Strain Name:

NOD.129S2(B6)-Airetm1.1Doi/DoiJ

Stock Number:

006360

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Availability:

Cryopreserved - Ready for recovery

Common Names: NOD.Aire-/-;    
Autoimmune regulator knockout mice may be useful in studies related autoimmune disease and tolerance induction.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain NOD
Donor Strain B6.129S2/SvPas
H2 Haplotypeg7
GenerationN12F5pN1
Generation Definitions
 
Donating Investigator Christophe Benoist,   Joslin Diabetes Center

Appearance
pink-eyed, albino
Related Genotype: A/A Tyrc/Tyrc

Description
Autoimmune regulator, Aire, located on Chromosome 10, 41.6cm controls, in part, ectopic gene expression involved with tolerance induction, and is specifically involved with autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy in humans. Exon 2 and portions of the flanking introns of the Aire gene were deleted by Cre-lox-mediated homologous recombination. Analysis of the mutated Aire transcript identified a frameshift that precludes translation past exon 1. The Aire mutation originated on a 129B6F2 background and was backcrossed to NOD for 12 generations. NOD.Aire deficient mice are normoglycemic, but experience weight loss between 5 and 15 weeks of age. Approximately 79% die spontaneously or must be euthanized due to declining health at between 6 and 14 weeks of age. Pathology indicates lung lesions with generalized pneumonitis, severe exocrine (not endocrine) pancreatitis and lymphocytic infiltration in the liver, salivary, stomach, reproductive systems, and the presence of circulating autoantibodies against ovary, stomach, retina and exocrine pancreas.

This stock is useful in the study of Aire function in autoimmunity and tolerance induction.

Development
To create this mutant the originating investigator employed a targeting vector bearing a loxP site-flanked Pgk-neo cassette inserted into intron 2. An additional single loxP site was placed in intron 1. The targeting construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were mated with CMV-cre transgenic mice on a C57BL/6 background. Exon 2 and portions of introns 1 and 3 were excised in the offspring. The C57BL/6 Aire deficient stock was then backcrossed to NOD for 12 generations. In 2007, the T1DR received this strain at generation N12F5.

Control Information

  Control
   Wild-type from the colony
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Airetm1.1Doi allele
004743   B6.129S2-Airetm1.1Doi/J
View Strains carrying   Airetm1.1Doi     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Autoimmune Polyendocrine Syndrome, Type I, with or without Reversible Metaphyseal Dysplasia; APS1
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Airetm1.1Doi/Airetm1.1Doi

