Strain Name: |
B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J |
|---|---|
Stock Number: |
006230 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Cebpa; Cebpatm1Dgt; Mx1; cre; Tg(Mx1-cre)1Cgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Mating System See Colony Maintenance (Female x Male) Species laboratory mouse Donating Investigator Daniel Tenen, Beth Israel Deaconess Medical Center Generation N9PN2f2 (28-APR-08) Strain Description
Mice homozygous for this C/EBPalpha "floxed" allele (C/EBPalphaF) and hemizygous for the Mx1-cre transgene are viable and fertile, and exhibit no abnormalities in the hematopoietic system. In the absence of cre expression, the C/EBPalphaF allele functions similarly to the wildtype allele. Mx1-Cre transgene expression can be induced by administration of either interferon (alpha or beta) or synthetic double-stranded RNA (such as poly I:C), leading to deletion of the "floxed" gene. Following 3-4.5 weeks of poly I:C treatment, deletion efficiency is greater than 95% in hematopoietic tissues, and C/EBPalpha protein is undetectable in bone marrow. These poly I:C-treated, mice have defective myeloid cell development, increased hematopoietic stem cell repopulating activity, and a significantly increased myeloblast population in the bone marrow compartment. These mutant mice may be useful in studies of hematopoietic cell (e.g. myeloid and basophil progenitor cell) development and function, cancer (e.g. acute myeloid leukemia), and alveolar cell differentiation.Strain Development
These mice contain the C/EBPalphaF targeted mutation and the Mx1-Cre transgene. To generate the C/EBPalphaF mutant mice, a targeting vector was designed to flank the single Cebpa exon with loxP sites. A neomycin resistance cassette was also inserted between the exon and downstream loxP site. This vector was introduced into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted cells were injected into C57BL/6 blastocysts and then implanted into foster mothers. Mutant offspring were backcrossed for 9 generations to C57BL/6 mice. The Mx1-Cre transgenic construct was designed with the mouse Mx1 promoter, nuclear localization sequence-modified Cre recombinase, and a 2.1 kb fragment from the human growth hormone gene (to provide the splicing and polyadenylation signals). Transgenic mice were generated on a mixed CBA and C57BL/6 genetic background by Dr. Klaus Rajewsky (Harvard Medical School). Transgenic mice were sent to Dr. Daniel Tenen (Harvard Medical School) and then backcrossed for 9 generations to C57BL/6 mice. After backcrossing, the C/EBPalphaF mutant and Mx1-Cre transgenic strains were bred together in the laboratory of Dr. Tenen for many generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Cebpatm1Dgt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Daniel G Tenen | ||
| Common Name(s) | C/EBPalphaF; | ||
| Mutation Made By | Daniel Tenen, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | TC-1 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Gene Symbol and Name | Cebpa, CCAAT/enhancer binding protein (C/EBP), alpha | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | C/EBP alpha; C/EBP-alpha; CCAAT/enhancer binding protein (C/EBP); CEBP; Cebp; DBPCEP; | ||
| Molecular Note | LoxP sites were inserted to flank the single exon and a neo cassette inserted downstream of the exon. Western blot of whole-cell lysates of adult liver and total bone marrow cells showed that protein was present in mutants. [J:94682] | ||
| Allele Symbol | Tg(Mx1-cre)1Cgn | ||
| Allele Name | transgene insertion 1, University of Cologne | ||
| Common Name(s) | Mx-Cre; Mx-Cre 31; Mx1-Cre; Mx1cre; MxCre; | ||
| Mutation Made By | Ralf Kuhn, University of Cologne | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | widespread pattern of expression; promoter induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA; provides capability to induce the "knockout" at any time during development | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Mx1, myxovirus (influenza virus) resistance 1, mouse, laboratory | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of an inducible Mx1 promoter, which is silent in healthy mice, and active in the liver and in lymphocytes after induction with IFN or pI-pC. The construct also contains a 2.1 kb fragment from thehuman growth hormone gene. [J:105040] [J:67927] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Cebpatm1Dgt
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, mice homozygous for the "floxed" mutation and hemizygous for the transgene are bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Cebpatm1Dgt allele
006447 B6.129S6(CBA)-Cebpatm1Dgt/J View Strains carrying Cebpatm1Dgt (1 strain)
003556 B6.Cg-Tg(Mx1-cre)1Cgn/J 005673 C.Cg-Tg(Mx1-cre)1Cgn/J 004192 STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J 002527 STOCK Tg(Mx1-cre)1Cgn/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedCancer Research
Increased Tumor Incidence (Leukemia: lymphocytic)
Cell Biology Research
Transcriptional Regulation
Developmental Biology Research
Lymphoid Tissue Defects (hematopoietic defects)
Hematological Research
Hematopoietic Defects
Immunological Defects
Immunology and Inflammation Research
Lymphoid Tissue Defects (hematopoietic development)
Lymphoid Tissue Defects (myeloid hyperplasia)
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
Cancer Research (Leukemia)
Cancer Research (myeloma and hybridoma production)
Cre-lox-System (loxP-flanked Sequences)
Cre-lox-System (Cre-Recombinase Expression: Inducible)
Hematological Research
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesZhang P; Iwasaki-Arai J; Iwasaki H; Fenyus ML; Dayaram T; Owens BM; Shigematsu H; Levantini E; Huettner CS; Lekstrom-Himes JA; Akashi K; Tenen DG. 2004. Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha. Immunity 21(6):853-63. [PubMed: 15589173] [J:94682]
Price and Supply Information
| Strain Name: | B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J |
| Stock Number: | 006230 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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