Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N5F2pN1   Donating Investigator Richard Cummings,   University of Oklahoma

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by flow cytometry analysis of leukocytes. Homozygotes have elevated total leukocyte counts, with increased numbers of neutrophils, lympohcytes and eosinophils. Leukocytes isolated from homozygotes exhibit abnormal tethering and rolling (adhesion and migration) due to impaired attachment. This mutant mouse strain represents a model that may be useful in studies of leukocyte adhesion and migration in the inflammatory response.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the 5’ end of exon 2 and the ATG translational start site sequence. The construct was electroporated into 129S1/Sv-p⁺ Tyr⁺ Kitl^Sl-J/+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. Heterozygotes were crossed to generate homozygotes. The mice were then backcrossed to C57BL/6J for 5 generations.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Selplg

004201

B6.Cg-Selplgtm1Fur/J

View Strains carrying other alleles of Selplg     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Selplg^tm1Rpmc/Selplg^tm1Rpmc

        involves: 129S1/Sv * C57BL/6J

• immune system phenotype
• abnormal leukocyte adhesion (MGI Ref ID J:75829)
  • tethering to E-selectin is impaired
• abnormal leukocyte rolling (MGI Ref ID J:138731)
  • fewer leukocytes roll on E-selectin coated substrates and no leukocytes roll on P-selectin coated substrates unlike wild-type cells
  • rolling on TNFa-treated venules is reduced compared to in wild-type venules
• increased leukocyte cell number (MGI Ref ID J:75829)
• increased eosinophil cell number (MGI Ref ID J:75829)
• increased lymphocyte cell number (MGI Ref ID J:75829)
• increased neutrophil cell number (MGI Ref ID J:75829)
• homeostasis/metabolism phenotype
• increased bleeding time (MGI Ref ID J:138731)
  • slower clotting times
• hematopoietic system phenotype
• increased leukocyte cell number (MGI Ref ID J:75829)
• increased eosinophil cell number (MGI Ref ID J:75829)
• increased lymphocyte cell number (MGI Ref ID J:75829)
• increased neutrophil cell number (MGI Ref ID J:75829)

Selplg^tm1Rpmc/Selplg^tm1Rpmc

        involves: 129S1/Sv

• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:115682)
  • mice exhibit a normal infection susceptibility to A. phagocytophilum
• abnormal chemokine secretion (MGI Ref ID J:115682)
  • chemokine secretion in response to infection with A. phagocytophilum is greater than in wild-type mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects
      defective leukocyte function

Immunology and Inflammation Research
Inflammation
      Neutrophil defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Selplgtm1Rpmc
Allele Name		targeted mutation 1, Rodger P McEver
Allele Type		Targeted (knock-out)
Common Name(s)		PSGL-1-;
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		CJ7
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Selplg, selectin, platelet (p-selectin) ligand
Chromosome		5
Gene Common Name(s)		CD162; CLA; PSGL-1; PSGL1; Selpl;
Molecular Note		A neomycin resistance cassette replaced the 5' portion of exon 2, which includes the translation initiation start site, via homologous recombination. Flow cytometry was used to confirm the absence of gene expression in leukocytes of homozygous mutant animals. [MGI Ref ID J:75829] [MGI Ref ID J:95268]

Genotyping

Genotyping Information

Genotyping Protocols

Selplg^tm1Rpmc, Separated PCR
Selplg^tm1Rpmc, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Xia L; Sperandio M; Yago T; McDaniel JM; Cummings RD; Pearson-White S; Ley K; McEver RP. 2002. P-selectin glycoprotein ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow. J Clin Invest 109(7):939-50. [PubMed: 11927621]  [MGI Ref ID J:75829]

Additional References

Selplg^tm1Rpmc related

Carlyon JA; Akkoyunlu M; Xia L; Yago T; Wang T; Cummings RD; McEver RP; Fikrig E. 2003. Murine neutrophils require alpha1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. Blood 102(9):3387-95. [PubMed: 12869507]  [MGI Ref ID J:115682]

Fabene PF; Mora GN; Martinello M; Rossi B; Merigo F; Ottoboni L; Bach S; Angiari S; Benati D; Chakir A; Zanetti L; Schio F; Osculati A; Marzola P; Nicolato E; Homeister JW; Xia L; Lowe JB; McEver RP; Osculati F; Sbarbati A; Butcher EC; Constantin G. 2008. A role for leukocyte-endothelial adhesion mechanisms in epilepsy. Nat Med 14(12):1377-83. [PubMed: 19029985]  [MGI Ref ID J:142251]

Gauguet JM; Rosen SD; Marth JD; von Andrian UH. 2004. Core 2 branching beta1,6-N-acetylglucosaminyltransferase and high endothelial cell N-acetylglucosamine-6-sulfotransferase exert differential control over B- and T-lymphocyte homing to peripheral lymph nodes. Blood 104(13):4104-12. [PubMed: 15319280]  [MGI Ref ID J:95268]

Hrachovinova I; Cambien B; Hafezi-Moghadam A; Kappelmayer J; Camphausen RT; Widom A; Xia L; Kazazian HH Jr; Schaub RG; McEver RP; Wagner DD. 2003. Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A. Nat Med 9(8):1020-5. [PubMed: 12858167]  [MGI Ref ID J:99599]

Mazo IB; Honczarenko M; Leung H; Cavanagh LL; Bonasio R; Weninger W; Engelke K; Xia L; McEver RP; Koni PA; Silberstein LE; von Andrian UH. 2005. Bone marrow is a major reservoir and site of recruitment for central memory CD8+ T cells. Immunity 22(2):259-70. [PubMed: 15723813]  [MGI Ref ID J:96675]

Miner JJ; Xia L; Yago T; Kappelmayer J; Liu Z; Klopocki AG; Shao B; McDaniel JM; Setiadi H; Schmidtke DW; McEver RP. 2008. Separable requirements for cytoplasmic domain of PSGL-1 in leukocyte rolling and signaling under flow. Blood 112(5):2035-45. [PubMed: 18550846]  [MGI Ref ID J:138731]

Smith ML; Olson TS; Ley K. 2004. CXCR2- and E-selectin-induced neutrophil arrest during inflammation in vivo. J Exp Med 200(7):935-9. [PubMed: 15466624]  [MGI Ref ID J:93946]

Zarbock A; Lowell CA; Ley K. 2007. Spleen tyrosine kinase Syk is necessary for E-selectin-induced alpha(L)beta(2) integrin-mediated rolling on intercellular adhesion molecule-1. Immunity 26(6):773-83. [PubMed: 17543554]  [MGI Ref ID J:123587]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.