Strain Name:

B6.129S-Id3tm1Zhu/J

Stock Number:

006301

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN11+N1F2pN1
Generation Definitions
 
Donating Investigator Yuan Zhuang,   Duke University Medical Center

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot analysis from spleen tissue. Homozygotes exhibit reduced basal IgG1 and IgG2a levels. When challenged with keyhole limpet hemocyanin coupled to DNP, production of IgG2a and IgG3 was 10% and 6% of the wildtype response after primary and secondary challenges, respectively. DNP-Ficoll challenge results in a 90% reduction of IgM and IgA production. Cultured B-cells have diminished proliferation in response to surface IgM stimulation. Keratoconjunctivitis sicca (dry eyes) occurs in 10% of homozygotes aged 6 to 10 months, and in 80% of mice more than 1 year in age. Tear and saliva flow volume is reduced. Histological analysis reveals lymphocyte infiltration and tissue degradation in lachrymal and salivary glands. Mutants older than 1 year in age produce autoantibodies, anti-SSA and anti-SSB. This mutant mouse strain may be useful in studies of primary Sjogren syndrome.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exons 1 and 2 and part of exon 3. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 11 generations.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Id3
024496   B6.129S6(Cg)-Id3tm2.1Zhu/J
010983   B6;129S-Id3tm1Pzg/J
View Strains carrying other alleles of Id3     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.
Sjogren Syndrome
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Id3tm1Zhu/Id3tm1Zhu

        B6.129S4-Id3tm1Zhu
  • endocrine/exocrine gland phenotype
  • abnormal lacrimal gland physiology
    • as early as 2 months of age, mice exhibit reduced tear production compared with wild-type mice   (MGI Ref ID J:93916)
  • decreased salivation
    • as early as 2 months of age   (MGI Ref ID J:93916)
  • digestive/alimentary phenotype
  • decreased salivation
    • as early as 2 months of age   (MGI Ref ID J:93916)
  • vision/eye phenotype
  • abnormal lacrimal gland physiology
    • as early as 2 months of age, mice exhibit reduced tear production compared with wild-type mice   (MGI Ref ID J:93916)
  • homeostasis/metabolism phenotype
  • decreased salivation
    • as early as 2 months of age   (MGI Ref ID J:93916)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Id3tm1Zhu/Id3tm1Zhu

        involves: 129/Sv * C57BL/6
  • immune system phenotype
  • *normal* immune system phenotype
    • normal numbers of splenocytes and thymocytes   (MGI Ref ID J:56950)
    • basal levels of IgM, IgG2b, IgG3, and IgA are normal; after challenge IgM, IgG1, IgG2b, IgA levels are as expected   (MGI Ref ID J:56950)
    • abnormal T cell physiology
      • IFN-gamma expression reduced after T-cell stimulation   (MGI Ref ID J:56950)
    • decreased B cell proliferation
      • reduced response to anti-IgM   (MGI Ref ID J:56950)
    • decreased immunoglobulin level
      • basal levels of IgG1 and IgG2a reduced to 1/2 normal   (MGI Ref ID J:56950)
      • after primary challenge, IgG2a and IgG3 levels about 1/10 normal   (MGI Ref ID J:56950)
      • after secondary challenge, levels are 1/15 normal   (MGI Ref ID J:56950)
      • when challenged with DNP-Ficoll, specific IgM and IgA levels 1/10 normal   (MGI Ref ID J:56950)
  • hematopoietic system phenotype
  • abnormal T cell physiology
    • IFN-gamma expression reduced after T-cell stimulation   (MGI Ref ID J:56950)
  • decreased B cell proliferation
    • reduced response to anti-IgM   (MGI Ref ID J:56950)
  • decreased immunoglobulin level
    • basal levels of IgG1 and IgG2a reduced to 1/2 normal   (MGI Ref ID J:56950)
    • after primary challenge, IgG2a and IgG3 levels about 1/10 normal   (MGI Ref ID J:56950)
    • after secondary challenge, levels are 1/15 normal   (MGI Ref ID J:56950)
    • when challenged with DNP-Ficoll, specific IgM and IgA levels 1/10 normal   (MGI Ref ID J:56950)

