Strain Name: |
NOD.129S4(B6)-Icam1tm1Jcgr/J |
|---|---|
Stock Number: |
006351 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Icam1; Icam1tm1Jcgr; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Background Strain NOD/ShiLtJ Donor Strain 129S4 (J1 ES cell) Donating Investigator Stephan Martin, Dusseldorf at the Heinrich-Heine-Univers H2 Haplotype g7 Generation N2F3p Strain Description
Homozygous mutant mice are diabetes resistant. No membrane bound ICAM-1 is detectable in homozygous mutant mice. Histological examination shows no or only mild peri-insular infiltration at 330-410 days of age, By comparison, 64% of their wild-type and 44% of their heterozygote cohorts become diabetic by 310 days of age. In contrast to wild-type, 110-day-old ICAM-1 deficient females do not develop severe insulitis 10 days post cyclophosphamide treatment. Protection is associated with suppression of destructive insulitis as well as a deviation to a T-helper 2 cytokine mRNA expression pattern (Martin et al 2001).This targeted mutant strain is useful for studying the ICAM-1/LAF-1 interaction and its role in diabetes.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
Intercellular adhesion molecule 1 (Icam1), located on Chr 9, 7cM, contains 7 exons and is one of the immunoglobulin superfamily that binds to the integrins LFA-1 and Mac-1 on leukocytes. A neomycin resistance cassette was inserted into exon 4 (one of the 5 Ig-like extracellular domains) of the Icam1 gene. J1 ES cell clone 74 was injected into B6 blastocysts. This Icam1 mutation has been backcrossed to NOD for at least 6 generations (Martin et al, 2001). The Type 1 Diabetes Resource received Stock No. 005983 - NOD.129S4(B6)-Icam1tm1Jcgr/SmtnJ in 2005. Shortly after arriving at The Jackson Laboratory additional genetic quality control indicated C57BL/6 genomic contamination on Chr 6 and 7. Markers D6Mit178, D6Mit52 and D6Mit135 on Chr 6 were segregating for both NOD and C57BL/6. Through selective breeding the Chr. 6 markers are NOD in origin. Markers D7Mit139, D7Mit96 and D7Mit328 on Chr 7 were found to be of C57BL/6 origin. After 2 crosses to NOD/ShiLtJ, all three Chr 7 markers were homozygous for the NOD-derived alleles. In 2007, this strain is at generation N?+N2F2.
Gene & Allele Details
| Allele Symbol | Icam1tm1Jcgr | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Jose Carlos Gutierrez Ramos | ||
| Common Name(s) | ICAM-1-; ICAM-1-deficient; ICAMtm1-jcgr; | ||
| Mutation Made By | Jose-Carlos Gutierrez-Ramos, Millenium Pharmaceuticals | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Icam1, intercellular adhesion molecule 1 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | BB2; CD54; ICAM; Icam-1; Ly-47; MALA-2; MGC:6195; P3.58; lymphocyte antigen 47; | ||
| Molecular Note | A neomycin resistance cassette was inserted into exon 4 of the gene. While King, et. al. (J:25530) reported the potential for the production of functional proteins arising from alternative transcripts, corresponding signals were undetected by immunohistochemical analysis of homozygous mutant mice. [MGI Ref ID J:18743] | ||
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Icam1tm1Jcgr
Related Strains
Strains carrying Icam1tm1Jcgr allele
002867 B6.129S4-Icam1tm1Jcgr/J 005983 NOD.129S4(B6)-Icam1tm1Jcgr/SmtnJ View Strains carrying Icam1tm1Jcgr (2 strains)
002285 B6.129S7-Icam1tm1Bay Selptm1Bay/J 002127 B6.129S7-Icam1tm1Bay/J
Additional Web Information
Congenic Nomenclature
Research Applications
This mouse can be used to support research in many areas including:
Icam1tm1Jcgr relatedImmunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
Internal/Organ Research
Wound Healing (delayed/impaired)
References
Selected Reference(s)
Additional ReferencesMartin S; van Den Engel NK; Vinke A; Heidenthal E; Schulte B; Kolb H. 2001. Dominant role of intercellular adhesion molecule-1 in the pathogenesis of autoimmune diabetes in non-obese diabetic mice. J Autoimmun 17(2):109-17. [PubMed: 11591119] [MGI Ref ID J:71827]
Price and Supply Information
| Strain Name: | NOD.129S4(B6)-Icam1tm1Jcgr/J |
| Stock Number: | 006351 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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