| |||||||||||||||||||
| These Osx1-GFP::Cre mutant mice may be useful in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 14-APR-08 Species laboratory mouse Generation N5+N4F11 (27-DEC-12)
Generation DefinitionsDonating Investigator Andrew P McMahon, University of Southern California Description
Mice hemizygous for this Osx1-GFP::Cre transgene are viable and fertile. The transgene carries both tTA under the regulation of the osterix (Sp7) promoter and, just downstream, a tetracycline responsive element (TRE; tetO)-controlled GFP/Cre fusion protein. In the absence of the tetracycline analog doxycycline, EGFP-Cre fusion protein expression is restricted to the osteoblast lineage throughout embryonic and early postnatal development. Fusion protein activity is largely absent from chondrocytes. When these transgenic animals are mated to transgenic strains that carry loxP-flanked (floxed) conditional alleles, Cre-mediated recombination of the floxed allele in the double mutant animals is placed under the regulation of doxycycline (dox) such that dox adminstration prevents fusion protein expression and recombination. The donating investigator suggests that the mice be maintained on dox-treated water to avoid incidental effects of tTA expression (e.g., malocclusion). These Osx1-GFP::Cre mutant mice may be useful in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling.Development
A bacterial artificial chromosome (BAC; RP23-399N14) was modified by the insertion of "tTA-regulated GFP::Cre" construct into exon 1 of the mouse Sp7 gene (formerly called osterix or Osx1). This construct was designed with the tetracycline-regulated transactivator (tTA or "Tet-Off") cassette just upstream of a tetracycline-responsive element (TRE; tetO)-controlled Enhanced Greed Fluorescent Protein/Cre (EGFP/Cre) fusion protein, all followed by an frt-flanked kanamycin selection cassette. Correctly targeted bacteria were treated to remove the selection cassette (leaving a single frt site). The resulting modified BAC, called Osx1-GFP::Cre, was microinjected into fertilized CD-1 embryos. Trangenic mice were then mated to C57BL/6 mice for at least 5 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedDevelopmental Biology Research
Skeletal Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tet Expression Systems
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Sp7-tTA,tetO-EGFP/cre)1Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andrew P McMahon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Osx-Cre; Osx-Cre::GFP; Osx1-GFP::Cre; Osx1-cre; osterix-Cre; | ||
| Mutation Made By | Andrew McMahon, University of Southern California | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | Expresses tTA in osteoblasts; addition of tetracycline/doxycycline halts EGFG/cre fusion protein expression. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | tetO, tet operator, | ||
| Driver Note | Sp7 | ||
| Inducible Note | induced by doxycycline | ||
| Molecular Note | A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modifiy a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). A frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression.A 5.2 kb portionof the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos. [MGI Ref ID J:114494] | ||
| Gene Symbol and Name | Tg(Sp7-tTA,tetO-EGFP/cre)1Amc, transgene insertion 1, Andrew P McMahon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Osx1-GFP::Cre; osterix-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Rodda SJ; McMahon AP. 2006. Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors. Development 133(16):3231-44. [PubMed: 16854976] [MGI Ref ID J:114494]
