Strain Name: |
B6;SJL-Slc6a3tm1.1(cre)Bkmn/J |
|---|---|
Stock Number: |
006302 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Slc6a3; Slc6a3tm1.1(cre)Bkmn; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Cristina Backman, National Institute on Drug Abuse (NIH) Generation ?+N3 (07-MAY-08) Strain Description
Mice homozygous for this dopamine transporter IRES-cre (DATIREScre or DAT-cre) mutant allele are viable and fertile. Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lower Cre recombinase activity is detected in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. Although the pattern and intensity of DAT immunostaining in the SN, VTA and striatum do not differ between wildtype and mutant mice, striatum DAT protein levels are moderately reduced (17%) in heterozygotes and significantly reduced (47%) in homozygotes. This decrease in DAT protein levels in homozygous mutant striatum is associated with significantly increased neuropeptide PDyn (but not D1, D2, or PPE) mRNA levels compared to wildtype. Increases in these mRNA levels are not observed in heterozygotes. When these mice are bred with mice containing a loxP-flanked ("floxed") sequence of interest, cre-mediated recombination will result in deletion of the flanked genome in dopaminergic neurons in the cre-positive, homozygous floxed offspring. These mutant mice may facilitate gene function analysis in dopaminergic neurons in neurological studies of, for example, drug addiction or Parkinson's disease.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Slc6a3tm1.1(cre)Bkmn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Cristina M Backman | ||
| Common Name(s) | DatIREScre; | ||
| Mutation Made By | Andreas Tomac, National Institute on Drug Abuse (NIH) | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | Cre recombinase activity is observed as early as embryonic day 15, and co-localizes with endogenous gene expression in adult dopaminergic cell groups (substantia nigra (SN) and ventral tegmental area (VTA), as well as in the retrorubral field). Lesser Cre recombinase activity occurs in adult olfactory bulb glomeruli, mimicking the known lower Slc6a3 (or DAT) expression in this tissue. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Slc6a3, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | DAT; DAT1; Dat1; dopamine transporter 1; | ||
| Molecular Note | An FRT-flanked neo was removed from the locus, leaving cre recombinase cDNA in the 3' UTR to allow bicistronic mRNA expression. C57BL/6 ES cells were also used. [MGI Ref ID J:114466] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | (approximate) | |
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Slc6a3tm1.1(cre)Bkmn
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, heterozygous mice are bred to wildtype siblings or to B6SJLF1/J (Stock No. 100012) F1 hybrids. Homozygous mutant mice are fertile. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (115 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedCell Biology Research
Channel and Transporter Defects
Neurobiology Research
Behavioral and Learning Defects
Channel and Transporter Defects
Cre-lox System (Cre Recombinase expression in neural tissue)
Neurotransmitter Receptor and Synaptic Vesicle Defects
Parkinson's Disease (strains expressing cre)
Research Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Backman CM; Malik N; Zhang Y; Shan L; Grinberg A; Hoffer BJ; Westphal H; Tomac AC. 2006. Characterization of a mouse strain expressing Cre recombinase from the 3' untranslated region of the dopamine transporter locus. Genesis 44(8):383-90. [PubMed: 16865686] [MGI Ref ID J:114466]
Price and Supply Information
| Strain Name: | B6;SJL-Slc6a3tm1.1(cre)Bkmn/J |
| Stock Number: | 006302 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form