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| This strain is now distributed by the Mutant Mouse Regional Resource Center. Please refer to the Mutant Mouse Regional Resource Center (MMRRC) for ordering information and strain details on B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J/Mmjax
MMRRC Stock Number 034836. As a designated MMRRC center, The Jackson Laboratory will continue to distribute these mice at the same high health and quality standards but ordering is exclusively provided through the MMRRC. | ||||||||||||||||||||||
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- Disease & phenotype
- Genes & alleles
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Description
Strain Information
Former Names B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J (Changed: 11-AUG-11 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Lennart Mucke, Gladstone Inst of Neurological Disease Important Note
In March 2010, the donating investigator performed quantitative PCR on genomic DNA and determination of amyloid-beta levels in brain extract from the JAX Stock No. 006293 distribution colony (B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J also called "J20" line). Results of these analyses demonstrate that the current distribution colony maintains similar transgene copy number and expression as previously published (Mucke et. al. J Neurosci. 2000 Jun 1; 20(11): 4050-8).Description
These transgenic mice express a mutant form of the human amyloid protein precursor bearing both the Swedish (K670N/M671L) and the Indiana (V717F) mutations (APPSwInd). Expression of the transgenic insert is directed by the human platelet-derived growth factor beta polypeptide (PDGFB) promoter. Hemizygotes express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. Enzyme-linked immunosorbent assay (ELISA) analysis reveals approximate total amyloid beta peptides and 42 amino acid length amyloid beta peptides in neocortical and hippocampal tissue from mutant mice. At five to seven months of age diffuse amyloid beta peptides deposition in the dendate gyrus and neocortex forms. Amyloid deposition is progressive with all transgenic mice exhibiting plaques by age eight to 10 months. Video-EEG monitoring of 4 to 7 month old hemizygous transgenic mice, N10+ on the C57BL/6J background, reveals hippocampal hyperexcitability and cortical and hippocampal spontaneous nonconvulsive seizures. The mice are immobile, with no myoclonic behavior observed, during the non-convulsive electroencephalographic seizures. Pentylenetetrazole induced seizures have earlier onset, are more severe and result in more frequent deaths (50% develop fatal status epilepticus) than wildtype controls (Palop et al. Neuron 2007). This mutant mouse strain represents a model that may be useful in studies of the pathogenesis of Familial Alzheimer's Disease and possible therapeutic treatments.In March 2010, the donating investigator performed quantitative PCR on genomic DNA and determination of amyloid-beta levels in brain extract from B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J also called "J20" line. Results of these analyses demonstrate that the current distribution colony maintains similar transgene copy number and expression as previously published (Mucke et. al. J Neurosci. 2000 Jun 1; 20(11): 4050-8).
Development
A transgenic construct containing the mutant human amyloid protein precursor APPSwInd under the control of human platelet-derived growth factor beta polypeptide, simian sarcoma viral (v-sis) oncogene homolog, (PDGFB) promoter, was injected into C57BL/6 X DBA/2 F2 one-cell embryos. Heterozygous founder animals were bred to C57BL/6 X DBA/2 F1 mice. The resulting transgenic mice were then backcrossed for 12 generations on the C57BL/6J background.This strain was previously distributed as B6.Cg-Tg(PDGFB-APPSwInd)20Lms/1J. That strain was found to have undergone a loss of transgenic copy number and consequent delayed onset of the phenotype, so this strain was re-imported from the lab of Dr. Lennart Mucke. Transgenic copy number is assayed using a Multiplex TaqMan assay.
