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| ROSA26-eGFP-DTA transgenic mice display widespread expression of EGFP, but DTA transcription is prevented by a strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. This strain may be useful on its own as a fluorescent reporter or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. Transgenic mice also may have applications in toxicology and protein synthesis research. | |||||||||||||||
- Description
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Description
Strain Information
Former Names STOCK Gt(ROSA)26Sortm1Jpmb/J (Changed: 19-OCT-06 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Heterozygote (Female x Male) 03-DEC-08 Species laboratory mouse Generation F?+N1F6 (30-OCT-08) Donating Investigator Juan Pedro Martinez-Barbera, UCL Institute of Child Health Description
Homozygous "ROSA26-eGFP-DTA" mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that some homozygous males are subfertile. Mutant mice display widespread expression of EGFP, but DTA transcription is prevented by a strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. This strain may be useful on its own as a fluorescent reporter or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. Transgenic mice also may have applications in toxicology and protein synthesis research.For example, when crossed to a strain expressing Cre recombinase in the midbrain and dorsal spinal cord (see Stock No. 007807), DTA expression is activated resulting in elimination of the cre-expressing cells, this mutant mouse strain may be useful in studies of midbrain and neural tube development.
Development
A targeting vector was designed placing a loxP-flanked Enhanced Green Fluorescent Protein (EGFP, Clontech), PGK-Neo cassette, and a triple SV40 polyadenylation signal fragment upstream of the diphtheria toxin A subunit (DTA). This construct was inserted into the Gt(ROSA)26Sor locus via electroporation into 129S6/SvEvTac-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were bred to C57BL/6J females. The resulting heterozygotes were bred to CD1 mice for 2-3 generations and then made homozygous prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Gt(ROSA)26Sortm1(DTA)Jpmb/Gt(ROSA)26Sortm1(DTA)Jpmb
either: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * CD-1)
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:103417)
- homozygotes are viable and fertile with no gross abnormalities
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gt(ROSA)26Sortm1(DTA)Jpmb/Gt(ROSA)26Sor+ Tg(Wnt1-cre)11Rth/0
either: (involves: 129S/SvEv * C57BL/6 * CBA) or (involves: 129S/SvEv * C57BL/6 * CBA * CD-1) (conditional)
- nervous system phenotype
- abnormal midbrain development (MGI Ref ID J:103417)
- at E9.5 and E10.5 the brain rostral to the otic vesicle is significantly smaller
- abnormal neural tube morphology/development (MGI Ref ID J:103417)
- at E9.5 and E10.5, the isthmus (the constriction of the neural tube at the midbrain hindbrain boundary is absent
- open neural tube (MGI Ref ID J:103417)
- at E9.5 and E10.5, the cranial neural tube is open dorsally
- embryogenesis phenotype
- abnormal neural tube morphology/development (MGI Ref ID J:103417)
- at E9.5 and E10.5, the isthmus (the constriction of the neural tube at the midbrain hindbrain boundary is absent
- open neural tube (MGI Ref ID J:103417)
- at E9.5 and E10.5, the cranial neural tube is open dorsally
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Protein Processing
Neurobiology Research
Cre-lox System
loxP-flanked Sequences
Research Tools
Cardiovascular Research
Cre-lox System
Cre-lox System
loxP-flanked Sequences
Developmental Biology Research
Cre-lox System
Diabetes and Obesity Research
loxP
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Reproductive Biology Research
Cre-lox System
Toxicology Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(DTA)Jpmb | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Juan Pedro Martinez-Barbera | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R26DTA; ROSA26eGFP-DTA; ROSA26GFP-DTA; | ||
| Mutation Made By | Juan Pedro Martinez-Barbera, UCL Institute of Child Health | ||
| Strain of Origin | 129S/SvEv-Gpi1 | ||
| ES Cell Line Name | CCE/EK.CCE | ||
| ES Cell Line Strain | 129S/SvEv-Gpi1 | ||
| Site of Expression | Expression of EGFP is widespread. When bred to Cre recombinase expressing mice, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| General Note | The time lapse between onset of Cre expression and cell death is estimated to be less than 24 hours. | ||
| Molecular Note | A targeting vector contained a conditionally expressed DTA construct downstream of a floxed DNA region containing EGFP, a neo cassette and a triple SV40. An EGFP is upstream of the DTA and is constitutively expressed under the control of the ROSA26 protmoter. [MGI Ref ID J:103417] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1sor STD, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ivanova A; Signore M; Caro N; Greene ND; Copp AJ; Martinez-Barbera JP. 2005. In vivo genetic ablation by Cre-mediated expression of diphtheria toxin fragment A. Genesis 43(3):129-35. [PubMed: 16267821] [MGI Ref ID J:103417]
Additional References
Gt(ROSA)26Sortm1(DTA)Jpmb relatedAbrahamsen B; Zhao J; Asante CO; Cendan CM; Marsh S; Martinez-Barbera JP; Nassar MA; Dickenson AH; Wood JN. 2008. The cell and molecular basis of mechanical, cold, and inflammatory pain. Science 321(5889):702-5. [PubMed: 18669863] [MGI Ref ID J:139511]
Snider P; Fix JL; Rogers R; Peabody-Dowling G; Ingram D; Lilly B; Conway SJ. 2008. Generation and characterization of Csrp1 enhancer-driven tissue-restricted Cre-recombinase mice. Genesis 46(3):167-76. [PubMed: 18327771] [MGI Ref ID J:133342]
Snider P; Tang S; Lin G; Wang J; Conway SJ. 2009. Generation of Smad7(-Cre) recombinase mice: A useful tool for the study of epithelial-mesenchymal transformation within the embryonic heart. Genesis 47(7):469-75. [PubMed: 19415626] [MGI Ref ID J:150864]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony at The Jackson Laboratory, homozygous females are bred with heterozygous males. The donating investigator breeds homozygous females with heterozygous males and reports that some homozygous males may be subfertile, although breeding homozygous mice together has been successful at The Jackson Laboratory in the past.
Mating System Homozygote x Heterozygote (Female x Male) 03-DEC-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Gt(ROSA)26Sortm1(DTA)Jpmb $300.70 Female Homozygous for Gt(ROSA)26Sortm1(DTA)Jpmb
Pairs /Price (US dollars $) Pair Genotype $544.20 Homozygous for Gt(ROSA)26Sortm1(DTA)Jpmb x Heterozygous for Gt(ROSA)26Sortm1(DTA)Jpmb
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Gt(ROSA)26Sortm1(DTA)Jpmb $391.00 Female Homozygous for Gt(ROSA)26Sortm1(DTA)Jpmb
Pairs /Price (US dollars $) Pair Genotype $707.50 Homozygous for Gt(ROSA)26Sortm1(DTA)Jpmb x Heterozygous for Gt(ROSA)26Sortm1(DTA)Jpmb
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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