Strain Name:

C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J

Stock Number:

006242

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These CCSP-rtTA transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat CCSP (or Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin)) gene promoter, and provide a "Tet-On" tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN5pN1
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice hemizygous for the CCSP-rtTA transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter. In situ hybridization detects rtTA gene product (mRNA) in bronchial and type II epithelial cells of lung tissue from adult transgenic mice treated with doxycycline for seven days. Induction of transgene expression is detected as early as postconception day 14 when the pregnant female is treated with doxycycline. When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. These CCSP-rtTA transgenic mice provide a "Tet-On" tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium.

Of note, these CCSP-rtTA mice are available on different genetic backgrounds including FVB/N (Stock No. 006222), C57BL/6J (Stock No. 006232), and BALB/cJ (Stock No. 006242).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A transgenic construct containing 3.7kb sequence of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and 2.0kb sequence of the human grouth hormone gene, containing introns and polyadenylation site, was injected into mixed C57BL/6 and 129 background embryos. Founder mice were bred to FVB/N animals. Upon arrival at The Jackson Laboratory, these mice were backcrossed to BALB/cJ mice for at least five generations.

Control Information

  Control
   Noncarrier
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Scgb1a1-rtTA)1Jaw allele
006232   B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
006222   FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
View Strains carrying   Tg(Scgb1a1-rtTA)1Jaw     (2 strains)

View Strains carrying other alleles of Scgb1a1     (4 strains)

View Strains carrying other alleles of rtTA     (20 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      lung

Immunology and Inflammation Research
Cystic Fibrosis

Internal/Organ Research
Lung Defects

Mouse/Human Gene Homologs
cystic fibrosis

Research Tools
Cancer Research
      Tetop Tet System
Developmental Biology Research
Genetics Research
      Mutagenesis and Transgenesis: Tetop Tet System
Immunology and Inflammation Research
Internal/Organ Research
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(Scgb1a1-rtTA)1Jaw
Allele Name transgene insertion 1, Jeffrey A Whitsett
Allele Type Transgenic (random, expressed)
Common Name(s) CC10-rtTA; CCSP-rtTA; CCSPtg; Tg(Ugb-rtTA)1Jaw;
Mutation Made By Jeffrey Whitsett,   Children's Hospital Medical Center
Strain of Origin129 and C57BL/6
Site of ExpressionExpresses rtTA in developing and adult lung.
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), rat
Molecular Note The transgene is composed of 2.3 kb of rat Ugb (Scgb1a1) promoter, a reverse tetracycline responsive transactivator gene (rt-TA), and 2 kb of human growth hormone sequences, which includes introns and a polyadenylation signal. Transgene expression was confirmed by RT-PCR analysis. The rat Scgb1a1 promoter is active in respiratory epithelial cells. [MGI Ref ID J:61736]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Scgb1a1-rtTA)1Jaw, Standard PCR
Tg(tTA), Melt Curve Analysis

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Tichelaar JW; Lu W; Whitsett JA. 2000. Conditional expression of fibroblast growth factor-7 in the developing and mature lung. J Biol Chem 275(16):11858-64. [PubMed: 10766812]  [MGI Ref ID J:61736]

Additional References

Tg(Scgb1a1-rtTA)1Jaw related

Akei H; Whitsett JA; Buroker M; Ninomiya T; Tatsumi H; Weaver TE; Ikegami M. 2006. Surface tension influences cell shape and phagocytosis in alveolar macrophages. Am J Physiol Lung Cell Mol Physiol 291(4):L572-9. [PubMed: 16632521]  [MGI Ref ID J:121210]

Akeson AL; Greenberg JM; Cameron JE; Thompson FY; Brooks SK; Wiginton D; Whitsett JA. 2003. Temporal and spatial regulation of VEGF-A controls vascular patterning in the embryonic lung. Dev Biol 264(2):443-55. [PubMed: 14651929]  [MGI Ref ID J:86840]

Borchers MT; Wesselkamper SC; Curull V; Ramirez-Sarmiento A; Sanchez-Font A; Garcia-Aymerich J; Coronell C; Lloreta J; Agusti AG; Gea J; Howington JA; Reed MF; Starnes SL; Harris NL; Vitucci M; Eppert BL; Motz GT; Fogel K; McGraw DW; Tichelaar JW; Orozco-Levi M. 2009. Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease. J Clin Invest 119(3):636-49. [PubMed: 19197141]  [MGI Ref ID J:146484]

