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Strain Name:

C.Cg-Tg(SFTPC-rtTA)5Jaw/J

Stock Number:

006245

Availability:

Repository- Live

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Genes & Alleles   SFTPC;   rtTA;   Tg(SFTPC-rtTA)5Jaw;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator IMR Colony,   The Jackson Laboratory
GenerationN5F1 (12-MAY-08)

Strain Description
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. rtTA activity is detected in lung peripheral epithelial cells from adult mice and in embryos from pregnant females treated with the tetracycline analog, doxycycline (dox). In the latter, rtTA-induced expression of a luciferase reporter under the regulation of a tetracycline-responsive promoter (TRE; tetO) has been detected as early as embryonic day 10.5. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a TRE, expression of the target gene in the bitransgenic offspring may be regulated by dox; in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a "Tet-On" tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium. Please note that some level of rtTA expression may be present in lung tissue from mice untreated with dox.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles frequently are moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Strain Development
A transgenic construct containing 3.7kb sequence of the human SFTPC, surfactant, pulmonary-associated protein C, gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and SV40 polyadenylation signal sequence was injected into mixed C57BL/6 and 129 background embryos. Founder mice were bred to FVB/N animals. Upon arrival at The Jackson Laboratory, these mice were backcrossed to BALB/cJ mice for at least 5 generations

Gene & Allele Details

Allele Symbol Tg(SFTPC-rtTA)5Jaw
Allele Name transgene insertion 5, Jeffrey A Whitsett
Common Name(s) SP-C-rtTAtg; SP-CrtTA; SPC-rtTA; Sp-c-rtta; Tg(SFTPC-rtTA)1Jaw; hSPC-rtTA+;
Mutation Made By Jeffrey Whitsett,   Children's Hospital Medical Center
Site of ExpressionExpresses rtTA in developing and adult lung.
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter SFTPC, surfactant, pulmonary-associated protein C, human
Molecular Note The transgene is composed of 3.7 kb of human SFTPC promoter, a reverse tetracycline responsive transactivator gene (rt-TA), and a 0.45 kb SV40 polyadenylation signal. Transgene expression was confirmed by RT-PCR analysis. The human SFTPC promoter is active in respiratory epithelial cells. [MGI Ref ID J:61736]

Control Information

  Control
   Noncarrier
   000651 BALB/cJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(SFTPC-rtTA)5Jaw

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as hemizygotes.

Related Strains

Strains carrying   Tg(SFTPC-rtTA)5Jaw allele
006235   B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006225   FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
View Strains carrying   Tg(SFTPC-rtTA)5Jaw     (2 strains)

View Strains carrying other alleles of rtTA     (15 strains)

Additional Web Information

Congenic Nomenclature
Tet Expression Systems

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects (lung)

Immunology and Inflammation Research
Cystic Fibrosis

Internal/Organ Research
Lung Defects

Mouse/Human Gene Homologs
cystic fibrosis

Research Tools
Cancer Research (Tetop Tet System)
Developmental Biology Research
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Immunology and Inflammation Research
Internal/Organ Research
Tet Expression Systems (tTA/rtTA Expressing Strains)

References

Selected Reference(s)

Tichelaar JW; Lu W; Whitsett JA. 2000. Conditional expression of fibroblast growth factor-7 in the developing and mature lung. J Biol Chem 275(16):11858-64. [PubMed: 10766812]  [MGI Ref ID J:61736]

Additional References

Price and Supply Information

Strain Name: C.Cg-Tg(SFTPC-rtTA)5Jaw/J
Stock Number: 006245

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

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- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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