Strain Name: |
B6.Cg-Tg(tetO-cre)1Jaw/J |
|---|---|
Stock Number: |
006234 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | cre; Tg(tetO-cre)1Jaw; tetO; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x Inbred (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N4 (08-JAN-08) Strain Description
Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the control of tissue-specific promoters, Cre recombinase expression and Cre-mediated recombination in the appropriate tissues of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline. This strain represents an effective tool for generating inducible tissue specific-targeted mutants to study cell lineage during development.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing the cre recombinase gene under the control of the tetracycline-responsive promoter element (TRE or tetO), minimal CMV promoter and MT-1 polyadenylation site sequence was injected into mixed C57BL/6 and 129 background embryos. Founder mice were bred to FVB/N animals. Upon arrival at The Jackson Laboratory, these mice were backcrossed to C57BL/6J mice for at least 5 generations.
| Allele Symbol | Tg(tetO-cre)1Jaw | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jeffrey A Whitsett | ||
| Common Name(s) | (tetO)7-CMV-Cre; (tetO)7-CMV-Cretg; (tetO)7-Cre; (tetO)7CMVtg; TRECre; tetO-cre; | ||
| Mutation Made By | Andras Nagy, Mount Sinai Hospital | ||
| Strain of Origin | 129 and C57BL/6 | ||
| Site of Expression | widespread, inducible expression of Cre recombinase | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | tetO, tet operator, | ||
| Molecular Note | The transgene is composed of 7 tetO sites, a minimal CMV promoter, and sequence encoding cre recombinase. [MGI Ref ID J:78365] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Generic Cre
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Strains carrying Tg(tetO-cre)1Jaw allele
006244 C.Cg-Tg(tetO-cre)1Jaw/J 008244 FVB.Cg-Tg(tetO-cre)1Jaw/J 006224 STOCK Tg(tetO-cre)1Jaw/J View Strains carrying Tg(tetO-cre)1Jaw (3 strains)
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (109 strains)
Strains carrying other alleles of tetO
View Strains carrying other alleles of tetO (23 strains)
Congenic Nomenclature
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Cre-lox System (Cre-Recombinase Expression: Inducible)
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Selected Reference(s)
Additional ReferencesPerl AK; Wert SE; Nagy A; Lobe CG; Whitsett JA. 2002. Early restriction of peripheral and proximal cell lineages during formation of the lung. Proc Natl Acad Sci U S A 99(16):10482-7. [PubMed: 12145322] [MGI Ref ID J:78365]
| Strain Name: | B6.Cg-Tg(tetO-cre)1Jaw/J |
| Stock Number: | 006234 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 18-AUG-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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