Additional References
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc relatedAliprantis AO; Ueki Y; Sulyanto R; Park A; Sigrist KS; Sharma SM; Ostrowski MC; Olsen BR; Glimcher LH. 2008. NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopenia from inflammation in cherubism. J Clin Invest 118(11):3775-89. [PubMed: 18846253] [MGI Ref ID J:144598]
Almeida M; Iyer S; Martin-Millan M; Bartell SM; Han L; Ambrogini E; Onal M; Xiong J; Weinstein RS; Jilka RL; O'Brien CA; Manolagas SC. 2013. Estrogen receptor-alpha signaling in osteoblast progenitors stimulates cortical bone accrual. J Clin Invest 123(1):394-404. [PubMed: 23221342] [MGI Ref ID J:194156]
Berman SD; Calo E; Landman AS; Danielian PS; Miller ES; West JC; Fonhoue BD; Caron A; Bronson R; Bouxsein ML; Mukherjee S; Lees JA. 2008. Metastatic osteosarcoma induced by inactivation of Rb and p53 in the osteoblast lineage. Proc Natl Acad Sci U S A 105(33):11851-6. [PubMed: 18697945] [MGI Ref ID J:139697]
Calo E; Quintero-Estades JA; Danielian PS; Nedelcu S; Berman SD; Lees JA. 2010. Rb regulates fate choice and lineage commitment in vivo. Nature 466(7310):1110-4. [PubMed: 20686481] [MGI Ref ID J:163313]
Chang W; Tu C; Chen TH; Bikle D; Shoback D. 2008. The extracellular calcium-sensing receptor (CaSR) is a critical modulator of skeletal development. Sci Signal 1(35):ra1. [PubMed: 18765830] [MGI Ref ID J:180651]
Cook JR; Smaldone S; Cozzolino C; Del Solar M; Lee-Arteaga S; Nistala H; Ramirez F. 2012. Generation of Fbn1 conditional null mice implicates the extracellular microfibrils in osteoprogenitor recruitment. Genesis 50(8):635-41. [PubMed: 22374917] [MGI Ref ID J:187484]
Davey RA; Clarke MV; Sastra S; Skinner JP; Chiang C; Anderson PH; Zajac JD. 2012. Decreased body weight in young Osterix-Cre transgenic mice results in delayed cortical bone expansion and accrual. Transgenic Res 21(4):885-93. [PubMed: 22160436] [MGI Ref ID J:186593]
Greenbaum A; Hsu YM; Day RB; Schuettpelz LG; Christopher MJ; Borgerding JN; Nagasawa T; Link DC. 2013. CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance. Nature 495(7440):227-30. [PubMed: 23434756] [MGI Ref ID J:193594]
Greenbaum AM; Revollo LD; Woloszynek JR; Civitelli R; Link DC. 2012. N-cadherin in osteolineage cells is not required for maintenance of hematopoietic stem cells. Blood 120(2):295-302. [PubMed: 22323481] [MGI Ref ID J:188098]
Greenblatt MB; Shim JH; Zou W; Sitara D; Schweitzer M; Hu D; Lotinun S; Sano Y; Baron R; Park JM; Arthur S; Xie M; Schneider MD; Zhai B; Gygi S; Davis R; Glimcher LH. 2010. The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice. J Clin Invest 120(7):2457-73. [PubMed: 20551513] [MGI Ref ID J:163789]
Hou B; Kolpakova-Hart E; Fukai N; Wu K; Olsen BR. 2009. The polycystic kidney disease 1 (Pkd1) gene is required for the responses of osteochondroprogenitor cells to midpalatal suture expansion in mice. Bone 44(6):1121-33. [PubMed: 19264154] [MGI Ref ID J:150386]
Kashiwagi A; Fein MJ; Shimada M. 2011. A high fat diet-induced impaired glucose metabolism in mice with targeted deletion of calpain in osteoblasts. Biochem Biophys Res Commun 409(2):235-40. [PubMed: 21569760] [MGI Ref ID J:172595]
Kobayashi T; Lu J; Cobb BS; Rodda SJ; McMahon AP; Schipani E; Merkenschlager M; Kronenberg HM. 2008. Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proc Natl Acad Sci U S A 105(6):1949-54. [PubMed: 18238902] [MGI Ref ID J:131828]