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Alzheimer's Disease Models
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Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Alzheimer Disease; AD
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Cerebral Amyloid Angiopathy, App-Related (APP)
assigned by genotype
Tg(PDGFB-APPSwInd)20Lms/0
B6.D2-Tg(PDGFB-APPSwInd)20Lms
- nervous system phenotype
- abnormal neuron morphology
- dystrophic neurites are associated with plaques (MGI Ref ID J:100954)
- amyloid beta deposits
- mice develop amyloid peptide deposits by 5-7 months of age (MGI Ref ID J:100954)
- other phenotype
- amyloid beta deposits
- mice develop amyloid peptide deposits by 5-7 months of age (MGI Ref ID J:100954)
Tg(PDGFB-APPSwInd)20Lms/0
B6.Cg-Tg(PDGFB-APPSwInd)20Lms
- nervous system phenotype
- abnormal cerebral cortex pyramidal cell morphology
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(PDGFB-APPSwInd)20Lms/0
involves: C57BL/6 * DBA/2
- mortality/aging
- premature death (MGI Ref ID J:141084)
- behavior/neurological phenotype
- abnormal long term object recognition memory
- at 4-7 months of age, mice transgenic mice with wild-type Mapt expression take longer to locate the platform in the cued version of the Morris water maze test compared to transgenic mice heterozygous or homozygous for Mapt deletion (MGI Ref ID J:121330)
- abnormal spatial learning
- in the hidden platform version of the Morris water maze, non-trangenic controls, regardless of Mapt genotype learn the task over 3 days, whereas transgenic mice with wild-type expression of Mapt show no evidence of learning (MGI Ref ID J:121330)
- in probe trials where the platform is removed, mice show no learning, displaying no significantly elevated crossings of the target quadrant after 5 days of training (MGI Ref ID J:121330)
- in a Morris water maze, mice fail to favor the target platform location unlike wild-type mice (MGI Ref ID J:141084)
- decreased anxiety-related response
- mice exhibit disinhibition-like behaviors in an elevated plus maze compared with wild-type mice (MGI Ref ID J:141084)
- hyperactivity (MGI Ref ID J:141084)
- mice show hyperactivity in the Y-maze, a new cage, and elevated-plus maze compared to other transgenic mice or non-transgenic controls; this persists in mice 12-16 months of age (MGI Ref ID J:121330)
- increased susceptibility to pharmacologically induced seizures
- mice are abnormally sensitive to pentylenetetrazole (PTZ)-induced seizures with 20% suffering fatal status epilepticus at a PTZ dose not lethal in non-transgenic controls (MGI Ref ID J:121330)
- nervous system phenotype
- abnormal neuron morphology
- mice show neuritic dystrophy around amyloid plaques (MGI Ref ID J:121330)
- abnormal neuron differentiation
- aberrant sprouting of hippocampal axons is observed in transgenic mice (MGI Ref ID J:121330)
- amyloid beta deposits
- diffuse immunoreactive amyloid deposits detected in dentate gyrus and neocortex of mice aged 5 to 7 months old (MGI Ref ID J:62290)
- all mice exhibit plaques by age 8 to 10 months (MGI Ref ID J:62290)
- at 4-7 months and 14-18 months, Abeta plaque deposition is observed, at levels the same as other transgenics heterozygous null for Mapt (MGI Ref ID J:121330)
- soluble and insoluble Abeta-40 and -42 peptide deposits at 6-10 months (MGI Ref ID J:87150)
- increased susceptibility to pharmacologically induced seizures
- mice are abnormally sensitive to pentylenetetrazole (PTZ)-induced seizures with 20% suffering fatal status epilepticus at a PTZ dose not lethal in non-transgenic controls (MGI Ref ID J:121330)
- neurodegeneration
- neurodegeneration is indicated by an age dependent decrease in density of synaptophysin-immunoreactive presynaptic terminals (MGI Ref ID J:62290)
