Strain Name:

B6Ei.Cg-Nr0b1tm1.1Lja Tg(Sry)2Ei Chr YAKR/EiJ

Stock Number:

006305

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Consomic;
Additional information on Consomic Mice.
Type Congenic; Mutant Strain; Targeted Mutation; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN10F?pN1
Generation Definitions
 
Donating Investigator Eva Eicher,   The Jackson Laboratory

Related Strains

Strains carrying   Nr0b1tm1.1Lja allele
007007   D2.129(B6)-Nr0b1tm1.1Lja/EiJ
View Strains carrying   Nr0b1tm1.1Lja     (1 strain)

Strains carrying   Tg(Sry)2Ei allele
010905   B6.Cg-Tg(Sry)2Ei Srydl1Rlb/ArnoJ
002708   C57BL/6JEi-Tg(Sry)2Ei Chr YAKR/J/EiJ
View Strains carrying   Tg(Sry)2Ei     (2 strains)

Strains carrying other alleles of Nr0b1
007006   B6Ei.129-Nr0b1tm1Lja/EiJ
View Strains carrying other alleles of Nr0b1     (1 strain)

View Strains carrying other alleles of Sry     (11 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
46,xy Sex Reversal 2; SRXY2   (NR0B1)
Adrenal Hypoplasia, Congenital; AHC   (NR0B1)
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
46,xx Sex Reversal 1; SRXX1   (SRY)
46,xy Sex Reversal 1; SRXY1   (SRY)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tg(Sry)2Ei related

Developmental Biology Research
Internal/Organ Defects
      gonads

Reproductive Biology Research
Developmental Defects Affecting Gonads

Research Tools
Developmental Biology Research
Reproductive Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Nr0b1tm1.1Lja
Allele Name targeted mutation 1.1, J Larry Jameson
Allele Type Targeted (knock-out)
Common Name(s) Ahchdelta2; Dax1-; Dax1KO;
Mutation Made By Linda Washburn,   The Jackson Laboratory
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Nr0b1, nuclear receptor subfamily 0, group B, member 1
Chromosome X
Gene Common Name(s) AHC; AHCH; AHX; Ahc; Ahch; DAX-1; DAX1; DSS; GTD; HHG; NROB1; SRXY2; adrenal hypoplasia, congenital; adrenal hypoplasia, congenital homolog (human);
Molecular Note This allele is a derivative of Nr0b1tm1Lja in which Cre-mediated recombination in vivo under control of a CMV promoter excised exon 2 in the germline. RT-PCR analyses of pituitary, adrenal glands, and gonads did not detect full length transcript in either homozygous female mice or hemizygous male mice. [MGI Ref ID J:51292]
 
Allele Symbol Tg(Sry)2Ei
Allele Name transgene insertion 2, Eva M Eicher
Allele Type Transgenic (random, expressed)
Common Name(s) Tg(Sry-129)2Ei;
Strain of OriginC57BL/6JEi-Chr Y
Expressed Gene Sry, sex determining region of Chr Y, mouse, laboratory
Promoter Sry, sex determining region of Chr Y, mouse, laboratory
Molecular Note This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. [MGI Ref ID J:71077]
 

Genotyping

Genotyping Information

Genotyping Protocols

Nr0b1tm1Lja, tm1.1Lja, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Nr0b1tm1.1Lja related

Achermann JC; Meeks JJ; Larry Jameson J. 2001. Phenotypic spectrum of mutations in DAX-1 and SF-1. Mol Cell Endocrinol 185(1-2):17-25. [PubMed: 11738790]  [MGI Ref ID J:73634]

Babu PS; Bavers DL; Beuschlein F; Shah S; Jeffs B; Jameson JL; Hammer GD. 2002. Interaction between Dax-1 and steroidogenic factor-1 in vivo: increased adrenal responsiveness to ACTH in the absence of Dax-1. Endocrinology 143(2):665-73. [PubMed: 11796523]  [MGI Ref ID J:74975]

