Strain Name:

B6.129S6-Casp7tm1Flv/J

Stock Number:

006237

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Availability:

Repository- Live

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Mouse embryonic fibroblasts from knock-out homozygotes exhibit a slight survival advantage when treated with apoptosis inducers. These mutant mice may be useful in studies of mitochondrial events of apoptosis, especially when paired with other executioner caspase mutant models.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   27-JAN-07
Specieslaboratory mouse
GenerationN10+N1F1 (11-DEC-13)
Generation Definitions
 
Donating InvestigatorDr. Richard A. Flavell,   Yale University School of Medicine

Description
Homozygous mice are viable and fertile with normal appearance, organ morphology, and lymphoid development. Endogenous protein expression is absent in all tissues tested (including brain, thymus, heart, lung, liver, spleen, kidney, and skeletal muscle). Mouse embryonic fibroblasts (MEFs) from homozygotes exhibit a slight survival advantage when treated with apoptosis inducers, but other cell subsets undergo normal apoptosis (including activated T cells death following T cell receptor stimulation, thymocyte apoptosis, and Fas-mediated B cell and hepatocyte cell death). These mutant mice may be useful in studies of mitochondrial events of apoptosis, especially when paired with other executioner caspase mutant models.

Development
A targeting vector was designed to replace exons 2 and 3 of the endogenous gene with a neomycin resistance cassette and to introduce a frame-shift mutation in the remaining downstream sequence. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. The donating investigator reports that heterozygous mice were backcrossed to C57BL/6 mice for 10 generations before arrival at The Jackson Laboratory (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. One of these markers that was not segregating is found on Chromosome 19, where Casp7 resides. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Casp7tm1Flv/Casp7tm1Flv

        B6.129S6-Casp7tm1Flv
  • cellular phenotype
  • decreased apoptosis
    • mouse embryonic fibroblasts (MEFs) exhibit a slight decrease in apoptosis rates following exposure to various insults   (MGI Ref ID J:105496)
    • following UV irradiation and staurosporine treatment, MEFs exhibit increased survival compared to wild type or Casp7tm1Flv MEFs   (MGI Ref ID J:105496)
  • immune system phenotype
  • *normal* immune system phenotype
    • lymphoid development and T cell apoptosis are normal   (MGI Ref ID J:105496)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Casp7tm1Flv/Casp7tm1Flv

        Background Not Specified
  • vision/eye phenotype
  • *normal* vision/eye phenotype
    • lenses appear grossly normal   (MGI Ref ID J:101037)
    • lens extracts display normal levels of lysis of fluorogenic substrate DEVD-AMC   (MGI Ref ID J:101037)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apoptosis Research
Endogenous Regulators

Immunology, Inflammation and Autoimmunity Research
Intracellular Signaling Molecules

Research Tools
Apoptosis Research
Immunology, Inflammation and Autoimmunity Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Casp7tm1Flv
Allele Name targeted mutation 1, Richard A Flavell
Allele Type Targeted (Null/Knockout)
Common Name(s) Casp7-;
Mutation Made ByDr. Richard Flavell,   Yale University School of Medicine
Strain of Origin129S6/SvEvTac
ES Cell Line NameTC1/TC-1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Casp7, caspase 7
Chromosome 19
Gene Common Name(s) AI314680; CASP-7; CMH-1; ICE-IAP3; ICE-LAP3; LICE2; MCH3; caspase-7; expressed sequence AI314680;
Molecular Note The targeting construct replaces exons 2 and 3 with a neomycin resistance cassette, and also results in a frame-shift. Western blot analysis showed absence of caspase 7 expression in homozygous mice. [MGI Ref ID J:105496]

Genotyping

Genotyping Information

Genotyping Protocols

Casp7tm1Flv, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Casp7tm1Flv related

