Strain Name: |
B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J |
|---|---|
Stock Number: |
006361 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | GFP; Sp7; cre; tTA; Tg(Sp7-tTA,tetO-EGFP/cre)1Amc; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Noncarrier x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Andrew McMahon, Harvard University Generation N5+N4F1 (23-APR-08) Strain Description
Mice hemizygous for this Osx1-GFP::Cre transgene are viable and fertile. The transgene carries both tTA under the regulation of the osterix (Sp7) promoter and, just downstream, a tetracycline responsive element (TRE; tetO)-controlled GFP/Cre fusion protein. In the absence of the tetracycline analog doxycycline, EGFP-Cre fusion protein expression is restricted to the osteoblast lineage throughout embryonic and early postnatal development. Fusion protein activity is largely absent from chondrocytes. When these transgenic animals are mated to transgenic strains that carry loxP-flanked (floxed) conditional alleles, Cre-mediated recombination of the floxed allele in the double mutant animals is placed under the regulation of doxycycline (dox) such that dox adminstration prevents fusion protein expression and recombination. The donating investigator suggests that the mice be maintained on dox-treated water to avoid incidental effects of tTA expression. These Osx1-GFP::Cre mutant mice may be useful in studies of bone development, osteoblast lineage, and Hedhehog/Wnt signaling.Strain Development
A bacterial artificial chromosome (BAC; RP23-399N14) was modified by the insertion of "tTA-regulated GFP::Cre" construct into exon 1 of the mouse Sp7 gene (formerly called osterix or Osx1). This construct was designed with the tetracycline-regulated transactivator (tTA or "Tet-Off") cassette just upstream of a tetracycline-responsive element (TRE; tetO)-controlled Enhanced Greed Fluorescent Protein/Cre (EGFP/Cre) fusion protein, all followed by an frt-flanked kanamycin selection cassette. Correctly targeted bacteria were treated to remove the selection cassette (leaving a single frt site). The resulting modified BAC, called Osx1-GFP::Cre, was microinjected into fertilized CD-1 embryos. Trangenic mice were then mated to C57BL/6 mice for at least 5 generations prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Sp7-tTA,tetO-EGFP/cre)1Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Andrew P McMahon | ||
| Common Name(s) | Osx1-GFP::Cre; | ||
| Mutation Made By | Andrew McMahon, Harvard University | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | Expresses tTA in osteoblasts; addition of tetracycline/doxycycline halts EGFG/cre fusion protein expression. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | Sp7, trans-acting transcription factor 7, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A tTA-regulated GFP::Cre construct, which has the tetracycline-regulated transactivator (tTA) cassette upstream of a tetracycline-responsive element (tetO)-controlled Enhanced Green Fluorescent Protein/cre fusion protein was used to modifiy a bacterial artificial chromosome (BAC) encoding exon 1 of the mouse Sp7 gene (formerly osterix or Osx1). A frt-flanked selection cassette following the tTA-regulated GFP::Cre was removed from correctly targeted bacteria via transient flpe expression.A 5.2 kb portionof the modified BAC (Osx1-GFP::Cre) was used for pronuclear injection into fertilized CD1 embryos. [MGI Ref ID J:114494] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice can be bred to wildtype siblings or C57BL/6J. Alternatively, transgenic mice may be bred together, although a homozygous phenotype is not yet characterized (Sep-2006). The donating investigator suggests that mice be maintained on doxycycline-treated water to avoid incidental effects of tTA expression. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (139 strains)
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 003272 STOCK Tg(tTALap)5Bjd/J 003271 STOCK Tg(tTAhCMV)3Bjd/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedDevelopmental Biology Research
Skeletal Defects
Research Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Developmental Biology Research (Cre-lox System)
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Tet Expression Systems (tTA/rtTA Expressing Strains)
Tet Expression Systems (tTA/rtTA Responsive Strains)
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox SystemtTA related
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Tet Expression Systems
References
Selected Reference(s)
Additional ReferencesRodda SJ; McMahon AP. 2006. Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors. Development 133(16):3231-44. [PubMed: 16854976] [MGI Ref ID J:114494]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J |
| Stock Number: | 006361 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc | |||
| Pair | $375.30 | Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Sp7-tTA,tetO-EGFP/cre)1Amc | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
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