Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System See Colony Maintenance Species laboratory mouse Generation N5+F4 (28-JAN-09)   Donating Investigator Richard Cummings,   University of Oklahoma

Description
Mice that are homozygous for both of the targeted mutations are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (protein) from either gene is detected by immunohistological analysis of skin from 16.5 day (post coitum) embyros. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and functions.

This strain was transferred from the collection of the Consortium for Functional Glycomics.

Development
For the Lgals1 targeted mutation allele, a targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 1.0kb of sequence that includes exon 2. The construct was electroporated into 129S6/SvEvTac derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into outbred MF-1 blastocysts. The resulting chimeric male animals were crossed to outbred MF-1 female mice. Heterozygotes were crossed to generate homozygotes.
For the Lgals3 targeted mutation allele, a targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 3.7kb of sequence that includes exons 2, 3 and 4. The construct was electroporated into 129/Sv and C57BL/6J and SJL derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into outbred MF-1 blastocysts. The resulting chimeric male animals were crossed to 129 female mice. Heterozygotes were crossed to generate homozygotes.
The single targeted mutation lines were subsequently crossed to generate this double mutant. The double mutant strain was then backcrossed to C57BL/6 for 5 generations prior to arrival at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Lgals1^tm1Rob allele

006337

B6.Cg-Lgals1tm1Rob/J

View Strains carrying   Lgals1^tm1Rob     (1 strain)

Strains carrying   Lgals3^tm1Poi allele

006338

B6.Cg-Lgals3tm1Poi/J

View Strains carrying   Lgals3^tm1Poi     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Lgals1^tm1Rob/Lgals1^tm1Rob Lgals3^tm1Poi/Lgals3^tm1Poi

        involves: 129 * 129/Sv * 129S/SvEv * C57BL/6J * SJL

• life span-post-weaning/aging
• *normal* life span-post-weaning/aging (MGI Ref ID J:47227)
  • mice exhibit a normal lifespan
• reproductive system phenotype
• *normal* reproductive system phenotype (MGI Ref ID J:47227)
  • mice exhibit normal reproduction
• skin/coat/nails phenotype
• *normal* skin/coat/nails phenotype (MGI Ref ID J:47227)
  • mice exhibit normal skin structure

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing

Genes & Alleles

Gene & Allele Information

Allele Symbol		Lgals1tm1Rob
Allele Name		targeted mutation 1, Elizabeth J Robertson
Allele Type		Targeted (knock-out)
Common Name(s)		gal-1-; gal1-;
Mutation Made By		Richard Cummings,   University of Oklahoma
Strain of Origin		129S/SvEv-Gpi1
ES Cell Line Name		CCE/EK.CCE
ES Cell Line Strain		129S/SvEv-Gpi1
Gene Symbol and Name		Lgals1, lectin, galactose binding, soluble 1
Chromosome		15
Gene Common Name(s)		AA410090; DKFZp686E23103; GAL1; GBP; Gal-1; Galbp; L14; Lect14; beta galactoside binding protein; expressed sequence AA410090; galectin-1; lactose binding soluble lectin, 14 kDa;
Molecular Note		Exon 2, encoding the carbohydrate-binding domain, was replaced with a neomycin selection cassette. Western blot analysis and immunostaining of sections showed an absence of encoded protein in muscle tissue obtained from adult homozygous mutant mice. [MGI Ref ID J:16245]
Allele Symbol		Lgals3tm1Poi
Allele Name		targeted mutation 1, Francoise Poirier
Allele Type		Targeted (knock-out)
Common Name(s)		GaL3-; galectin 3<->;
Mutation Made By		Richard Cummings,   University of Oklahoma
Strain of Origin		STOCK 129/Sv and C57BL/6J and SJL
ES Cell Line Name		WW6
ES Cell Line Strain		STOCK 129/Sv and C57BL/6J and SJL
Gene Symbol and Name		Lgals3, lectin, galactose binding, soluble 3
Chromosome		14
Gene Common Name(s)		CBP35; GAL3; GALBP; GALIG; L-34; LGALS2; MAC2; MGC105387; Mac-2; gal-3; galectin-3;
Molecular Note		3.7kb of sequence, encompassing exons 2 through 4, was replaced via the insertion of a neomycin selection cassette. Immunostaining of fetal skin showed an absence of protein in homozygous mutant embryos. [MGI Ref ID J:47227]