Broide DH; Lawrence T; Doherty T; Cho JY; Miller M; McElwain K; McElwain S; Karin M. 2005. Allergen-induced peribronchial fibrosis and mucus production mediated by IkappaB kinase beta-dependent genes in airway epithelium. Proc Natl Acad Sci U S A 102(49):17723-8. [PubMed: 16317067]  [MGI Ref ID J:104395]

Bry K; Whitsett JA; Lappalainen U. 2007. IL-1beta disrupts postnatal lung morphogenesis in the mouse. Am J Respir Cell Mol Biol 36(1):32-42. [PubMed: 16888287]  [MGI Ref ID J:130523]

Chen SM; Cheng DS; Williams BJ; Sherrill TP; Han W; Chont M; Saint-Jean L; Christman JW; Sadikot RT; Yull FE; Blackwell TS. 2008. The nuclear factor kappa-B pathway in airway epithelium regulates neutrophil recruitment and host defence following Pseudomonas aeruginosa infection. Clin Exp Immunol 153(3):420-8. [PubMed: 18647324]  [MGI Ref ID J:139025]

Cheng DS; Han W; Chen SM; Sherrill TP; Chont M; Park GY; Sheller JR; Polosukhin VV; Christman JW; Yull FE; Blackwell TS. 2007. Airway epithelium controls lung inflammation and injury through the NF-kappa B pathway. J Immunol 178(10):6504-13. [PubMed: 17475880]  [MGI Ref ID J:146109]

Clark JC; Tichelaar JW; Wert SE; Itoh N; Perl AK; Stahlman MT; Whitsett JA. 2001. FGF-10 disrupts lung morphogenesis and causes pulmonary adenomas in vivo. Am J Physiol Lung Cell Mol Physiol 280(4):L705-15. [PubMed: 11238011]  [MGI Ref ID J:68525]

Dance-Barnes ST; Kock ND; Floyd HS; Moore JE; Mosley LJ; D'Agostino RB Jr; Pettenati MJ; Miller MS. 2008. Effects of mutant human Ki-ras(G12C) gene dosage on murine lung tumorigenesis and signaling to its downstream effectors. Toxicol Appl Pharmacol 231(1):77-84. [PubMed: 18565564]  [MGI Ref ID J:139881]

Dance-Barnes ST; Kock ND; Moore JE; Lin EY; Mosley LJ; D'Agostino RB Jr; McCoy TP; Townsend AJ; Miller MS. 2009. Lung tumor promotion by curcumin. Carcinogenesis 30(6):1016-23. [PubMed: 19359593]  [MGI Ref ID J:149324]

De Paepe ME; Gundavarapu S; Tantravahi U; Pepperell JR; Haley SA; Luks FI; Mao Q. 2008. Fas-ligand-induced apoptosis of respiratory epithelial cells causes disruption of postcanalicular alveolar development. Am J Pathol 173(1):42-56. [PubMed: 18535181]  [MGI Ref ID J:137382]

Duran A; Linares JF; Galvez AS; Wikenheiser K; Flores JM; Diaz-Meco MT; Moscat J. 2008. The signaling adaptor p62 is an important NF-kappaB mediator in tumorigenesis. Cancer Cell 13(4):343-54. [PubMed: 18394557]  [MGI Ref ID J:136133]

Engelman JA; Chen L; Tan X; Crosby K; Guimaraes AR; Upadhyay R; Maira M; McNamara K; Perera SA; Song Y; Chirieac LR; Kaur R; Lightbown A; Simendinger J; Li T; Padera RF; Garcia-Echeverria C; Weissleder R; Mahmood U; Cantley LC; Wong KK. 2008. Effective use of PI3K and MEK inhibitors to treat mutant Kras G12D and PIK3CA H1047R murine lung cancers. Nat Med 14(12):1351-6. [PubMed: 19029981]  [MGI Ref ID J:142254]

Fisher GH; Wellen SL; Klimstra D; Lenczowski JM; Tichelaar JW; Lizak MJ; Whitsett JA; Koretsky A; Varmus HE. 2001. Induction and apoptotic regression of lung adenocarcinomas by regulation of a K-Ras transgene in the presence and absence of tumor suppressor genes. Genes Dev 15(24):3249-62. [PubMed: 11751631]  [MGI Ref ID J:73468]

Floyd HS; Farnsworth CL; Kock ND; Mizesko MC; Little JL; Dance ST; Everitt J; Tichelaar J; Whitsett JA; Miller MS. 2005. Conditional expression of the mutant Ki-rasG12C allele results in formation of benign lung adenomas: development of a novel mouse lung tumor model. Carcinogenesis 26(12):2196-206. [PubMed: 16051643]  [MGI Ref ID J:102839]

