Strain Name:

B6.Cg-Dicer1tm1Bdh/J

Stock Number:

006366

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Availability:

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Use Restrictions Apply, see Terms of Use
These mice contain loxP sites on either side of exon 23 of the Dicer1 gene. Cre-mediated recombination in the germline leads to developmental arrest. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   28-JAN-09
Specieslaboratory mouse
GenerationN10F11 (13-JAN-14)
Generation Definitions
 
Donating Investigator Brian D Harfe,   University of Florida

Description
These mice contain loxP sites on either side of exon 23. Homozygous mice are viable and fertile with no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wild-type despite the frt-flanked neomycin cassette. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion has been shown to result in loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.

For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb morphogenesis.

When bred to a strain expressing Cre recombinase in the neural tube (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of DiGeorge syndrome, miRNA biogenesis and neural crest cell development.

When bred to a strain expressing Cre recombinase in the distal posterior region of E10-E12 embryos (see Stock No. 005622 for example), this mutant mouse strain may be useful in studies of lung epithelial morphogenesis.

When bred to a strain expressing Cre recombinase in Foxp3+ cells from the lymph nodes, spleen and thymus (see Stock No. 23161 for example), this mutant mouse strain may be useful in studies of miRNA and the function of T reg cells.

When bred to a strain expressing Cre recombinase in Foxp3+ regulatory T cells (Foxp3tm4(YFP/cre)Ayr; see Stock No. 016959 for example), this mutant mouse strain may be useful in studies inflammation and the function of T reg cells.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector was used to flank exon 23 (encoding most of the second RNaseIII domain) of the endogenous gene with loxP sites. The vector also contained an frt-flanked neomycin resistance cassette between the exon and downstream loxP site. This construct was electroporated into "129" embryonic stem (ES) cells. Correctly targeted cells were injected into C57BL/6J blastocysts and chimeric mice were bred to C57BL/6J for germline transmission. Heterozygous offspring were bred to generate homozygotes. At some point, homozygotes were bred to mutant Gt(ROSA)26Sor mice on an unknown background. Upon arrival at The Jackson Laboratory, mice on the mixed genetic background were bred to select for the "Dicer-flox" allele and against the Gt(ROSA)26Sor reporter allele, resulting in Stock No. 006001. Mice from that colony were subsequently backcrossed to C57BL/6J for at least five generations to create these mice (Stock No. 006366).

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Dicer1tm1Bdh allele
006001   STOCK Dicer1tm1Bdh/J
View Strains carrying   Dicer1tm1Bdh     (1 strain)

Strains carrying other alleles of Dicer1
014167   129S-Dicer1tm1.1Mnn/J
012284   B6;129S7-Dicer1tm1Smr/J
View Strains carrying other alleles of Dicer1     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
DiGeorge Syndrome; DGS
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Goiter, Multinodular 1, with or without Sertoli-Leydig Cell Tumors;   (DICER1)
Pleuropulmonary Blastoma; PPB   (DICER1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Dicer1tm1Bdh/Dicer1+ Tg(Wnt1-cre)11Rth/0

        involves: 129 * C57BL/6 * CBA/J   (conditional)
  • hematopoietic system phenotype
  • abnormal thymus development
    • thymus development is delayed at E14.5, but by E16.5, the thymus is indistinguishable from wild-type   (MGI Ref ID J:166758)
  • immune system phenotype
  • abnormal thymus development
    • thymus development is delayed at E14.5, but by E16.5, the thymus is indistinguishable from wild-type   (MGI Ref ID J:166758)
  • endocrine/exocrine gland phenotype
  • abnormal thymus development
    • thymus development is delayed at E14.5, but by E16.5, the thymus is indistinguishable from wild-type   (MGI Ref ID J:166758)

Dicer1tm1Bdh/Dicer1tm1Bdh Foxp3tm4(YFP/cre)Ayr/Foxp3tm4(YFP/cre)Ayr

        involves: 129S1/Sv * 129X1/SvJ   (conditional)
  • mortality/aging
  • premature death
    • males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days   (MGI Ref ID J:138683)
  • immune system phenotype
  • enlarged lymph nodes   (MGI Ref ID J:138683)
  • enlarged spleen   (MGI Ref ID J:138683)
  • liver inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)
  • lung inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)
  • vascular inflammation
    • cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed   (MGI Ref ID J:138683)
  • hematopoietic system phenotype
  • enlarged spleen   (MGI Ref ID J:138683)
  • cardiovascular system phenotype
  • vascular inflammation
    • cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed   (MGI Ref ID J:138683)
  • liver/biliary system phenotype
  • liver inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)
  • respiratory system phenotype
  • lung inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)

