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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
Strain Information
Former Names STOCK Tg(Gad1/EGFP)98Agmo/J (Changed: 14-DEC-07 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Generation ?+F1 (28-DEC-07) Donating Investigator Ariel Agmon, West Virginia Univ School of Medicine Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the mouse Gad1 (glutamic acid decarboxylase 1 or GAD67) promoter. In the neocortex, EGFP expression is detected predominantly in layers 5B and 6, and to a lesser extent in layers 2/3. Low levels of fluorescence are detected in small cells with glial morphology. EGFP expression is also observed in area CA1 of the hippocampus, mainly in large interneurons in the oriens-alveus layers. In the neocortex of founder line 98 transgenic mice, fluorescence is observed in infragranular, calbindin immunoreactive interneurons with axonal arborizations in layer 1. In animals younger than two weeks of age, an increased number of EGFP labeled neurons is observed in the supragranular layers, and EGFP expression is also observed in some cells with pyramidal morphology. The fluorescently labeled populations of neurons in this founder line is distinct from the subset of somatostatin-containing (SOM+) interneurons in founder line 94 mice (Stock No. 006334), or in mice expressing Tg(GadGFP)45704Swn (Stock No. 003718), in location, neurochemical markers, axonal morphologies, and electrophysiological characteristics. This mutant mouse strain may be useful in studies of populations of inhibitory interneurons of the hippocampus and neocortex.Development
A transgenic construct containing EGFP gene under the control of the mouse Gad1, glutamic acid decarboxylase 1, promoter was injected into fertilized C57BL6 X SJL mouse eggs. Founder line 98 was established. Animals were initially bred to C57BL/6 mice and then bred to outbred CD-1 mice. The mice were then bred to outbred Hilltop Swiss ICR mice. The Donating Investigator maintained the strain by mating hemizygotes to outbred mice.
Control Information
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (169 strains)
007677 CB6-Tg(Gad1-EGFP)G42Zjh/J 003718 FVB-Tg(GadGFP)45704Swn/J 006334 STOCK Tg(Gad1-EGFP)94Agmo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(Gad1-EGFP)98Agmo/0
involves: C57BL/6 * CD-1 * SJL
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:117601)
- mice are viable and fertile and show no obvious phenotypic abnormalities
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Tg(Gad1-EGFP)98Agmo relatedResearch Tools
Fluorescent Proteins
Neurobiology Research
Alzheimer's Disease
Parkinson's Disease
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Gad1-EGFP)98Agmo | ||
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| Allele Name | transgene insertion 98, Ariel Agmon | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | GAD67-GFP; Tg(Gad1/EGFP)98Agmo; X98; | ||
| Mutation Made By | Ariel Agmon, West Virginia Univ School of Medicine | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | neocortex (predominantly in layers 5B and 6, and to a lesser extent in layers 2/3; low levels of fluorescence are detected in small cells with glial morphology; infragranular, calbindin immunoreactive interneurons with axonal arborizations in layer 1); area CA1 of the hippocampus (mainly in large interneurons in the oriens-alveus layers); animals younger than two weeks of age show an increased number of EGFP labeled neurons is in the supragranular layers, and some cells with pyramidal morphology | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gad1, glutamic acid decarboxylase 1, mouse, laboratory | ||
| General Note | Of 11 initial lines derived from founders shown by PCR analysis to carry the transgene, 5 that expressed EGFP in the brain continued to be maintained. Two of these, designated by the authors X94 and X98, in which cortical EGFP expression was limited to nonpyramidal cells were subjected to further analysis. | ||
| Molecular Note | The transgene comprises 10.3 kb of mouse genomic DNA containing the promoter region from the glutamic acid decarboxylase 1 (Gad1, commonly called GAD67) gene, including the 5' regulatory sequences, (noncoding) exon 1, intron 1 and a segment of exon 2, joined in-frame to the coding sequence of enhanced green fluorescent protein followed by an SV40 polyadenylation signal. The encoded fusion protein contains the first 17 amino acids of GAD1, 4 amino acids derived from the pEGFP-1 vector, and the entire enhanced green fluorescent protein. GFP expression is found in infragranular, calbindin-containing, layer 1-targeting cells that have propensity to fire low-threshold calcium spikes. [MGI Ref ID J:117601] [MGI Ref ID J:117729] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Gad1-EGFP)94Agmo, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Ma Y; Hu H; Berrebi AS; Mathers PH; Agmon A. 2006. Distinct subtypes of somatostatin-containing neocortical interneurons revealed in transgenic mice. J Neurosci 26(19):5069-82. [PubMed: 16687498] [MGI Ref ID J:117601]
Additional References
Tg(Gad1-EGFP)98Agmo relatedJin X; Mathers PH; Szabo G; Katarova Z; Agmon A. 2001. Vertical bias in dendritic trees of non-pyramidal neocortical neurons expressing GAD67-GFP in vitro. Cereb Cortex 11(7):666-78. [PubMed: 11415968] [MGI Ref ID J:117729]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System +/+ sibling x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Hemizygous for Tg(Gad1-EGFP)98Agmo *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $288.65 Hemizygous for Tg(Gad1-EGFP)98Agmo x Noncarrier $288.65 Noncarrier x Hemizygous for Tg(Gad1-EGFP)98Agmo
Additional Supply Details
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Hemizygous for Tg(Gad1-EGFP)98Agmo *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $375.30 Hemizygous for Tg(Gad1-EGFP)98Agmo x Noncarrier $375.30 Noncarrier x Hemizygous for Tg(Gad1-EGFP)98Agmo
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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