Akhter A; Gavrilin MA; Frantz L; Washington S; Ditty C; Limoli D; Day C; Sarkar A; Newland C; Butchar J; Marsh CB; Wewers MD; Tridandapani S; Kanneganti TD; Amer AO. 2009. Caspase-7 activation by the Nlrc4/Ipaf inflammasome restricts Legionella pneumophila infection. PLoS Pathog 5(4):e1000361. [PubMed: 19343209]  [MGI Ref ID J:162695]

Guo YH; Hernandez I; Isermann B; Kang TB; Medved L; Sood R; Kerschen EJ; Holyst T; Mosesson MW; Weiler H. 2009. Caveolin-1-dependent apoptosis induced by fibrin degradation products. Blood 113(18):4431-9. [PubMed: 19074731]  [MGI Ref ID J:148438]

Josefsson EC; Burnett DL; Lebois M; Debrincat MA; White MJ; Henley KJ; Lane RM; Moujalled D; Preston SP; O'Reilly LA; Pellegrini M; Metcalf D; Strasser A; Kile BT. 2014. Platelet production proceeds independently of the intrinsic and extrinsic apoptosis pathways. Nat Commun 5:3455. [PubMed: 24632563]  [MGI Ref ID J:210320]

Ko MK; Saraswathy S; Parikh JG; Rao NA. 2011. The role of TLR4 activation in photoreceptor mitochondrial oxidative stress. Invest Ophthalmol Vis Sci 52(8):5824-35. [PubMed: 21666244]  [MGI Ref ID J:181405]

Lakhani SA; Masud A; Kuida K; Porter GA Jr; Booth CJ; Mehal WZ; Inayat I; Flavell RA. 2006. Caspases 3 and 7: key mediators of mitochondrial events of apoptosis. Science 311(5762):847-51. [PubMed: 16469926]  [MGI Ref ID J:105496]

Lamkanfi M; Moreira LO; Makena P; Spierings DC; Boyd K; Murray PJ; Green DR; Kanneganti TD. 2009. Caspase-7 deficiency protects from endotoxin-induced lymphocyte apoptosis and improves survival. Blood 113(12):2742-5. [PubMed: 19168786]  [MGI Ref ID J:146876]

Lamkanfi M; Sarkar A; Vande Walle L; Vitari AC; Amer AO; Wewers MD; Tracey KJ; Kanneganti TD; Dixit VM. 2010. Inflammasome-dependent release of the alarmin HMGB1 in endotoxemia. J Immunol 185(7):4385-92. [PubMed: 20802146]  [MGI Ref ID J:164216]

Vince JE; Wong WW; Gentle I; Lawlor KE; Allam R; O'Reilly L; Mason K; Gross O; Ma S; Guarda G; Anderton H; Castillo R; Hacker G; Silke J; Tschopp J. 2012. Inhibitor of Apoptosis Proteins Limit RIP3 Kinase-Dependent Interleukin-1 Activation. Immunity 36(2):215-27. [PubMed: 22365665]  [MGI Ref ID J:181625]

Ye J; Llorian M; Cardona M; Rongvaux A; Moubarak RS; Comella JX; Bassel-Duby R; Flavell RA; Olson EN; Smith CW; Sanchis D. 2013. A pathway involving HDAC5, cFLIP and caspases regulates expression of the splicing regulator polypyrimidine tract binding protein in the heart. J Cell Sci 126(Pt 7):1682-91. [PubMed: 23424201]  [MGI Ref ID J:200588]

Zandy AJ; Lakhani S; Zheng T; Flavell RA; Bassnett S. 2005. Role of the executioner caspases during lens development. J Biol Chem 280(34):30263-72. [PubMed: 15994297]  [MGI Ref ID J:101037]

Zheng TS; Hunot S; Kuida K; Flavell RA. 1999. Caspase knockouts: matters of life and death Cell Death Differ 6(11):1043-53. [PubMed: 10578172]  [MGI Ref ID J:59875]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   27-JAN-07
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Casp7tm1Flv  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Casp7tm1Flv x Homozygous for Casp7tm1Flv  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Casp7tm1Flv  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Casp7tm1Flv x Homozygous for Casp7tm1Flv  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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