Dicer1tm1Bdh/Dicer1tm1Bdh Foxp3tm4(YFP/cre)Ayr/Y

        involves: 129S1/Sv * 129X1/SvJ   (conditional)
  • mortality/aging
  • premature death
    • females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days   (MGI Ref ID J:138683)
  • immune system phenotype
  • enlarged lymph nodes   (MGI Ref ID J:138683)
  • enlarged spleen   (MGI Ref ID J:138683)
  • liver inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)
  • lung inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)
  • vascular inflammation
    • cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed   (MGI Ref ID J:138683)
  • hematopoietic system phenotype
  • enlarged spleen   (MGI Ref ID J:138683)
  • cardiovascular system phenotype
  • vascular inflammation
    • cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed   (MGI Ref ID J:138683)
  • liver/biliary system phenotype
  • liver inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)
  • respiratory system phenotype
  • lung inflammation
    • large areas are infiltrated by inflammatory cells in moribund mice   (MGI Ref ID J:138683)

Dicer1tm1Bdh/Dicer1tm1Bdh Tg(Foxp3-EGFP/cre)1aJbs/0

        involves: 129 * NOD/ShiLt   (conditional)
  • mortality/aging
  • premature death
    • all mice die by 6-8 weeks of age   (MGI Ref ID J:138808)
  • growth/size/body phenotype
  • weight loss
    • aging animals display weight loss   (MGI Ref ID J:138808)
  • behavior/neurological phenotype
  • abnormal behavior
    • aging animals display reduced mobility   (MGI Ref ID J:138808)
  • immune system phenotype
  • abnormal CD4-positive T cell physiology
    • many effector T cells have high levels of Il4, Il10 and interferon gamma, Ifng whereas <1% of cells from wild-type express Il4, Il10, Il17 or Ifng   (MGI Ref ID J:138808)
  • abnormal effector T cell morphology
    • number of CTLA4-expressing CD4+ Foxp3- effector T cells increase to >50% of splenic and >70% of lymph node CD4+ T cells compared to <10% in wild-type littermates   (MGI Ref ID J:138808)
  • abnormal lymph node cell ratio
    • dramatic increase in lymph node T cells is seen in 4-6 week old mice; however percentage of regulatory T cells is maintained   (MGI Ref ID J:138808)
  • abnormal regulatory T cell morphology
    • T reg cells have a higher percentage of CD103+ T cells and greater number of CD62lo cells   (MGI Ref ID J:138808)
    • decreased regulatory T cell number
      • percentage of regulatory T (T reg) cells in spleen and peripheral blood is reduced to 33% of controls   (MGI Ref ID J:138808)
  • abnormal regulatory T cell physiology
    • CD4+ Foxp3+ T regs have a more prominent activated phenotype (increased expression of CD25, CTLA4 and glucocorticoid-induced TNFR)   (MGI Ref ID J:138808)
  • abnormal splenic cell ratio
    • dramatic increase in splenic T cells is seen in 4-6 week old mice relative to controls   (MGI Ref ID J:138808)
  • enlarged lymph nodes
    • observed in all aging animals (>5 weeks of age)   (MGI Ref ID J:138808)
  • enlarged spleen
    • observed in all aging animals (>5 weeks of age)   (MGI Ref ID J:138808)
  • liver inflammation
    • tissue displays extensive mononuclear cell infiltration   (MGI Ref ID J:138808)
  • lung inflammation
    • tissue displays extensive mononuclear cell infiltration   (MGI Ref ID J:138808)
    • tissue has dense predominantly peribronchovascular infiltrates, composed of CD4+ and Cd8+ T cells   (MGI Ref ID J:138808)
  • hematopoietic system phenotype
  • abnormal CD4-positive T cell physiology
    • many effector T cells have high levels of Il4, Il10 and interferon gamma, Ifng whereas <1% of cells from wild-type express Il4, Il10, Il17 or Ifng   (MGI Ref ID J:138808)
  • abnormal effector T cell morphology
    • number of CTLA4-expressing CD4+ Foxp3- effector T cells increase to >50% of splenic and >70% of lymph node CD4+ T cells compared to <10% in wild-type littermates   (MGI Ref ID J:138808)
  • abnormal regulatory T cell morphology
    • T reg cells have a higher percentage of CD103+ T cells and greater number of CD62lo cells   (MGI Ref ID J:138808)
    • decreased regulatory T cell number
      • percentage of regulatory T (T reg) cells in spleen and peripheral blood is reduced to 33% of controls   (MGI Ref ID J:138808)
  • abnormal regulatory T cell physiology
    • CD4+ Foxp3+ T regs have a more prominent activated phenotype (increased expression of CD25, CTLA4 and glucocorticoid-induced TNFR)   (MGI Ref ID J:138808)
  • abnormal splenic cell ratio
    • dramatic increase in splenic T cells is seen in 4-6 week old mice relative to controls   (MGI Ref ID J:138808)
  • enlarged spleen
    • observed in all aging animals (>5 weeks of age)   (MGI Ref ID J:138808)
  • liver/biliary system phenotype
  • liver inflammation
    • tissue displays extensive mononuclear cell infiltration   (MGI Ref ID J:138808)
  • respiratory system phenotype
  • lung inflammation
    • tissue displays extensive mononuclear cell infiltration   (MGI Ref ID J:138808)
    • tissue has dense predominantly peribronchovascular infiltrates, composed of CD4+ and Cd8+ T cells   (MGI Ref ID J:138808)
  • skeleton phenotype
  • kyphosis
    • seen in aging animals   (MGI Ref ID J:138808)