Bouma GJ; Albrecht KH; Washburn LL; Recknagel AK; Churchill GA; Eicher EM. 2005. Gonadal sex reversal in mutant Dax1 XY mice: a failure to upregulate Sox9 in pre-Sertoli cells. Development 132(13):3045-54. [PubMed: 15944188]  [MGI Ref ID J:98922]

Brennan J; Capel B. 2004. One tissue, two fates: molecular genetic events that underlie testis versus ovary development. Nat Rev Genet 5(7):509-21. [PubMed: 15211353]  [MGI Ref ID J:90770]

Jeffs B; Ito M; Yu RN; Martinson FA; Wang ZJ; Doglio LT; Jameson JL. 2001. Sertoli cell-specific rescue of fertility, but not testicular pathology, in Dax1 (Ahch)-deficient male mice. Endocrinology 142(6):2481-8. [PubMed: 11356697]  [MGI Ref ID J:70206]

Jeffs B; Meeks JJ; Ito M; Martinson FA; Matzuk MM; Jameson JL; Russell LD. 2001. Blockage of the rete testis and efferent ductules by ectopic sertoli and leydig cells causes infertility in dax1-deficient male mice. Endocrinology 142(10):4486-95. [PubMed: 11564714]  [MGI Ref ID J:71710]

Kopp P. 1999. Targeted disruption of the Ahch (Dax-1) gene: knockout of old concepts. Eur J Endocrinol 140(4):291-2. [PubMed: 10097246]  [MGI Ref ID J:54550]

Martin LJ; Tremblay JJ. 2010. Nuclear receptors in leydig cell gene expression and function. Biol Reprod 83(1):3-14. [PubMed: 20375256]  [MGI Ref ID J:161974]

Meeks JJ; Crawford SE; Russell TA; Morohashi K; Weiss J; Jameson JL. 2003. Dax1 regulates testis cord organization during gonadal differentiation. Development 130(5):1029-36. [PubMed: 12538527]  [MGI Ref ID J:81356]

Meeks JJ; Russell TA; Jeffs B; Huhtaniemi I; Weiss J; Jameson JL. 2003. Leydig cell-specific expression of DAX1 improves fertility of the Dax1-deficient mouse. Biol Reprod 69(1):154-60. [PubMed: 12606353]  [MGI Ref ID J:84030]

Meeks JJ; Weiss J; Jameson JL. 2003. Dax1 is required for testis determination. Nat Genet 34(1):32-3. [PubMed: 12679814]  [MGI Ref ID J:83187]

Park SY; Lee EJ; Emge D; Jahn CL; Jameson JL. 2008. A phenotypic spectrum of sexual development in Dax1 (Nr0b1)-deficient mice: consequence of the C57BL/6J strain on sex determination. Biol Reprod 79(6):1038-45. [PubMed: 18633137]  [MGI Ref ID J:145808]

Park SY; Meeks JJ; Raverot G; Pfaff LE; Weiss J; Hammer GD; Jameson JL. 2005. Nuclear receptors Sf1 and Dax1 function cooperatively to mediate somatic cell differentiation during testis development. Development 132(10):2415-23. [PubMed: 15829514]  [MGI Ref ID J:98517]

Scheys JO; Heaton JH; Hammer GD. 2011. Evidence of adrenal failure in aging Dax1-deficient mice. Endocrinology 152(9):3430-9. [PubMed: 21733829]  [MGI Ref ID J:176895]

Wang ZJ; Jeffs B; Ito M; Achermann JC; Yu RN; Hales DB; Jameson JL. 2001. Aromatase (Cyp19) expression is up-regulated by targeted disruption of Dax1. Proc Natl Acad Sci U S A 98(14):7988-93. [PubMed: 11427738]  [MGI Ref ID J:191932]

Yu RN; Ito M; Saunders TL; Camper SA; Jameson JL. 1998. Role of Ahch in gonadal development and gametogenesis [see comments] Nat Genet 20(4):353-7. [PubMed: 9843206]  [MGI Ref ID J:51292]

