
Lymphoid Tissue Defects
      hematopoietic defects

Hematological Research
Hematopoietic Defects

Immunology, Inflammation and Autoimmunity Research
Lymphoid Tissue Defects

Research Tools
Cancer Research
      Leukemia
      Tetop Tet System
      myeloma and hybridoma production
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Tetop Tet System
Hematological Research
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Tal1-tTA)19Dgt
Allele Name transgene insertion 19, Daniel G Tenen
Allele Type Transgenic (random, expressed)
Common Name(s) SCLtTA; SCLtTA stg; Scl-tTA;
Mutation Made By Daniel Tenen,   Beth Israel Deaconess Medical Center
Strain of Origin(C57BL/6 x DBA/2)F1
Site of ExpressionExpresses tTA in bone marrow-derived hematopoietic stem cells (HSC) and common myeloid progenitors (CMP). tTA activity also is detected in lung.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter SV40, E mu enhancer, SV40
Molecular Note The transgene consists of the SV40 minimal promoter, rabbit beta-globin intron 2, and the 3' enhancer sequence from the mouse T-cell acute lymphocytic leukemia gene driving expression of the tetracycline-regulated transactivator. [MGI Ref ID J:96511]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Tal1-tTA)19Dgt, Separated PCR
Tg(tTA), Melt Curve Analysis
Tg(tTA), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Koschmieder S; Gottgens B; Zhang P; Iwasaki-Arai J; Akashi K; Kutok JL; Dayaram T; Geary K; Green AR; Tenen DG; Huettner CS. 2005. Inducible chronic phase of myeloid leukemia with expansion of hematopoietic stem cells in a transgenic model of BCR-ABL leukemogenesis. Blood 105(1):324-34. [PubMed: 15331442]  [MGI Ref ID J:96511]

Additional References

Tg(Tal1-tTA)19Dgt related

Bolton-Gillespie E; Schemionek M; Klein HU; Flis S; Hoser G; Lange T; Nieborowska-Skorska M; Maier J; Kerstiens L; Koptyra M; Muller MC; Modi H; Stoklosa T; Seferynska I; Bhatia R; Holyoake TL; Koschmieder S; Skorski T. 2013. Genomic instability may originate from imatinib-refractory chronic myeloid leukemia stem cells. Blood 121(20):4175-83. [PubMed: 23543457]  [MGI Ref ID J:198207]

Chen CI; Koschmieder S; Kerstiens L; Schemionek M; Altvater B; Pscherer S; Gerss J; Maecker HT; Berdel WE; Juergens H; Lee PP; Rossig C. 2012. NK cells are dysfunctional in human chronic myelogenous leukemia before and on imatinib treatment and in BCR-ABL-positive mice. Leukemia 26(3):465-74. [PubMed: 21904381]  [MGI Ref ID J:181529]

Eiring AM; Harb JG; Neviani P; Garton C; Oaks JJ; Spizzo R; Liu S; Schwind S; Santhanam R; Hickey CJ; Becker H; Chandler JC; Andino R; Cortes J; Hokland P; Huettner CS; Bhatia R; Roy DC; Liebhaber SA; Caligiuri MA; Marcucci G; Garzon R; Croce CM; Calin GA; Perrotti D. 2010. miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts. Cell 140(5):652-65. [PubMed: 20211135]  [MGI Ref ID J:160777]

Hamilton A; Helgason GV; Schemionek M; Zhang B; Myssina S; Allan EK; Nicolini FE; Muller-Tidow C; Bhatia R; Brunton VG; Koschmieder S; Holyoake TL. 2012. Chronic myeloid leukemia stem cells are not dependent on Bcr-Abl kinase activity for their survival. Blood 119(6):1501-10. [PubMed: 22184410]  [MGI Ref ID J:181724]

Harb JG; Neviani P; Chyla BJ; Ellis JJ; Ferenchak GJ; Oaks JJ; Walker CJ; Hokland P; Roy DC; Caligiuri MA; Marcucci G; Huettner CS; Perrotti D. 2013. Bcl-xL anti-apoptotic network is dispensable for development and maintenance of CML but is required for disease progression where it represents a new therapeutic target. Leukemia 27(10):1996-2005. [PubMed: 23670294]  [MGI Ref ID J:201846]

Moore CR; Liu Y; Shao C; Covey LR; Morse HC 3rd; Xie P. 2012. Specific deletion of TRAF3 in B lymphocytes leads to B-lymphoma development in mice. Leukemia 26(5):1122-7. [PubMed: 22033491]  [MGI Ref ID J:188983]

Reynaud D; Pietras E; Barry-Holson K; Mir A; Binnewies M; Jeanne M; Sala-Torra O; Radich JP; Passegue E. 2011. IL-6 controls leukemic multipotent progenitor cell fate and contributes to chronic myelogenous leukemia development. Cancer Cell 20(5):661-73. [PubMed: 22094259]  [MGI Ref ID J:178950]

Schemionek M; Elling C; Steidl U; Baumer N; Hamilton A; Spieker T; Gothert JR; Stehling M; Wagers A; Huettner CS; Tenen DG; Tickenbrock L; Berdel WE; Serve H; Holyoake TL; Muller-Tidow C; Koschmieder S. 2010. BCR-ABL enhances differentiation of long-term repopulating hematopoietic stem cells. Blood 115(16):3185-95. [PubMed: 20053753]  [MGI Ref ID J:160797]

Schepers K; Pietras EM; Reynaud D; Flach J; Binnewies M; Garg T; Wagers AJ; Hsiao EC; Passegue E. 2013. Myeloproliferative neoplasia remodels the endosteal bone marrow niche into a self-reinforcing leukemic niche. Cell Stem Cell 13(3):285-99. [PubMed: 23850243]  [MGI Ref ID J:201668]

Sengupta A; Arnett J; Dunn S; Williams DA; Cancelas JA. 2010. Rac2 GTPase deficiency depletes BCR-ABL+ leukemic stem cells and progenitors in vivo. Blood 116(1):81-4. [PubMed: 20407032]  [MGI Ref ID J:162808]

Sengupta A; Ficker AM; Dunn SK; Madhu M; Cancelas JA. 2012. Bmi1 reprograms CML B-lymphoid progenitors to become B-ALL-initiating cells. Blood 119(2):494-502. [PubMed: 22101899]  [MGI Ref ID J:181793]

Zhang B; Ho YW; Huang Q; Maeda T; Lin A; Lee SU; Hair A; Holyoake TL; Huettner C; Bhatia R. 2012. Altered microenvironmental regulation of leukemic and normal stem cells in chronic myelogenous leukemia. Cancer Cell 21(4):577-92. [PubMed: 22516264]  [MGI Ref ID J:189328]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, transgenic mice are bred together or to wildtype siblings.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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