Strain Name:

129-Wnt11tm1Amc/J

Stock Number:

006239

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These mice harbor a "knock-out" mutation of the Wnt11 (wingless-related MMTV integration site 11) gene and may be useful in studies of kidney development (including ureteric bud branching morphogenesis) and Wnt superfamily embryogenesis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN1pN1
Generation Definitions
 
Donating Investigator Andrew P McMahon,   University of Southern California

Description
Mice heterozygous for this targeted mutation are viable and fertile with normal kidney size and histology. Homozygotes exhibit some embryonic lethality and will die by 2 days post partem. While the cause of death is unclear, these neonates have kidney hypoplasia and reduction of glomeruli. RT-PCR analysis of kidney RNA shows the expected truncated transcript. Homozygotes exhibit ureteric branching morphogenesis defects between embryonic day 11.5-12.5 (T-stage) associated with a reduction in mesenchymal Gdnf expression. These Wnt11 mutant mice may be useful in studies of kidney development, including ureteric bud branching morphogenesis, and Wnt superfamily embryogenesis.

Development
A targeting vector was designed to replace exons 4 and 5 of the endogenous gene with a PGK-neo cassette. This construct was microinjected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric mice were bred to "129 substrain AV3" inbred mice (129X1/SvJ). Heterozygous mice were maintained and expanded on this same genetic background (129X1/SvJ) prior to arrival at The Jackson Laboratory. Upon arrival, these mice were bred to 129X1/SvJ (Stock No. 000691) inbred mice for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
   000691 129X1/SvJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Wnt11
018683   B6;D-Tg(Wnt11-TagRFP/cre/ERT2)28Amc/J
View Strains carrying other alleles of Wnt11     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Wnt11tm1Amc/Wnt11tm1Amc

        involves: 129S1/Sv * 129X1/SvJ
  • mortality/aging
  • complete neonatal lethality
    • mutants surviving to birth die within 2 days post-partum   (MGI Ref ID J:83430)
  • partial embryonic lethality during organogenesis
    • significant number of mutants die by E12.5   (MGI Ref ID J:83430)
  • renal/urinary system phenotype
  • abnormal ureteric bud tip morphology
    • at E12.5, there is loss of ureteric tips and some ampullae have abnormal morphology   (MGI Ref ID J:83430)
  • decreased renal glomerulus number
    • kidneys of newborn mice have 64% the number of glomeruli found in wild-type   (MGI Ref ID J:83430)
  • delayed kidney development   (MGI Ref ID J:83430)
  • impaired branching involved in ureteric bud morphogenesis
    • delayed kidney development resulting in abnormal ureteric branching morphogenesis   (MGI Ref ID J:83430)
  • small kidney   (MGI Ref ID J:83430)
    • renal hypoplasia
      • kidney hypoplasia   (MGI Ref ID J:83430)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
      incomplete
Internal/Organ Defects
      kidney
Perinatal Lethality
      Homozygous

Internal/Organ Research
Kidney Defects

Research Tools
Internal/Organ Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Wnt11tm1Amc
Allele Name targeted mutation 1, Andrew P McMahon
Allele Type Targeted (Null/Knockout)
Common Name(s) Wnt11-;
Mutation Made By Andrew McMahon,   University of Southern California
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Wnt11, wingless-type MMTV integration site family, member 11
Chromosome 7
Gene Common Name(s) HWNT11;
Molecular Note The gene was disrupted by replacement of exons 4 and 5 with a PGK-neo cassette via homologous recombination. RT-PCR analysis of RNA from homozygous mutant animals revealed a truncated transcription product predicted to encode 28 N-terminal amino acids. This mutant protein is expected to be non-functional. [MGI Ref ID J:83430]

Genotyping

Genotyping Information

Genotyping Protocols

Wnt11tm1Amc, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Wnt11tm1Amc related

