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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Former Names B6.129P2-Fut2tm1Sdo/J (Changed: 27-AUG-08 ) Type Congenic; Mutant Strain; Targeted Mutation; Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N14+F2 (20-DEC-07) Donating Investigator Steven Domino, University of Michigan Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have a chinchilla (gray) coat color, while heterozygotes have the typical black coat color expected for the C57BL/6J genetic background. Alpha (1,2) fucosylated glycans are not detected in the uterine epithelia from homozygotes at estrus. Beta galactosidase staining is detected in endocervial and uterine gland mucus secreting cells, stomach foveolar pit cells and chief cells, and colon goblet cells. The pattern of beta galactosidase activity mimics the endogenous expression pattern of the endogenous gene. This mutant mouse strain represents a model of the nonsecretor ABH histo-blood group antigen, which confers resistance to Norwalk virus infection, and may be useful in studies of reproductive biology, gastrointestinal tract epithelium, and the function of fucosylated glycans.
This strain was transferred from the collection of the Consortium for Functional Glycomics.Development
A targeting vector containing neomycin resistance and beta-galactosidase genes was used to disrupt the exon encoding the catalytic domain. The construct was electroporated into 129X1/SvJ derived Pat5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were crossed to C57BL6J females. Heterozygotes were intercrossed to generate homozygotes. The mice were then backcrossed to C57BL/6J for 13 generations
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
Fut2tm1Sdo/Fut2tm1Sdo
B6.129X1-Fut2tm1Sdo
- reproductive system phenotype
- abnormal female reproductive system physiology (MGI Ref ID J:90993)
- fucosylated glycans absent in the endocervix and upper vagina
- immune system phenotype
- increased susceptibility to fungal infection (MGI Ref ID J:90993)
- increased susceptibility to vaginal infection with Candida albicans
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Fut2tm1Sdo/Fut2tm1Sdo
involves: 129X1/SvJ * C57BL/6J
- reproductive system phenotype
- abnormal female reproductive system physiology (MGI Ref ID J:89929)
- fucosylated glycolipids absent from female reproductive organs
- abnormal uterine environment (MGI Ref ID J:72946)
- absence of fucosylated glycans in the uterine epithelia but normal female fertility
- digestive/alimentary phenotype
- abnormal digestive system physiology (MGI Ref ID J:89929)
- fucosylated glycolipids absent from the colon
Research Applications
This mouse can be used to support research in many areas including:
Internal/Organ Research
Gastrointestinal Defects
Reproductive Biology Research
Research Tools
lacZ Expression
Genetics Research (Tissue/Cell Markers: multiple)
Reproductive Biology Research
Virology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Fut2tm1Sdo | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Steven E Domino | ||
| Common Name(s) | FUT2-; Fut2-LacZ; | ||
| Mutation Made By | Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI | ||
| Strain of Origin | 129X1/SvJ | ||
| ES Cell Line Name | Pat5 | ||
| ES Cell Line Strain | 129X1/SvJ | ||
| Site of Expression | lacZ expression patterns have not been characterized. | ||
| Gene Symbol and Name | Fut2, fucosyltransferase 2 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | Futb; SE; SEC2; Se2; sej; | ||
| General Note | The embryonic stem cell lines Pat5 and R1 were used for gene targeting. | ||
| Molecular Note | Most of the single coding exon of the gene was replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the catalytic domain. Homozygous mutant animals were identified by Southern blot and PCR genotype analysis. [MGI Ref ID J:72946] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fut2tm1Sdo, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Domino SE; Zhang L; Gillespie PJ; Saunders TL; Lowe JB. 2001. Deficiency of Reproductive Tract alpha(1,2)Fucosylated Glycans and Normal Fertility in Mice with Targeted Deletions of the FUT1 or FUT2 alpha(1,2)Fucosyltransferase Locus. Mol Cell Biol 21(24):8336-45. [PubMed: 11713270] [MGI Ref ID J:72946]
Additional References
Fut2tm1Sdo related
Domino SE; Hurd EA. 2004. LacZ expression in Fut2-LacZ reporter mice reveals estrogen-regulated endocervical glandular expression during estrous cycle, hormone replacement, and pregnancy. Glycobiology 14(2):169-75. [PubMed: 14576173] [MGI Ref ID J:105335]Domino SE; Karnak DM; Hurd EA. 2007. Cell surface fucosylation does not affect development of colon tumors in mice with germline Smad3 mutation. Tumour Biol 28(2):77-83. [PubMed: 17264540] [MGI Ref ID J:121796]
Hurd EA; Domino SE. 2004. Increased susceptibility of secretor factor gene Fut2-null mice to experimental vaginal candidiasis. Infect Immun 72(7):4279-81. [PubMed: 15213174] [MGI Ref ID J:90993]
Iwamori M; Domino SE. 2004. Tissue-specific loss of fucosylated glycolipids in mice with targeted deletion of alpha(1,2)fucosyltransferase genes. Biochem J 380(Pt 1):75-81. [PubMed: 14967068] [MGI Ref ID J:89929]
St John JA; Claxton C; Robinson MW; Yamamoto F; Domino SE; Key B. 2006. Genetic manipulation of blood group carbohydrates alters development and pathfinding of primary sensory axons of the olfactory systems. Dev Biol 298(2):470-84. [PubMed: 16884711] [MGI Ref ID J:119285]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Of note, homozygous mice have a chinchilla (gray) coat color, while heterozygotes have the typical black coat color expected for the C57BL/6J genetic background. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Fut2tm1Sdo *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Fut2tm1Sdo x Homozygous for Fut2tm1Sdo
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Fut2tm1Sdo *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Fut2tm1Sdo x Homozygous for Fut2tm1Sdo
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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