Id3tm1Zhu/Id3tm1Zhu

        involves: 129S4/SvJaeSor * C57BL/6
  • hematopoietic system phenotype
  • abnormal T cell differentiation
    • serial transplant of mutant bone marrow into irradiated hosts reveals a defect in thymocyte progenitors   (MGI Ref ID J:144696)
    • secondary transplant of mutant bone marrow leads to an 8-fold reduction in the thymocytes compared to controls 5 weeks after engraftment, and 3-fold less thymocytes are present 12 days after engraftment   (MGI Ref ID J:144696)
    • increasing number of bone marrow cells transplanted still fail to rescue the defect suggesting a defect occurs during T cell development   (MGI Ref ID J:144696)
  • immune system phenotype
  • abnormal T cell differentiation
    • serial transplant of mutant bone marrow into irradiated hosts reveals a defect in thymocyte progenitors   (MGI Ref ID J:144696)
    • secondary transplant of mutant bone marrow leads to an 8-fold reduction in the thymocytes compared to controls 5 weeks after engraftment, and 3-fold less thymocytes are present 12 days after engraftment   (MGI Ref ID J:144696)
    • increasing number of bone marrow cells transplanted still fail to rescue the defect suggesting a defect occurs during T cell development   (MGI Ref ID J:144696)

Id3tm1Zhu/Id3tm1Zhu

        involves: 129S4/SvJaeSor
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • mice are healthy and fertile   (MGI Ref ID J:44278)
  • endocrine/exocrine gland phenotype
  • abnormal lacrimal gland morphology
    • aged mice exhibit destruction of lacrimal gland tissue with opaque spots unlike wild-type mice   (MGI Ref ID J:93916)
  • abnormal lacrimal gland physiology
    • as early as 2 to 4 months of age, mice exhibit reduced tear production compared with wild-type mice   (MGI Ref ID J:93916)
    • however, thymectomy rescues defect   (MGI Ref ID J:93916)
    • lacrimal gland inflammation
      • as early as 2 months of age prior to eye abnormalities   (MGI Ref ID J:93916)
  • decreased salivation
    • as early as 2 to 4 months of age   (MGI Ref ID J:93916)
    • however, thymectomy rescues defect   (MGI Ref ID J:93916)
  • salivary gland inflammation
    • as early as 2 months of age and increasing in severity with age   (MGI Ref ID J:93916)
  • immune system phenotype
  • *normal* immune system phenotype
    • peripheral lymphocytes are normal   (MGI Ref ID J:93916)
    • increased autoantibody level
      • at 1 year of age, mice exhibit increased levels of SSA and SSB autoantibodies compared with wild-type mice   (MGI Ref ID J:93916)
    • lacrimal gland inflammation
      • as early as 2 months of age prior to eye abnormalities   (MGI Ref ID J:93916)
    • salivary gland inflammation
      • as early as 2 months of age and increasing in severity with age   (MGI Ref ID J:93916)
  • vision/eye phenotype
  • abnormal eye morphology
    • at 6 to 10 months of age, 13 of 102 mice exhibit difficulties in maintaining fully opened eyelids due to skin lesions around the eyes unlike wild-type mice   (MGI Ref ID J:93916)
    • after 1 year, 35 of 41 mice exhibit eye abnormalities compared with wild-type mice   (MGI Ref ID J:93916)
    • abnormal lacrimal gland morphology
      • aged mice exhibit destruction of lacrimal gland tissue with opaque spots unlike wild-type mice   (MGI Ref ID J:93916)
  • abnormal lacrimal gland physiology
    • as early as 2 to 4 months of age, mice exhibit reduced tear production compared with wild-type mice   (MGI Ref ID J:93916)
    • however, thymectomy rescues defect   (MGI Ref ID J:93916)
    • lacrimal gland inflammation
      • as early as 2 months of age prior to eye abnormalities   (MGI Ref ID J:93916)
  • digestive/alimentary phenotype
  • decreased salivation
    • as early as 2 to 4 months of age   (MGI Ref ID J:93916)
    • however, thymectomy rescues defect   (MGI Ref ID J:93916)
  • salivary gland inflammation
    • as early as 2 months of age and increasing in severity with age   (MGI Ref ID J:93916)
  • homeostasis/metabolism phenotype
  • decreased salivation
    • as early as 2 to 4 months of age   (MGI Ref ID J:93916)
    • however, thymectomy rescues defect   (MGI Ref ID J:93916)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      Sjogren syndrome
Immunodeficiency
      B cell defects
      Sjogren syndrome
      defects in humoral immune responses