Kolpakova-Hart E; McBratney-Owen B; Hou B; Fukai N; Nicolae C; Zhou J; Olsen BR. 2008. Growth of cranial synchondroses and sutures requires polycystin-1. Dev Biol 321(2):407-19. [PubMed: 18652813] [MGI Ref ID J:139970]
Lane SW; De Vita S; Alexander KA; Karaman R; Milsom MD; Dorrance AM; Purdon A; Louis L; Bouxsein ML; Williams DA. 2012. Rac signaling in osteoblastic cells is required for normal bone development but is dispensable for hematopoietic development. Blood 119(3):736-44. [PubMed: 22123845] [MGI Ref ID J:181778]
Lehoczky JA; Robert B; Tabin CJ. 2011. Mouse digit tip regeneration is mediated by fate-restricted progenitor cells. Proc Natl Acad Sci U S A 108(51):20609-14. [PubMed: 22143790] [MGI Ref ID J:180520]
Liu Y; Berendsen AD; Jia S; Lotinun S; Baron R; Ferrara N; Olsen BR. 2012. Intracellular VEGF regulates the balance between osteoblast and adipocyte differentiation. J Clin Invest 122(9):3101-13. [PubMed: 22886301] [MGI Ref ID J:191431]
Maeda K; Kobayashi Y; Udagawa N; Uehara S; Ishihara A; Mizoguchi T; Kikuchi Y; Takada I; Kato S; Kani S; Nishita M; Marumo K; Martin TJ; Minami Y; Takahashi N. 2012. Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis. Nat Med 18(3):405-12. [PubMed: 22344299] [MGI Ref ID J:181641]
Maes C; Goossens S; Bartunkova S; Drogat B; Coenegrachts L; Stockmans I; Moermans K; Nyabi O; Haigh K; Naessens M; Haenebalcke L; Tuckermann JP; Tjwa M; Carmeliet P; Mandic V; David JP; Behrens A; Nagy A; Carmeliet G; Haigh JJ. 2010. Increased skeletal VEGF enhances beta-catenin activity and results in excessively ossified bones. EMBO J 29(2):424-41. [PubMed: 20010698] [MGI Ref ID J:156474]
Maes C; Kobayashi T; Selig MK; Torrekens S; Roth SI; Mackem S; Carmeliet G; Kronenberg HM. 2010. Osteoblast precursors, but not mature osteoblasts, move into developing and fractured bones along with invading blood vessels. Dev Cell 19(2):329-44. [PubMed: 20708594] [MGI Ref ID J:163667]
Moisan A; Rivera MN; Lotinun S; Akhavanfard S; Coffman EJ; Cook EB; Stoykova S; Mukherjee S; Schoonmaker JA; Burger A; Kim WJ; Kronenberg HM; Baron R; Haber DA; Bardeesy N. 2011. The WTX tumor suppressor regulates mesenchymal progenitor cell fate specification. Dev Cell 20(5):583-96. [PubMed: 21571217] [MGI Ref ID J:173242]
Nistala H; Lee-Arteaga S; Smaldone S; Siciliano G; Carta L; Ono RN; Sengle G; Arteaga-Solis E; Levasseur R; Ducy P; Sakai LY; Karsenty G; Ramirez F. 2010. Fibrillin-1 and -2 differentially modulate endogenous TGF-beta and BMP bioavailability during bone formation. J Cell Biol 190(6):1107-21. [PubMed: 20855508] [MGI Ref ID J:165256]
Raaijmakers MH; Mukherjee S; Guo S; Zhang S; Kobayashi T; Schoonmaker JA; Ebert BL; Al-Shahrour F; Hasserjian RP; Scadden EO; Aung Z; Matza M; Merkenschlager M; Lin C; Rommens JM; Scadden DT. 2010. Bone progenitor dysfunction induces myelodysplasia and secondary leukaemia. Nature 464(7290):852-7. [PubMed: 20305640] [MGI Ref ID J:158901]
Rankin EB; Wu C; Khatri R; Wilson TL; Andersen R; Araldi E; Rankin AL; Yuan J; Kuo CJ; Schipani E; Giaccia AJ. 2012. The HIF signaling pathway in osteoblasts directly modulates erythropoiesis through the production of EPO. Cell 149(1):63-74. [PubMed: 22464323] [MGI Ref ID J:186085]
Razidlo DF; Whitney TJ; Casper ME; McGee-Lawrence ME; Stensgard BA; Li X; Secreto FJ; Knutson SK; Hiebert SW; Westendorf JJ. 2010. Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat. PLoS One 5(7):e11492. [PubMed: 20628553] [MGI Ref ID J:163120]