Andersson ER; Salto C; Villaescusa JC; Cajanek L; Yang S; Bryjova L; Nagy II; Vainio SJ; Ramirez C; Bryja V; Arenas E. 2013. Wnt5a cooperates with canonical Wnts to generate midbrain dopaminergic neurons in vivo and in stem cells. Proc Natl Acad Sci U S A 110(7):E602-10. [PubMed: 23324743]  [MGI Ref ID J:193686]

Chi L; Saarela U; Railo A; Prunskaite-Hyyrylainen R; Skovorodkin I; Anthony S; Katsu K; Liu Y; Shan J; Salgueiro AM; Belo JA; Davies J; Yokouchi Y; Vainio SJ. 2011. A secreted BMP antagonist, Cer1, fine tunes the spatial organization of the ureteric bud tree during mouse kidney development. PLoS One 6(11):e27676. [PubMed: 22114682]  [MGI Ref ID J:180958]

Cohen ED; Miller MF; Wang Z; Moon RT; Morrisey EE. 2012. Wnt5a and Wnt11 are essential for second heart field progenitor development. Development 139(11):1931-40. [PubMed: 22569553]  [MGI Ref ID J:184511]

Dawson K; Aflaki M; Nattel S. 2013. Role of the Wnt-Frizzled system in cardiac pathophysiology: a rapidly developing, poorly understood area with enormous potential. J Physiol 591(Pt 6):1409-32. [PubMed: 23207593]  [MGI Ref ID J:207360]

Majumdar A; Vainio S; Kispert A; McMahon J; McMahon AP. 2003. Wnt11 and Ret/Gdnf pathways cooperate in regulating ureteric branching during metanephric kidney development. Development 130(14):3175-85. [PubMed: 12783789]  [MGI Ref ID J:83430]

Nagy II; Railo A; Rapila R; Hast T; Sormunen R; Tavi P; Rasanen J; Vainio SJ. 2010. Wnt-11 signalling controls ventricular myocardium development by patterning N-cadherin and beta-catenin expression. Cardiovasc Res 85(1):100-9. [PubMed: 19622544]  [MGI Ref ID J:172560]

Ola R; Jakobson M; Kvist J; Perala N; Kuure S; Braunewell KH; Bridgewater D; Rosenblum ND; Chilov D; Immonen T; Sainio K; Sariola H. 2011. The GDNF target Vsnl1 marks the ureteric tip. J Am Soc Nephrol 22(2):274-84. [PubMed: 21289216]  [MGI Ref ID J:191040]

Stefater JA 3rd; Lewkowich I; Rao S; Mariggi G; Carpenter AC; Burr AR; Fan J; Ajima R; Molkentin JD; Williams BO; Wills-Karp M; Pollard JW; Yamaguchi T; Ferrara N; Gerhardt H; Lang RA. 2011. Regulation of angiogenesis by a non-canonical Wnt-Flt1 pathway in myeloid cells. Nature 474(7352):511-5. [PubMed: 21623369]  [MGI Ref ID J:173385]

Yu H; Ye X; Guo N; Nathans J. 2012. Frizzled 2 and frizzled 7 function redundantly in convergent extension and closure of the ventricular septum and palate: evidence for a network of interacting genes. Development 139(23):4383-94. [PubMed: 23095888]  [MGI Ref ID J:189062]

Zhou W; Lin L; Majumdar A; Li X; Zhang X; Liu W; Etheridge L; Shi Y; Martin J; Van de Ven W; Kaartinen V; Wynshaw-Boris A; McMahon AP; Rosenfeld MG; Evans SM. 2007. Modulation of morphogenesis by noncanonical Wnt signaling requires ATF/CREB family-mediated transcriptional activation of TGFbeta2. Nat Genet 39(10):1225-34. [PubMed: 17767158]  [MGI Ref ID J:125640]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to 129X1/SvJ (Stock No. 000691) inbred mice. Homozygotes exhibit some embryonic lethality and will die by 2 days post partem.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000691 129X1/SvJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.8)