Research Tools
Immunology, Inflammation and Autoimmunity Research
      B cell deficiency
      production of B cells and antibodies

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Id3tm1Zhu
Allele Name targeted mutation 1, Yuan Zhuang
Allele Type Targeted (Null/Knockout)
Common Name(s) Id3-;
Mutation Made By Yuan Zhuang,   Duke University Medical Center
Strain of Origin129S4/SvJaeSor
ES Cell Line NameAK7
ES Cell Line Strain129S4/SvJaeSor
Gene Symbol and Name Id3, inhibitor of DNA binding 3
Chromosome 4
Gene Common Name(s) HEIR-1; HLH462; Idb3; bHLHb25;
Molecular Note The first 2 exons and part of exon 3 were replaced by a PGK neomycin cassette. Gene disruption was confirmed by Northern blot analysis. [MGI Ref ID J:56950]

Genotyping

Genotyping Information

Genotyping Protocols

Id3tm1Zhu, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Pan L; Sato S; Frederick JP; Sun XH; Zhuang Y. 1999. Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene. Mol Cell Biol 19(9):5969-80. [PubMed: 10454544]  [MGI Ref ID J:56950]

Additional References

Id3tm1Zhu related

Akerfeldt MC; Laybutt DR. 2011. Inhibition of Id1 augments insulin secretion and protects against high-fat diet-induced glucose intolerance. Diabetes 60(10):2506-14. [PubMed: 21940780]  [MGI Ref ID J:189476]

Bagavant H; Scindia Y; Nackiewicz D; Rao Nandula S; Doran A; Cutchins A; Oldham S; Deshmukh U; McNamara C. 2011. Deficiency of a transcriptional regulator, inhibitor of differentiation 3, induces glomerulonephritis in apolipoprotein e-deficient mice a model linking hyperlipidemia and renal disease. Am J Pathol 179(2):651-60. [PubMed: 21801865]  [MGI Ref ID J:174603]

Bai G; Sheng N; Xie Z; Bian W; Yokota Y; Benezra R; Kageyama R; Guillemot F; Jing N. 2007. Id sustains Hes1 expression to inhibit precocious neurogenesis by releasing negative autoregulation of Hes1. Dev Cell 13(2):283-97. [PubMed: 17681138]  [MGI Ref ID J:163127]

Benezra R; Henke E; Ciarrocchi A; Ruzinova M; Solit D; Rosen N; Nolan D; Mittal V; de Candia P. 2005. Induction of complete regressions of oncogene-induced breast tumors in mice. Cold Spring Harb Symp Quant Biol 70:375-81. [PubMed: 16869774]  [MGI Ref ID J:115183]

Clever JL; Sakai Y; Wang RA; Schneider DB. 2010. Inefficient skeletal muscle repair in inhibitor of differentiation knockout mice suggests a crucial role for BMP signaling during adult muscle regeneration. Am J Physiol Cell Physiol 298(5):C1087-99. [PubMed: 20181926]  [MGI Ref ID J:159249]

Connerney J; Andreeva V; Leshem Y; Muentener C; Mercado MA; Spicer DB. 2006. Twist1 dimer selection regulates cranial suture patterning and fusion. Dev Dyn 235(5):1334-46. [PubMed: 16502419]  [MGI Ref ID J:108218]

Deliri H; Meller N; Kadakkal A; Malhotra R; Brewster J; Doran AC; Pei H; Oldham SN; Skaflen MD; Garmey JC; McNamara CA. 2011. Increased 12/15-lipoxygenase enhances cell growth, fibronectin deposition, and neointimal formation in response to carotid injury. Arterioscler Thromb Vasc Biol 31(1):110-6. [PubMed: 20947825]  [MGI Ref ID J:184196]

Doran AC; Lehtinen AB; Meller N; Lipinski MJ; Slayton RP; Oldham SN; Skaflen MD; Yeboah J; Rich SS; Bowden DW; McNamara CA. 2010. Id3 is a novel atheroprotective factor containing a functionally significant single-nucleotide polymorphism associated with intima-media thickness in humans. Circ Res 106(7):1303-11. [PubMed: 20185798]  [MGI Ref ID J:171034]

