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| These Osx1-GFP::Cre mutant mice may be useful in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling. | |||||||||||||||
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- Genes & alleles
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 14-APR-08 Species laboratory mouse Generation N5+N4F3 (13-JAN-09) Donating Investigator Andrew McMahon, Harvard University Description
Mice hemizygous for this Osx1-GFP::Cre transgene are viable and fertile. The transgene carries both tTA under the regulation of the osterix (Sp7) promoter and, just downstream, a tetracycline responsive element (TRE; tetO)-controlled GFP/Cre fusion protein. In the absence of the tetracycline analog doxycycline, EGFP-Cre fusion protein expression is restricted to the osteoblast lineage throughout embryonic and early postnatal development. Fusion protein activity is largely absent from chondrocytes. When these transgenic animals are mated to transgenic strains that carry loxP-flanked (floxed) conditional alleles, Cre-mediated recombination of the floxed allele in the double mutant animals is placed under the regulation of doxycycline (dox) such that dox adminstration prevents fusion protein expression and recombination. The donating investigator suggests that the mice be maintained on dox-treated water to avoid incidental effects of tTA expression. These Osx1-GFP::Cre mutant mice may be useful in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling.Development
A bacterial artificial chromosome (BAC; RP23-399N14) was modified by the insertion of "tTA-regulated GFP::Cre" construct into exon 1 of the mouse Sp7 gene (formerly called osterix or Osx1). This construct was designed with the tetracycline-regulated transactivator (tTA or "Tet-Off") cassette just upstream of a tetracycline-responsive element (TRE; tetO)-controlled Enhanced Greed Fluorescent Protein/Cre (EGFP/Cre) fusion protein, all followed by an frt-flanked kanamycin selection cassette. Correctly targeted bacteria were treated to remove the selection cassette (leaving a single frt site). The resulting modified BAC, called Osx1-GFP::Cre, was microinjected into fertilized CD-1 embryos. Trangenic mice were then mated to C57BL/6 mice for at least 5 generations prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 008284 B6.Cg-Tg(Scg2-tTA)1Jt/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 010573 B6;SJL-Tg(Prl-tTA)6-5Jek/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 009606 STOCK Tg(Six2-EGFP/cre)1Amc/J 003271 STOCK Tg(tTAhCMV)3Bjd/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedDevelopmental Biology Research
Skeletal Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Tet Expression Systems
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Sp7-tTA,tetO-EGFP/cre)1Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andrew P McMahon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Osx1-GFP::Cre; osterix-Cre; | ||
| Mutation Made By | Andrew McMahon, Harvard University | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | Expresses tTA in osteoblasts; addition of tetracycline/doxycycline halts EGFG/cre fusion protein expression. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | tetO, tet operator, | ||
| Promoter | Sp7, Sp7 transcription factor 7, mouse, laboratory | ||
| Driver Note | tetO | ||
| Inducible Note | induced by doxycycline | ||
| Molecular Note | A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modifiy a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). A frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression.A 5.2 kb portionof the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos. [MGI Ref ID J:114494] | ||
| Gene Symbol and Name | Tg(Sp7-tTA,tetO-EGFP/cre)1Amc, transgene insertion 1, Andrew P McMahon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Osx1-GFP::Cre; osterix-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Rodda SJ; McMahon AP. 2006. Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors. Development 133(16):3231-44. [PubMed: 16854976] [MGI Ref ID J:114494]
Additional References
Tg(Sp7-tTA,tetO-EGFP/cre)1Amc relatedAliprantis AO; Ueki Y; Sulyanto R; Park A; Sigrist KS; Sharma SM; Ostrowski MC; Olsen BR; Glimcher LH. 2008. NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopenia from inflammation in cherubism. J Clin Invest 118(11):3775-89. [PubMed: 18846253] [MGI Ref ID J:144598]
Berman SD; Calo E; Landman AS; Danielian PS; Miller ES; West JC; Fonhoue BD; Caron A; Bronson R; Bouxsein ML; Mukherjee S; Lees JA. 2008. Metastatic osteosarcoma induced by inactivation of Rb and p53 in the osteoblast lineage. Proc Natl Acad Sci U S A 105(33):11851-6. [PubMed: 18697945] [MGI Ref ID J:139697]
Hou B; Kolpakova-Hart E; Fukai N; Wu K; Olsen BR. 2009. The polycystic kidney disease 1 (Pkd1) gene is required for the responses of osteochondroprogenitor cells to midpalatal suture expansion in mice. Bone 44(6):1121-33. [PubMed: 19264154] [MGI Ref ID J:150386]
Kobayashi T; Lu J; Cobb BS; Rodda SJ; McMahon AP; Schipani E; Merkenschlager M; Kronenberg HM. 2008. Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proc Natl Acad Sci U S A 105(6):1949-54. [PubMed: 18238902] [MGI Ref ID J:131828]
Kolpakova-Hart E; McBratney-Owen B; Hou B; Fukai N; Nicolae C; Zhou J; Olsen BR. 2008. Growth of cranial synchondroses and sutures requires polycystin-1. Dev Biol 321(2):407-19. [PubMed: 18652813] [MGI Ref ID J:139970]
Shimada M; Greer PA; McMahon AP; Bouxsein ML; Schipani E. 2008. In vivo targeted deletion of calpain small subunit, Capn4, in cells of the osteoblast lineage impairs cell proliferation, differentiation, and bone formation. J Biol Chem 283(30):21002-10. [PubMed: 18515801] [MGI Ref ID J:137992]
Walkley CR; Qudsi R; Sankaran VG; Perry JA; Gostissa M; Roth SI; Rodda SJ; Snay E; Dunning P; Fahey FH; Alt FW; McMahon AP; Orkin SH. 2008. Conditional mouse osteosarcoma, dependent on p53 loss and potentiated by loss of Rb, mimics the human disease. Genes Dev 22(12):1662-76. [PubMed: 18559481] [MGI Ref ID J:136693]
Wu JY; Purton LE; Rodda SJ; Chen M; Weinstein LS; McMahon AP; Scadden DT; Kronenberg HM. 2008. Osteoblastic regulation of B lymphopoiesis is mediated by Gs{alpha}-dependent signaling pathways. Proc Natl Acad Sci U S A 105(44):16976-81. [PubMed: 18957542] [MGI Ref ID J:144059]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice can be bred to wildtype siblings or C57BL/6J. Alternatively, transgenic mice may be bred together, although a homozygous phenotype is not yet characterized (Sep-2006). The donating investigator suggests that mice be maintained on doxycycline-treated water to avoid incidental effects of tTA expression. Mating System Noncarrier x Hemizygote (Female x Male) 14-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
Pairs /Price (US dollars $) Pair Genotype $297.85 Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc x Noncarrier $297.85 Noncarrier x Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
Pairs /Price (US dollars $) Pair Genotype $387.30 Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc x Noncarrier $387.30 Noncarrier x Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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