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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(Gad1/EGFP)98Agmo/J (Changed: 14-DEC-07 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Ariel Agmon, West Virginia Univ School of Medicine Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the mouse Gad1 (glutamic acid decarboxylase 1 or GAD67) promoter. In the neocortex, EGFP expression is detected predominantly in layers 5B and 6, and to a lesser extent in layers 2/3. Low levels of fluorescence are detected in small cells with glial morphology. EGFP expression is also observed in area CA1 of the hippocampus, mainly in large interneurons in the oriens-alveus layers. In the neocortex of founder line 98 transgenic mice, fluorescence is observed in infragranular, calbindin immunoreactive interneurons with axonal arborizations in layer 1. In animals younger than two weeks of age, an increased number of EGFP labeled neurons is observed in the supragranular layers, and EGFP expression is also observed in some cells with pyramidal morphology. The fluorescently labeled populations of neurons in this founder line is distinct from the subset of somatostatin-containing (SOM+) interneurons in founder line 94 mice (Stock No. 006334), or in mice expressing Tg(GadGFP)45704Swn (Stock No. 003718), in location, neurochemical markers, axonal morphologies, and electrophysiological characteristics. This mutant mouse strain may be useful in studies of populations of inhibitory interneurons of the hippocampus and neocortex.Development
A transgenic construct containing EGFP gene under the control of the mouse Gad1, glutamic acid decarboxylase 1, promoter was injected into fertilized C57BL6 X SJL mouse eggs. Founder line 98 was established. Animals were initially bred to C57BL/6 mice and then bred to outbred CD-1 mice. The mice were then bred to outbred Hilltop Swiss ICR mice. The Donating Investigator maintained the strain by mating hemizygotes to outbred mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
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012844 B6.Cg-Gad1tm1.1Bgc/J 007673 B6.Cg-Tg(Gad1-EGFP)3Gfng/J 007677 CB6-Tg(Gad1-EGFP)G42Zjh/J 003718 FVB-Tg(GadGFP)45704Swn/J 006334 STOCK Tg(Gad1-EGFP)94Agmo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
assigned by genotype
Tg(Gad1-EGFP)98Agmo/0
involves: C57BL/6 * CD-1 * SJL
- normal phenotype
- no abnormal phenotype detected
- mice are viable and fertile and show no obvious phenotypic abnormalities (MGI Ref ID J:117601)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Tg(Gad1-EGFP)98Agmo relatedResearch Tools
Fluorescent Proteins
Neurobiology Research
Alzheimer's Disease
Parkinson's Disease
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Gad1-EGFP)98Agmo | ||
|---|---|---|---|
| Allele Name | transgene insertion 98, Ariel Agmon | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | GAD67-GFP; Tg(Gad1/EGFP)98Agmo; X98; | ||
| Mutation Made By | Ariel Agmon, West Virginia Univ School of Medicine | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Site of Expression | neocortex (predominantly in layers 5B and 6, and to a lesser extent in layers 2/3; low levels of fluorescence are detected in small cells with glial morphology; infragranular, calbindin immunoreactive interneurons with axonal arborizations in layer 1); area CA1 of the hippocampus (mainly in large interneurons in the oriens-alveus layers); animals younger than two weeks of age show an increased number of EGFP labeled neurons is in the supragranular layers, and some cells with pyramidal morphology | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gad1, glutamate decarboxylase 1, mouse, laboratory | ||
| General Note | Of 11 initial lines derived from founders shown by PCR analysis to carry the transgene, 5 that expressed EGFP in the brain continued to be maintained. Two of these, designated by the authors X94 and X98, in which cortical EGFP expression was limited to nonpyramidal cells were subjected to further analysis. | ||
| Molecular Note | The transgene comprises 10.3 kb of mouse genomic DNA containing the promoter region from the glutamic acid decarboxylase 1 (Gad1, commonly called GAD67) gene, including the 5' regulatory sequences, (noncoding) exon 1, intron 1 and a segment of exon 2, joined in-frame to the coding sequence of enhanced green fluorescent protein followed by an SV40 polyadenylation signal. The encoded fusion protein contains the first 17 amino acids of GAD1, 4 amino acids derived from the pEGFP-1 vector, and the entire enhanced green fluorescent protein. GFP expression is found in infragranular, calbindin-containing, layer 1-targeting cells that have propensity to fire low-threshold calcium spikes. [MGI Ref ID J:117601] [MGI Ref ID J:117729] | ||
| Gene Symbol and Name | Tg(Gad1-EGFP)98Agmo, transgene insertion 98, Ariel Agmon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | GAD67-GFP; Tg(Gad1/EGFP)98Agmo; X98; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Gad1-EGFP)98Agmo MCA, Melt Curve Analysis
Tg(Gad1-EGFP)94Agmo, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ma Y; Hu H; Berrebi AS; Mathers PH; Agmon A. 2006. Distinct subtypes of somatostatin-containing neocortical interneurons revealed in transgenic mice. J Neurosci 26(19):5069-82. [PubMed: 16687498] [MGI Ref ID J:117601]
Additional References
Tg(Gad1-EGFP)98Agmo relatedJin X; Mathers PH; Szabo G; Katarova Z; Agmon A. 2001. Vertical bias in dendritic trees of non-pyramidal neocortical neurons expressing GAD67-GFP in vitro. Cereb Cortex 11(7):666-78. [PubMed: 11415968] [MGI Ref ID J:117729]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as hemizygotes. The Donating Investigator maintained this strain by mating hemizygotes to outbred mice.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
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