Doran AC; Lipinski MJ; Oldham SN; Garmey JC; Campbell KA; Skaflen MD; Cutchins A; Lee DJ; Glover DK; Kelly KA; Galkina EV; Ley K; Witztum JL; Tsimikas S; Bender TP; McNamara CA. 2012. B-cell aortic homing and atheroprotection depend on Id3. Circ Res 110(1):e1-12. [PubMed: 22034493]  [MGI Ref ID J:192716]

Du Y; Yip HK. 2011. The expression and roles of inhibitor of DNA binding helix-loop-helix proteins in the developing and adult mouse retina. Neuroscience 175:367-79. [PubMed: 21145943]  [MGI Ref ID J:170418]

Fraidenraich D; Stillwell E; Romero E; Wilkes D; Manova K; Basson CT; Benezra R. 2004. Rescue of cardiac defects in id knockout embryos by injection of embryonic stem cells. Science 306(5694):247-52. [PubMed: 15472070]  [MGI Ref ID J:93083]

Haks MC; Lefebvre JM; Lauritsen JP; Carleton M; Rhodes M; Miyazaki T; Kappes DJ; Wiest DL. 2005. Attenuation of gammadeltaTCR signaling efficiently diverts thymocytes to the alphabeta lineage. Immunity 22(5):595-606. [PubMed: 15894277]  [MGI Ref ID J:99103]

Hayakawa I; Tedder TF; Zhuang Y. 2007. B-lymphocyte depletion ameliorates Sjogren's syndrome in Id3 knockout mice. Immunology 122(1):73-9. [PubMed: 17472721]  [MGI Ref ID J:125532]

Ji Y; Pos Z; Rao M; Klebanoff CA; Yu Z; Sukumar M; Reger RN; Palmer DC; Borman ZA; Muranski P; Wang E; Schrump DS; Marincola FM; Restifo NP; Gattinoni L. 2011. Repression of the DNA-binding inhibitor Id3 by Blimp-1 limits the formation of memory CD8+ T cells. Nat Immunol 12(12):1230-7. [PubMed: 22057288]  [MGI Ref ID J:179007]

Koltsova EK; Ciofani M; Benezra R; Miyazaki T; Clipstone N; Zuniga-Pflucker JC; Wiest DL. 2007. Early growth response 1 and NF-ATc1 act in concert to promote thymocyte development beyond the beta-selection checkpoint. J Immunol 179(7):4694-703. [PubMed: 17878368]  [MGI Ref ID J:152341]

Kramer JM; Klimatcheva E; Rothstein TL. 2013. CXCL13 is elevated in Sjogren's syndrome in mice and humans and is implicated in disease pathogenesis. J Leukoc Biol 94(5):1079-89. [PubMed: 23904442]  [MGI Ref ID J:209551]

Lasorella A; Benezra R; Iavarone A. 2014. The ID proteins: master regulators of cancer stem cells and tumour aggressiveness. Nat Rev Cancer 14(2):77-91. [PubMed: 24442143]  [MGI Ref ID J:205559]

Lauritsen JP; Wong GW; Lee SY; Lefebvre JM; Ciofani M; Rhodes M; Kappes DJ; Zuniga-Pflucker JC; Wiest DL. 2009. Marked induction of the helix-loop-helix protein Id3 promotes the gammadelta T cell fate and renders their functional maturation Notch independent. Immunity 31(4):565-75. [PubMed: 19833086]  [MGI Ref ID J:153805]

Li H; Dai M; Zhuang Y. 2004. A T cell intrinsic role of Id3 in a mouse model for primary Sjogren's syndrome. Immunity 21(4):551-60. [PubMed: 15485632]  [MGI Ref ID J:93916]

Li H; Gerald WL; Benezra R. 2004. Utilization of bone marrow-derived endothelial cell precursors in spontaneous prostate tumors varies with tumor grade. Cancer Res 64(17):6137-43. [PubMed: 15342397]  [MGI Ref ID J:92409]

Lipinski MJ; Campbell KA; Duong SQ; Welch TJ; Garmey JC; Doran AC; Skaflen MD; Oldham SN; Kelly KA; McNamara CA. 2012. Loss of Id3 increases VCAM-1 expression, macrophage accumulation, and atherogenesis in Ldlr-/- mice. Arterioscler Thromb Vasc Biol 32(12):2855-61. [PubMed: 23042815]  [MGI Ref ID J:208316]

