Strain Name:

B6.129-Mgat5tm1Jwd/J

Stock Number:

006335

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
Mgat5 (mannoside acetylglucosaminyltransferase 5) deficient mice exhibit increased responses to induced immune challenges and delayed tumor progression when bred with oncomice. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, autoimmunity and antitumor immunity.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN2F4pN1
Generation Definitions
 
Donating Investigator James W Dennis,   Samuel Lunenfeld Research Institute

Description
No gene product (protein) is detected by Western blot analysis and enzyme activity is undetectable. Beta-galactosidase activity mimics endogenous gene expression patterns. Homozygotes exhibit abnormal increased leukocyte infiltration in kidney tissue, which is indicative of kidney autoimmune glomerulonephritis. Induced experimental autoimmune encephalomyelitis (EAE) and induced delayed-type hypersensitivity (DTH) responses are increased in homozygotes. Cultured splenocytes isolated from homozygotes produce 2 fold more IFN-gamma and 2 fold less IL4. Mutant mice exhibit delayed tumor progression when bred with oncomice (for example, when crossed to a polyomavirus middle T antigen expressing transgenic strain). Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygous females are not fertile. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, autoimmunity and antitumor immunity.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
A targeting vector containing neomycin resistance and beta-galactosidase genes was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting male chimeric animals were crossed to 129 female mice, and then backcrossed to C57BL/6 for 9 generations before arriving at The Jackson Laboratory.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Mgat5tm1Jwd/Mgat5tm1Jwd

        involves: 129S1/Sv * 129X1/SvJ
  • immune system phenotype
  • abnormal inflammatory response
    • abnormal recruitment of leukocytes into inflamed tissue   (MGI Ref ID J:60960)
  • abnormal leukocyte physiology
    • increased splenocyte proliferation after stimulation   (MGI Ref ID J:94864)
    • abnormal T cell physiology
      • T cells hypersensitive to T cell receptor agonists   (MGI Ref ID J:60960)
  • decreased interleukin-2 secretion
    • Il2 production is reduced   (MGI Ref ID J:94864)
  • decreased interleukin-4 secretion
    • Il4 production is reduced about 2 fold per cell   (MGI Ref ID J:94864)
  • increased interleukin-10 secretion
    • production is increased   (MGI Ref ID J:94864)
  • increased tumor necrosis factor secretion
    • TNF-alpha production is increased more than 2-fold   (MGI Ref ID J:94864)
  • tumorigenesis
  • decreased metastatic potential
    • lung cancer metastasis about 50% of controls and never causes cardiac hypertrophy   (MGI Ref ID J:60960)
  • decreased tumor growth/size
    • tumor growth much slower (3.4g at 28-30 weeks as opposed to 15g in controls)   (MGI Ref ID J:60960)
  • increased tumor latency
    • 50% reduction in the incidence of mammary tumors at 24 weeks of age rather than at 16 weeks as in controls   (MGI Ref ID J:60960)
    • male tumor development at 10-13 months of age rather than at 6-9 months   (MGI Ref ID J:60960)
    • tumor foci development is normal   (MGI Ref ID J:60960)
  • renal/urinary system phenotype
  • abnormal renal glomerulus morphology
    • age related reduction in cellularity of the kidney glomerulus   (MGI Ref ID J:60960)
  • behavior/neurological phenotype
  • abnormal maternal nurturing
    • deficiencies in nurturing behavior   (MGI Ref ID J:60960)
  • homeostasis/metabolism phenotype
  • abnormal homeostasis
    • mice exhibit lack N-linked beta1,6-branched glycans   (MGI Ref ID J:188930)
    • however, mice exhibit normal levels of O-man-linked beta1,6-branched glycans   (MGI Ref ID J:188930)
  • hematopoietic system phenotype
  • abnormal leukocyte physiology
    • increased splenocyte proliferation after stimulation   (MGI Ref ID J:94864)
    • abnormal T cell physiology
      • T cells hypersensitive to T cell receptor agonists   (MGI Ref ID J:60960)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Immunology, Inflammation and Autoimmunity Research
Autoimmunity

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Mgat5tm1Jwd
Allele Name targeted mutation 1, James W Dennis
Allele Type Targeted (Reporter)
Common Name(s) GnTV-; Mgat5-;
Mutation Made By Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Mgat5, mannoside acetylglucosaminyltransferase 5
Chromosome 1
Gene Common Name(s) 4930471A21Rik; 5330407H02Rik; AI480971; GNT-V; GNT-VA; GlcNAc-TV; RIKEN cDNA 4930471A21 gene; RIKEN cDNA 5330407H02 gene; beta1,6N-acetylglucosaminyltransferase V; expressed sequence AI480971;
Molecular Note A LacZ and neomycin resistance cassette replaced part of exon 1. [MGI Ref ID J:60960]