- other phenotype
- amyloid beta deposits
- diffuse immunoreactive amyloid deposits detected in dentate gyrus and neocortex of mice aged 5 to 7 months old (MGI Ref ID J:62290)
- all mice exhibit plaques by age 8 to 10 months (MGI Ref ID J:62290)
- at 4-7 months and 14-18 months, Abeta plaque deposition is observed, at levels the same as other transgenics heterozygous null for Mapt (MGI Ref ID J:121330)
- soluble and insoluble Abeta-40 and -42 peptide deposits at 6-10 months (MGI Ref ID J:87150)
- cellular phenotype
- abnormal neuron differentiation
- aberrant sprouting of hippocampal axons is observed in transgenic mice (MGI Ref ID J:121330)
This mouse can be used to support research in many areas including:
APP relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
strains expressing mutant APP
Behavioral and Learning Defects
Epilepsy
electroconvulsive seizures
increased susceptibility to kainate-induced seizures
Tg(PDGFB-APPSwInd)20Lms relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Neurodegeneration
Neurobiology Research
Alzheimer's Disease
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(PDGFB-APPSwInd)20Lms | ||
|---|---|---|---|
| Allele Name | transgene insertion 20, Lennart Mucke | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | APP Tg; APP/J20; J20; PDGF-APPSwInd; PDGF-hAPP695,751,770V171F, KM670/671NL; hAPPJ20; | ||
| Mutation Made By | Lennart Mucke, Gladstone Inst of Neurological Disease | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | APP, amyloid beta (A4) precursor protein, human | ||
| Promoter | PDGFB, platelet-derived growth factor beta polypeptide, human | ||
| General Note |
This line is also called line J20. Diffuse amyloid beta peptides deposition forms in the dendate gyrus and neocortex at 5 to 7 months of age. Amyloid deposition is progressive, with all transgenic mice exhibiting plaques by age 8 to 10 months. | ||
| Molecular Note | The transgene expresses the mutant human amyloid protein precursor APPSwInd, which bears both the Swedish (K670N/M671L) and the Indiana (V717F) mutations, under the control of the human platelet derived growth factor, B polypeptide (PDGFB) promoter. Hemizygous transgenic mice express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. ELISA analysis reveals approximate total amyloid beta peptides and 42 amino acid lengthamyloid beta peptides in neocortical and hippocampal tissue from transgenic mice. [MGI Ref ID J:62290] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(APP), QPCR
Tg(PDGFB-APP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Mucke L; Masliah E; Yu GQ; Mallory M; Rockenstein EM; Tatsuno G; Hu K; Kholodenko D; Johnson-Wood K; McConlogue L. 2000. High-level neuronal expression of abeta 1-42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation. J Neurosci 20(11):4050-8. [PubMed: 10818140] [MGI Ref ID J:62290]
Additional References
Moreno H; Wu WE; Lee T; Brickman A; Mayeux R; Brown TR; Small SA. 2007. Imaging the abeta-related neurotoxicity of Alzheimer disease. Arch Neurol 64(10):1467-77. [PubMed: 17923630] [MGI Ref ID J:125332]
Tg(PDGFB-APPSwInd)20Lms relatedAucoin JS; Jiang P; Aznavour N; Tong XK; Buttini M; Descarries L; Hamel E. 2005. Selective cholinergic denervation, independent from oxidative stress, in a mouse model of Alzheimer's disease. Neuroscience 132(1):73-86. [PubMed: 15780468] [MGI Ref ID J:97281]
Baron R; Harpaz I; Nemirovsky A; Cohen H; Monsonego A. 2007. Immunity and neuronal repair in the progression of Alzheimer's disease: a brief overview. Exp Gerontol 42(1-2):64-9. [PubMed: 17074458] [MGI Ref ID J:123181]