Liu X; Chen X; Zhong B; Wang A; Wang X; Chu F; Nurieva RI; Yan X; Chen P; van der Flier LG; Nakatsukasa H; Neelapu SS; Chen W; Clevers H; Tian Q; Qi H; Wei L; Dong C. 2014. Transcription factor achaete-scute homologue 2 initiates follicular T-helper-cell development. Nature 507(7493):513-8. [PubMed: 24463518]  [MGI Ref ID J:208784]

Lyden D; Young AZ; Zagzag D; Yan W; Gerald W; O'Reilly R; Bader BL; Hynes RO; Zhuang Y; Manova K; Benezra R. 1999. Id1 and Id3 are required for neurogenesis, angiogenesis and vascularization of tumour xenografts. Nature 401(6754):670-7. [PubMed: 10537105]  [MGI Ref ID J:86480]

Mahlios J; Zhuang Y. 2011. Contribution of IL-13 to early exocrinopathy in Id3(-/-) mice. Mol Immunol 49(1-2):227-33. [PubMed: 21924496]  [MGI Ref ID J:177372]

Maruyama T; Li J; Vaque JP; Konkel JE; Wang W; Zhang B; Zhang P; Zamarron BF; Yu D; Wu Y; Zhuang Y; Gutkind JS; Chen W. 2011. Control of the differentiation of regulatory T cells and T(H)17 cells by the DNA-binding inhibitor Id3. Nat Immunol 12(1):86-95. [PubMed: 21131965]  [MGI Ref ID J:167447]

Miller R; Cirulli V; Diaferia GR; Ninniri S; Hardiman G; Torbett BE; Benezra R; Crisa L. 2008. Switching-on survival and repair response programs in islet transplants by bone marrow-derived vasculogenic cells. Diabetes 57(9):2402-12. [PubMed: 18519801]  [MGI Ref ID J:141799]

Niola F; Zhao X; Singh D; Castano A; Sullivan R; Lauria M; Nam HS; Zhuang Y; Benezra R; Di Bernardo D; Iavarone A; Lasorella A. 2012. Id proteins synchronize stemness and anchorage to the niche of neural stem cells. Nat Cell Biol 14(5):477-87. [PubMed: 22522171]  [MGI Ref ID J:185426]

Niola F; Zhao X; Singh D; Sullivan R; Castano A; Verrico A; Zoppoli P; Friedmann-Morvinski D; Sulman E; Barrett L; Zhuang Y; Verma I; Benezra R; Aldape K; Iavarone A; Lasorella A. 2013. Mesenchymal high-grade glioma is maintained by the ID-RAP1 axis. J Clin Invest 123(1):405-17. [PubMed: 23241957]  [MGI Ref ID J:194297]

Owens AP 3rd; Subramanian V; Moorleghen JJ; Guo Z; McNamara CA; Cassis LA; Daugherty A. 2010. Angiotensin II induces a region-specific hyperplasia of the ascending aorta through regulation of inhibitor of differentiation 3. Circ Res 106(3):611-9. [PubMed: 20019328]  [MGI Ref ID J:170884]

Park K; He X; Lee HO; Hua X; Li Y; Wiest D; Kappes DJ. 2010. TCR-mediated ThPOK induction promotes development of mature (CD24-) gammadelta thymocytes. EMBO J 29(14):2329-41. [PubMed: 20551904]  [MGI Ref ID J:162087]

Pereira de Sousa A; Berthault C; Granato A; Dias S; Ramond C; Kee BL; Cumano A; Vieira P. 2012. Inhibitors of DNA binding proteins restrict T cell potential by repressing Notch1 expression in Flt3-negative common lymphoid progenitors. J Immunol 189(8):3822-30. [PubMed: 22972921]  [MGI Ref ID J:190521]

Perry SS; Zhao Y; Nie L; Cochrane SW; Huang Z; Sun XH. 2007. Id1, but not Id3, directs long-term repopulating hematopoietic stem-cell maintenance. Blood 110(7):2351-60. [PubMed: 17622570]  [MGI Ref ID J:144696]

Ruzinova MB; Schoer RA; Gerald W; Egan JE; Pandolfi PP; Rafii S; Manova K; Mittal V; Benezra R. 2003. Effect of angiogenesis inhibition by Id loss and the contribution of bone-marrow-derived endothelial cells in spontaneous murine tumors. Cancer Cell 4(4):277-89. [PubMed: 14585355]  [MGI Ref ID J:87303]