Genotyping

Genotyping Information

Genotyping Protocols

Mgat5tm1Jwd, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Granovsky M; Fata J; Pawling J; Muller WJ; Khokha R; Dennis JW. 2000. Suppression of tumor growth and metastasis in Mgat5-deficient mice. Nat Med 6(3):306-12. [PubMed: 10700233]  [MGI Ref ID J:60960]

Additional References

Mgat5tm1Jwd related

Bahaie NS; Kang BN; Frenzel EM; Hosseinkhani MR; Ge XN; Greenberg Y; Ha SG; Demetriou M; Rao SP; Sriramarao P. 2011. N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation. J Biol Chem 286(44):38231-41. [PubMed: 21911487]  [MGI Ref ID J:178166]

Demetriou M; Granovsky M; Quaggin S; Dennis JW. 2001. Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation. Nature 409(6821):733-9. [PubMed: 11217864]  [MGI Ref ID J:111264]

Guo HB; Johnson H; Randolph M; Nagy T; Blalock R; Pierce M. 2010. Specific posttranslational modification regulates early events in mammary carcinoma formation. Proc Natl Acad Sci U S A 107(49):21116-21. [PubMed: 21078982]  [MGI Ref ID J:167163]

Guo HB; Lee I; Bryan BT; Pierce M. 2005. Deletion of mouse embryo fibroblast N-acetylglucosaminyltransferase V stimulates alpha5beta1 integrin expression mediated by the protein kinase C signaling pathway. J Biol Chem 280(9):8332-42. [PubMed: 15615721]  [MGI Ref ID J:97247]

Guo HB; Lee I; Kamar M; Pierce M. 2003. N-acetylglucosaminyltransferase V expression levels regulate cadherin-associated homotypic cell-cell adhesion and intracellular signaling pathways. J Biol Chem 278(52):52412-24. [PubMed: 14561752]  [MGI Ref ID J:87101]

Guo HB; Nairn A; Harris K; Randolph M; Alvarez-Manilla G; Moremen K; Pierce M. 2008. Loss of expression of N-acetylglucosaminyltransferase Va results in altered gene expression of glycosyltransferases and galectins. FEBS Lett 582(4):527-35. [PubMed: 18230362]  [MGI Ref ID J:131841]

Kanekiyo K; Inamori K; Kitazume S; Sato K; Maeda J; Higuchi M; Kizuka Y; Korekane H; Matsuo I; Honke K; Taniguchi N. 2013. Loss of branched O-mannosyl glycans in astrocytes accelerates remyelination. J Neurosci 33(24):10037-47. [PubMed: 23761899]  [MGI Ref ID J:199165]

Lee JK; Matthews RT; Lim JM; Swanier K; Wells L; Pierce JM. 2012. Developmental expression of the neuron-specific N-acetylglucosaminyltransferase Vb (GnT-Vb/IX) and identification of its in vivo glycan products in comparison with those of its paralog, GnT-V. J Biol Chem 287(34):28526-36. [PubMed: 22715095]  [MGI Ref ID J:188930]

Lee SU; Grigorian A; Pawling J; Chen IJ; Gao G; Mozaffar T; McKerlie C; Demetriou M. 2007. N-glycan processing deficiency promotes spontaneous inflammatory demyelination and neurodegeneration. J Biol Chem 282(46):33725-34. [PubMed: 17855338]  [MGI Ref ID J:128384]

Markowska AI; Liu FT; Panjwani N. 2010. Galectin-3 is an important mediator of VEGF- and bFGF-mediated angiogenic response. J Exp Med 207(9):1981-93. [PubMed: 20713592]  [MGI Ref ID J:165731]

Morgan R; Gao G; Pawling J; Dennis JW; Demetriou M; Li B. 2004. N-acetylglucosaminyltransferase v (Mgat5)-mediated N-glycosylation negatively regulates Th1 cytokine production by T cells. J Immunol 173(12):7200-8. [PubMed: 15585841]  [MGI Ref ID J:94864]

Ohtsubo K; Takamatsu S; Minowa MT; Yoshida A; Takeuchi M; Marth JD. 2005. Dietary and genetic control of glucose transporter 2 glycosylation promotes insulin secretion in suppressing diabetes. Cell 123(7):1307-21. [PubMed: 16377570]  [MGI Ref ID J:117542]

Partridge EA; Le Roy C; Di Guglielmo GM; Pawling J; Cheung P; Granovsky M; Nabi IR; Wrana JL; Dennis JW. 2004. Regulation of cytokine receptors by Golgi N-glycan processing and endocytosis. Science 306(5693):120-4. [PubMed: 15459394]  [MGI Ref ID J:93100]

Soleimani L; Roder JC; Dennis JW; Lipina T. 2008. Beta N-acetylglucosaminyltransferase V (Mgat5) deficiency reduces the depression-like phenotype in mice. Genes Brain Behav 7(3):334-43. [PubMed: 17883406]  [MGI Ref ID J:147477]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygous females and homozygous males. Homozygous females are infertile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)