Fulkerson PC; Fischetti CA; Hassman LM; Nikolaidis NM; Rothenberg ME. 2006. Persistent effects induced by IL-13 in the lung. Am J Respir Cell Mol Biol 35(3):337-46. [PubMed: 16645178]  [MGI Ref ID J:137329]

Galvez AS; Duran A; Linares JF; Pathrose P; Castilla EA; Abu-Baker S; Leitges M; Diaz-Meco MT; Moscat J. 2009. Protein kinase Czeta represses the interleukin-6 promoter and impairs tumorigenesis in vivo. Mol Cell Biol 29(1):104-15. [PubMed: 18955501]  [MGI Ref ID J:144073]

Glaros S; Cirrincione GM; Palanca A; Metzger D; Reisman D. 2008. Targeted knockout of BRG1 potentiates lung cancer development. Cancer Res 68(10):3689-96. [PubMed: 18483251]  [MGI Ref ID J:135031]

Hardie WD; Davidson C; Ikegami M; Leikauf GD; Le Cras TD; Prestridge A; Whitsett JA; Korfhagen TR. 2008. EGF receptor tyrosine kinase inhibitors diminish transforming growth factor-alpha-induced pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol 294(6):L1217-25. [PubMed: 18424623]  [MGI Ref ID J:136799]

Hardie WD; Korfhagen TR; Sartor MA; Prestridge A; Medvedovic M; Le Cras TD; Ikegami M; Wesselkamper SC; Davidson C; Dietsch M; Nichols W; Whitsett JA; Leikauf GD. 2007. Genomic profile of matrix and vasculature remodeling in TGF-alpha induced pulmonary fibrosis. Am J Respir Cell Mol Biol 37(3):309-21. [PubMed: 17496152]  [MGI Ref ID J:138490]

Ikegami M; Le Cras TD; Hardie WD; Stahlman MT; Whitsett JA; Korfhagen TR. 2005. TGF-alpha perturbs surfactant homeostasis in vivo. Am J Physiol Lung Cell Mol Physiol 289(1):L34-43. [PubMed: 15764643]  [MGI Ref ID J:101248]

Ikegami M; Whitsett JA; Martis PC; Weaver TE. 2005. Reversibility of lung inflammation caused by SP-B deficiency. Am J Physiol Lung Cell Mol Physiol 289(6):L962-70. [PubMed: 16024721]  [MGI Ref ID J:105014]

Ji H; Li D; Chen L; Shimamura T; Kobayashi S; McNamara K; Mahmood U; Mitchell A; Sun Y; Al-Hashem R; Chirieac LR; Padera R; Bronson RT; Kim W; Janne PA; Shapiro GI; Tenen D; Johnson BE; Weissleder R; Sharpless NE; Wong KK. 2006. The impact of human EGFR kinase domain mutations on lung tumorigenesis and in vivo sensitivity to EGFR-targeted therapies. Cancer Cell 9(6):485-95. [PubMed: 16730237]  [MGI Ref ID J:110131]

Ji H; Zhao X; Yuza Y; Shimamura T; Li D; Protopopov A; Jung BL; McNamara K; Xia H; Glatt KA; Thomas RK; Sasaki H; Horner JW; Eck M; Mitchell A; Sun Y; Al-Hashem R; Bronson RT; Rabindran SK; Discafani CM; Maher E; Shapiro GI; Meyerson M; Wong KK. 2006. Epidermal growth factor receptor variant III mutations in lung tumorigenesis and sensitivity to tyrosine kinase inhibitors. Proc Natl Acad Sci U S A 103(20):7817-22. [PubMed: 16672372]  [MGI Ref ID J:110098]

King NE; Zimmermann N; Pope SM; Fulkerson PC; Nikolaidis NM; Mishra A; Witte DP; Rothenberg ME. 2004. Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma. Am J Respir Cell Mol Biol 31(3):257-65. [PubMed: 15087305]  [MGI Ref ID J:101654]

Konstantinidou G; Bey EA; Rabellino A; Schuster K; Maira MS; Gazdar AF; Amici A; Boothman DA; Scaglioni PP. 2009. Dual phosphoinositide 3-kinase/mammalian target of rapamycin blockade is an effective radiosensitizing strategy for the treatment of non-small cell lung cancer harboring K-RAS mutations. Cancer Res 69(19):7644-52. [PubMed: 19789349]  [MGI Ref ID J:153586]

Kramer EL; Deutsch GH; Sartor MA; Hardie WD; Ikegami M; Korfhagen TR; Le Cras TD. 2007. Perinatal increases in TGF-{alpha} disrupt the saccular phase of lung morphogenesis and cause remodeling: microarray analysis. Am J Physiol Lung Cell Mol Physiol 293(2):L314-27. [PubMed: 17468132]  [MGI Ref ID J:125842]