Dicer1tm1Bdh/Dicer1tm1Bdh Tg(Prrx1-cre)1Cjt/0

        involves: 129 * C57BL/6J * SJL/J   (conditional)
  • limbs/digits/tail phenotype
  • abnormal forelimb morphology
    • at E11 the forelimbs are smaller and at E12.5 the forelimb size resembles that of wild-type forelimbs at E11.5   (MGI Ref ID J:100475)
    • starting at E10.5 increased cell death is seen in the limb mesoderm   (MGI Ref ID J:100475)
  • abnormal hindlimb morphology
    • the hindlimbs are smaller but not as severely affected as the forelimbs   (MGI Ref ID J:100475)
    • starting at E12.5 increased cell death is seen in the limb mesoderm   (MGI Ref ID J:100475)
  • oligodactyly
    • forelimbs show loss of digits and some fusion of digits   (MGI Ref ID J:100475)
  • skeleton phenotype
  • abnormal long bone morphology
    • the long bones of the arms and legs appear twisted   (MGI Ref ID J:100475)
  • delayed endochondral bone ossification
    • bone formation from cartilage is delayed in the long bones of the limbs   (MGI Ref ID J:100475)

Dicer1tm1Bdh/Dicer1tm1Bdh Tg(Wnt1-cre)11Rth/0

        involves: 129 * C57BL/6 * CBA/J   (conditional)
  • mortality/aging
  • complete lethality throughout fetal growth and development
    • die soon after E16.5   (MGI Ref ID J:166758)
  • cardiovascular system phenotype
  • abnormal aortic arch development
    • discontinuance of the ascending aortic arch with the descending aorta, indicating improper pattering of the aortic arch due to a left fourth aortic arch defect   (MGI Ref ID J:166758)
  • persistent truncus arteriosis
    • the outflow tract does not fully septate into a pulmonary artery and aorta in some cases, resulting in the persistence of a common outflow vessel   (MGI Ref ID J:166758)
  • ventricular septal defect   (MGI Ref ID J:166758)
  • craniofacial phenotype
  • abnormal craniofacial morphology
    • severe craniofacial defects are seen by E14.5   (MGI Ref ID J:166758)
    • abnormal first pharyngeal arch morphology
      • although pharyngeal arches appear similar to wild-type in emergence, size and shape, expression of Dlx2 and Fgf8 are downregulated in pharyngeal arch 1   (MGI Ref ID J:166758)
      • apoptosis is increased in the first pharyngeal arch at E11.5   (MGI Ref ID J:166758)
  • embryogenesis phenotype
  • abnormal first pharyngeal arch morphology
    • although pharyngeal arches appear similar to wild-type in emergence, size and shape, expression of Dlx2 and Fgf8 are downregulated in pharyngeal arch 1   (MGI Ref ID J:166758)
    • apoptosis is increased in the first pharyngeal arch at E11.5   (MGI Ref ID J:166758)
  • hematopoietic system phenotype
  • athymia
    • thymus development is absent   (MGI Ref ID J:166758)
  • immune system phenotype
  • athymia
    • thymus development is absent   (MGI Ref ID J:166758)
  • nervous system phenotype
  • abnormal dorsal root ganglion morphology
    • loss of neural crest cell derived neuronal tissue from the dorsal root ganglia   (MGI Ref ID J:166758)
  • abnormal sympathetic ganglion morphology
    • loss of neural crest cell derived neuronal tissue from the thoracic sympathetic ganglia   (MGI Ref ID J:166758)
  • skeleton phenotype
  • abnormal cartilage morphology
    • neural crest cell derived maxillary and mandibular regions of the face and frontonasal process lack cartilaginous tissue, however mesodermally derived cartilage near the base of the skull is present   (MGI Ref ID J:166758)
  • endocrine/exocrine gland phenotype
  • athymia
    • thymus development is absent   (MGI Ref ID J:166758)