Baron R; Nemirovsky A; Harpaz I; Cohen H; Owens T; Monsonego A. 2008. IFN-gamma enhances neurogenesis in wild-type mice and in a mouse model of Alzheimer's disease. FASEB J 22(8):2843-52. [PubMed: 18390924] [MGI Ref ID J:138024]
Belkacemi A; Ramassamy C. 2012. Time sequence of oxidative stress in the brain from transgenic mouse models of Alzheimer's disease related to the amyloid-beta cascade. Free Radic Biol Med 52(3):593-600. [PubMed: 22172527] [MGI Ref ID J:180298]
Bien-Ly N; Andrews-Zwilling Y; Xu Q; Bernardo A; Wang C; Huang Y. 2011. C-terminal-truncated apolipoprotein (apo) E4 inefficiently clears amyloid-beta (Abeta) and acts in concert with Abeta to elicit neuronal and behavioral deficits in mice. Proc Natl Acad Sci U S A 108(10):4236-41. [PubMed: 21368138] [MGI Ref ID J:170827]
Bien-Ly N; Gillespie AK; Walker D; Yoon SY; Huang Y. 2012. Reducing human apolipoprotein E levels attenuates age-dependent Abeta accumulation in mutant human amyloid precursor protein transgenic mice. J Neurosci 32(14):4803-11. [PubMed: 22492035] [MGI Ref ID J:184138]
Buckwalter MS; Coleman BS; Buttini M; Barbour R; Schenk D; Games D; Seubert P; Wyss-Coray T. 2006. Increased T cell recruitment to the CNS after amyloid beta 1-42 immunization in Alzheimer's mice overproducing transforming growth factor-beta 1. J Neurosci 26(44):11437-41. [PubMed: 17079673] [MGI Ref ID J:114703]
Caspersen C; Wang N; Yao J; Sosunov A; Chen X; Lustbader JW; Xu HW; Stern D; McKhann G; Yan SD. 2005. Mitochondrial Abeta: a potential focal point for neuronal metabolic dysfunction in Alzheimer's disease. FASEB J 19(14):2040-1. [PubMed: 16210396] [MGI Ref ID J:127883]
Cheng IH; Palop JJ; Esposito LA; Bien-Ly N; Yan F; Mucke L. 2004. Aggressive amyloidosis in mice expressing human amyloid peptides with the Arctic mutation. Nat Med 10(11):1190-2. [PubMed: 15502844] [MGI Ref ID J:100954]
Cheng IH; Scearce-Levie K; Legleiter J; Palop JJ; Gerstein H; Bien-Ly N; Puolivali J; Lesne S; Ashe KH; Muchowski PJ; Mucke L. 2007. Accelerating amyloid-beta fibrillization reduces oligomer levels and functional deficits in Alzheimer disease mouse models. J Biol Chem 282(33):23818-28. [PubMed: 17548355] [MGI Ref ID J:124712]
Cheng JS; Dubal DB; Kim DH; Legleiter J; Cheng IH; Yu GQ; Tesseur I; Wyss-Coray T; Bonaldo P; Mucke L. 2009. Collagen VI protects neurons against Abeta toxicity. Nat Neurosci 12(2):119-21. [PubMed: 19122666] [MGI Ref ID J:145982]
Chin J; Massaro CM; Palop JJ; Thwin MT; Yu GQ; Bien-Ly N; Bender A; Mucke L. 2007. Reelin depletion in the Enthorhinal cortex of human amyloid precursor protein transgenic mice and humans with Alzheimer's disease J Neurosci 27(11):2727-2733. [PubMed: 17360894] [MGI Ref ID J:118589]
Chin J; Palop JJ; Puolivali J; Massaro C; Bien-Ly N; Gerstein H; Scearce-Levie K; Masliah E; Mucke L. 2005. Fyn kinase induces synaptic and cognitive impairments in a transgenic mouse model of Alzheimer's disease. J Neurosci 25(42):9694-703. [PubMed: 16237174] [MGI Ref ID J:102161]
Chin J; Palop JJ; Yu GQ; Kojima N; Masliah E; Mucke L. 2004. Fyn kinase modulates synaptotoxicity, but not aberrant sprouting, in human amyloid precursor protein transgenic mice. J Neurosci 24(19):4692-7. [PubMed: 15140940] [MGI Ref ID J:96892]
Choi SW; Gerencser AA; Ng R; Flynn JM; Melov S; Danielson SR; Gibson BW; Nicholls DG; Bredesen DE; Brand MD. 2012. No consistent bioenergetic defects in presynaptic nerve terminals isolated from mouse models of Alzheimer's disease. J Neurosci 32(47):16775-84. [PubMed: 23175831] [MGI Ref ID J:192812]
Cisse M; Halabisky B; Harris J; Devidze N; Dubal DB; Sun B; Orr A; Lotz G; Kim DH; Hamto P; Ho K; Yu GQ; Mucke L. 2011. Reversing EphB2 depletion rescues cognitive functions in Alzheimer model. Nature 469(7328):47-52. [PubMed: 21113149] [MGI Ref ID J:167228]