        NOD.129S2(B6)-Airetm1.1Doi/Doi
  • mortality/aging
  • premature death
    • 79% of mice die between 6 and 14 weeks of age with the likely cause of death being inflammatory lesions of the lungs with generalized pneumoitis   (MGI Ref ID J:107432)
    • over 95% of the mice die by 20 weeks of age   (MGI Ref ID J:107432)
  • growth/size/body phenotype
  • cachexia
    • mice are prone to losing weight between 5 and 15 weeks of age with weight loss correlating to the intensity of pancreas or lung inflammation   (MGI Ref ID J:107432)
  • immune system phenotype
  • increased autoantibody level
    • mice generate autoantibodies with a reactivity that mirror the histopathology associated with this mouse strain   (MGI Ref ID J:107432)
    • the autoantibodies generated on the NOD background target numerous tissues including the pancreas   (MGI Ref ID J:107432)
    • the pancreas is not targeted by autoantibodies when the mutant allele is present on other genetic backgrounds   (MGI Ref ID J:107432)
  • increased incidence of corneal inflammation
    • cornea inflammation occurs in 14% of mice of this background by 20 weeks of age but is not observed when the mutant allele is when the mutant allele is on the SJL or C57BL/6 background   (MGI Ref ID J:107432)
  • liver inflammation
    • liver inflammation is observed in 86% of mice by 20 weeks of age while no inflammation is present when the mutant allele is on the C57BL/6 background   (MGI Ref ID J:107432)
  • lung inflammation
    • lung inflammation is observed in 100% of mice by 20 weeks of age which is a much higher incidence than observed when the mutant allele is on the C57BL/6 background   (MGI Ref ID J:107432)
    • lesions of the lung are severe enough to likely cause the runting and premature death associated with this strain   (MGI Ref ID J:107432)
  • ovary inflammation
    • 88% of mice exhibit inflammation of the ovaries on this background while no inflammation is observed in C57BL/6 mice carrying the mutant allele   (MGI Ref ID J:107432)
  • pancreas inflammation
    • all mice exhibit inflammation of the pancreas on this background while no inflammation is observed in C57BL/6 or BALB/c mice carrying this mutant allele   (MGI Ref ID J:107432)
    • the lesions are highly localized within the pancreas and appear to progress from an initial extravasation into the perivascular connective tissue to a front of cell destruction that moves through the exocrine lobe   (MGI Ref ID J:107432)
    • islet cells are spared from destruction and the mice remain normoglycemic through 20 weeks of age   (MGI Ref ID J:107432)
  • prostate gland inflammation
    • all mice have inflammation of the prostate by 20 weeks of age   (MGI Ref ID J:107432)
  • salivary gland inflammation
    • sialitis is observed in all mice by 20 weeks of age which is a much higher incidence than what is observed when the mutant allele is on the C57BL/6 or BALB/c backgrounds   (MGI Ref ID J:107432)
  • stomach inflammation
    • gastritis occurs in 87% of mice by 20 weeks of age but is rarely observed when the mutant allele is on C57BL/6   (MGI Ref ID J:107432)
  • thyroid inflammation
    • inflammation of the thyroid occurs in 54% of mice on the NOD background but is not observed in when the mutant allele is present on C57BL/6, BALB/c, or SJL backgrounds   (MGI Ref ID J:107432)
  • vision/eye phenotype
  • increased incidence of corneal inflammation
    • cornea inflammation occurs in 14% of mice of this background by 20 weeks of age but is not observed when the mutant allele is when the mutant allele is on the SJL or C57BL/6 background   (MGI Ref ID J:107432)
  • retinal degeneration
    • retinal degeneration resulting from autoimmune disease occurs by 20 weeks of age in 93% of mice   (MGI Ref ID J:107432)
  • endocrine/exocrine gland phenotype
  • pancreas inflammation
    • all mice exhibit inflammation of the pancreas on this background while no inflammation is observed in C57BL/6 or BALB/c mice carrying this mutant allele   (MGI Ref ID J:107432)
    • the lesions are highly localized within the pancreas and appear to progress from an initial extravasation into the perivascular connective tissue to a front of cell destruction that moves through the exocrine lobe   (MGI Ref ID J:107432)
    • islet cells are spared from destruction and the mice remain normoglycemic through 20 weeks of age   (MGI Ref ID J:107432)
  • prostate gland inflammation
    • all mice have inflammation of the prostate by 20 weeks of age   (MGI Ref ID J:107432)
  • salivary gland inflammation
    • sialitis is observed in all mice by 20 weeks of age which is a much higher incidence than what is observed when the mutant allele is on the C57BL/6 or BALB/c backgrounds   (MGI Ref ID J:107432)
  • thyroid inflammation
    • inflammation of the thyroid occurs in 54% of mice on the NOD background but is not observed in when the mutant allele is present on C57BL/6, BALB/c, or SJL backgrounds   (MGI Ref ID J:107432)
  • liver/biliary system phenotype
  • liver inflammation
    • liver inflammation is observed in 86% of mice by 20 weeks of age while no inflammation is present when the mutant allele is on the C57BL/6 background   (MGI Ref ID J:107432)
  • reproductive system phenotype
  • ovary inflammation
    • 88% of mice exhibit inflammation of the ovaries on this background while no inflammation is observed in C57BL/6 mice carrying the mutant allele   (MGI Ref ID J:107432)
  • prostate gland inflammation
    • all mice have inflammation of the prostate by 20 weeks of age   (MGI Ref ID J:107432)
  • respiratory system phenotype
  • lung inflammation
    • lung inflammation is observed in 100% of mice by 20 weeks of age which is a much higher incidence than observed when the mutant allele is on the C57BL/6 background   (MGI Ref ID J:107432)
    • lesions of the lung are severe enough to likely cause the runting and premature death associated with this strain   (MGI Ref ID J:107432)
  • digestive/alimentary phenotype
  • salivary gland inflammation
    • sialitis is observed in all mice by 20 weeks of age which is a much higher incidence than what is observed when the mutant allele is on the C57BL/6 or BALB/c backgrounds   (MGI Ref ID J:107432)
  • stomach inflammation
    • gastritis occurs in 87% of mice by 20 weeks of age but is rarely observed when the mutant allele is on C57BL/6   (MGI Ref ID J:107432)

