Strain Name: |
B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J |
|---|---|
Stock Number: |
006333 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6.FVB(CD1)-Tg(Neurog3-cre)C1Able/J (Changed: 09-MAY-07
) |
| Genes & Alleles | Neurog3; cre; Tg(Neurog3-cre)C1Able; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N5F1 (24-JUL-07) Strain Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Expression of the transgene is directed by a neurogenin 3 promoter. Tissues where Cre recombinase expression is detected include the small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells. Cre activity has been shown in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A bacterial artificial chromosome (BAC) encoding the mouse neurogenin 3 sequence was modified by the insertion of a cre recombinase gene, preceded by nuclear localization sequence, into exon 1 of the neurogenin 3 gene at the initiator methionine. The BAC was injected into FVB/N embryos which were next implanted into pseudopregnant CD1 females. Founder line C1 was obtained and backcrossed to CD1 mice for 7 generations after which mice were intercrossed. The mice were then backcrossed to C57BL/6J for 5 generations.
Gene & Allele Details
| Allele Symbol | Tg(Neurog3-cre)C1Able | ||
|---|---|---|---|
| Allele Name | transgene insertion C1, Andrew B Leiter | ||
| Common Name(s) | Tg(Neurog3-cre)#Able; ngn3-Cre; | ||
| Mutation Made By | Andrew Leiter, Univ. of Massachusetts Medical School | ||
| Site of Expression | small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Neurog3, neurogenin 3, rat | ||
| General Note | This is one of two independent lines demonstrating identical expression patterns. | ||
| Molecular Note | The Cre recombinase coding sequence, flanked by a nuclear localization signal and an SV40 polyadenylation site, was inserted just after the translation start site of the neurogenin 3 gene present in mouse BAC RP23-121F10, which also includes approximately 81 kb of 5' and 102 kb of 3' flanking DNA. Immunohistochemical analysis shows the same expression pattern for cre as for neurogenin 3 in adult and embryonic mice bearing this transgene. Cre recombinase activity is seen in cell types of pancreas, stomach, small intestine and colon that express or are descended from cells that express neurogenin 3 during development. [MGI Ref ID J:92204] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are maintained as hemizygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Tg(Neurog3-cre)C1Able allele
005667 STOCK Tg(Neurog3-cre)C1Able/J View Strains carrying Tg(Neurog3-cre)C1Able (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedEndocrine Deficiency Research
Pancreas Defects
Research Tools
Cre-lox System (Cre Recombinase Expression)
Endocrine Deficiency Research
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesSchonhoff SE; Giel-Moloney M; Leiter AB. 2004. Neurogenin 3-expressing progenitor cells in the gastrointestinal tract differentiate into both endocrine and non-endocrine cell types. Dev Biol 270(2):443-54. [PubMed: 15183725] [MGI Ref ID J:92204]
Price and Supply Information
| Strain Name: | B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J |
| Stock Number: | 006333 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(Neurog3-cre)C1Able | |||
| Pair | $288.65 | Hemizygous for Tg(Neurog3-cre)C1Able x Noncarrier | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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