Lange AW; Keiser AR; Wells JM; Zorn AM; Whitsett JA. 2009. Sox17 promotes cell cycle progression and inhibits TGF-beta/Smad3 signaling to initiate progenitor cell behavior in the respiratory epithelium. PLoS ONE 4(5):e5711. [PubMed: 19479035]  [MGI Ref ID J:148833]

Lappalainen U; Whitsett JA; Wert SE; Tichelaar JW; Bry K. 2005. Interleukin-1beta causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung. Am J Respir Cell Mol Biol 32(4):311-8. [PubMed: 15668323]  [MGI Ref ID J:107601]

Le Cras TD; Hardie WD; Deutsch GH; Albertine KH; Ikegami M; Whitsett JA; Korfhagen TR. 2004. Transient induction of TGF-alpha disrupts lung morphogenesis, causing pulmonary disease in adulthood. Am J Physiol Lung Cell Mol Physiol 287(4):L718-29. [PubMed: 15090366]  [MGI Ref ID J:96207]

Le Cras TD; Spitzmiller RE; Albertine KH; Greenberg JM; Whitsett JA; Akeson AL. 2004. VEGF causes pulmonary hemorrhage, hemosiderosis, and air space enlargement in neonatal mice. Am J Physiol Lung Cell Mol Physiol 287(1):L134-42. [PubMed: 15033636]  [MGI Ref ID J:99999]

Lian X; Qin Y; Hossain SA; Yang L; White A; Xu H; Shipley JM; Li T; Senior RM; Du H; Yan C. 2005. Overexpression of Stat3C in pulmonary epithelium protects against hyperoxic lung injury. J Immunol 174(11):7250-6. [PubMed: 15905571]  [MGI Ref ID J:98964]

Lin S; Ikegami M; Xu Y; Bosserhoff AK; Malkinson AM; Shannon JM. 2008. Misexpression of MIA disrupts lung morphogenesis and causes neonatal death. Dev Biol 316(2):441-55. [PubMed: 18342301]  [MGI Ref ID J:135990]

Lu TX; Munitz A; Rothenberg ME. 2009. MicroRNA-21 is up-regulated in allergic airway inflammation and regulates IL-12p35 expression. J Immunol 182(8):4994-5002. [PubMed: 19342679]  [MGI Ref ID J:147494]

Mason-Richie NA; Mistry MJ; Gettler CA; Elayyadi A; Wikenheiser-Brokamp KA. 2008. Retinoblastoma function is essential for establishing lung epithelial quiescence after injury. Cancer Res 68(11):4068-76. [PubMed: 18519665]  [MGI Ref ID J:136426]

Mucenski ML; Nation JM; Thitoff AR; Besnard V; Xu Y; Wert SE; Harada N; Taketo MM; Stahlman MT; Whitsett JA. 2005. Beta-catenin regulates differentiation of respiratory epithelial cells in vivo. Am J Physiol Lung Cell Mol Physiol 289(6):L971-9. [PubMed: 16040629]  [MGI Ref ID J:105013]

Mucenski ML; Wert SE; Nation JM; Loudy DE; Huelsken J; Birchmeier W; Morrisey EE; Whitsett JA. 2003. beta-Catenin is required for specification of proximal/distal cell fate during lung morphogenesis. J Biol Chem 278(41):40231-8. [PubMed: 12885771]  [MGI Ref ID J:85946]

Nesslein LL; Melton KR; Ikegami M; Na CL; Wert SE; Rice WR; Whitsett JA; Weaver TE. 2005. Partial SP-B deficiency perturbs lung function and causes air space abnormalities. Am J Physiol Lung Cell Mol Physiol 288(6):L1154-61. [PubMed: 15722377]  [MGI Ref ID J:101247]

Park KS; Wells JM; Zorn AM; Wert SE; Whitsett JA. 2006. Sox17 influences the differentiation of respiratory epithelial cells. Dev Biol 294(1):192-202. [PubMed: 16574095]  [MGI Ref ID J:109303]

Perera SA; Li D; Shimamura T; Raso MG; Ji H; Chen L; Borgman CL; Zaghlul S; Brandstetter KA; Kubo S; Takahashi M; Chirieac LR; Padera RF; Bronson RT; Shapiro GI; Greulich H; Meyerson M; Guertler U; Chesa PG; Solca F; Wistuba II; Wong KK. 2009. HER2YVMA drives rapid development of adenosquamous lung tumors in mice that are sensitive to BIBW2992 and rapamycin combination therapy. Proc Natl Acad Sci U S A 106(2):474-9. [PubMed: 19122144]  [MGI Ref ID J:143874]