Dicer1tm1Bdh/Dicer1tm1Bdh Shhtm1(EGFP/cre)Cjt/Shh+

        involves: 129   (conditional)
  • mortality/aging
  • complete perinatal lethality
    • mice die at birth   (MGI Ref ID J:106072)
  • respiratory system phenotype
  • abnormal lung development
    • at E12.5, fewer branches are observed and the distal tips of the newly formed branches appear dilated   (MGI Ref ID J:106072)
    • the number of dilated branching tips observed at E12.5 corresponds to the number of large, fluid-filled sacs seen at E15.5   (MGI Ref ID J:106072)
    • defective branching morphogenesis occurs prior to the increased cell death seen in the distal epithelium at E13.0   (MGI Ref ID J:106072)
    • at E11.75, expression of Fgf10 (a key gene involved in lung development) is already up-regulated and expanded in the mesenchyme of mutant lungs   (MGI Ref ID J:106072)
  • abnormal lung epithelium morphology
    • at E15.5, mutant lung epithelial cells form large, fluid-filled sacs within each lobe, indicating a severe branching defect   (MGI Ref ID J:106072)
    • at E15.5, the mutant epithelium is often detached from the mesenchyme, unlike in control lungs   (MGI Ref ID J:106072)
    • at E12.25, increased epithelial cell death is ectopically observed in the secondary bronchi of mutant lungs in addition to the normal pattern of cell death seen in the trachea and primary bronchi   (MGI Ref ID J:106072)
    • at E12.5 and E12.75, intense epithelial cell death is prolonged in the mutant trachea and bronchi, unlike in control and wild type lungs   (MGI Ref ID J:106072)
    • by E13.0, aberrant cell death is observed in the entire mutant lung epithelium, including the distal branching region, but not in the mesenchyme   (MGI Ref ID J:106072)
  • small lung lobe
    • after E13.5, each mutant lung lobe is proportionally smaller than that in control lungs   (MGI Ref ID J:106072)
    • however, each lobe maintains a normal shape, indicating a normal lobation pattern   (MGI Ref ID J:106072)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cell Biology Research
Protein Processing
Transcriptional Regulation

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System
      Mutagenesis and Transgenesis: transcriptional activation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Dicer1tm1Bdh
Allele Name targeted mutation 1, Brian D Harfe
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Dcrflox; Dcrfx; Dicer1fl; Dicerflox; Dicerlox; dicerfl;
Mutation Made By Brian D. Harfe and Mike McManus,   UFlorida and UCSF
Strain of Origin129
ES Cell Line Strain129
Gene Symbol and Name Dicer1, dicer 1, ribonuclease type III
Chromosome 12
Gene Common Name(s) 1110006F08Rik; D12Ertd7e; DCR1; DNA segment, Chr 12, ERATO Doi 7, expressed; Dicer; Dicer1, Dcr-1 homolog (Drosophila); HERNA; MNG1; RIKEN cDNA 1110006F08 gene; RMSE2; mKIAA0928;
Molecular Note A targeting vector was designed to insert loxP sites around the exon that encodes most of the second RNaseIII domain as well as an frt-flanked neo within the loxP-encompassed sequence. Cre-mediated removal of the sequence would result in the loss of 90 amino acids, a loss that does not create a downstream frame-shift or an unstable protein. [MGI Ref ID J:100475]

Genotyping

Genotyping Information

Genotyping Protocols

Dicer1tm1Bdh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Harfe BD; McManus MT; Mansfield JH; Hornstein E; Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A 102(31):10898-903. [PubMed: 16040801]  [MGI Ref ID J:100475]

Additional References

Dicer1tm1Bdh related

Alemdehy MF; van Boxtel NG; de Looper HW; van den Berge IJ; Sanders MA; Cupedo T; Touw IP; Erkeland SJ. 2012. Dicer1 deletion in myeloid-committed progenitors causes neutrophil dysplasia and blocks macrophage/dendritic cell development in mice. Blood 119(20):4723-30. [PubMed: 22353998]  [MGI Ref ID J:185005]