Regard JB; Cherman N; Palmer D; Kuznetsov SA; Celi FS; Guettier JM; Chen M; Bhattacharyya N; Wess J; Coughlin SR; Weinstein LS; Collins MT; Robey PG; Yang Y. 2011. Wnt/beta-catenin signaling is differentially regulated by Galpha proteins and contributes to fibrous dysplasia. Proc Natl Acad Sci U S A 108(50):20101-6. [PubMed: 22106277] [MGI Ref ID J:180451]
Schmidt E; Nilsson O; Koskela A; Tuukkanen J; Ohlsson C; Rozell B; Eriksson M. 2012. Expression of the Hutchinson-Gilford progeria mutation during osteoblast development results in loss of osteocytes, irregular mineralization, and poor biomechanical properties. J Biol Chem 287(40):33512-22. [PubMed: 22893709] [MGI Ref ID J:191583]
Shim JH; Greenblatt MB; Singh A; Brady N; Hu D; Drapp R; Ogawa W; Kasuga M; Noda T; Yang SH; Lee SK; Rebel VI; Glimcher LH. 2012. Administration of BMP2/7 in utero partially reverses Rubinstein-Taybi syndrome-like skeletal defects induced by Pdk1 or Cbp mutations in mice. J Clin Invest 122(1):91-106. [PubMed: 22133875] [MGI Ref ID J:184575]
Shimada M; Greer PA; McMahon AP; Bouxsein ML; Schipani E. 2008. In vivo targeted deletion of calpain small subunit, Capn4, in cells of the osteoblast lineage impairs cell proliferation, differentiation, and bone formation. J Biol Chem 283(30):21002-10. [PubMed: 18515801] [MGI Ref ID J:137992]
Shiozawa Y; Taichman RS. 2010. Dysfunctional niches as a root of hematopoietic malignancy. Cell Stem Cell 6(5):399-400. [PubMed: 20452309] [MGI Ref ID J:160606]
Tu X; Chen J; Lim J; Karner CM; Lee SY; Heisig J; Wiese C; Surendran K; Kopan R; Gessler M; Long F. 2012. Physiological notch signaling maintains bone homeostasis via RBPjk and Hey upstream of NFATc1. PLoS Genet 8(3):e1002577. [PubMed: 22457635] [MGI Ref ID J:183534]
Walkley CR; Qudsi R; Sankaran VG; Perry JA; Gostissa M; Roth SI; Rodda SJ; Snay E; Dunning P; Fahey FH; Alt FW; McMahon AP; Orkin SH. 2008. Conditional mouse osteosarcoma, dependent on p53 loss and potentiated by loss of Rb, mimics the human disease. Genes Dev 22(12):1662-76. [PubMed: 18559481] [MGI Ref ID J:136693]
Wu JY; Aarnisalo P; Bastepe M; Sinha P; Fulzele K; Selig MK; Chen M; Poulton IJ; Purton LE; Sims NA; Weinstein LS; Kronenberg HM. 2011. Gsalpha enhances commitment of mesenchymal progenitors to the osteoblast lineage but restrains osteoblast differentiation in mice. J Clin Invest 121(9):3492-504. [PubMed: 21804192] [MGI Ref ID J:178262]
Wu JY; Purton LE; Rodda SJ; Chen M; Weinstein LS; McMahon AP; Scadden DT; Kronenberg HM. 2008. Osteoblastic regulation of B lymphopoiesis is mediated by Gs{alpha}-dependent signaling pathways. Proc Natl Acad Sci U S A 105(44):16976-81. [PubMed: 18957542] [MGI Ref ID J:144059]
Xiong J; Onal M; Jilka RL; Weinstein RS; Manolagas SC; O'Brien CA. 2011. Matrix-embedded cells control osteoclast formation. Nat Med 17(10):1235-41. [PubMed: 21909103] [MGI Ref ID J:177558]
Zhu W; Liang G; Huang Z; Doty SB; Boskey AL. 2011. Conditional inactivation of the CXCR4 receptor in osteoprecursors reduces postnatal bone formation due to impaired osteoblast development. J Biol Chem 286(30):26794-805. [PubMed: 21636574] [MGI Ref ID J:175383]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings or C57BL/6J. Alternatively, transgenic mice may be bred together, although a homozygous phenotype is not yet characterized (Sep-2006). The donating investigator suggests that mice be maintained on doxycycline-treated water to avoid incidental effects of tTA expression. Mating System Noncarrier x Hemizygote (Female x Male) 14-APR-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.