| |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.129P2-Fut2tm1Sdo/J (Changed: 27-AUG-08 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N14+F4 (08-JAN-09) Donating Investigator Steven Domino, University of Michigan Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have a chinchilla (gray) coat color, while heterozygotes have the typical black coat color expected for the C57BL/6J genetic background. Alpha (1,2) fucosylated glycans are not detected in the uterine epithelia from homozygotes at estrus. Beta galactosidase staining is detected in endocervial and uterine gland mucus secreting cells, stomach foveolar pit cells and chief cells, and colon goblet cells. The pattern of beta galactosidase activity mimics the endogenous expression pattern of the endogenous gene. This mutant mouse strain represents a model of the nonsecretor ABH histo-blood group antigen, which confers resistance to Norwalk virus infection, and may be useful in studies of reproductive biology, gastrointestinal tract epithelium, and the function of fucosylated glycans.
This strain was transferred from the collection of the Consortium for Functional Glycomics.Development
A targeting vector containing neomycin resistance and beta-galactosidase genes was used to disrupt the exon encoding the catalytic domain. The construct was electroporated into 129X1/SvJ derived Pat5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were crossed to C57BL6J females. Heterozygotes were intercrossed to generate homozygotes. The mice were then backcrossed to C57BL/6J for 13 generations
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (183 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Fut2tm1Sdo/Fut2tm1Sdo
B6.129X1-Fut2tm1Sdo
- reproductive system phenotype
- abnormal female reproductive system physiology (MGI Ref ID J:90993)
- fucosylated glycans absent in the endocervix and upper vagina
- immune system phenotype
- increased susceptibility to fungal infection (MGI Ref ID J:90993)
- increased susceptibility to vaginal infection with Candida albicans
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Fut2tm1Sdo/Fut2tm1Sdo
involves: 129X1/SvJ * C57BL/6J
- reproductive system phenotype
- abnormal female reproductive system physiology (MGI Ref ID J:89929)
- fucosylated glycolipids absent from female reproductive organs
- abnormal uterine environment (MGI Ref ID J:72946)
- absence of fucosylated glycans in the uterine epithelia but normal female fertility
- digestive/alimentary phenotype
- abnormal digestive system physiology (MGI Ref ID J:89929)
- fucosylated glycolipids absent from the colon
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Fut2tm1Sdo | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Steven E Domino | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | FUT2-; Fut2-LacZ; | ||
| Mutation Made By | Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | Pat5 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | lacZ expression patterns have not been characterized. | ||
| Gene Symbol and Name | Fut2, fucosyltransferase 2 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | B12QTL1; Ftc; Futb; SE; SEC2; Se2; sej; | ||
| General Note | The embryonic stem cell lines Pat5 and R1 were used for gene targeting. | ||
| Molecular Note | Most of the single coding exon of the gene was replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the catalytic domain. Homozygous mutant animals were identified by Southern blot and PCR genotype analysis. [MGI Ref ID J:72946] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fut2tm1Sdo, MCA, vers. 2
Fut2tm1Sdo, STD PCR, vers. 1
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Domino SE; Zhang L; Gillespie PJ; Saunders TL; Lowe JB. 2001. Deficiency of Reproductive Tract alpha(1,2)Fucosylated Glycans and Normal Fertility in Mice with Targeted Deletions of the FUT1 or FUT2 alpha(1,2)Fucosyltransferase Locus. Mol Cell Biol 21(24):8336-45. [PubMed: 11713270] [MGI Ref ID J:72946]
Additional References
Fut2tm1Sdo relatedDomino SE; Hurd EA. 2004. LacZ expression in Fut2-LacZ reporter mice reveals estrogen-regulated endocervical glandular expression during estrous cycle, hormone replacement, and pregnancy. Glycobiology 14(2):169-75. [PubMed: 14576173] [MGI Ref ID J:105335]
Domino SE; Karnak DM; Hurd EA. 2007. Cell surface fucosylation does not affect development of colon tumors in mice with germline Smad3 mutation. Tumour Biol 28(2):77-83. [PubMed: 17264540] [MGI Ref ID J:121796]
Hurd EA; Domino SE. 2004. Increased susceptibility of secretor factor gene Fut2-null mice to experimental vaginal candidiasis. Infect Immun 72(7):4279-81. [PubMed: 15213174] [MGI Ref ID J:90993]
Iwamori M; Domino SE. 2004. Tissue-specific loss of fucosylated glycolipids in mice with targeted deletion of alpha(1,2)fucosyltransferase genes. Biochem J 380(Pt 1):75-81. [PubMed: 14967068] [MGI Ref ID J:89929]
St John JA; Claxton C; Robinson MW; Yamamoto F; Domino SE; Key B. 2006. Genetic manipulation of blood group carbohydrates alters development and pathfinding of primary sensory axons of the olfactory systems. Dev Biol 298(2):470-84. [PubMed: 16884711] [MGI Ref ID J:119285]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Of note, homozygous mice have a chinchilla (gray) coat color, while heterozygotes have the typical black coat color expected for the C57BL/6J genetic background. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $171.30 Female or Male Homozygous for Fut2tm1Sdo
Pairs /Price (US dollars $) Pair Genotype $342.60 Homozygous for Fut2tm1Sdo x Homozygous for Fut2tm1Sdo
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $222.70 Female or Male Homozygous for Fut2tm1Sdo
Pairs /Price (US dollars $) Pair Genotype $445.40 Homozygous for Fut2tm1Sdo x Homozygous for Fut2tm1Sdo
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.