Barritt LC; Miller JM; Scheetz LR; Gardner K; Pierce ML; Soukup GA; Rocha-Sanchez SM. 2012. Conditional deletion of the human ortholog gene Dicer1 in Pax2-Cre expression domain impairs orofacial development. Indian J Hum Genet 18(3):310-9. [PubMed: 23716939]  [MGI Ref ID J:200017]

Belver L; de Yebenes VG; Ramiro AR. 2010. MicroRNAs prevent the generation of autoreactive antibodies. Immunity 33(5):713-22. [PubMed: 21093320]  [MGI Ref ID J:167003]

Beyer M; Thabet Y; Muller RU; Sadlon T; Classen S; Lahl K; Basu S; Zhou X; Bailey-Bucktrout SL; Krebs W; Schonfeld EA; Bottcher J; Golovina T; Mayer CT; Hofmann A; Sommer D; Debey-Pascher S; Endl E; Limmer A; Hippen KL; Blazar BR; Balderas R; Quast T; Waha A; Mayer G; Famulok M; Knolle PA; Wickenhauser C; Kolanus W; Schermer B; Bluestone JA; Barry SC; Sparwasser T; Riley JL; Schultze JL. 2011. Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. Nat Immunol 12(9):898-907. [PubMed: 21841785]  [MGI Ref ID J:176464]

Bezman NA; Cedars E; Steiner DF; Blelloch R; Hesslein DG; Lanier LL. 2010. Distinct requirements of microRNAs in NK cell activation, survival, and function. J Immunol 185(7):3835-46. [PubMed: 20805417]  [MGI Ref ID J:164317]

Biton M; Levin A; Slyper M; Alkalay I; Horwitz E; Mor H; Kredo-Russo S; Avnit-Sagi T; Cojocaru G; Zreik F; Bentwich Z; Poy MN; Artis D; Walker MD; Hornstein E; Pikarsky E; Ben-Neriah Y. 2011. Epithelial microRNAs regulate gut mucosal immunity via epithelium-T cell crosstalk. Nat Immunol 12(3):239-46. [PubMed: 21278735]  [MGI Ref ID J:169306]

Bjorkgren I; Saastamoinen L; Krutskikh A; Huhtaniemi I; Poutanen M; Sipila P. 2012. Dicer1 ablation in the mouse epididymis causes dedifferentiation of the epithelium and imbalance in sex steroid signaling. PLoS One 7(6):e38457. [PubMed: 22701646]  [MGI Ref ID J:187838]

Bremer J; O'Connor T; Tiberi C; Rehrauer H; Weis J; Aguzzi A. 2010. Ablation of Dicer from murine Schwann cells increases their proliferation while blocking myelination. PLoS One 5(8):e12450. [PubMed: 20805985]  [MGI Ref ID J:164021]

Budde H; Schmitt S; Fitzner D; Opitz L; Salinas-Riester G; Simons M. 2010. Control of oligodendroglial cell number by the miR-17-92 cluster. Development 137(13):2127-32. [PubMed: 20504959]  [MGI Ref ID J:161956]

Calabrese JM; Seila AC; Yeo GW; Sharp PA. 2007. RNA sequence analysis defines Dicer's role in mouse embryonic stem cells. Proc Natl Acad Sci U S A 104(46):18097-102. [PubMed: 17989215]  [MGI Ref ID J:127467]

Chang YF; Lee-Chang JS; Imam JS; Buddavarapu KC; Subaran SS; Sinha-Hikim AP; Gorospe M; Rao MK. 2012. Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation. Proc Natl Acad Sci U S A 109(15):5750-5. [PubMed: 22447776]  [MGI Ref ID J:183553]

Chen JA; Huang YP; Mazzoni EO; Tan GC; Zavadil J; Wichterle H. 2011. Mir-17-3p controls spinal neural progenitor patterning by regulating Olig2/Irx3 cross-repressive loop. Neuron 69(4):721-35. [PubMed: 21338882]  [MGI Ref ID J:174745]

Chen JA; Wichterle H. 2012. Apoptosis of limb innervating motor neurons and erosion of motor pool identity upon lineage specific dicer inactivation. Front Neurosci 6:69. [PubMed: 22629237]  [MGI Ref ID J:190166]