Genotyping

Genotyping Information

Genotyping Protocols

Lgals3^tm1Poi, Standard PCR
Lgals1^tm1Rob, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Colnot C; Fowlis D; Ripoche MA; Bouchaert I; Poirier F. 1998. Embryonic implantation in galectin 1/galectin 3 double mutant mice. Dev Dyn 211(4):306-13. [PubMed: 9566950]  [MGI Ref ID J:47227]

Poirier F; Robertson EJ. 1993. Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin. Development 119(4):1229-36. [PubMed: 8306885]  [MGI Ref ID J:16245]

Additional References

Lgals1^tm1Rob related

Barrionuevo P; Beigier-Bompadre M; Ilarregui JM; Toscano MA; Bianco GA; Isturiz MA; Rabinovich GA. 2007. A novel function for galectin-1 at the crossroad of innate and adaptive immunity: galectin-1 regulates monocyte/macrophage physiology through a nonapoptotic ERK-dependent pathway. J Immunol 178(1):436-45. [PubMed: 17182582]  [MGI Ref ID J:141926]

Blois SM; Ilarregui JM; Tometten M; Garcia M; Orsal AS; Cordo-Russo R; Toscano MA; Bianco GA; Kobelt P; Handjiski B; Tirado I; Markert UR; Klapp BF; Poirier F; Szekeres-Bartho J; Rabinovich GA; Arck PC. 2007. A pivotal role for galectin-1 in fetomaternal tolerance. Nat Med 13(12):1450-7. [PubMed: 18026113]  [MGI Ref ID J:130208]

Cao Z; Said N; Amin S; Wu HK; Bruce A; Garate M; Hsu DK; Kuwabara I; Liu FT; Panjwani N. 2002. Galectins-3 and -7, but not galectin-1, play a role in re-epithelialization of wounds. J Biol Chem 277(44):42299-305. [PubMed: 12194966]  [MGI Ref ID J:79889]

Case D; Irwin D; Ivester C; Harral J; Morris K; Imamura M; Roedersheimer M; Patterson A; Carr M; Hagen M; Saavedra M; Crossno J Jr; Young KA; Dempsey EC; Poirier F; West J; Majka S. 2007. Mice deficient in galectin-1 exhibit attenuated physiological responses to chronic hypoxia-induced pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 292(1):L154-64. [PubMed: 16951131]  [MGI Ref ID J:141598]

Colnot C; Ripoche MA; Scaerou F; Foulis D; Poirier F. 1996. Galectins in mouse embryogenesis. Biochem Soc Trans 24(1):141-6. [PubMed: 8674632]  [MGI Ref ID J:31899]

Cooper D; Norling LV; Perretti M. 2008. Novel insights into the inhibitory effects of Galectin-1 on neutrophil recruitment under flow. J Leukoc Biol 83(6):1459-66. [PubMed: 18372340]  [MGI Ref ID J:136833]

Espeli M; Mancini SJ; Breton C; Poirier F; Schiff C. 2009. Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions. Blood 113(23):5878-86. [PubMed: 19329777]  [MGI Ref ID J:149365]

Garin MI; Chu CC; Golshayan D; Cernuda-Morollon E; Wait R; Lechler RI. 2007. Galectin-1: a key effector of regulation mediated by CD4+CD25+ T cells. Blood 109(5):2058-65. [PubMed: 17110462]  [MGI Ref ID J:145355]

Georgiadis V; Stewart HJ; Pollard HJ; Tavsanoglu Y; Prasad R; Horwood J; Deltour L; Goldring K; Poirier F; Lawrence-Watt DJ. 2007. Lack of galectin-1 results in defects in myoblast fusion and muscle regeneration. Dev Dyn 236(4):1014-1024. [PubMed: 17366633]  [MGI Ref ID J:119480]

Ilarregui JM; Croci DO; Bianco GA; Toscano MA; Salatino M; Vermeulen ME; Geffner JR; Rabinovich GA. 2009. Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10. Nat Immunol 10(9):981-91. [PubMed: 19668220]  [MGI Ref ID J:151758]

Jouault T; El Abed-El Behi M; Martinez-Esparza M; Breuilh L; Trinel PA; Chamaillard M; Trottein F; Poulain D. 2006. Specific recognition of Candida albicans by macrophages requires galectin-3 to discriminate Saccharomyces cerevisiae and needs association with TLR2 for signaling. J Immunol 177(7):4679-87. [PubMed: 16982907]  [MGI Ref ID J:139314]

