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Strain Name:

B6.Cg-Lgals3tm1Poi/J

Stock Number:

006338

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Genes & Alleles   Lgals3;   Lgals3tm1Poi;

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Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Richard Cummings,   University of Oklahoma
GenerationN5+N1F2 (02-MAY-08)

Strain Description
Homozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavoiral abnormalities. No gene product (mRNA or protein) is detected by in situ hybridization of tibia bones sections from embryonic day 16.4 mice or by immunohistological staining of fetal skin. Homozygotes have an impaired acute inflammation response. Initial inflammatory infiltrate cell recruitment is normal, but four days after intraperitoneal injection of thioglycollate, mutant mice have a four-fold lower number of recruited granulocytes. Mutant mice have impaired chondrocyte differentiation during long bone development. Fewer hypertrophic chondrocytes but more empty lacunae and condensed chondrocytes are found in the chondrovascular junction. Chondrocytes, cartilage matrix and lacunae are morphologically abnormal. Carbon tetrachloride-induced liver fibrosis results in reduced collagen deposition when compared to wildtype controls. Mutant mice also display defective myofibroblast activation. This mutant mouse strain may be useful in studies of bone development, endochondral ossification, inflammatory response, and liver fibrosis.

This strain was transferred from the Consortium for Functional Glycomics strain collection.

Strain Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 3.7kb of sequence that includes exons 2, 3 and 4. The construct was electroporated into WW6 embryonic stem (ES) cells (derived from 129/Sv, C57BL/6, SJL mixed background mice). Correctly targeted ES cells were injected into outbred MF-1 blastocysts. The resulting chimeric male animals were crossed to 129 female mice. Heterozygotes were crossed to generate homozygotes. The mice were then bred to mice homozygous for a targeted mutation of Lgals1, and he double mutant strain was backcrossed to C57BL/6 for 5 generations. Upon arrival at The Jackson Laboratory, the Lgals1mutation was removed by selective breeding; therefore this strain carries only the Lgals3tm1Poi allele.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Lgals3tm1Poi/Lgals3tm1Poi

        involves: 129 * 129/Sv * C57BL/6J * SJL
  • life span-post-weaning/aging
  • *normal* life span-post-weaning/aging (MGI Ref ID J:47227)
    • mice exhibit a normal lifespan
  • reproductive system phenotype
  • *normal* reproductive system phenotype (MGI Ref ID J:47227)
    • mice exhibit normal reproduction
  • skin/coat/nails phenotype
  • *normal* skin/coat/nails phenotype (MGI Ref ID J:47227)
    • mice exhibit normal skin structure