Cheung TH; Quach NL; Charville GW; Liu L; Park L; Edalati A; Yoo B; Hoang P; Rando TA. 2012. Maintenance of muscle stem-cell quiescence by microRNA-489. Nature 482(7386):524-8. [PubMed: 22358842]  [MGI Ref ID J:181629]

Chong MM; Rasmussen JP; Rundensky AY; Littman DR. 2008. The RNAseIII enzyme Drosha is critical in T cells for preventing lethal inflammatory disease. J Exp Med 205(9):2005-17. [PubMed: 18725527]  [MGI Ref ID J:138683]

Chong MM; Zhang G; Cheloufi S; Neubert TA; Hannon GJ; Littman DR. 2010. Canonical and alternate functions of the microRNA biogenesis machinery. Genes Dev 24(17):1951-60. [PubMed: 20713509]  [MGI Ref ID J:163823]

Chu JY; Sims-Lucas S; Bushnell DS; Bodnar AJ; Kreidberg JA; Ho J. 2014. Dicer function is required in the metanephric mesenchyme for early kidney development. Am J Physiol Renal Physiol 306(7):F764-72. [PubMed: 24500693]  [MGI Ref ID J:208428]

Cuellar TL; Davis TH; Nelson PT; Loeb GB; Harfe BD; Ullian E; McManus MT. 2008. Dicer loss in striatal neurons produces behavioral and neuroanatomical phenotypes in the absence of neurodegeneration. Proc Natl Acad Sci U S A 105(14):5614-9. [PubMed: 18385371]  [MGI Ref ID J:133780]

Damiani D; Alexander JJ; O'Rourke JR; McManus M; Jadhav AP; Cepko CL; Hauswirth WW; Harfe BD; Strettoi E. 2008. Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina. J Neurosci 28(19):4878-87. [PubMed: 18463241]  [MGI Ref ID J:135193]

Davis N; Mor E; Ashery-Padan R. 2011. Roles for Dicer1 in the patterning and differentiation of the optic cup neuroepithelium. Development 138(1):127-38. [PubMed: 21138975]  [MGI Ref ID J:180843]

Davis TH; Cuellar TL; Koch SM; Barker AJ; Harfe BD; McManus MT; Ullian EM. 2008. Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus. J Neurosci 28(17):4322-30. [PubMed: 18434510]  [MGI Ref ID J:134779]

Dorval V; Smith PY; Delay C; Calvo E; Planel E; Zommer N; Buee L; Hebert SS. 2012. Gene network and pathway analysis of mice with conditional ablation of Dicer in post-mitotic neurons. PLoS One 7(8):e44060. [PubMed: 22952873]  [MGI Ref ID J:191653]

Dridi S; Hirano Y; Tarallo V; Kim Y; Fowler BJ; Ambati BK; Bogdanovich S; Chiodo VA; Hauswirth WW; Kugel JF; Goodrich JA; Ponicsan SL; Hinton DR; Kleinman ME; Baffi JZ; Gelfand BD; Ambati J. 2012. ERK1/2 activation is a therapeutic target in age-related macular degeneration. Proc Natl Acad Sci U S A 109(34):13781-6. [PubMed: 22869729]  [MGI Ref ID J:188603]

Friedman LM; Dror AA; Mor E; Tenne T; Toren G; Satoh T; Biesemeier DJ; Shomron N; Fekete DM; Hornstein E; Avraham KB. 2009. MicroRNAs are essential for development and function of inner ear hair cells in vertebrates. Proc Natl Acad Sci U S A 106(19):7915-20. [PubMed: 19416898]  [MGI Ref ID J:148395]

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da Costa Martins PA; Bourajjaj M; Gladka M; Kortland M; van Oort RJ; Pinto YM; Molkentin JD; De Windt LJ. 2008. Conditional dicer gene deletion in the postnatal myocardium provokes spontaneous cardiac remodeling. Circulation 118(15):1567-76. [PubMed: 18809798]  [MGI Ref ID J:161267]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, mutant mice can be bred to C57BL/6J or bred together to create a homozygous colony.
Mating SystemHomozygote x Homozygote         (Female x Male)   28-JAN-09
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHomozygous for Dicer1tm1Bdh  
Price per Pair (US dollars $)Pair Genotype
$478.00Homozygous for Dicer1tm1Bdh x Homozygous for Dicer1tm1Bdh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHomozygous for Dicer1tm1Bdh  
Price per Pair (US dollars $)Pair Genotype
$621.40Homozygous for Dicer1tm1Bdh x Homozygous for Dicer1tm1Bdh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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