Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S1-Xlkd1tm1Lhua/J    (Changed: 06-JUL-07 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N6+N1F3pN1 Generation Definitions   Donating Investigator Jung Huang,   St. Louis University School of Medicine

Description
Homozygotes are viable and fertile, and produce normal-sized litter. No gross phenotypic or behavioral abnormalities have been reported, even in older (2 year old) mice. Homozygous mutants express neither endogenous RNA or protein in liver tissue. Lymphatic capillary vessel morphology in the liver and intestines of homozygous mice is abnormal, with vessels having distended or rounded lumens in contrast to the smaller, typically collapsed, irregular shapes observed in wildtype controls. Intradermal interstitial-lymphatic flow also is increased. Syngenic tumor cell transplants into grow more rapidly and robustly in homozygous mutant mice compared with transplants into wildtype mice, and develop porous interstitial spaces. These mutant mice may be useful in studies of structural and functional characteristics of the lymphatic system, cell-surface retention sequence (CRS) motif-containing growth factor secretion, autocrine and paracrine regulation of cell growth, as well as of cancer and the mechanisms of metastasis.

Development
A targeting vector was designed to replace a portion of exon 2 and all of exon 3 of the endogenous gene with a male-germline, self-excising loxP-flanked cassette (itself containing a neomycin resistance (Neo) gene and a testes-specific angiotensin-converting enzyme (tACE) promoter-driven Cre recombinase gene). The construct was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES clones were injected into C57BL/6J blastocysts and chimeric males were backcrossed for germ-line transmission to C57BL/6J females. The resulting cassette-excised offspring (containing a single loxP site in place of the 3' region of exon 2 and all of exon 3) were backcrossed for 6 generations onto the C57BL/6 genetic background prior to arrival at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Lyve1

012601

B6;129P2-Lyve1tm1.1(EGFP/cre)Cys/J

View Strains carrying other alleles of Lyve1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Lyve1^tm1Lhua/Lyve1^tm1Lhua

        B6.129S1-Lyve1^tm1Lhua

• immune system phenotype
• abnormal lymph circulation
  • intradermally injected FITC-dextran moves away from the injection site more rapidly suggesting increased interstitial-lymphatic flow   (MGI Ref ID J:117713)
• lymphangiectasis
  • vessels in the liver and intestine, but not in other tissues, display distended lumens with extended or round shapes   (MGI Ref ID J:117713)
• cardiovascular system phenotype
• abnormal lymph circulation
  • intradermally injected FITC-dextran moves away from the injection site more rapidly suggesting increased interstitial-lymphatic flow   (MGI Ref ID J:117713)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Other
      tumor metastasis

Cardiovascular Research
Vascular Defects

Developmental Biology Research
Internal/Organ Defects
      Lymphoid Tissue Defects
Lymphoid Tissue Defects

Immunology and Inflammation Research
Lymphoid Tissue Defects

Internal/Organ Research
Gastrointestinal Defects
Liver Defects
Lymphoid Tissue Defects

Research Tools
Cancer Research
      tumor immunology
      xenograft/transplant host
Cardiovascular Research
      endothelial cell marker for neovascularization
Internal/Organ Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Lyve1tm1Lhua
Allele Name		targeted mutation 1, Jung S Huang
Allele Type		Targeted (knock-out)
Common Name(s)		Crsbp-1-;
Strain of Origin		129S1
ES Cell Line Strain		129
Gene Symbol and Name		Lyve1, lymphatic vessel endothelial hyaluronan receptor 1
Chromosome		7
Gene Common Name(s)		1200012G08Rik; CRSBP-1; HAR; LYVE-1; RIKEN cDNA 1200012G08 gene; XLKD1; Xlkd1; extra cellular link domain-containing 1; lymphatic vessel endothelial HA receptor-1;
Molecular Note		A targeting vector was designed to remove parts of exon 2, intron 2, exon 3 and intron 3 of the gene, and also to create a frame shift. The vector contained a floxed cassette containing a neomycin resistance gene and a testes specific angiotensin-converting enzyme promoter driving expression of cre recombinase. The floxed cassette was excised from the locus upon passage through the male germline. [MGI Ref ID J:117713]

Genotyping

Genotyping Information

Genotyping Protocols

Lyve1^tm1Lhua, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Huang SS; Liu IH; Smith T; Shah MR; Johnson FE; Huang JS. 2006. CRSBP-1/LYVE-l-null mice exhibit identifiable morphological and functional alterations of lymphatic capillary vessels. FEBS Lett 580(26):6259-68. [PubMed: 17070806]  [MGI Ref ID J:117713]

Additional References

Lyve1^tm1Lhua related

Yan A; Avraham T; Zampell JC; Aschen SZ; Mehrara BJ. 2011. Mechanisms of lymphatic regeneration after tissue transfer. PLoS One 6(2):e17201. [PubMed: 21359148]  [MGI Ref ID J:171084]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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