Shaked Y; Ciarrocchi A; Franco M; Lee CR; Man S; Cheung AM; Hicklin DJ; Chaplin D; Foster FS; Benezra R; Kerbel RS. 2006. Therapy-induced acute recruitment of circulating endothelial progenitor cells to tumors. Science 313(5794):1785-7. [PubMed: 16990548]  [MGI Ref ID J:129431]

Shaked Y; Henke E; Roodhart JM; Mancuso P; Langenberg MH; Colleoni M; Daenen LG; Man S; Xu P; Emmenegger U; Tang T; Zhu Z; Witte L; Strieter RM; Bertolini F; Voest EE; Benezra R; Kerbel RS. 2008. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents. Cancer Cell 14(3):263-73. [PubMed: 18772115]  [MGI Ref ID J:141159]

Taylor AM; Hanchett R; Natarajan R; Hedrick CC; Forrest S; Nadler JL; McNamara CA. 2005. The effects of leukocyte-type 12/15-lipoxygenase on Id3-mediated vascular smooth muscle cell growth. Arterioscler Thromb Vasc Biol 25(10):2069-74. [PubMed: 16037566]  [MGI Ref ID J:135379]

Thal MA; Carvalho TL; He T; Kim HG; Gao H; Hagman J; Klug CA. 2009. Ebf1-mediated down-regulation of Id2 and Id3 is essential for specification of the B cell lineage. Proc Natl Acad Sci U S A 106(2):552-7. [PubMed: 19122139]  [MGI Ref ID J:143875]

Ueda-Hayakawa I; Mahlios J; Zhuang Y. 2009. Id3 restricts the developmental potential of gammadelta lineage during thymopoiesis. J Immunol 182(9):5306-16. [PubMed: 19380777]  [MGI Ref ID J:147720]

Verykokakis M; Boos MD; Bendelac A; Kee BL. 2010. SAP protein-dependent natural killer T-like cells regulate the development of CD8(+) T cells with innate lymphocyte characteristics. Immunity 33(2):203-15. [PubMed: 20674402]  [MGI Ref ID J:163924]

Verykokakis M; Krishnamoorthy V; Iavarone A; Lasorella A; Sigvardsson M; Kee BL. 2013. Essential functions for ID proteins at multiple checkpoints in invariant NKT cell development. J Immunol 191(12):5973-83. [PubMed: 24244015]  [MGI Ref ID J:207115]

Yan W; Young AZ; Soares VC; Kelley R; Benezra R; Zhuang Y. 1997. High incidence of T-cell tumors in E2A-null mice and E2A/Id1 double-knockout mice. Mol Cell Biol 17(12):7317-27. [PubMed: 9372963]  [MGI Ref ID J:44278]

Zarin P; Wong GW; Mohtashami M; Wiest DL; Zuniga-Pflucker JC. 2014. Enforcement of gammadelta-lineage commitment by the pre-T-cell receptor in precursors with weak gammadelta-TCR signals. Proc Natl Acad Sci U S A 111(15):5658-63. [PubMed: 24706811]  [MGI Ref ID J:208631]

Zhang B; Lin YY; Dai M; Zhuang Y. 2014. Id3 and Id2 act as a dual safety mechanism in regulating the development and population size of innate-like gammadelta T cells. J Immunol 192(3):1055-63. [PubMed: 24379125]  [MGI Ref ID J:207300]

Zhao Q; Beck AJ; Vitale JM; Schneider JS; Gao S; Chang C; Elson G; Leibovich SJ; Park JY; Tian B; Nam HS; Fraidenraich D. 2011. Developmental ablation of Id1 and Id3 genes in the vasculature leads to postnatal cardiac phenotypes. Dev Biol 349(1):53-64. [PubMed: 20937270]  [MGI Ref ID J:167088]

de Candia P; Solit DB; Giri D; Brogi E; Siegel PM; Olshen AB; Muller WJ; Rosen N; Benezra R. 2003. Angiogenesis impairment in Id-deficient mice cooperates with an Hsp90 inhibitor to completely suppress HER2/neu-dependent breast tumors. Proc Natl Acad Sci U S A 100(21):12337-42. [PubMed: 14526102]  [MGI Ref ID J:86209]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes. Homozygous mice begin to exhibit an autoimmune phenotype at 6 months of age.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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