Airetm1.1Doi/Airetm1.1Doi

        NOD.129S2-Airetm1.1Doi
  • immune system phenotype
  • abnormal CD4-positive, alpha-beta T cell physiology
    • there is increased percentage of CD4 T cells in the spleen that are activated based on surface marker expression (CD62Llo CD44hi)   (MGI Ref ID J:135154)
  • chronic pancreas inflammation
    • mice 10 weeks have age have severe lymphocytic infiltration of the pancreas   (MGI Ref ID J:135154)
  • insulitis
    • mice 25 weeks of age have intra-islet infiltration of lymphocytes   (MGI Ref ID J:135154)
  • lacrimal gland inflammation
    • mice 10 weeks of age have severe lymphocytic infiltration of the lacrimal glands   (MGI Ref ID J:135154)
  • lung inflammation
    • lymphocyte infiltrates are found in mice by 10 weeks of age   (MGI Ref ID J:135154)
  • salivary gland inflammation
    • mice 10 weeks of age have severe lymphocytic infiltration of the salivary glands   (MGI Ref ID J:135154)
  • stomach inflammation
    • occurs in mice by 10 weeks of age   (MGI Ref ID J:135154)
  • thyroid inflammation
    • severe thyroiditis is observed in mice 15 to 25 weeks in age   (MGI Ref ID J:135154)
  • uveitis
    • retinal lymphocytic infiltration occurs in the eye   (MGI Ref ID J:135154)
  • endocrine/exocrine gland phenotype
  • chronic pancreas inflammation
    • mice 10 weeks have age have severe lymphocytic infiltration of the pancreas   (MGI Ref ID J:135154)
  • insulitis
    • mice 25 weeks of age have intra-islet infiltration of lymphocytes   (MGI Ref ID J:135154)
  • lacrimal gland inflammation
    • mice 10 weeks of age have severe lymphocytic infiltration of the lacrimal glands   (MGI Ref ID J:135154)
  • salivary gland inflammation
    • mice 10 weeks of age have severe lymphocytic infiltration of the salivary glands   (MGI Ref ID J:135154)
  • thyroid inflammation
    • severe thyroiditis is observed in mice 15 to 25 weeks in age   (MGI Ref ID J:135154)
  • respiratory system phenotype
  • lung inflammation
    • lymphocyte infiltrates are found in mice by 10 weeks of age   (MGI Ref ID J:135154)
  • vision/eye phenotype
  • lacrimal gland inflammation
    • mice 10 weeks of age have severe lymphocytic infiltration of the lacrimal glands   (MGI Ref ID J:135154)
  • uveitis
    • retinal lymphocytic infiltration occurs in the eye   (MGI Ref ID J:135154)
  • digestive/alimentary phenotype
  • salivary gland inflammation
    • mice 10 weeks of age have severe lymphocytic infiltration of the salivary glands   (MGI Ref ID J:135154)
  • stomach inflammation
    • occurs in mice by 10 weeks of age   (MGI Ref ID J:135154)
  • hematopoietic system phenotype
  • abnormal CD4-positive, alpha-beta T cell physiology
    • there is increased percentage of CD4 T cells in the spleen that are activated based on surface marker expression (CD62Llo CD44hi)   (MGI Ref ID J:135154)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      autoimmune pancreatitis and sialoadenitis
      autoimmune polyendocrinopathy candidiasis ectodermal dystrophy

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Airetm1.1Doi
Allele Name targeted mutation 1.1, Christophe Benoist and Diane Mathis
Allele Type Targeted (Null/Knockout)
Common Name(s) Aire-; Aireo; HD Aire-;
Mutation Made By Diane Mathis,   Harvard Medical School
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Aire, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)
Chromosome 10
Gene Common Name(s) AIRE1; APECED; APS1; APSI; PGA1;
Molecular Note The floxed fragment containing exon 2, portions of the surrounding introns, and a neo cassette were excised via cre mediated recombination in vivo. While shortened transcript was detected by RT-PCR analysis of homozygous mutant thymii, sequence analysis of the transcript identified a frameshift mutation resulting from the splicing of exons 1 and 3 that precludes translation past exon 1. [MGI Ref ID J:83422]