Lgals3tm1Poi/Lgals3tm1Poi

        involves: 129/Sv * C57BL/6 * SJL
  • life span-post-weaning/aging
  • abnormal induced morbidity/mortality (MGI Ref ID J:131407)
    • mice infected with S. pneumoniae exhibit high mortality after 24 hours
  • immune system phenotype
  • abnormal macrophage physiology (MGI Ref ID J:131971)
    • macrophage activation by IL-4 and IL-13 stimulation Is impaired
    • however, macrophage activation by IFN-gamma and LPS is normal
    • impaired macrophage phagocytosis (MGI Ref ID J:131407)
      • phagocytosis of apoptotic neutrophils by bone marrow-derived macrophages is impaired
      • however, phagocytosis of S. pneumonia is normal
    • impaired macrophage recruitment (MGI Ref ID J:131971)
      • when stimulated by IL-4 and IL-13
  • impaired neutrophil recruitment (MGI Ref ID J:131407)
    • 15 hours after infection with S. pneumoniae
    • however, myeloperoxidase activity is similar to in wild type mice 15 hours after infection with S. pneumoniae
  • increased interleukin-6 secretion (MGI Ref ID J:131407)
    • 15 hours after infection with S. pneumoniae, IL-6 concentration in bronchoalveolar lavage is increased compared to in wild type mice
  • increased susceptibility to bacterial infection (MGI Ref ID J:131407)
    • mice infected with S. pneumoniae exhibit high mortality after 24 hours
    • after 15 hours, mice infected with S. pneumoniae exhibit increased cell infiltrate but decreased total cell counts in the lungs and develop severe pneumonia with increased lung injury and septicemia compared to wild type mice
    • after 15 hours, mice infected with S. pneumoniae exhibit a 450-fold increase in bacterial load compared to wild type mice and blood cultures indicate 100 % bacteremia compared to 30% in wild type mice
  • increased tumor necrosis factor secretion (MGI Ref ID J:131407)
    • 15 hours after infection with S. pneumoniae, TNF-alpha concentration in bronchoalveolar lavage is increased compared to in wild type mice
  • lung inflammation (MGI Ref ID J:131407)
    • after 15 hours, mice infected with S. pneumoniae exhibit increased cell infiltrate in the lungs and develop severe pneumonia with increased lung injury and septicemia compared to wild type mice
  • sepsis (MGI Ref ID J:131407)
    • after 15 hours, mice infected with S. pneumoniae exhibit septicemia unlike wild type mice
  • skeleton phenotype
  • abnormal long bone epiphyseal plate morphology (MGI Ref ID J:66902)
    • unlike in wild type mice, a large zone of empty lacunae is observed between the last row of hypertrophic cells and the vascular invasion front
    • abnormal long bone hypertrophic chondrocyte zone (MGI Ref ID J:66902)
      • chondrocytes within the hypertrophic zone are larger and more vacuolated than in wild type mice
      • mice exhibit an increased number of nonhypertrophic cells in the late hyprtrophic zone compared to in wild type mice
      • chondrocytes in the zone contain unusually large intracellular glycogen aggregates
      • decreased width of hypertrophic chondrocyte zone (MGI Ref ID J:66902)
        • the hypertrophic zone is reduced in size and contains 20% fewer hypertrophic cells than in wild type mice
  • renal/urinary system phenotype
  • abnormal kidney physiology (MGI Ref ID J:131371)
    • mice exhibit less renal fibrosis following unilateral ureter obstruction (UUO) compared to wild type mice
    • however, macrophage recruitment and cytokine production induced by UUO is normal
  • respiratory system phenotype
  • lung inflammation (MGI Ref ID J:131407)
    • after 15 hours, mice infected with S. pneumoniae exhibit increased cell infiltrate in the lungs and develop severe pneumonia with increased lung injury and septicemia compared to wild type mice
  • homeostasis/metabolism phenotype
  • decreased susceptibility to injury (MGI Ref ID J:131371)
    • mice exhibit less renal fibrosis following unilateral ureter obstruction (UUO) compared to wild type mice
    • however, macrophage recruitment and cytokine production induced by UUO is normal

Lgals3tm1Poi/Lgals3tm1Poi

        involves: 129/Sv * C57BL/6 * MF1 * SJL
  • hematopoietic system phenotype
  • decreased granulocyte number (MGI Ref ID J:110422)
    • while recruitment of granulocytes following treatment with thioglycolate is normal, granulocyte numbers 3 to 4 days after treatment are reduced 4-fold compared to in wild type mice
    • however, granulocyte apoptosis rates and phagocytosis of apoptotic granulocytes are normal
  • immune system phenotype
  • decreased granulocyte number (MGI Ref ID J:110422)
    • while recruitment of granulocytes following treatment with thioglycolate is normal, granulocyte numbers 3 to 4 days after treatment are reduced 4-fold compared to in wild type mice
    • however, granulocyte apoptosis rates and phagocytosis of apoptotic granulocytes are normal

Gene & Allele Details

Allele Symbol Lgals3tm1Poi
Allele Name targeted mutation 1, Francoise Poirier
Common Name(s) GaL3-; galectin 3<->;
Mutation Made By Richard Cummings,   University of Oklahoma
Strain of OriginSTOCK 129/Sv and C57BL/6J and SJL
ES Cell Line NameWW6
ES Cell Line StrainSTOCK 129/Sv and C57BL/6J and SJL
Gene Symbol and Name Lgals3, lectin, galactose binding, soluble 3
Chromosome 14
Gene Common Name(s) CBP35; GAL3; GALBP; GALIG; L-34; LGALS2; MAC2; MGC105387; Mac-2; gal-3; galectin-3;
Molecular Note 3.7kb of sequence, encompassing exons 2 through 4, was replaced via the insertion of a neomycin selection cassette. Immunostaining of fetal skin showed an absence of protein in homozygous mutant embryos. [MGI Ref ID J:47227]

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Lgals3tm1Poi

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Lgals3tm1Poi allele
006354   B6.Cg-Lgals3tm1Poi Lgals1tm1Rob/J
View Strains carrying   Lgals3tm1Poi     (1 strain)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Skeletal Defects

Immunology and Inflammation Research
Inflammation

References

Selected Reference(s)

Colnot C; Fowlis D; Ripoche MA; Bouchaert I; Poirier F. 1998. Embryonic implantation in galectin 1/galectin 3 double mutant mice. Dev Dyn 211(4):306-13. [PubMed: 9566950]  [MGI Ref ID J:47227]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Lgals3tm1Poi/J
Stock Number: 006338

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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