Strain Name: |
B6.Cg-Lgals3tm1Poi Lgals1tm1Rob/J |
|---|---|
Stock Number: |
006354 |
Availability: | Repository- Live |
General Terms and Conditions |
| Strain Common Names | gal1-/-; gal3-/-; galectin 1/galectin 3 double mutant; |
| Genes & Alleles | Lgals1; Lgals1tm1Rob; Lgals3; Lgals3tm1Poi; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System See Colony Maintenance (Female x Male) Species laboratory mouse Donating Investigator Richard Cummings, University of Oklahoma Generation N5+F1 (04-DEC-07) Strain Description
Mice that are homozygous for both of the targeted mutations are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (protein) from either gene is detected by immunohistological analysis of skin from 16.5 day (post coitum) embyros. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and functions.This strain was transferred from the collection of the Consortium for Functional Glycomics.
Strain Development
For the Lgals1 targeted mutation allele, a targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 1.0kb of sequence that includes exon 2. The construct was electroporated into 129S6/SvEvTac derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into outbred MF-1 blastocysts. The resulting chimeric male animals were crossed to outbred MF-1 female mice. Heterozygotes were crossed to generate homozygotes.
For the Lgals3 targeted mutation allele, a targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt 3.7kb of sequence that includes exons 2, 3 and 4. The construct was electroporated into 129/Sv and C57BL/6J and SJL derived WW6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into outbred MF-1 blastocysts. The resulting chimeric male animals were crossed to 129 female mice. Heterozygotes were crossed to generate homozygotes.
The single targeted mutation lines were subsequently crossed to generate this double mutant. The double mutant strain was then backcrossed to C57BL/6 for 5 generations prior to arrival at The Jackson Laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Lgals1tm1Rob | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Elizabeth J Robertson | ||
| Common Name(s) | gal-1-; gal1-; | ||
| Mutation Made By | Richard Cummings, University of Oklahoma | ||
| Strain of Origin | 129S/SvEv-Gpi1 | ||
| ES Cell Line Name | CCE/EK.CCE | ||
| ES Cell Line Strain | 129S/SvEv-Gpi1 | ||
| Gene Symbol and Name | Lgals1, lectin, galactose binding, soluble 1 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | AA410090; DKFZp686E23103; GAL1; GBP; Gal-1; Galbp; L14; Lect14; beta galactoside binding protein; expressed sequence AA410090; galectin-1; lactose binding soluble lectin, 14 kDa; | ||
| Molecular Note | Exon 2, encoding the carbohydrate-binding domain, was deleted by the insertion of neomycin selection cassette. Western blot analysis and immunostaining of sections showed an absence of encoded protein in muscle tissue obtained from adult homozygous mutant mice. [MGI Ref ID J:16245] | ||
| Allele Symbol | Lgals3tm1Poi | ||
| Allele Name | targeted mutation 1, Francoise Poirier | ||
| Common Name(s) | GaL3-; galectin 3<->; | ||
| Mutation Made By | Richard Cummings, University of Oklahoma | ||
| Strain of Origin | STOCK 129/Sv and C57BL/6J and SJL | ||
| ES Cell Line Name | WW6 | ||
| ES Cell Line Strain | STOCK 129/Sv and C57BL/6J and SJL | ||
| Gene Symbol and Name | Lgals3, lectin, galactose binding, soluble 3 | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | CBP35; GAL3; GALBP; GALIG; L-34; LGALS2; MAC2; MGC105387; Mac-2; gal-3; galectin-3; | ||
| Molecular Note | 3.7kb of sequence, encompassing exons 2 through 4, was replaced via the insertion of a neomycin selection cassette. Immunostaining of fetal skin showed an absence of protein in homozygous mutant embryos. [MGI Ref ID J:47227] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Lgals1tm1Rob
Lgals3tm1Poi
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes for both targeted mutations. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Lgals1tm1Rob allele
006337 B6.Cg-Lgals1tm1Rob/J View Strains carrying Lgals1tm1Rob (1 strain)
006338 B6.Cg-Lgals3tm1Poi/J
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Protein Processing
References
Selected Reference(s)
Additional ReferencesColnot C; Fowlis D; Ripoche MA; Bouchaert I; Poirier F. 1998. Embryonic implantation in galectin 1/galectin 3 double mutant mice. Dev Dyn 211(4):306-13. [PubMed: 9566950] [MGI Ref ID J:47227]
Poirier F; Robertson EJ. 1993. Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin. Development 119(4):1229-36. [PubMed: 8306885] [MGI Ref ID J:16245]
Price and Supply Information
| Strain Name: | B6.Cg-Lgals3tm1Poi Lgals1tm1Rob/J |
| Stock Number: | 006354 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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