Strain Name: |
NOD.Cg-Tg(Ins2-Ccl21b)2Cys/JbsJ |
|---|---|
Stock Number: |
006254 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Strain Common Names | NOD.CCL21; NOD.RIP-SLC; NOD.SLC; |
| Genes & Alleles | Ccl21b; Ins2; Tg(Ins2-Ccl21b)2Cys; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain B6D2F1/J Donating Investigator Jeffrey Bluestone, University of California, San Francisco H2 Haplotype g7 Generation N12+2pN1 Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
Ins2-Ccl21-leu (commonly referred to as SLC) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Immunohistochemistry of pancreatic sections with antibodies to CCL21 demonstrate detectable chemokine protein within the pancreatic beta cells. In transgenic mice on the B6.D2 background, SLC pancreatic expression leads to highly organized infiltrates in the pancreatic islets. These infiltrates contain B cells, CD4+ and CD8+ T cells and dendritic cells (DCs) with the DCs and and T cells localized close to the CCL21b expressing islet cells.According to the donating investigator, SLC expression in the pancrease of transgenic mice on the NOD congenic background prevents diabetes development (unpublished communication).
This strain is useful for the study of T cells, B cells and chemokines in the development of diabetes.
Strain Development
A transgenic construct encoding the mouse Ccl21-leu (Ccl21b) cDNA fused to rat insulin promoter 7 (Ins2) was injected into B6D2F1 fertilized embryos. Approximately 4 copies of the Ins2-Ccl21 transgene integrated into the genome. Progeny of founder 4 were mated to C57BL/6 for several generations prior to mating to NOD for 12 generations. In 2006, the T1DR received transgenic mice at generation N12 and crossed them twice to NOD/ShiLtJ (Stock No. 001976) prior to sibling mating.
Gene & Allele Details
| Allele Symbol | Tg(Ins2-Ccl21b)2Cys | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Jason G Cyster | ||
| Common Name(s) | RIP-CCL21; RIP-SLC; | ||
| Mutation Made By | Jason Cyster, University of California San Francisco | ||
| Strain of Origin | (C57BL/6 x DBA/2)F1 | ||
| Expressed Gene | Ccl21b, chemokine (C-C motif) ligand 21b, mouse, laboratory | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | A full-length cDNA of murine Ccl21b was inserted into a construct containing the promoter/enhancer intron region (RIP7) of the rat insulin promoter 2. The resulting transgenic line II (2) contains 4 copies of the transgene and was used for further analyses. [MGI Ref ID J:77238] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-Ccl21b)2Cys
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Lymphoid Tissue Defects (Lymphocyte Homing)
References
Selected Reference(s)
Luther SA; Bidgol A; Hargreaves DC; Schmidt A; Xu Y; Paniyadi J; Matloubian M; Cyster JG. 2002. Differing activities of homeostatic chemokines CCL19, CCL21, and CXCL12 in lymphocyte and dendritic cell recruitment and lymphoid neogenesis. J Immunol 169(1):424-33. [PubMed: 12077273] [MGI Ref ID J:77238]
Price and Supply Information
| Strain Name: | NOD.Cg-Tg(Ins2-Ccl21b)2Cys/JbsJ |
| Stock Number: | 006254 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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