Go to JAX® Mice Query Form

Strain Name:

B6.Cg-Lgals1tm1Rob/J

Stock Number:

006337

Availability:

Repository- Live

View Pricing for USA, Canada and Mexico
View Pricing for International

General Terms and Conditions
Additional Licensing and Use Restrictions

Genes & Alleles   Lgals1;   Lgals1tm1Rob;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Richard Cummings,   University of Oklahoma
GenerationN5+N1F1 (25-APR-08)

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (mRNA or protein) is detected by in situ hybridization of dorsal root ganglia and facial motorneuron nucleus and Western blot analysis of adult muscle tissue. Neonate mice homozygous for the mutation have abnormal axon targeting to the caudal region of the olfactory bulb. Mutant mice have fewer neural progenitor cells in the subventricular zone of the forebrain, although the number of apoptotic cells are not affected. Homozygotes exhibit hypoalgesia with a diminished nocifensive withdraw response to thermal testing. Immunohistological analysis of dorsal root ganglia from homozygotes reveals abnormal proportions of axon subpopulations and a larger number of myelinated axons. Mutant mice have a longer recovery of motorneuron function after experimental nerve injury. This mutant mouse strain represents a model that may be useful in studies of pain reception, motorneuron regeneration, axonal growth and guidance and development of the nervous system.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 1.0kb of sequence that includes exon 2. The construct was electroporated into 129S6/SvEvTac derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into outbred MF-1 blastocysts. The resulting chimeric male animals were crossed to outbred MF-1 female mice. Heterozygotes were crossed to generate homozygotes. The mice were then bred to mice homozygous for a targeted mutation of Lgals3. The double mutant strain was then backcrossed to C57BL/6 for 5 generations. Upon arrival at The Jackson Laboratory, the second allele was removed by selective breeding. These mice carry only the Lgals1tm1Rob allele.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Lgals1tm1Rob/Lgals1tm1Rob

        involves: 129S/SvEv * MF1
  • nervous system phenotype
  • abnormal olfactory nerve morphology (MGI Ref ID J:36694)
    • although the olfactory nerve itself was normal, projections from it to the dorsocaudal surface of the olfactory bulb were severly reduced and axons never terminated in glomeruli
    • mice were otherwise normal
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:16245)
    • mice are normal

Lgals1tm1Rob/Lgals1tm1Rob

        involves: 129S/SvEv
  • nervous system phenotype
  • abnormal dorsal root ganglion morphology (MGI Ref ID J:101850)
    • mice exhibit fewer IB4-binding neurons (non-peptidergic nociceptor neurons), more NF200-exressing neurons (large-diameter mechanoreceptor neurons) and more myelinated sensory axons in the dorsal root ganglia than in wild type mice
    • the mean diameter of neurons in the dorsal root ganglia is reduced compared to in wild type mice
    • however, the total number of neurons in the dorsal root ganglia is normal
  • abnormal neuromuscular synapse morphology (MGI Ref ID J:122104)
    • mice exhibit a delay in eliminating nerve endings after axotomy compared to in wild type mice
  • abnormal postnatal subventricular zone morphology (MGI Ref ID J:109590)
    • mice exhibit fewer proliferating cells and slowly dividing cells in the adult subventricular zone (SVZ) compared to in wild type mice
    • fewer progenitor cells are found in the adult SVZ compared to in wild type mice
    • however, the number of apoptotic cells in the SVZ is normal
  • abnormal sensory neuron morphology (MGI Ref ID J:101850)
    • mice exhibit more myelinated sensory axons in the dorsal root ganglia than in wild type mice
    • abnormal mechanoreceptor morphology (MGI Ref ID J:101850)
      • mice exhibit fewer IB4-binding neurons (non-peptidergic nociceptor neurons) in the dorsal root ganglia than in wild type mice
      • IB4-binding axons extend deeper into the dorsal horn of the spinal cord than in wild type mice
    • abnormal nociceptor morphology (MGI Ref ID J:101850)
      • mice exhibit more myelinated sensory axons in the dorsal root ganglia than in wild type mice
    • abnormal olfactory neuron morphology (MGI Ref ID J:36694)
      • olfactory axons exhibit abnormal projections in the dorsocaudal olfactory bulb and, unlike in wild type mice most axons do not terminate in discrete glomeruli
  • immune system phenotype
  • increased interferon-gamma secretion (MGI Ref ID J:123416)
    • stimulated CD4+ T cells secrete more IFN-gamma than wild type mice
  • increased interleukin-2 secretion (MGI Ref ID J:123416)
    • stimulated CD4+ T cells secrete more IL-2 than wild type mice
  • increased susceptibility to experimental autoimmune encephalomyelitis (MGI Ref ID J:123416)
    • when treated to induce experimental autoimmune encephalomyelitis, mice exhibit a more severe disease, vigorous splenocyte proliferation, IL-17 and IFN-gamma production and a greater accumulation of MOG35-55-specific TH1 and TH-17 cells compared to in similarly treated wild type mice
  • muscle phenotype
  • delayed muscle development (MGI Ref ID J:119480)
    • mice exhibit a delay in muscle development beginning at day 1 through day 7
  • touch/vibrissae phenotype
  • increased thermal nociceptive threshold (MGI Ref ID J:101850)
  • behavior/neurological phenotype
  • increased thermal nociceptive threshold (MGI Ref ID J:101850)

Lgals1tm1Rob/Lgals1tm1Rob

        involves: 129S/SvEv * 129S1/SvImJ * DBA/2J
  • immune system phenotype
  • abnormal cytokine secretion (MGI Ref ID J:130208)
    • following treatment with dydrogesterone, uterine support cells exhibit an increase in TH1 to TH2 cytokine ratio compared to wild type mice
  • reproductive system phenotype
  • *normal* reproductive system phenotype (MGI Ref ID J:130208)
    • mice exhibit more fetal loss than in wild type mice without altered number of implantation sites or litter size

Gene & Allele Details

Allele Symbol Lgals1tm1Rob
Allele Name targeted mutation 1, Elizabeth J Robertson
Common Name(s) gal-1-; gal1-;
Mutation Made By Richard Cummings,   University of Oklahoma
Strain of Origin129S/SvEv-Gpi1
ES Cell Line NameCCE/EK.CCE
ES Cell Line Strain129S/SvEv-Gpi1
Gene Symbol and Name Lgals1, lectin, galactose binding, soluble 1
Chromosome 15
Gene Common Name(s) AA410090; DKFZp686E23103; GAL1; GBP; Gal-1; Galbp; L14; Lect14; beta galactoside binding protein; expressed sequence AA410090; galectin-1; lactose binding soluble lectin, 14 kDa;
Molecular Note Exon 2, encoding the carbohydrate-binding domain, was deleted by the insertion of neomycin selection cassette. Western blot analysis and immunostaining of sections showed an absence of encoded protein in muscle tissue obtained from adult homozygous mutant mice. [MGI Ref ID J:16245]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Lgals1tm1Rob

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Lgals1tm1Rob allele
006354   B6.Cg-Lgals3tm1Poi Lgals1tm1Rob/J
View Strains carrying   Lgals1tm1Rob     (1 strain)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Neurodevelopmental Defects

Sensorineural Research
Nociception

References

Selected Reference(s)

Poirier F; Robertson EJ. 1993. Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin. Development 119(4):1229-36. [PubMed: 8306885]  [MGI Ref ID J:16245]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Lgals1tm1Rob/J
Stock Number: 006337

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

*NO Shipping Destination selected!

 

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX® Mice Query Form

(2.15)