Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N2F4pN1   Donating Investigator James Dennis,   Samuel Lunenfeld Research Institute

Description
No gene product (protein) is detected by Western blot analysis and enzyme activity is undetectable. Beta-galactosidase activity mimics endogenous gene expression patterns. Homozygotes exhibit abnormal increased leukocyte infiltration in kidney tissue, which is indicative of kidney autoimmune glomerulonephritis. Induced experimental autoimmune encephalomyelitis (EAE) and induced delayed-type hypersensitivity (DTH) responses are increased in homozygotes. Cultured splenocytes isolated from homozygotes produce 2 fold more IFN-gamma and 2 fold less IL4. Mutant mice exhibit delayed tumor progression when bred with oncomice (for example, when crossed to a polyomavirus middle T antigen expressing transgenic strain). Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygous females are not fertile. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, autoimmunity and antitumor immunity.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
A targeting vector containing neomycin resistance and beta-galactosidase genes was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting male chimeric animals were crossed to 129 female mice, and then backcrossed to C57BL/6 for 9 generations before arriving at The Jackson Laboratory.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Mgat5^tm1Jwd/Mgat5^tm1Jwd

        involves: 129S1/Sv * 129X1/SvJ

• immune system phenotype
• abnormal inflammatory response (MGI Ref ID J:60960)
  • abnormal recruitment of leukocytes into inflamed tissue
• abnormal leukocyte physiology (MGI Ref ID J:94864)
  • increased splenocyte proliferation after stimulation
• abnormal T cell physiology (MGI Ref ID J:60960)
  • T cells hypersensitive to T cell receptor agonists
• decreased interleukin-2 secretion (MGI Ref ID J:94864)
  • Il2 production is reduced
• decreased interleukin-4 secretion (MGI Ref ID J:94864)
  • Il4 production is reduced about 2 fold per cell
• increased interleukin-10 secretion (MGI Ref ID J:94864)
  • production is increased
• increased tumor necrosis factor secretion (MGI Ref ID J:94864)
  • TNF-alpha production is increased more than 2-fold
• tumorigenesis
• decreased metastatic potential (MGI Ref ID J:60960)
  • lung cancer metastasis about 50% of controls and never causes cardiac hypertrophy
• decreased tumor growth/size (MGI Ref ID J:60960)
  • tumor growth much slower (3.4g at 28-30 weeks as opposed to 15g in controls)
• decreased tumor incidence (MGI Ref ID J:60960)
  • 50% reduction in the incidence of mammary tumors at 24 weeks of age rather than at 16 weeks as in controls
  • male tumor development at 10-13 months of age rather than at 6-9 months
  • tumor foci development is normal
• renal/urinary system phenotype
• abnormal renal glomerulus morphology (MGI Ref ID J:60960)
  • age related reduction in cellularity of the kidney glomerulus
• behavior/neurological phenotype
• abnormal maternal nurturing (MGI Ref ID J:60960)
  • deficiencies in nurturing behavior

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Immunology and Inflammation Research
Autoimmunity

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mgat5tm1Jwd
Allele Name		targeted mutation 1, James W Dennis
Allele Type		Targeted (Reporter)
Common Name(s)		Mgat5-;
Mutation Made By		Peter Sobieszczuk,   Consortium for Functional Glycomics,TSRI
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Mgat5, mannoside acetylglucosaminyltransferase 5
Chromosome		1
Gene Common Name(s)		4930471A21Rik; 5330407H02Rik; AI480971; GNT-V; GNT-VA; GlcNAc-TV; RIKEN cDNA 4930471A21 gene; RIKEN cDNA 5330407H02 gene; beta1,6N-acetylglucosaminyltransferase V; expressed sequence AI480971;
Molecular Note		A LacZ and neomycin resistance cassette replaced part of exon 1. [MGI Ref ID J:60960]

Genotyping

Genotyping Information

Genotyping Protocols

Mgat5^tm1Jwd, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Granovsky M; Fata J; Pawling J; Muller WJ; Khokha R; Dennis JW. 2000. Suppression of tumor growth and metastasis in Mgat5-deficient mice. Nat Med 6(3):306-12. [PubMed: 10700233]  [MGI Ref ID J:60960]

Additional References

Mgat5^tm1Jwd related

Demetriou M; Granovsky M; Quaggin S; Dennis JW. 2001. Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation. Nature 409(6821):733-9. [PubMed: 11217864]  [MGI Ref ID J:111264]

Guo HB; Lee I; Bryan BT; Pierce M. 2005. Deletion of mouse embryo fibroblast N-acetylglucosaminyltransferase V stimulates alpha5beta1 integrin expression mediated by the protein kinase C signaling pathway. J Biol Chem 280(9):8332-42. [PubMed: 15615721]  [MGI Ref ID J:97247]

Guo HB; Lee I; Kamar M; Pierce M. 2003. N-acetylglucosaminyltransferase V expression levels regulate cadherin-associated homotypic cell-cell adhesion and intracellular signaling pathways. J Biol Chem 278(52):52412-24. [PubMed: 14561752]  [MGI Ref ID J:87101]

Guo HB; Nairn A; Harris K; Randolph M; Alvarez-Manilla G; Moremen K; Pierce M. 2008. Loss of expression of N-acetylglucosaminyltransferase Va results in altered gene expression of glycosyltransferases and galectins. FEBS Lett 582(4):527-35. [PubMed: 18230362]  [MGI Ref ID J:131841]

Lee SU; Grigorian A; Pawling J; Chen IJ; Gao G; Mozaffar T; McKerlie C; Demetriou M. 2007. N-glycan processing deficiency promotes spontaneous inflammatory demyelination and neurodegeneration. J Biol Chem 282(46):33725-34. [PubMed: 17855338]  [MGI Ref ID J:128384]

Morgan R; Gao G; Pawling J; Dennis JW; Demetriou M; Li B. 2004. N-acetylglucosaminyltransferase v (Mgat5)-mediated N-glycosylation negatively regulates Th1 cytokine production by T cells. J Immunol 173(12):7200-8. [PubMed: 15585841]  [MGI Ref ID J:94864]

Ohtsubo K; Takamatsu S; Minowa MT; Yoshida A; Takeuchi M; Marth JD. 2005. Dietary and genetic control of glucose transporter 2 glycosylation promotes insulin secretion in suppressing diabetes. Cell 123(7):1307-21. [PubMed: 16377570]  [MGI Ref ID J:117542]

Partridge EA; Le Roy C; Di Guglielmo GM; Pawling J; Cheung P; Granovsky M; Nabi IR; Wrana JL; Dennis JW. 2004. Regulation of cytokine receptors by Golgi N-glycan processing and endocytosis. Science 306(5693):120-4. [PubMed: 15459394]  [MGI Ref ID J:93100]

Soleimani L; Roder JC; Dennis JW; Lipina T. 2008. Beta N-acetylglucosaminyltransferase V (Mgat5) deficiency reduces the depression-like phenotype in mice. Genes Brain Behav 7(3):334-43. [PubMed: 17883406]  [MGI Ref ID J:147477]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygous females and homozygous males. Homozygous females are infertile.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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