| |||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Former Names STOCK Tg(Gad1/EGFP)94Agmo/J (Changed: 14-DEC-07 ) Type Mutant Stock; Transgenic; Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Generation ?+F1 (28-DEC-07) Donating Investigator Ariel Agmon, West Virginia Univ School of Medicine Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the mouse Gad1 (glutamic acid decarboxylase 1 or GAD67) promoter. In the forebrain, EGFP expression is restricted to the somatosensory barrel neocortex, mostly in laminar layers 4 and 5B, to a lesser extent in layers 2/3, 5A and upper 6. EGFP expression is also found in subsets of cerebellar interneurons and in the brainstem. In founder line 94 transgenic mice fluorescence is observed in neocortical neurons with axonal arborizations in layer 4 that have a stuttering firing pattern. Fluorescence levels are low in animals younger than 2 weeks of age. The fluorescently labeled subset of somatostatin-containing (SOM+) interneurons in founder line 94 transgenic mice is distinct from the population of neurons labeled in founder line 98 (Stock No. 006340) or in mice expressing Tg(GadGFP)45704Swn (Stock No. 003718) in location, neurochemical markers, axonal morphologies, and electrophysiological characteristics. This mutant mouse strain may be useful in studies of populations of inhibitory interneurons of the neocortex and cerebellum.Development
A transgenic construct containing EGFP gene under the control of the mouse Gad1, glutamic acid decarboxylase 1, promoter was injected into fertilized C57BL6 X SJL mouse eggs. Founder line 94 was established. Animals were initially bred to C57BL/6 mice and then bred to outbred CD-1 mice. The mice were then bred to outbred Hilltop Swiss ICR mice. The Donating Investigator maintained the strain by mating hemizygotes to outbred mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (155 strains)
007677 CB6-Tg(Gad1-EGFP)G42Zjh/J 003718 FVB-Tg(GadGFP)45704Swn/J 006340 STOCK Tg(Gad1-EGFP)98Agmo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
Tg(Gad1-EGFP)94Agmo/0
involves: C57BL/6 * CD-1 * SJL
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:117601)
- mice are viable and fertile and show no obvious phenotypic abnormalities
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Gad1-EGFP)94Agmo | ||
|---|---|---|---|
| Allele Name | transgene insertion 94, Ariel Agmon | ||
| Common Name(s) | GAD67-GFP; Tg(Gad1/EGFP)94Agmo; X94; | ||
| Mutation Made By | Ariel Agmon, West Virginia Univ School of Medicine | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | forebrain (restricted to the somatosensory barrel neocortex, mostly in laminar layers 4 and 5B, to a lesser extent in layers 2/3, 5A and upper 6); subsets of cerebellar interneurons and in the brainstem; neocortical neurons with axonal arborizations in layer 4 that have a stuttering firing pattern; low fluorescence in animals younger than 2 weeks of age | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gad1, glutamic acid decarboxylase 1, mouse, laboratory | ||
| General Note | Of 11 initial lines derived from founders shown by PCR analysis to carry the transgene, 5 that expressed EGFP in the brain continued to be maintained. Two of these, designated by the authors X94 and X98, in which cortical EGFP expression was limited to nonpyramidal cells were subjected to further analysis. | ||
| Molecular Note | The transgene comprises 10.3 kb of mouse genomic DNA containing the promoter region from the glutamic acid decarboxylase 1 (Gad1, commonly called GAD67) gene, including the 5' regulatory sequences, (noncoding) exon 1, intron 1 and a segment of exon 2, joined in-frame to the coding sequence of enhanced green fluorescent protein followed by an SV40 polyadenylation signal. The encoded fusion protein contains the first 17 amino acids of GAD1, 4 amino acids derived from the pEGFP-1 vector, and the entire enhanced green fluorescent protein. GFP expression is observed in stuttering interneurons with quasi fast-spiking properties in the cerebral cortex. [MGI Ref ID J:117601] [MGI Ref ID J:117729] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Gad1-EGFP)94Agmo, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Ma Y; Hu H; Berrebi AS; Mathers PH; Agmon A. 2006. Distinct subtypes of somatostatin-containing neocortical interneurons revealed in transgenic mice. J Neurosci 26(19):5069-82. [PubMed: 16687498] [MGI Ref ID J:117601]
Additional References
Tg(Gad1-EGFP)94Agmo related
Jin X; Mathers PH; Szabo G; Katarova Z; Agmon A. 2001. Vertical bias in dendritic trees of non-pyramidal neocortical neurons expressing GAD67-GFP in vitro. Cereb Cortex 11(7):666-78. [PubMed: 11415968] [MGI Ref ID J:117729]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Gad1-EGFP)94Agmo *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Gad1-EGFP)94Agmo x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Gad1-EGFP)94Agmo
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Gad1-EGFP)94Agmo *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Gad1-EGFP)94Agmo x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Gad1-EGFP)94Agmo
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form