Liu SD; Tomassian T; Bruhn KW; Miller JF; Poirier F; Miceli MC. 2009. Galectin-1 tunes TCR binding and signal transduction to regulate CD8 burst size. J Immunol 182(9):5283-95. [PubMed: 19380775]  [MGI Ref ID J:147951]

Liu SD; Whiting CC; Tomassian T; Pang M; Bissel SJ; Baum LG; Mossine VV; Poirier F; Huflejt ME; Miceli MC. 2008. Endogenous galectin-1 enforces class I-restricted TCR functional fate decisions in thymocytes. Blood 112(1):120-30. [PubMed: 18323414]  [MGI Ref ID J:137307]

McGraw J; Gaudet AD; Oschipok LW; Steeves JD; Poirier F; Tetzlaff W; Ramer MS. 2005. Altered primary afferent anatomy and reduced thermal sensitivity in mice lacking galectin-1. Pain 114(1-2):7-18. [PubMed: 15733626]  [MGI Ref ID J:101850]

McGraw J; McPhail LT; Oschipok LW; Horie H; Poirier F; Steeves JD; Ramer MS; Tetzlaff W. 2004. Galectin-1 in regenerating motoneurons. Eur J Neurosci 20(11):2872-80. [PubMed: 15579141]  [MGI Ref ID J:101280]

Moore GT; Brown SJ; Winterhalter AC; Lust M; Salvaris EJ; Selan C; Nandurkar HH; Desmond PV; Cowan PJ; d'Apice AJ. 2008. Glycosylation changes in hFUT1 transgenic mice increase TCR signaling and apoptosis resulting in thymocyte maturation arrest. Mol Immunol 45(8):2401-10. [PubMed: 18155296]  [MGI Ref ID J:138374]

Motran CC; Molinder KM; Liu SD; Poirier F; Miceli MC. 2008. Galectin-1 functions as a Th2 cytokine that selectively induces Th1 apoptosis and promotes Th2 function. Eur J Immunol 38(11):3015-27. [PubMed: 18991278]  [MGI Ref ID J:141406]

Plachta N; Annaheim C; Bissiere S; Lin S; Ruegg M; Hoving S; Muller D; Poirier F; Bibel M; Barde YA. 2007. Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nat Neurosci 10(6):712-9. [PubMed: 17486104]  [MGI Ref ID J:122104]

Puche AC; Poirier F; Hair M; Bartlett PF; Key B. 1996. Role of galectin-1 in the developing mouse olfactory system. Dev Biol 179(1):274-87. [PubMed: 8873770]  [MGI Ref ID J:36694]

Sakaguchi M; Shingo T; Shimazaki T; Okano HJ; Shiwa M; Ishibashi S; Oguro H; Ninomiya M; Kadoya T; Horie H; Shibuya A; Mizusawa H; Poirier F; Nakauchi H; Sawamoto K; Okano H. 2006. A carbohydrate-binding protein, Galectin-1, promotes proliferation of adult neural stem cells. Proc Natl Acad Sci U S A 103(18):7112-7. [PubMed: 16636291]  [MGI Ref ID J:109590]

Shoji H; Deltour L; Nakamura T; Tajbakhsh S; Poirier F. 2009. Expression pattern and role of Galectin1 during early mouse myogenesis. Dev Growth Differ 51(7):607-15. [PubMed: 19712265]  [MGI Ref ID J:153078]

Thijssen VL; Postel R; Brandwijk RJ; Dings RP; Nesmelova I; Satijn S; Verhofstad N; Nakabeppu Y; Baum LG; Bakkers J; Mayo KH; Poirier F; Griffioen AW. 2006. Galectin-1 is essential in tumor angiogenesis and is a target for antiangiogenesis therapy. Proc Natl Acad Sci U S A 103(43):15975-80. [PubMed: 17043243]  [MGI Ref ID J:147258]

Toscano MA; Bianco GA; Ilarregui JM; Croci DO; Correale J; Hernandez JD; Zwirner NW; Poirier F; Riley EM; Baum LG; Rabinovich GA. 2007. Differential glycosylation of T(H)1, T(H)2 and T(H)-17 effector cells selectively regulates susceptibility to cell death. Nat Immunol 8(8):825-834. [PubMed: 17589510]  [MGI Ref ID J:123416]