Cisse M; Sanchez PE; Kim DH; Ho K; Yu GQ; Mucke L. 2011. Ablation of cellular prion protein does not ameliorate abnormal neural network activity or cognitive dysfunction in the j20 line of human amyloid precursor protein transgenic mice. J Neurosci 31(29):10427-31. [PubMed: 21775587] [MGI Ref ID J:174512]
Davis AA; Fritz JJ; Wess J; Lah JJ; Levey AI. 2010. Deletion of M1 muscarinic acetylcholine receptors increases amyloid pathology in vitro and in vivo. J Neurosci 30(12):4190-6. [PubMed: 20335454] [MGI Ref ID J:159221]
Deipolyi AR; Fang S; Palop JJ; Yu GQ; Wang X; Mucke L. 2008. Altered navigational strategy use and visuospatial deficits in hAPP transgenic mice. Neurobiol Aging 29(2):253-66. [PubMed: 17126954] [MGI Ref ID J:130693]
Du H; Guo L; Yan S; Sosunov AA; McKhann GM; Yan SS. 2010. Early deficits in synaptic mitochondria in an Alzheimer's disease mouse model. Proc Natl Acad Sci U S A 107(43):18670-5. [PubMed: 20937894] [MGI Ref ID J:165506]
Escribano L; Simon AM; Perez-Mediavilla A; Salazar-Colocho P; Del Rio J; Frechilla D. 2009. Rosiglitazone reverses memory decline and hippocampal glucocorticoid receptor down-regulation in an Alzheimer's disease mouse model. Biochem Biophys Res Commun 379(2):406-10. [PubMed: 19109927] [MGI Ref ID J:144519]
Esposito L; Raber J; Kekonius L; Yan F; Yu GQ; Bien-Ly N; Puolivali J; Scearce-Levie K; Masliah E; Mucke L. 2006. Reduction in mitochondrial superoxide dismutase modulates Alzheimer's disease-like pathology and accelerates the onset of behavioral changes in human amyloid precursor protein transgenic mice. J Neurosci 26(19):5167-79. [PubMed: 16687508] [MGI Ref ID J:108685]
Fang F; Chen X; Huang T; Lue LF; Luddy JS; Yan SS. 2012. Multi-faced neuroprotective effects of Ginsenoside Rg1 in an Alzheimer mouse model. Biochim Biophys Acta 1822(2):286-92. [PubMed: 22015470] [MGI Ref ID J:180329]
Fisher Y; Nemirovsky A; Baron R; Monsonego A. 2010. T cells specifically targeted to amyloid plaques enhance plaque clearance in a mouse model of Alzheimer's disease. PLoS One 5(5):e10830. [PubMed: 20520819] [MGI Ref ID J:160898]
Gaikwad S; Larionov S; Wang Y; Dannenberg H; Matozaki T; Monsonego A; Thal DR; Neumann H. 2009. Signal regulatory protein-beta1: a microglial modulator of phagocytosis in Alzheimer's disease. Am J Pathol 175(6):2528-39. [PubMed: 19893026] [MGI Ref ID J:155330]
Garcia-Barroso C; Ricobaraza A; Pascual-Lucas M; Unceta N; Rico AJ; Goicolea MA; Salles J; Lanciego JL; Oyarzabal J; Franco R; Cuadrado-Tejedor M; Garcia-Osta A. 2013. Tadalafil crosses the blood-brain barrier and reverses cognitive dysfunction in a mouse model of AD. Neuropharmacology 64:114-23. [PubMed: 22776546] [MGI Ref ID J:192668]
Griffiths HH; Whitehouse IJ; Baybutt H; Brown D; Kellett KA; Jackson CD; Turner AJ; Piccardo P; Manson JC; Hooper NM. 2011. Prion Protein Interacts with BACE1 Protein and Differentially Regulates Its Activity toward Wild Type and Swedish Mutant Amyloid Precursor Protein. J Biol Chem 286(38):33489-500. [PubMed: 21795680] [MGI Ref ID J:176738]
Gultner S; Laue M; Riemer C; Heise I; Baier M. 2009. Prion disease development in slow Wallerian degeneration (Wld(S)) mice. Neurosci Lett 456(2):93-8. [PubMed: 19429141] [MGI Ref ID J:150443]
Harris JA; Devidze N; Halabisky B; Lo I; Thwin MT; Yu GQ; Bredesen DE; Masliah E; Mucke L. 2010. Many neuronal and behavioral impairments in transgenic mouse models of Alzheimer's disease are independent of caspase cleavage of the amyloid precursor protein. J Neurosci 30(1):372-81. [PubMed: 20053918] [MGI Ref ID J:157639]
Ho A; Liu X; Sudhof TC. 2008. Deletion of Mint proteins decreases amyloid production in transgenic mouse models of Alzheimer's disease. J Neurosci 28(53):14392-400. [PubMed: 19118172] [MGI Ref ID J:142874]
