Strain Name: |
B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J |
|---|---|
Stock Number: |
006293 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | J20 line; |
| Genes & Alleles | APP; PDGFB; Tg(PDGFB-APPSwInd)20Lms; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Lennart Mucke, Gladstone Inst of Neurological Disease Generation N12+3 (03-JUN-07) Strain Description
These transgenic mice express a mutant form of the human amyloid protein precursor bearing both the Swedish (K670N/M671L) and the Indiana (V717F) mutations (APPSwInd). Expression of the transgenic insert is directed by the human platelet-derived growth factor beta polypeptide (PDGFB) promoter. Hemizygotes express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. Enzyme-linked immunosorbent assay (ELISA) analysis reveals approximate total amyloid beta peptides and 42 amino acid length amyloid beta peptides in neocortical and hippocampal tissue from mutant mice. At 5 to 7 months of age diffuse amyloid beta peptides deposition in the dendate gyrus and neocortex forms. Amyloid deposition is progressive with all transgenic mice exhibiting plaques by age 8 to 10 months. This mutant mouse strain represents a model that may be useful in studies of the pathogenesis of Familial Alzheimer's Disease and possible therapeutic treatments.Strain Development
A transgenic construct containing the mutant human amyloid protein precursor APPSwInd under the control of human platelet-derived growth factor beta polypeptide, simian sarcoma viral (v-sis) oncogene homolog, (PDGFB) promoter, was injected into C57BL/6 X DBA/2 F2 one-cell embryos. Heterozygous founder animals were bred to C57BL/6 X DBA/2 F1 mice. The resulting transgenic mice were then backcrossed for 12 generations on the C57BL/6J background.This strain was previously distributed as Stock No. 004661. That strain was found to have undergone a loss of transgenic copy number and consequent delayed onset of the phenotype, so this strain was re-imported from the lab of Dr. Lennart Mucke. Transgenic copy number is assayed using a Multiplex TaqMan assay.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(PDGFB-APPSwInd)20Lms | ||
|---|---|---|---|
| Allele Name | transgene insertion 20, Lennart Mucke | ||
| Common Name(s) | J20; PDGF-APPSwInd; | ||
| Mutation Made By | Lennart Mucke, Gladstone Inst of Neurological Disease | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | APP, amyloid beta (A4) precursor protein, human | ||
| Promoter | PDGFB, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), human | ||
| General Note |
This line is also called line J20. Diffuse amyloid beta peptides deposition forms in the dendate gyrus and neocortex at 5 to 7 months of age. Amyloid deposition is progressive, with all transgenic mice exhibiting plaques by age 8 to 10 months. | ||
| Molecular Note | The transgene expresses the mutant human amyloid protein precursor APPSwInd, which bears both the Swedish (K670N/M671L) and the Indiana (V717F) mutations, under the control of the human platelet derived growth factor, B polypeptide (PDGFB) promoter. Hemizygous transgenic mice express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. ELISA analysis reveals approximate total amyloid beta peptides and 42 amino acid lengthamyloid beta peptides in neocortical and hippocampal tissue from transgenic mice. [MGI Ref ID J:62290] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Tg(PDGFB-APP)
| Breeding & Husbandry | When maintaining a live colony, hemizygous males are bred to wildtype siblings or to C57BL/6J inbred females. Pups born of carrier females have shown an increased mortality rate in our colonies. |
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| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying Tg(PDGFB-APPSwInd)20Lms allele
004661 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/1J View Strains carrying Tg(PDGFB-APPSwInd)20Lms (1 strain)
Strains carrying other alleles of APP
006409 129S1.Cg-Tg(APPSw)40Btla/J 006555 A.129(B6)-Tg(APPSw)40Btla/J 005300 B6.129-Tg(APPSw)40Btla/J 005301 B6.129S2-Tg(APP)8.9Btla/J 006406 B6.129S4-Tg(APPSwLon)96Btla/J 005864 B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/J 004662 B6.Cg-Tg(PDGFB-APP)5Lms/J 006006 B6.Cg-Tg(Prnp-APP)A-2Dbo/J 006005 B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J 007049 B6.Cg-Tg(tetO-APPSwInd)885Dbo/J 004462 B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J 006004 B6C3-Tg(tetO-APPSwInd)885Dbo/J 007027 C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/J 006472 D2.129(B6)-Tg(APPSw)40Btla/J View Strains carrying other alleles of APP (14 strains)
Strains carrying other alleles of PDGFB
004662 B6.Cg-Tg(PDGFB-APP)5Lms/J View Strains carrying other alleles of PDGFB (1 strain)
Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Room Number AX11
APP relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease (strains expressing mutant APP)
Alzheimer's Disease
Behavioral and Learning Defects
Tg(PDGFB-APPSwInd)20Lms relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Neurodegeneration
Neurobiology Research
Alzheimer's Disease
Selected Reference(s)
Additional ReferencesMucke L; Masliah E; Yu GQ; Mallory M; Rockenstein EM; Tatsuno G; Hu K; Kholodenko D; Johnson-Wood K; McConlogue L. 2000. High-level neuronal expression of abeta 1-42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation. J Neurosci 20(11):4050-8. [PubMed: 10818140] [MGI Ref ID J:62290]
| Strain Name: | B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J |
| Stock Number: | 006293 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
AGED MICE WILL BE AVAILABLE. Limited quantities of 6 month old animals will be available. Cost will be the listed price per animal, plus a charge based on the age of the animal. To register interest in obtaining aged animals, go to the Strain Interest Form. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE only available to non-profit entities.
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