Strain Name:

B6.129S1-Nox3het-3J/GrsrJ

Stock Number:

006228

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Chemically Induced Mutation; Congenic; Mutant Strain;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationF15pN1
Generation Definitions

Description
Homozygotes completely lack otoconia, both in the utricle and the saccule, as early as embryonic day 14 and this persists in the adult. However, the rest of the inner ear appears morphologically normal and hearing is normal. Otoconia appear to be normal in heterozygotes. Homozygotes can be identified by a significant sideways tilting of the head. When dropped 30cm to a soft surface homozygotes fail to land on their feet. They also fail to orient and swim in water, but instead rotate underwater and require rescue.

Development
The head tilt 3 Jackson mutation was generated in an EMS mutagenesis screen. 129S1/SvImJ-derived CJ7 ES cells were treated with ethylmethanesulphonate and injected into C57BL/6J blastocysts to generate chimeras which were bred with C57BL/6J and the head tilt 3 Jackson mutation was identified in subsequent screening progeny. Backcrossing to C57BL/6J continued, resulting in this congenic which reached generation N15 in 2010.

Related Strains

Strains carrying other alleles of Nox3
003483   B6 x B10.D1-H2q/SgJ-Nox3het-2J/J
000061   B6 x STOCK Nox3het/J
002557   B6Ei.GL-Nox3het/J
005014   C57BL/6J-Nox3het-4J/J
View Strains carrying other alleles of Nox3     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Nox3het-3J/Nox3het-3J

        involves: 129S1/Sv * C57BL/6J
  • behavior/neurological phenotype
  • abnormal postural reflex
    • mutants have severely impaired postural reflexes   (MGI Ref ID J:89010)
  • head tilt   (MGI Ref ID J:89010)
  • impaired balance
    • mutants have severely reduced performance in the stationary beam test   (MGI Ref ID J:89010)
  • impaired coordination
    • mutants have severely reduced performance in the coat hanger test   (MGI Ref ID J:89010)
  • impaired swimming
    • mutants are unable to swim or float   (MGI Ref ID J:89010)
  • hearing/vestibular/ear phenotype
  • absent otoliths
    • the otoliths are bilaterally absent   (MGI Ref ID J:89010)
    • mutants displayed normal response to auditory stimuli   (MGI Ref ID J:89010)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Nox3het-3J related

Sensorineural Research
Vestibular Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nox3het-3J
Allele Name head tilt 3 Jackson
Allele Type Chemically induced (other)
Common Name(s) vst;
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Nox3, NADPH oxidase 3
Chromosome 17
Gene Common Name(s) GP91-3; MOX-2; head-tilt; het; neuroscience mutagenesis facility, 250; nmf250;
Molecular Note A G to A transition at the -1 position of the spice acceptor site just prior to exon 2 is predicted to result in the introduction of six novel amino acids prior to a premature stop codon, deletion of amino acids 37-68, and the rest of the protein sequence remaining in frame. No complementation was seen among offspring in crosses between heterozygotes of this allele and homozygotes of head tilt or head tilt 2 Jackson. [MGI Ref ID J:169054] [MGI Ref ID J:89010]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Munroe RJ; Bergstrom RA; Zheng QY; Libby B; Smith R; John SW; Schimenti KJ; Browning VL; Schimenti JC. 2000. Mouse mutants from chemically mutagenized embryonic stem cells. Nat Genet 24(3):318-21. [PubMed: 10700192]  [MGI Ref ID J:60747]

Paffenholz R; Bergstrom RA; Pasutto F; Wabnitz P; Munroe RJ; Jagla W; Heinzmann U; Marquardt A; Bareiss A; Laufs J; Russ A; Stumm G; Schimenti JC; Bergstrom DE. 2004. Vestibular defects in head-tilt mice result from mutations in Nox3, encoding an NADPH oxidase. Genes Dev 18(5):486-91. [PubMed: 15014044]  [MGI Ref ID J:89010]

Additional References

Nox3het-3J related

Flaherty JP; Fairfield HE; Spruce CA; McCarty CM; Bergstrom DE. 2010. Molecular characterization of an allelic series of mutations in the mouse Nox3 gene. Mamm Genome :. [PubMed: 21161235]  [MGI Ref ID J:169054]

Monroe RJ; Bergstrom DE; Schimenti JC. 2002. A new allele at the head-tilt (het) locus MGI Direct Data Submission :.  [MGI Ref ID J:76316]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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