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Strain Name:

NOD.Cg-Tg(Ins2-Ccl21b)2Cys/JbsJ

Stock Number:

006254

Availability:

Repository-Cryopreserved


General Terms and Conditions

Strain Common Names      NOD.CCL21;      NOD.RIP-SLC;      NOD.SLC;
Genes & Alleles   Ccl21b;   Ins2;   Tg(Ins2-Ccl21b)2Cys;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating System+/+ sibling x Hemizygote         (Female x Male)
Specieslaboratory mouse
Background Strain NOD/ShiLtJ
Donor Strain B6D2F1/J
Donating Investigator Jeffrey Bluestone,   University of California, San Francisco
H2 Haplotypeg7
GenerationN12+2pN1

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Strain Description
Ins2-Ccl21-leu (commonly referred to as SLC) transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Immunohistochemistry of pancreatic sections with antibodies to CCL21 demonstrate detectable chemokine protein within the pancreatic beta cells. In transgenic mice on the B6.D2 background, SLC pancreatic expression leads to highly organized infiltrates in the pancreatic islets. These infiltrates contain B cells, CD4+ and CD8+ T cells and dendritic cells (DCs) with the DCs and and T cells localized close to the CCL21b expressing islet cells.

According to the donating investigator, SLC expression in the pancrease of transgenic mice on the NOD congenic background prevents diabetes development (unpublished communication).

This strain is useful for the study of T cells, B cells and chemokines in the development of diabetes.

Strain Development
A transgenic construct encoding the mouse Ccl21-leu (Ccl21b) cDNA fused to rat insulin promoter 7 (Ins2) was injected into B6D2F1 fertilized embryos. Approximately 4 copies of the Ins2-Ccl21 transgene integrated into the genome. Progeny of founder 4 were mated to C57BL/6 for several generations prior to mating to NOD for 12 generations. In 2006, the T1DR received transgenic mice at generation N12 and crossed them twice to NOD/ShiLtJ (Stock No. 001976) prior to sibling mating.

Gene & Allele Details

Allele Symbol Tg(Ins2-Ccl21b)2Cys
Allele Name transgene insertion 2, Jason G Cyster
Common Name(s) RIP-CCL21; RIP-SLC;
Mutation Made By Jason Cyster,   University of California San Francisco
Strain of Origin(C57BL/6 x DBA/2)F1
Expressed Gene Ccl21b, chemokine (C-C motif) ligand 21b, mouse, laboratory
Promoter Ins2, insulin 2, rat
Molecular Note A full-length cDNA of murine Ccl21b was inserted into a construct containing the promoter/enhancer intron region (RIP7) of the rat insulin promoter 2. The resulting transgenic line II (2) contains 4 copies of the transgene and was used for further analyses. [MGI Ref ID J:77238]

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(Ins2-Ccl21b)2Cys

Related Strains

Strains carrying other alleles of Ins2
005534   B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ
005500   B6.C-Tg(Ins2-GP)34-20Olds/MvhJ
005715   B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ
004826   B6.Cg-Tg(Ins2-NP)25-3Olds/MhvJ
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
005713   C.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ
005533   C.Cg-Tg(Ins2-HA)165Bri/ShrmJ
004827   C.Cg-Tg(Ins2-NP)25-3Olds/MvhJ
005432   C57BL/6-Tg(Ins2-OVA)307Wehi/WehiJ
005433   C57BL/6-Tg(Ins2-OVA)59Wehi/WehiJ
005431   C57BL/6-Tg(Ins2-TFRC/OVA)296Wehi/WehiJ
005564   FVB(Cg)-Tg(Ins2-CALM1)26Ove Tg(Cryaa-TAg)1Ove/PneJ
008232   FVB/N-Tg(Ins2-IAPP)RHFSoel/J
005522   NOD-Tg(Ins2*Y16A)1Ell/GseJ
005523   NOD-Tg(Ins2*Y16A)3Ell/GseJ
003499   NOD-Tg(Ins2-Fasl)24Ach
007840   NOD.Cg-Prkdcscid Tg(Ins2-CD86)12B70Flv/FswJ
004346   NOD.Cg-Prkdcscid Tg(Ins2-CD80)3B7Flv/DvsJ
004230   NOD.Cg-Prkdcscid Tg(Ins2-E3)1Dvs/DvsJ
003843   NOD.Cg-Prkdcscid Tg(Ins2-GAD2)1Lt/LtJ
003844   NOD.Cg-Prkdcscid Tg(Ins2-GAD2)2Lt/LtJ
005524   NOD.Cg-Tg(Ins2*Y16A)1Ell Ins1tm1Jja Ins2tm1Jja/GseJ
005525   NOD.Cg-Tg(Ins2*Y16A)3Ell Ins1tm1Jja Ins2tm1Jja/GseJ
006154   NOD.Cg-Tg(Ins2-Cxcl13)1Cys/JbsJ
003869   NOD.Cg-Tg(Ins2-E3)1Dvs/DvsJ
005685   NOD.Cg-Tg(Ins2-HA)165Bri/ShrmJ
002380   NOD.Cg-Tg(Ins2-TAg)1Lt Prkdcscid/DvsJ
004602   NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ
004937   NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ
005734   NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
005870   NOD/ShiLt(Cg)-Tg(Ins2-GAD2)2Lt/J
006777   NOD/ShiLt-Tg(Ins2-Cd274)2Mdos/MdosJ
005733   NOD/ShiLt-Tg(Ins2-Fas*I246N)1Ach/AchJ
003074   NOD/ShiLt-Tg(Ins2-GAD2)1Lt/LtJ
004986   NOD/ShiLt-Tg(Ins2-cre)3Lt/Lt
003855   NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
004987   NOD/ShiLt-Tg(Ins2-cre)6Lt/Lt
002033   NOD/ShiLt-Tg(RipTAg)1Lt/J
004990   NOD/ShiLtDvs-Tg(Ins2-E3*734)4Dvs/DvsJ
005714   NOR.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ
008122   STOCK Tg(Ins2-cre/Esr1)1Dam/J
008250   STOCK Tg(Ins2-rtTA)2Efr/J
View Strains carrying other alleles of Ins2     (42 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)

Immunology and Inflammation Research
Lymphoid Tissue Defects (Lymphocyte Homing)

References

Selected Reference(s)

Luther SA; Bidgol A; Hargreaves DC; Schmidt A; Xu Y; Paniyadi J; Matloubian M; Cyster JG. 2002. Differing activities of homeostatic chemokines CCL19, CCL21, and CXCL12 in lymphocyte and dendritic cell recruitment and lymphoid neogenesis. J Immunol 169(1):424-33. [PubMed: 12077273]  [MGI Ref ID J:77238]


Price and Supply Information

Strain Name: NOD.Cg-Tg(Ins2-Ccl21b)2Cys/JbsJ
Stock Number: 006254

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Type 1 Diabetes Repository collection.

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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