Perl AK; Tichelaar JW; Whitsett JA. 2002. Conditional gene expression in the respiratory epithelium of the mouse. Transgenic Res 11(1):21-9. [PubMed: 11874100]  [MGI Ref ID J:129109]

Perl AK; Wert SE; Loudy DE; Shan Z; Blair PA; Whitsett JA. 2005. Conditional recombination reveals distinct subsets of epithelial cells in trachea, bronchi, and alveoli. Am J Respir Cell Mol Biol 33(5):455-62. [PubMed: 16055670]  [MGI Ref ID J:132772]

Politi K; Zakowski MF; Fan PD; Schonfeld EA; Pao W; Varmus HE. 2006. Lung adenocarcinomas induced in mice by mutant EGF receptors found in human lung cancers respond to a tyrosine kinase inhibitor or to down-regulation of the receptors. Genes Dev 20(11):1496-510. [PubMed: 16705038]  [MGI Ref ID J:109092]

Qu P; Du H; Wang X; Yan C. 2009. Matrix metalloproteinase 12 overexpression in lung epithelial cells plays a key role in emphysema to lung bronchioalveolar adenocarcinoma transition. Cancer Res 69(18):7252-61. [PubMed: 19706765]  [MGI Ref ID J:152404]

Regales L; Balak MN; Gong Y; Politi K; Sawai A; Le C; Koutcher JA; Solit DB; Rosen N; Zakowski MF; Pao W. 2007. Development of new mouse lung tumor models expressing EGFR T790M mutants associated with clinical resistance to kinase inhibitors. PLoS ONE 2(8):e810. [PubMed: 17726540]  [MGI Ref ID J:128700]

Scaglioni PP; Yung TM; Cai LF; Erdjument-Bromage H; Kaufman AJ; Singh B; Teruya-Feldstein J; Tempst P; Pandolfi PP. 2006. A CK2-dependent mechanism for degradation of the PML tumor suppressor. Cell 126(2):269-83. [PubMed: 16873060]  [MGI Ref ID J:144680]

Sisson TH; Hanson KE; Subbotina N; Patwardhan A; Hattori N; Simon RH. 2002. Inducible lung-specific urokinase expression reduces fibrosis and mortality after lung injury in mice. Am J Physiol Lung Cell Mol Physiol 283(5):L1023-32. [PubMed: 12376355]  [MGI Ref ID J:80184]

Tomita T; Kido T; Kurotani R; Iemura S; Sterneck E; Natsume T; Vinson C; Kimura S. 2008. CAATT/enhancer-binding proteins alpha and delta interact with NKX2-1 to synergistically activate mouse secretoglobin 3A2 gene expression. J Biol Chem 283(37):25617-27. [PubMed: 18632661]  [MGI Ref ID J:141842]

Tran TP; Fan AC; Bendapudi PK; Koh S; Komatsubara K; Chen J; Horng G; Bellovin DI; Giuriato S; Wang CS; Whitsett JA; Felsher DW. 2008. Combined Inactivation of MYC and K-Ras oncogenes reverses tumorigenesis in lung adenocarcinomas and lymphomas. PLoS ONE 3(5):e2125. [PubMed: 18461184]  [MGI Ref ID J:136212]

Wan H; Kaestner KH; Ang SL; Ikegami M; Finkelman FD; Stahlman MT; Fulkerson PC; Rothenberg ME; Whitsett JA. 2004. Foxa2 regulates alveolarization and goblet cell hyperplasia. Development 131(4):953-64. [PubMed: 14757645]  [MGI Ref ID J:88601]

Weng Y; Fang C; Turesky RJ; Behr M; Kaminsky LS; Ding X. 2007. Determination of the role of target tissue metabolism in lung carcinogenesis using conditional cytochrome P450 reductase-null mice. Cancer Res 67(16):7825-32. [PubMed: 17699788]  [MGI Ref ID J:124316]

Wesselkamper SC; Eppert BL; Motz GT; Lau GW; Hassett DJ; Borchers MT. 2008. NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infection. J Immunol 181(8):5481-9. [PubMed: 18832705]  [MGI Ref ID J:140762]

Zhou X; Bao H; Al-Hashem R; Ji H; Albert M; Wong KK; Sun Y. 2008. Magnetic resonance imaging of the response of a mouse model of non-small cell lung cancer to tyrosine kinase inhibitor treatment. Comp Med 58(3):276-81. [PubMed: 18589870]  [MGI Ref ID J:140430]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as hemizygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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