Tg(Sry)2Ei related

Albrecht KH; Young M; Washburn LL; Eicher EM. 2003. Sry expression level and protein isoform differences play a role in abnormal testis development in C57BL/6J mice carrying certain Sry alleles. Genetics 164(1):277-88. [PubMed: 12750339]  [MGI Ref ID J:127090]

Carruth LL; Reisert I; Arnold AP. 2002. Sex chromosome genes directly affect brain sexual differentiation. Nat Neurosci 5(10):933-4. [PubMed: 12244322]  [MGI Ref ID J:130009]

Gatewood JD; Wills A; Shetty S; Xu J; Arnold AP; Burgoyne PS; Rissman EF. 2006. Sex chromosome complement and gonadal sex influence aggressive and parental behaviors in mice. J Neurosci 26(8):2335-42. [PubMed: 16495461]  [MGI Ref ID J:105767]

Mahadevaiah SK; Odorisio T; Elliott DJ; Rattigan A; Szot M; Laval SH ; Washburn LL ; McCarrey JR ; Cattanach BM ; Lovell-Badge R ; Burgoyne PS. 1998. Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum Mol Genet 7(4):715-27. [PubMed: 9499427]  [MGI Ref ID J:46791]

Quinn JJ; Hitchcott PK; Umeda EA; Arnold AP; Taylor JR. 2007. Sex chromosome complement regulates habit formation. Nat Neurosci 10(11):1398-400. [PubMed: 17952068]  [MGI Ref ID J:128462]

Smith-Bouvier DL; Divekar AA; Sasidhar M; Du S; Tiwari-Woodruff SK; King JK; Arnold AP; Singh RR; Voskuhl RR. 2008. A role for sex chromosome complement in the female bias in autoimmune disease. J Exp Med 205(5):1099-108. [PubMed: 18443225]  [MGI Ref ID J:136240]

Szot M; Grigoriev V; Mahadevaiah SK; Ojarikre OA; Toure A; von Glasenapp E; Rattigan A; Turner JM; Elliott DJ; Burgoyne PS. 2003. Does Rbmy have a role in sperm development in mice? Cytogenet Genome Res 103(3-4):330-6. [PubMed: 15051956]  [MGI Ref ID J:89312]

Toure A; Szot M; Mahadevaiah SK; Rattigan A; Ojarikre OA; Burgoyne PS. 2004. A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility. Genetics 166(2):901-12. [PubMed: 15020475]  [MGI Ref ID J:89616]

Wagner CK; Xu J; Pfau JL; Quadros PS; De Vries GJ; Arnold AP. 2004. Neonatal mice possessing an Sry transgene show a masculinized pattern of progesterone receptor expression in the brain independent of sex chromosome status. Endocrinology 145(3):1046-9. [PubMed: 14645115]  [MGI Ref ID J:88702]

Ward MA; Burgoyne PS. 2006. The Effects of Deletions of the Mouse Y Chromosome Long Arm on Sperm Function--Intracytoplasmic Sperm Injection (ICSI)-Based Analysis. Biol Reprod 74(4):652-8. [PubMed: 16354792]  [MGI Ref ID J:107012]

Washburn LL; Albrecht KH; Eicher EM. 2001. C57BL/6J-T-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a Mus domesticus Sry Allele. Genetics 158(4):1675-81. [PubMed: 11514455]  [MGI Ref ID J:71077]

Yamauchi Y; Riel JM; Stoytcheva Z; Burgoyne PS; Ward MA. 2010. Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage. Genome Biol 11(6):R66. [PubMed: 20573212]  [MGI Ref ID J:179194]

Yamauchi Y; Riel JM; Stoytcheva Z; Ward MA. 2014. Two Y genes can replace the entire Y chromosome for assisted reproduction in the mouse. Science 343(6166):69-72. [PubMed: 24263135]  [MGI Ref ID J:205194]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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