Genotyping

Genotyping Information

Genotyping Protocols

Airetm1.1Doi,

SEPARATED MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Airetm1.1Doi related

Anderson MS; Venanzi ES; Chen Z; Berzins SP; Benoist C; Mathis D. 2005. The cellular mechanism of Aire control of T cell tolerance. Immunity 23(2):227-39. [PubMed: 16111640]  [MGI Ref ID J:100515]

Anderson MS; Venanzi ES; Klein L; Chen Z; Berzins SP; Turley SJ; von Boehmer H; Bronson R; Dierich A; Benoist C; Mathis D. 2002. Projection of an immunological self shadow within the thymus by the aire protein. Science 298(5597):1395-401. [PubMed: 12376594]  [MGI Ref ID J:83422]

Chen J; Qian H; Horai R; Chan CC; Falick Y; Caspi RR. 2013. Comparative analysis of induced vs. spontaneous models of autoimmune uveitis targeting the interphotoreceptor retinoid binding protein. PLoS One 8(8):e72161. [PubMed: 24015215]  [MGI Ref ID J:206410]

Chen YF; Zhou D; Metzger T; Gallup M; Jeanne M; Gould DB; Anderson MS; McNamara NA. 2014. Spontaneous development of autoimmune uveitis Is CCR2 dependent. Am J Pathol 184(6):1695-705. [PubMed: 24736166]  [MGI Ref ID J:211671]

Chen YT; Chen FY; Vijmasi T; Stephens DN; Gallup M; McNamara NA. 2013. Pax6 downregulation mediates abnormal lineage commitment of the ocular surface epithelium in aqueous-deficient dry eye disease. PLoS One 8(10):e77286. [PubMed: 24143217]  [MGI Ref ID J:209118]

Chen YT; Lazarev S; Bahrami AF; Noble LB; Chen FY; Zhou D; Gallup M; Yadav M; McNamara NA. 2012. Interleukin-1 receptor mediates the interplay between CD4(+) T cells and ocular resident cells to promote keratinizing squamous metaplasia in Sjogren's syndrome. Lab Invest 92(4):556-70. [PubMed: 22231738]  [MGI Ref ID J:181948]

Chen YT; Nikulina K; Lazarev S; Bahrami AF; Noble LB; Gallup M; McNamara NA. 2010. Interleukin-1 as a phenotypic immunomodulator in keratinizing squamous metaplasia of the ocular surface in Sjogren's syndrome. Am J Pathol 177(3):1333-43. [PubMed: 20696775]  [MGI Ref ID J:163691]

Chen Z; Benoist C; Mathis D. 2005. How defects in central tolerance impinge on a deficiency in regulatory T cells. Proc Natl Acad Sci U S A 102(41):14735-40. [PubMed: 16203996]  [MGI Ref ID J:102496]

Danso-Abeam D; Staats KA; Franckaert D; Van Den Bosch L; Liston A; Gray DH; Dooley J. 2013. Aire mediates thymic expression and tolerance of pancreatic antigens via an unconventional transcriptional mechanism. Eur J Immunol 43(1):75-84. [PubMed: 23041971]  [MGI Ref ID J:191104]

DeVoss J; Hou Y; Johannes K; Lu W; Liou GI; Rinn J; Chang H; Caspi RR; Fong L; Anderson MS. 2006. Spontaneous autoimmunity prevented by thymic expression of a single self-antigen. J Exp Med 203(12):2727-35. [PubMed: 17116738]  [MGI Ref ID J:124583]

DeVoss JJ; LeClair NP; Hou Y; Grewal NK; Johannes KP; Lu W; Yang T; Meagher C; Fong L; Strauss EC; Anderson MS. 2010. An autoimmune response to odorant binding protein 1a is associated with dry eye in the aire-deficient mouse. J Immunol 184(8):4236-46. [PubMed: 20237294]  [MGI Ref ID J:159869]

Devoss JJ; Shum AK; Johannes KP; Lu W; Krawisz AK; Wang P; Yang T; Leclair NP; Austin C; Strauss EC; Anderson MS. 2008. Effector mechanisms of the autoimmune syndrome in the murine model of autoimmune polyglandular syndrome type 1. J Immunol 181(6):4072-9. [PubMed: 18768863]  [MGI Ref ID J:139094]