Lgals3^tm1Poi related

Breuilh L; Vanhoutte F; Fontaine J; van Stijn CM; Tillie-Leblond I; Capron M; Faveeuw C; Jouault T; van Die I; Gosset P; Trottein F. 2007. Galectin-3 modulates immune and inflammatory responses during helminthic infection: impact of galectin-3 deficiency on the functions of dendritic cells. Infect Immun 75(11):5148-57. [PubMed: 17785480]  [MGI Ref ID J:126353]

Chiu MG; Johnson TM; Woolf AS; Dahm-Vicker EM; Long DA; Guay-Woodford L; Hillman KA; Bawumia S; Venner K; Hughes RC; Poirier F; Winyard PJ. 2006. Galectin-3 associates with the primary cilium and modulates cyst growth in congenital polycystic kidney disease. Am J Pathol 169(6):1925-38. [PubMed: 17148658]  [MGI Ref ID J:116210]

Colnot C; Ripoche MA; Milon G; Montagutelli X; Crocker PR; Poirier F. 1998. Maintenance of granulocyte numbers during acute peritonitis is defective in galectin-3-null mutant mice. Immunology 94(3):290-6. [PubMed: 9767409]  [MGI Ref ID J:110422]

Colnot C; Ripoche MA; Scaerou F; Foulis D; Poirier F. 1996. Galectins in mouse embryogenesis. Biochem Soc Trans 24(1):141-6. [PubMed: 8674632]  [MGI Ref ID J:31899]

Colnot C; Sidhu SS; Balmain N; Poirier F. 2001. Uncoupling of chondrocyte death and vascular invasion in mouse galectin 3 null mutant bones. Dev Biol 229(1):203-14. [PubMed: 11133164]  [MGI Ref ID J:66902]

Delacour D; Koch A; Ackermann W; Eude-Le Parco I; Elsasser HP; Poirier F; Jacob R. 2008. Loss of galectin-3 impairs membrane polarisation of mouse enterocytes in vivo. J Cell Sci 121(Pt 4):458-65. [PubMed: 18211959]  [MGI Ref ID J:138282]

Farnworth SL; Henderson NC; Mackinnon AC; Atkinson KM; Wilkinson T; Dhaliwal K; Hayashi K; Simpson AJ; Rossi AG; Haslett C; Sethi T. 2008. Galectin-3 reduces the severity of pneumococcal pneumonia by augmenting neutrophil function. Am J Pathol 172(2):395-405. [PubMed: 18202191]  [MGI Ref ID J:131407]

Henderson NC; Mackinnon AC; Farnworth SL; Kipari T; Haslett C; Iredale JP; Liu FT; Hughes J; Sethi T. 2008. Galectin-3 expression and secretion links macrophages to the promotion of renal fibrosis. Am J Pathol 172(2):288-98. [PubMed: 18202187]  [MGI Ref ID J:131371]

Henderson NC; Mackinnon AC; Farnworth SL; Poirier F; Russo FP; Iredale JP; Haslett C; Simpson KJ; Sethi T. 2006. Galectin-3 regulates myofibroblast activation and hepatic fibrosis. Proc Natl Acad Sci U S A 103(13):5060-5. [PubMed: 16549783]  [MGI Ref ID J:107656]

MacKinnon AC; Farnworth SL; Hodkinson PS; Henderson NC; Atkinson KM; Leffler H; Nilsson UJ; Haslett C; Forbes SJ; Sethi T. 2008. Regulation of alternative macrophage activation by galectin-3. J Immunol 180(4):2650-8. [PubMed: 18250477]  [MGI Ref ID J:131971]

Nachtigal M; Ghaffar A; Mayer EP. 2008. Galectin-3 gene inactivation reduces atherosclerotic lesions and adventitial inflammation in ApoE-deficient mice. Am J Pathol 172(1):247-55. [PubMed: 18156214]  [MGI Ref ID J:130923]

Oakley MS; Majam V; Mahajan B; Gerald N; Anantharaman V; Ward JM; Faucette LJ; McCutchan TF; Zheng H; Terabe M; Berzofsky JA; Aravind L; Kumar S. 2009. Pathogenic roles of CD14, galectin-3, and OX40 during experimental cerebral malaria in mice. PLoS One 4(8):e6793. [PubMed: 19710907]  [MGI Ref ID J:152399]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes for both targeted mutations.
Mating System	See above
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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