Strain Name: |
C.Cg-Tg(SFTPC-rtTA)5Jaw/J |
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Stock Number: |
006245 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | SFTPC; rtTA; Tg(SFTPC-rtTA)5Jaw; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N5F1 (12-MAY-08) Strain Description
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. rtTA activity is detected in lung peripheral epithelial cells from adult mice and in embryos from pregnant females treated with the tetracycline analog, doxycycline (dox). In the latter, rtTA-induced expression of a luciferase reporter under the regulation of a tetracycline-responsive promoter (TRE; tetO) has been detected as early as embryonic day 10.5. When hemizygotes are mated to a second transgenic strain carrying a gene of interest under the regulatory control of a TRE, expression of the target gene in the bitransgenic offspring may be regulated by dox; in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a "Tet-On" tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium. Please note that some level of rtTA expression may be present in lung tissue from mice untreated with dox.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles frequently are moved to a genetic background different from that on which an allele was first characterized. This is the case for this strain. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing 3.7kb sequence of the human SFTPC, surfactant, pulmonary-associated protein C, gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and SV40 polyadenylation signal sequence was injected into mixed C57BL/6 and 129 background embryos. Founder mice were bred to FVB/N animals. Upon arrival at The Jackson Laboratory, these mice were backcrossed to BALB/cJ mice for at least 5 generations
| Allele Symbol | Tg(SFTPC-rtTA)5Jaw | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Jeffrey A Whitsett | ||
| Common Name(s) | SP-C-rtTAtg; SP-CrtTA; SPC-rtTA; Sp-c-rtta; Tg(SFTPC-rtTA)1Jaw; hSPC-rtTA+; | ||
| Mutation Made By | Jeffrey Whitsett, Children's Hospital Medical Center | ||
| Site of Expression | Expresses rtTA in developing and adult lung. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | SFTPC, surfactant, pulmonary-associated protein C, human | ||
| Molecular Note | The transgene is composed of 3.7 kb of human SFTPC promoter, a reverse tetracycline responsive transactivator gene (rt-TA), and a 0.45 kb SV40 polyadenylation signal. Transgene expression was confirmed by RT-PCR analysis. The human SFTPC promoter is active in respiratory epithelial cells. [MGI Ref ID J:61736] | ||
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Tg(SFTPC-rtTA)5Jaw
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
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Strains carrying Tg(SFTPC-rtTA)5Jaw allele
006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J View Strains carrying Tg(SFTPC-rtTA)5Jaw (2 strains)
Strains carrying other alleles of rtTA
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J View Strains carrying other alleles of rtTA (15 strains)
Congenic Nomenclature
Tet Expression Systems
Room Number AX11
Developmental Biology Research
Internal/Organ Defects (lung)
Immunology and Inflammation Research
Cystic Fibrosis
Internal/Organ Research
Lung Defects
Mouse/Human Gene Homologs
cystic fibrosis
Research Tools
Cancer Research (Tetop Tet System)
Developmental Biology Research
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Immunology and Inflammation Research
Internal/Organ Research
Tet Expression Systems (tTA/rtTA Expressing Strains)
Selected Reference(s)
Additional ReferencesTichelaar JW; Lu W; Whitsett JA. 2000. Conditional expression of fibroblast growth factor-7 in the developing and mature lung. J Biol Chem 275(16):11858-64. [PubMed: 10766812] [MGI Ref ID J:61736]
| Strain Name: | C.Cg-Tg(SFTPC-rtTA)5Jaw/J |
| Stock Number: | 006245 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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