Hong S; Quintero-Monzon O; Ostaszewski BL; Podlisny DR; Cavanaugh WT; Yang T; Holtzman DM; Cirrito JR; Selkoe DJ. 2011. Dynamic Analysis of Amyloid beta-Protein in Behaving Mice Reveals Opposing Changes in ISF versus Parenchymal Abeta during Age-Related Plaque Formation. J Neurosci 31(44):15861-9. [PubMed: 22049429] [MGI Ref ID J:177845]
Hook VY; Kindy M; Reinheckel T; Peters C; Hook G. 2009. Genetic cathepsin B deficiency reduces beta-amyloid in transgenic mice expressing human wild-type amyloid precursor protein. Biochem Biophys Res Commun 386(2):284-8. [PubMed: 19501042] [MGI Ref ID J:151423]
Hosono-Fukao T; Ohtake-Niimi S; Hoshino H; Britschgi M; Akatsu H; Hossain MM; Nishitsuji K; van Kuppevelt TH; Kimata K; Michikawa M; Wyss-Coray T; Uchimura K. 2012. Heparan Sulfate Subdomains that are Degraded by Sulf Accumulate in Cerebral Amyloid ss Plaques of Alzheimer's Disease: Evidence from Mouse Models and Patients. Am J Pathol 180(5):2056-67. [PubMed: 22429964] [MGI Ref ID J:183381]
Kalinin S; Gavrilyuk V; Polak PE; Vasser R; Zhao J; Heneka MT; Feinstein DL. 2007. Noradrenaline deficiency in brain increases beta-amyloid plaque burden in an animal model of Alzheimer's disease. Neurobiol Aging 28(8):1206-14. [PubMed: 16837104] [MGI Ref ID J:123899]
Karl T; Bhatia S; Cheng D; Kim WS; Garner B. 2012. Cognitive phenotyping of amyloid precursor protein transgenic J20 mice. Behav Brain Res 228(2):392-7. [PubMed: 22197298] [MGI Ref ID J:181714]
Kitaguchi H; Tomimoto H; Ihara M; Shibata M; Uemura K; Kalaria RN; Kihara T; Asada-Utsugi M; Kinoshita A; Takahashi R. 2009. Chronic cerebral hypoperfusion accelerates amyloid beta deposition in APPSwInd transgenic mice. Brain Res 1294:202-10. [PubMed: 19646974] [MGI Ref ID J:157205]
Larson ME; Sherman MA; Greimel S; Kuskowski M; Schneider JA; Bennett DA; Lesne SE. 2012. Soluble alpha-Synuclein Is a Novel Modulator of Alzheimer's Disease Pathophysiology. J Neurosci 32(30):10253-66. [PubMed: 22836259] [MGI Ref ID J:186543]
Leissring MA; Farris W; Chang AY; Walsh DM; Wu X; Sun X; Frosch MP; Selkoe DJ. 2003. Enhanced proteolysis of beta-amyloid in APP transgenic mice prevents plaque formation, secondary pathology, and premature death. Neuron 40(6):1087-93. [PubMed: 14687544] [MGI Ref ID J:87150]
Liu R; Lei JX; Luo C; Lan X; Chi L; Deng P; Lei S; Ghribi O; Liu QY. 2012. Increased EID1 nuclear translocation impairs synaptic plasticity and memory function associated with pathogenesis of Alzheimer's disease. Neurobiol Dis 45(3):902-12. [PubMed: 22186421] [MGI Ref ID J:182043]
Lourenco FC; Galvan V; Fombonne J; Corset V; Llambi F; Muller U; Bredesen DE; Mehlen P. 2009. Netrin-1 interacts with amyloid precursor protein and regulates amyloid-beta production. Cell Death Differ 16(5):655-63. [PubMed: 19148186] [MGI Ref ID J:164195]
Maier M; Peng Y; Jiang L; Seabrook TJ; Carroll MC; Lemere CA. 2008. Complement C3 deficiency leads to accelerated amyloid beta plaque deposition and neurodegeneration and modulation of the microglia/macrophage phenotype in amyloid precursor protein transgenic mice. J Neurosci 28(25):6333-41. [PubMed: 18562603] [MGI Ref ID J:135902]
Meilandt WJ ; Cisse M ; Ho K ; Wu T ; Esposito LA ; Scearce-Levie K ; Cheng IH ; Yu GQ ; Mucke L. 2009. Neprilysin overexpression inhibits plaque formation but fails to reduce pathogenic Abeta oligomers and associated cognitive deficits in human amyloid precursor protein transgenic mice. J Neurosci 29(7):1977-86. [PubMed: 19228952] [MGI Ref ID J:146594]
Meilandt WJ; Yu GQ; Chin J; Roberson ED; Palop JJ; Wu T; Scearce-Levie K; Mucke L. 2008. Enkephalin elevations contribute to neuronal and behavioral impairments in a transgenic mouse model of Alzheimer's disease. J Neurosci 28(19):5007-17. [PubMed: 18463254] [MGI Ref ID J:135174]
