Strain Name: |
B6.129-Selplgtm1Rpmc/J |
|---|---|
Stock Number: |
006336 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Selplg; Selplgtm1Rpmc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Stock Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Richard Cummings, University of Oklahoma Generation N4+ (07-AUG-07) Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by flow cytometry analysis of leukocytes. Homozygotes have elevated total leukocyte counts, with increased numbers of neutrophils, lympohcytes and eosinophils. Leukocytes isolated from homozygotes exhibit abnormal tethering and rolling (adhesion and migration) due to impaired attachment. This mutant mouse strain represents a model that may be useful in studies of leukocyte adhesion and migration in the inflammatory response.
This strain was transferred from the collection of the Consortium for Functional Glycomics.Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the 5’ end of exon 2 and the ATG translational start site sequence. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J/+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. Heterozygotes were crossed to generate homozygotes. The mice were then backcrossed to C57BL/6J for 5 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Selplgtm1Rpmc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Rodger P McEver | ||
| Common Name(s) | PSGL-1-; | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | CJ7 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Gene Symbol and Name | Selplg, selectin, platelet (p-selectin) ligand | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CD162; CLA; PSGL-1; PSGL1; Selpl; | ||
| Molecular Note | A neomycin resistance cassette replaced the 5' portion of exon 2, which includes the translation initiation start site, via homologous recombination. Flow cytometry was used to confirm the absence of gene expression in leukocytes of homozygous mutant animals. [MGI Ref ID J:75829] [MGI Ref ID J:95268] | ||
Genotyping Protocols
Selplgtm1Rpmc
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Selplg
004201 B6.Cg-Selplgtm1Fur/J View Strains carrying other alleles of Selplg (1 strain)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cardiovascular Research
Vascular Defects (defective leukocyte function)
Immunology and Inflammation Research
Inflammation (Neutrophil defects)
References
Selected Reference(s)
Additional ReferencesXia L; Sperandio M; Yago T; McDaniel JM; Cummings RD; Pearson-White S; Ley K; McEver RP. 2002. P-selectin glycoprotein ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow. J Clin Invest 109(7):939-50. [PubMed: 11927621] [MGI Ref ID J:75829]
Price and Supply Information
| Strain Name: | B6.129-Selplgtm1Rpmc/J |
| Stock Number: | 006336 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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