Dooley J; Erickson M; Farr AG. 2008. Alterations of the medullary epithelial compartment in the Aire-deficient thymus: implications for programs of thymic epithelial differentiation. J Immunol 181(8):5225-32. [PubMed: 18832676]  [MGI Ref ID J:140094]

Gavanescu I; Benoist C; Mathis D. 2008. B cells are required for Aire-deficient mice to develop multi-organ autoinflammation: A therapeutic approach for APECED patients. Proc Natl Acad Sci U S A 105(35):13009-14. [PubMed: 18755889]  [MGI Ref ID J:139126]

Gavanescu I; Kessler B; Ploegh H; Benoist C; Mathis D. 2007. Loss of Aire-dependent thymic expression of a peripheral tissue antigen renders it a target of autoimmunity. Proc Natl Acad Sci U S A 104(11):4583-7. [PubMed: 17360567]  [MGI Ref ID J:119475]

Gillard GO; Dooley J; Erickson M; Peltonen L; Farr AG. 2007. Aire-dependent alterations in medullary thymic epithelium indicate a role for Aire in thymic epithelial differentiation. J Immunol 178(5):3007-15. [PubMed: 17312146]  [MGI Ref ID J:144101]

Giraud M; Yoshida H; Abramson J; Rahl PB; Young RA; Mathis D; Benoist C. 2012. Aire unleashes stalled RNA polymerase to induce ectopic gene expression in thymic epithelial cells. Proc Natl Acad Sci U S A 109(2):535-40. [PubMed: 22203960]  [MGI Ref ID J:179981]

Gray D; Abramson J; Benoist C; Mathis D. 2007. Proliferative arrest and rapid turnover of thymic epithelial cells expressing Aire. J Exp Med 204(11):2521-8. [PubMed: 17908938]  [MGI Ref ID J:126040]

Gray DH; Gavanescu I; Benoist C; Mathis D. 2007. Danger-free autoimmune disease in Aire-deficient mice. Proc Natl Acad Sci U S A 104(46):18193-8. [PubMed: 17991771]  [MGI Ref ID J:127308]

Guerau-de-Arellano M; Martinic M; Benoist C; Mathis D. 2009. Neonatal tolerance revisited: a perinatal window for Aire control of autoimmunity. J Exp Med 206(6):1245-52. [PubMed: 19487417]  [MGI Ref ID J:149510]

He Q; Morillon YM 2nd; Spidale NA; Kroger CJ; Liu B; Sartor RB; Wang B; Tisch R. 2013. Thymic development of autoreactive T cells in NOD mice is regulated in an age-dependent manner. J Immunol 191(12):5858-66. [PubMed: 24198282]  [MGI Ref ID J:207147]

Hou Y; DeVoss J; Dao V; Kwek S; Simko JP; McNeel DG; Anderson MS; Fong L. 2009. An aberrant prostate antigen-specific immune response causes prostatitis in mice and is associated with chronic prostatitis in humans. J Clin Invest 119(7):2031-41. [PubMed: 19603556]  [MGI Ref ID J:152543]

Jasti S; Warren BD; McGinnis LK; Kinsey WH; Petroff BK; Petroff MG. 2012. The autoimmune regulator prevents premature reproductive senescence in female mice. Biol Reprod 86(4):110. [PubMed: 22219212]  [MGI Ref ID J:185765]

Jiang W; Anderson MS; Bronson R; Mathis D; Benoist C. 2005. Modifier loci condition autoimmunity provoked by Aire deficiency. J Exp Med 202(6):805-15. [PubMed: 16172259]  [MGI Ref ID J:107432]

Koh AS; Kingston RE; Benoist C; Mathis D. 2010. Global relevance of Aire binding to hypomethylated lysine-4 of histone-3. Proc Natl Acad Sci U S A 107(29):13016-21. [PubMed: 20615959]  [MGI Ref ID J:162311]

Li S; Nikulina K; DeVoss J; Wu AJ; Strauss EC; Anderson MS; McNamara NA. 2008. Small proline-rich protein 1B (SPRR1B) is a biomarker for squamous metaplasia in dry eye disease. Invest Ophthalmol Vis Sci 49(1):34-41. [PubMed: 18172072]  [MGI Ref ID J:132504]

