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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.FVB(CD1)-Tg(Neurog3-cre)C1Able/J (Changed: 09-MAY-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 08-JUN-08 Species laboratory mouse Generation N5F8 (27-AUG-10)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Expression of the transgene is directed by a neurogenin 3 promoter. Tissues where Cre recombinase expression is detected include the small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells. Cre activity has been shown in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A bacterial artificial chromosome (BAC) encoding the mouse neurogenin 3 sequence was modified by the insertion of a cre recombinase gene, preceded by nuclear localization sequence, into exon 1 of the neurogenin 3 gene at the initiator methionine. The BAC was injected into FVB/N embryos which were next implanted into pseudopregnant CD1 females. Founder line C1 was obtained and backcrossed to CD1 mice for 7 generations after which mice were intercrossed. The mice were then backcrossed to C57BL/6J for 5 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Neurog3-cre)C1Able allele
005667 STOCK Tg(Neurog3-cre)C1Able/J View Strains carrying Tg(Neurog3-cre)C1Able (1 strain)
005667 STOCK Tg(Neurog3-cre)C1Able/J
005667 STOCK Tg(Neurog3-cre)C1Able/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedEndocrine Deficiency Research
Pancreas Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Endocrine Deficiency Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Neurog3-cre)C1Able | ||
|---|---|---|---|
| Allele Name | transgene insertion C1, Andrew B Leiter | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Neurog3(cre); Tg(Neurog3-cre)#Able; ngn3-Cre; | ||
| Mutation Made By | Andrew Leiter, Univ. of Massachusetts Medical School | ||
| Site of Expression | small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Neurog3, neurogenin 3, rat | ||
| Gene Symbol and Name | Tg(Neurog3-cre)C1Able, transgene insertion C1, Andrew B Leiter | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Neurog3(cre); Tg(Neurog3-cre)#Able; ngn3-Cre; transgene insertion #, Andrew B Leiter; | ||
| Driver Note | Neurog3 | ||
| General Note | This is one of two independent lines demonstrating identical expression patterns. | ||
| Molecular Note | The Cre recombinase coding sequence, flanked by a nuclear localization signal and an SV40 polyadenylation site, was inserted just after the translation start site of the neurogenin 3 gene present in mouse BAC RP23-121F10, which also includes approximately 81 kb of 5' and 102 kb of 3' flanking DNA. Immunohistochemical analysis shows the same expression pattern for cre as for neurogenin 3 in adult and embryonic mice bearing this transgene. Cre recombinase activity is seen in cell types of pancreas, stomach, small intestine and colon that express or are descended from cells that express neurogenin 3 during development. [MGI Ref ID J:92204] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Schonhoff SE; Giel-Moloney M; Leiter AB. 2004. Neurogenin 3-expressing progenitor cells in the gastrointestinal tract differentiate into both endocrine and non-endocrine cell types. Dev Biol 270(2):443-54. [PubMed: 15183725] [MGI Ref ID J:92204]
Additional References
Tg(Neurog3-cre)C1Able relatedCotta-de-Almeida V; Schonhoff S; Shibata T; Leiter A; Snapper SB. 2003. A new method for rapidly generating gene-targeting vectors by engineering BACs through homologous recombination in bacteria. Genome Res 13(9):2190-4. [PubMed: 12915491] [MGI Ref ID J:99831]
Danilova OV; Tai AK; Mele DA; Beinborn M; Leiter AB; Greenberg AS; Perfield JW 2nd; Defuria J; Singru PS; Lechan RM; Huber BT. 2009. Neurogenin 3-specific dipeptidyl peptidase-2 deficiency causes impaired glucose tolerance, insulin resistance, and visceral obesity. Endocrinology 150(12):5240-8. [PubMed: 19819973] [MGI Ref ID J:158215]
Datsenko KA; Wanner BL. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97(12):6640-5. [PubMed: 10829079] [MGI Ref ID J:99832]
Kitamura T; Kitamura YI; Kobayashi M; Kikuchi O; Sasaki T; Depinho RA; Accili D. 2009. Regulation of pancreatic juxtaductal endocrine cell formation by FoxO1. Mol Cell Biol 29(16):4417-30. [PubMed: 19506018] [MGI Ref ID J:151416]
Magenheim J; Klein AM; Stanger BZ; Ashery-Padan R; Sosa-Pineda B; Gu G; Dor Y. 2011. Ngn3(+) endocrine progenitor cells control the fate and morphogenesis of pancreatic ductal epithelium. Dev Biol 359(1):26-36. [PubMed: 21888903] [MGI Ref ID J:178171]
Mastracci TL; Wilcox CL; Arnes L; Panea C; Golden JA; May CL; Sussel L. 2011. Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell development and endocrine hormone expression. Dev Biol 359(1):1-11. [PubMed: 21856296] [MGI Ref ID J:178173]
Pelling M; Anthwal N; McNay D; Gradwohl G; Leiter AB; Guillemot F; Ang SL. 2011. Differential requirements for neurogenin 3 in the development of POMC and NPY neurons in the hypothalamus. Dev Biol 349(2):406-16. [PubMed: 21074524] [MGI Ref ID J:168022]
Serafimidis I; Heximer S; Beis D; Gavalas A. 2011. G protein-coupled receptor signaling and sphingosine-1-phosphate play a phylogenetically conserved role in endocrine pancreas morphogenesis. Mol Cell Biol 31(22):4442-53. [PubMed: 21911471] [MGI Ref ID J:178647]
Song J; Xu Y; Hu X; Choi B; Tong Q. 2010. Brain expression of Cre recombinase driven by pancreas-specific promoters. Genesis 48(11):628-34. [PubMed: 20824628] [MGI Ref ID J:166612]
Wang S; Yan J; Anderson DA; Xu Y; Kanal MC; Cao Z; Wright CV; Gu G. 2010. Neurog3 gene dosage regulates allocation of endocrine and exocrine cell fates in the developing mouse pancreas. Dev Biol 339(1):26-37. [PubMed: 20025861] [MGI Ref ID J:157959]
Wang Y; Giel-Moloney M; Rindi G; Leiter AB. 2007. Enteroendocrine precursors differentiate independently of Wnt and form serotonin expressing adenomas in response to active beta-catenin. Proc Natl Acad Sci U S A 104(27):11328-33. [PubMed: 17592150] [MGI Ref ID J:122831]
Wang Y; Ray SK; Hinds PW; Leiter AB. 2007. The retinoblastoma protein, RB, is required for gastrointestinal endocrine cells to exit the cell cycle, but not for hormone expression. Dev Biol 311(2):478-86. [PubMed: 17936268] [MGI Ref ID J:127522]
Yang YP; Thorel F; Boyer DF; Herrera PL; Wright CV. 2011. Context-specific {alpha}-to-{beta}-cell reprogramming by forced Pdx1 expression. Genes Dev 25(16):1680-5. [PubMed: 21852533] [MGI Ref ID J:174673]
Zhang H; Ables ET; Pope CF; Washington MK; Hipkens S; Means AL; Path G; Seufert J; Costa RH; Leiter AB; Magnuson MA; Gannon M. 2009. Multiple, temporal-specific roles for HNF6 in pancreatic endocrine and ductal differentiation. Mech Dev 126(11-12):958-73. [PubMed: 19766716] [MGI Ref ID J:155058]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained as hemizygotes. Mating System +/+ sibling x Hemizygote (Female x Male) 08-JUN-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Neurog3-cre)C1Able
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Neurog3-cre)C1Able x Noncarrier Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Neurog3-cre)C1Able
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Neurog3-cre)C1Able x Noncarrier Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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