Monsonego A; Imitola J; Petrovic S; Zota V; Nemirovsky A; Baron R; Fisher Y; Owens T; Weiner HL. 2006. Abeta-induced meningoencephalitis is IFN-gamma-dependent and is associated with T cell-dependent clearance of Abeta in a mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A 103(13):5048-53. [PubMed: 16549802] [MGI Ref ID J:107636]
Moreno H; Wu WE; Lee T; Brickman A; Mayeux R; Brown TR; Small SA. 2007. Imaging the abeta-related neurotoxicity of Alzheimer disease. Arch Neurol 64(10):1467-77. [PubMed: 17923630] [MGI Ref ID J:125332]
Mueller-Steiner S; Zhou Y; Arai H; Roberson ED; Sun B; Chen J; Wang X; Yu G; Esposito L; Mucke L; Gan L. 2006. Antiamyloidogenic and neuroprotective functions of cathepsin B: implications for Alzheimer's disease. Neuron 51(6):703-14. [PubMed: 16982417] [MGI Ref ID J:113649]
Murakami K; Yokoyama S; Murata N; Ozawa Y; Irie K; Shirasawa T; Shimizu T. 2011. Insulin receptor mutation results in insulin resistance and hyperinsulinemia but does not exacerbate Alzheimer's-like phenotypes in mice. Biochem Biophys Res Commun 409(1):34-9. [PubMed: 21549686] [MGI Ref ID J:172366]
Ongali B; Nicolakakis N; Lecrux C; Aboulkassim T; Rosa-Neto P; Papadopoulos P; Tong XK; Hamel E. 2010. Transgenic mice overexpressing APP and transforming growth factor-beta1 feature cognitive and vascular hallmarks of Alzheimer's disease. Am J Pathol 177(6):3071-80. [PubMed: 21088218] [MGI Ref ID J:167619]
Palop JJ; Chin J; Roberson ED; Wang J; Thwin MT; Bien-Ly N; Yoo J; Ho KO; Yu GQ; Kreitzer A; Finkbeiner S; Noebels JL; Mucke L. 2007. Aberrant excitatory neuronal activity and compensatory remodeling of inhibitory hippocampal circuits in mouse models of Alzheimer's disease. Neuron 55(5):697-711. [PubMed: 17785178] [MGI Ref ID J:126808]
Palop JJ; Jones B; Kekonius L; Chin J; Yu GQ; Raber J; Masliah E; Mucke L. 2003. Neuronal depletion of calcium-dependent proteins in the dentate gyrus is tightly linked to Alzheimer's disease-related cognitive deficits. Proc Natl Acad Sci U S A 100(16):9572-7. [PubMed: 12881482] [MGI Ref ID J:106227]
Pickford F; Masliah E; Britschgi M; Lucin K; Narasimhan R; Jaeger PA; Small S; Spencer B; Rockenstein E; Levine B; Wyss-Coray T. 2008. The autophagy-related protein beclin 1 shows reduced expression in early Alzheimer disease and regulates amyloid beta accumulation in mice. J Clin Invest 118(6):2190-9. [PubMed: 18497889] [MGI Ref ID J:137727]
Poirier R; Wolfer DP; Welzl H; Tracy J; Galsworthy MJ; Nitsch RM; Mohajeri MH. 2006. Neuronal neprilysin overexpression is associated with attenuation of Abeta-related spatial memory deficit. Neurobiol Dis 24(3):475-83. [PubMed: 17008108] [MGI Ref ID J:147325]
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Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous males are bred to wildtype siblings or to C57BL/6J inbred females. Pups born of carrier females have shown an increased mortality rate in our colonies.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
This strain is currently Transferred.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note
In March 2010, the donating investigator performed quantitative PCR on genomic DNA and determination of amyloid-beta levels in brain extract from the JAX Stock No. 006293 distribution colony (B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J also called "J20" line). Results of these analyses demonstrate that the current distribution colony maintains similar transgene copy number and expression as previously published (Mucke et. al. J Neurosci. 2000 Jun 1; 20(11): 4050-8).
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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