Misharin AV; Nagayama Y; Aliesky HA; Rapoport B; McLachlan SM. 2009. Studies in mice deficient for the autoimmune regulator (Aire) and transgenic for the thyrotropin receptor reveal a role for Aire in tolerance for thyroid autoantigens. Endocrinology 150(6):2948-56. [PubMed: 19264867]  [MGI Ref ID J:158187]

Schaller CE; Wang CL; Beck-Engeser G; Goss L; Scott HS; Anderson MS; Wabl M. 2008. Expression of Aire and the early wave of apoptosis in spermatogenesis. J Immunol 180(3):1338-43. [PubMed: 18209027]  [MGI Ref ID J:131354]

Shum AK; Alimohammadi M; Tan CL; Cheng MH; Metzger TC; Law CS; Lwin W; Perheentupa J; Bour-Jordan H; Carel JC; Husebye ES; De Luca F; Janson C; Sargur R; Dubois N; Kajosaari M; Wolters PJ; Chapman HA; Kampe O; Anderson MS. 2013. BPIFB1 is a lung-specific autoantigen associated with interstitial lung disease. Sci Transl Med 5(206):206ra139. [PubMed: 24107778]  [MGI Ref ID J:213455]

Shum AK; DeVoss J; Tan CL; Hou Y; Johannes K; O'Gorman CS; Jones KD; Sochett EB; Fong L; Anderson MS. 2009. Identification of an autoantigen demonstrates a link between interstitial lung disease and a defect in central tolerance. Sci Transl Med 1(9):9ra20. [PubMed: 20368189]  [MGI Ref ID J:167884]

Su MA; Giang K; Zumer K; Jiang H; Oven I; Rinn JL; Devoss JJ; Johannes KP; Lu W; Gardner J; Chang A; Bubulya P; Chang HY; Peterlin BM; Anderson MS. 2008. Mechanisms of an autoimmunity syndrome in mice caused by a dominant mutation in Aire. J Clin Invest 118(5):1712-26. [PubMed: 18414681]  [MGI Ref ID J:135154]

Taniguchi RT; Devoss JJ; Moon JJ; Sidney J; Sette A; Jenkins MK; Anderson MS. 2012. Detection of an autoreactive T-cell population within the polyclonal repertoire that undergoes distinct autoimmune regulator (Aire)-mediated selection. Proc Natl Acad Sci U S A 109(20):7847-52. [PubMed: 22552229]  [MGI Ref ID J:184783]

Venanzi ES; Gray DH; Benoist C; Mathis D. 2007. Lymphotoxin pathway and Aire influences on thymic medullary epithelial cells are unconnected. J Immunol 179(9):5693-700. [PubMed: 17947641]  [MGI Ref ID J:153012]

Venanzi ES; Melamed R; Mathis D; Benoist C. 2008. The variable immunological self: genetic variation and nongenetic noise in Aire-regulated transcription. Proc Natl Acad Sci U S A 105(41):15860-5. [PubMed: 18838677]  [MGI Ref ID J:141423]

Vijmasi T; Chen FY; Chen YT; Gallup M; McNamara N. 2013. Topical administration of interleukin-1 receptor antagonist as a therapy for aqueous-deficient dry eye in autoimmune disease. Mol Vis 19:1957-65. [PubMed: 24068863]  [MGI Ref ID J:205292]

Yang S; Bansal K; Lopes J; Benoist C; Mathis D. 2013. Aire's plant homeodomain(PHD)-2 is critical for induction of immunological tolerance. Proc Natl Acad Sci U S A 110(5):1833-8. [PubMed: 23319629]  [MGI Ref ID J:193695]

Zhang X; Liu JQ; Shi Y; Reid HH; Boyd RL; Khattabi M; El-Omrani HY; Zheng P; Liu Y; Bai XF. 2012. CD24 on thymic APCs regulates negative selection of myelin antigen-specific T lymphocytes. Eur J Immunol 42(4):924-35. [PubMed: 22213356]  [MGI Ref ID J:187788]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Approximately 79% of homozygotes die spontaneously or must be euthanized due to declining health at between 6 and 14 weeks of age.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

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Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   Wild-type from the colony
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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