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Genes & Alleles

Mgat5;   Mgat5tm1Jwd;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator James Dennis,   Samuel Lunenfeld Research Institute

Strain Description
No gene product (protein) is detected by Western blot analysis and enzyme activity is undetectable. Beta-galactosidase activity mimics endogenous gene expression patterns. Homozygotes exhibit abnormal increased leukocyte infiltration in kidney tissue, which is indicative of kidney autoimmune glomerulonephritis. Induced experimental autoimmune encephalomyelitis (EAE) and induced delayed-type hypersensitivity (DTH) responses are increased in homozygotes. Cultured splenocytes isolated from homozygotes produce 2 fold more IFN-gamma and 2 fold less IL4. Mutant mice exhibit delayed tumor progression when bred with oncomice (for example, when crossed to a polyomavirus middle T antigen expressing transgenic strain). Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygous females are not fertile. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, autoimmunity and antitumor immunity.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Strain Development
A targeting vector containing neomycin resistance and beta-galactosidase genes was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting male chimeric animals were crossed to 129 female mice, and then backcrossed to C57BL/6 for 9 generations before arriving at The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Mgat5tm1Jwd/Mgat5tm1Jwd         involves: 129S1/Sv * 129X1/SvJ immune system phenotype abnormal inflammatory response (MGI Ref ID J:60960) abnormal recruitment of leukocytes into inflamed tissue decreased interleukin-2 secretion (MGI Ref ID J:94864) Il2 production is reduced decreased interleukin-4 secretion (MGI Ref ID J:94864) Il4 production is reduced about 2 fold per cell increased interleukin-10 secretion (MGI Ref ID J:94864) production is increased increased tumor necrosis factor secretion (MGI Ref ID J:94864) TNF-alpha production is increased more than 2-fold abnormal leukocyte physiology (MGI Ref ID J:94864) increased splenocyte proliferation after stimulation abnormal T cell physiology (MGI Ref ID J:60960) T cells hypersensitive to T cell receptor agonists tumorigenesis decreased metastatic potential (MGI Ref ID J:60960) lung cancer metastasis about 50% of controls and never causes cardiac hypertrophy decreased tumor growth/size (MGI Ref ID J:60960) tumor growth much slower (3.4g at 28-30 weeks as opposed to 15g in controls) decreased tumor incidence (MGI Ref ID J:60960) 50% reduction in the incidence of mammary tumors at 24 weeks of age rather than at 16 weeks as in controls male tumor development at 10-13 months of age rather than at 6-9 months tumor foci development is normal renal/urinary system phenotype abnormal renal glomerulus morphology (MGI Ref ID J:60960) age related reduction in cellularity of the kidney glomerulus behavior/neurological phenotype abnormal maternal nurturing (MGI Ref ID J:60960) deficiencies in nurturing behavior

Gene & Allele Details

Allele Symbol		Mgat5tm1Jwd
Allele Name		targeted mutation 1, James W Dennis
Common Name(s)		Mgat5-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Mgat5, mannoside acetylglucosaminyltransferase 5
Chromosome		1
Gene Common Name(s)		4930471A21Rik; 5330407H02Rik; AI480971; GNT-V; GlcNAc-TV; RIKEN cDNA 4930471A21 gene; RIKEN cDNA 5330407H02 gene; beta1,6N-acetylglucosaminyltransferase V; expressed sequence AI480971;
Molecular Note		A LacZ and neomycin resistance cassette replaced part of exon 1. [MGI Ref ID J:60960]

Genotyping Protocols

Mgat5^tm1Jwd

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygous females and homozygous males. Homozygous females are infertile.

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Immunology and Inflammation Research
Autoimmunity

References

Selected Reference(s)

Granovsky M; Fata J; Pawling J; Muller WJ; Khokha R; Dennis JW. 2000. Suppression of tumor growth and metastasis in Mgat5-deficient mice. Nat Med 6(3):306-12. [PubMed: 10700233]  [MGI Ref ID J:60960]

Additional References

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Price and Supply Information

Strain Name:	B6.129-Mgat5tm1Jwd/J
Stock Number:	006335

 

This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.

Estimated Available for Sale Date:

Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.

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Supply Details

Standard Supply	Under Development for Distribution Colony
Supply Notes	This strain is included in the Induced Mutant Resource Colony collection.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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