Strain Name: |
FVB-Tg(Nr5a1-cre)2Lowl/J |
|---|---|
Stock Number: |
006364 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Nr5a1; cre; Tg(Nr5a1-cre)2Lowl; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Bradford Lowell, Beth Israel Deaconess Medical Center Generation N?+N2 (10-JAN-08) Strain Description
Mice hemizygous for the "Sf1-Cre" transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression mimics the mRNA pattern of Nr5a1; with Cre activity observed in steroidogenic factor-1 (SF1)-positive neurons in the ventromedial hypothalamic nucleus (VMH) as well as pituitary, gonad, and adrenal tissue. Expression is also noted in the cerebral cortex and in a few scattered cells in the caudal brainstem of mice derived from line 2 (but not line 7). If bred with mice containing a loxP-flanked sequence of interest, tissue-specific deletion of that genome results in the offspring. Specifically, these cre-expressing mice may be useful in studies involving the hypothalamus, such as body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for SF1-transcription factor activity.
Gene & Allele Details
| Allele Symbol | Tg(Nr5a1-cre)2Lowl | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Bradford B Lowell | ||
| Common Name(s) | Sf1-cre; | ||
| Mutation Made By | Bradford Lowell, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | FVB | ||
| Site of Expression | in ventromedial hypothalamic nucleus, cerebral cortex, and a few scattered cells in the caudal brainstem, as well as in pituitary, gonad, and adrenal tissue | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Nr5a1, nuclear receptor subfamily 5, group A, member 1, mouse, laboratory | ||
| Molecular Note | This transgene contains cre recombinase under the control of Nr5a1 regulatory sequence. [MGI Ref ID J:105327] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Nr5a1-cre)2Lowl
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice can be bred to wildtype siblings or FVB/NJ. Alternatively, transgenic mice may be bred together, although the phenotype of homozygous mice is not known. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Nr5a1
007041 B6Ei.129P2-Nr5a1tm2Klp/EiJ 003219 D2.129P2(B6)-Nr5a1tm1Klp/EiJ 007042 D2.129P2(B6)-Nr5a1tm2Klp/EiJ View Strains carrying other alleles of Nr5a1 (3 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedEndocrine Deficiency Research
Hypothalamus/Pituitary Defects
Neurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Diabetes and Obesity Research
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesDhillon H; Zigman JM; Ye C; Lee CE; McGovern RA; Tang V; Kenny CD; Christiansen LM; White RD; Edelstein EA; Coppari R; Balthasar N; Cowley MA; Chua S Jr; Elmquist JK; Lowell BB. 2006. Leptin directly activates SF1 neurons in the VMH, and this action by leptin is required for normal body-weight homeostasis. Neuron 49(2):191-203. [PubMed: 16423694] [MGI Ref ID J:105327]
Price and Supply Information
| Strain Name: | FVB-Tg(Nr5a1-cre)2Lowl/J |
| Stock Number: | 006364 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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