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Genes & Alleles

Pepc;   Ptprc;   UBC;   Tg(UBC-scFv)2Nemz;   ;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator David Nemazee,   The Scripps Research Institute Generation ?+F1 (17-JUL-08)

Strain Description
"Kappa-macroself Ag#2" transgenic mice express a membrane-bound "macroself" superantigen with specificity for the mouse immunoglobulin kappa light chain (Igk). Hemizygous mice are viable and fertile. The transgene is expressed in virtually all cells tested, including lymphoid tissue and bone marrow. B cells expressing the Igk-macroself superantigen are absent from the peripheral lymphoid organs while Ig l⁺ B cell numbers are substantially increased. Although bone marrow B cell numbers are unchanged, total peripheral B cell populations are reduced by approximately one half. These mice also express the CD45.1 (Ly5.1 or Ptprca) allele, which is atypical for this C57BL/6 (B6) congenic background, and this marker may be used to track donor cell populations in bone marrow transplantation studies with B6 (CD45.2: Ptprc^b) mice. These "kappa-macroself Ag#2" transgenic mice may be useful to study B cell receptor editing and B cell tolerance in a polyclonal immune system, and to rapidly screen other mutations for potential defects in B cell tolerance.

Genotyping Note: As the transgene expresses a superantigen reactive to immunoglobulin kappa of mouse and may interfere with antibody specificity if using mouse anti-CD45 antibodies, a pre-blocking step using unlabeled mouse IgG:kappa (or equivalent blocking step) is recommended for FACS-typing of the Ptprc (CD45) allele.

Strain Development
These mice are designed to express a membrane-tethered single-chain variable fragment antibody with specificity to mouse Igk light chain. The 4 kb Ulik transgene contains the 1.2 kb human ubiquitin C promoter, rheumatoid factor Ig light chain gene leader exon and first intron, the expressed variable genes from the rat anti-mouse Ig Ck hybridoma 187 line, the hinge, CH2, and CH3 sequences from rat IgG1 heavy chain cDNA , and the transmembrane and intracytoplasmic sequences from the mouse H2-k^b gene. This construct was microinjected into (B6xDBA/2)F1 embryos and then implanted into pseudopregnant Igk-deficient foster dams. "Kappa-macroself" transgenic mice (founder line 2) were backcrossed to mice on a "B6.CD45.1" background (derived from Stock No. 002014, B6.SJL-Ptprc^a Pepc^b/BoyJ) for approximately 20 generations before arriving at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol		Tg(UBC-scFv)2Nemz
Allele Name		transgene insertion 2, David Nemazee
Common Name(s)		Ulik; kappa-macroself;
Mutation Made By		Djemel Ait-Azzouzene,   The Scripps Research Institute
Strain of Origin		(C57BL/6 x DBA/2)F1
Promoter		UBC, ubiquitin C, human
Molecular Note		These mice are designed to express a membrane-tethered single chain variable fragment antibody with specificity to mouse Igk light chain. The 4 kb "Ulik" transgene contained the 1.2 kb human ubiquitin C promoter, rheumatoid factor Ig light chain gene leader exon and first intron, the expressed variable genes from the rat anti-k hybridoma 187 line, the hinge, CH2, and CH3 sequences from rat gamma chain 1 (CH1 domain deleted), and the transmembrane and intracytoplasmic sequences from the H2-kb gene. [MGI Ref ID J:96759]
Gene Symbol and Name		Pepc, peptidase C
Chromosome		1
Gene Common Name(s)		Dip-1; Pep-3; Pep3; dipeptidase 1; peptidase 3;
Gene Symbol and Name		Ptprc, protein tyrosine phosphatase, receptor type, C
Chromosome		1
Gene Common Name(s)		B220; CD45; CD45 antigen; CD45R; Cd45; GP180; L-CA; LCA; LY5; Ly-5; Lyt-4; RT7; T-lymphocyte antigen 4; T200; lymphocyte antigen 5;

Control Information

	Control
	Noncarrier
	002014 B6.SJL-Ptprca Pepcb/BoyJ	(approximate)
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Tg(UBC-scFv)2Nemz

Colony Maintenance

Breeding & Husbandry

When maintaining a live colony, hemizygous mice can be bred to wildtype siblings or to Stock No. 002014. The donating investigator has not attempted to breed hemizygous mice together. Genotyping Note: As the transgene expresses a superantigen reactive to immunoglobulin kappa of mouse and may interfere with antibody specificity if using mouse anti-CD45 antibodies, a pre-blocking step using unlabeled mouse IgG:kappa (or equivalent blocking step) is recommended for FACS-typing of the Ptprc (CD45) allele.

Diet Information

LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Autoimmunity (B cell deficiency)
Immunodeficiency (B cell deficiency)
Immunodeficiency (defects in humoral immune responses)
Lymphoid Tissue Defects

Research Tools
Immunology and Inflammation Research (B cell deficiency)
Immunology and Inflammation Research (production of B cells and antibodies)

References

Selected Reference(s)

Ait-Azzouzene D; Verkoczy L; Peters J; Gavin A; Skog P; Vela JL; Nemazee D. 2005. An immunoglobulin C{kappa}-reactive single chain antibody fusion protein induces tolerance through receptor editing in a normal polyclonal immune system. J Exp Med 201(5):817-28. [PubMed: 15738053]  [MGI Ref ID J:96759]

Additional References

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Price and Supply Information

Strain Name:	B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J
Stock Number:	006259

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date: 01-SEP-08

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

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Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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