Strain Name: |
B6.Cg-Dicer1tm1Bdh/J |
|---|---|
Stock Number: |
006366 |
Availability: | Under Development for Distribution Colony |
| To register your interest in this strain go to the Strain Interest Form. |
General Terms and Conditions |
| Genes & Alleles | Dicer1; Dicer1tm1Bdh; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Brian Harfe, University of Florida Generation N4 (23-APR-08) Strain Description
These mice contain loxP sites on either side of exon 23. Homozygous mice are viable and fertile with no gross phenotypic or behavioral abnormalities. Expression of the targeted allele is indistinguishable from wildtype despite the frt-flanked neomycin cassette. Cre-mediated recombination (resulting in deletion of exon 23) in the germline leads to developmental arrest at embryonic day 7.5 (E7.5). Tissue specific deletion has been shown to result in loss of microRNA (miRNA) processing. Mutant mice can be used to generate cell/tissue-specific deletions of the endogenous gene for applications in embryonic development, translation, protein processing and miRNA/siRNA regulation of gene expression.For example, when crossed to a strain expressing Cre recombinase in mesenchyme (see Stock No. 005584), this mutant mouse strain may be useful in studies of limb morphogenesis.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector was used to flank exon 23 (encoding most of the second RNaseIII domain) of the endogenous gene with loxP sites. The vector also contained an frt-flanked neomycin resistance cassette between the exon and downstream loxP site. This construct was electroporated into ?129? embryonic stem (ES) cells. Correctly targeted cells were injected into C57BL/6J blastocysts and chimeric mice were bred to C57BL/6J for germline transmission. Heterozygous offspring were bred to generate homozygotes. At some point, homozygotes were bred to mutant Gt(ROSA)26Sor mice on an unknown background. Upon arrival at The Jackson Laboratory, mice on the mixed genetic background were bred to select for the "Dicer-flox" allele and against the Gt(ROSA)26Sor reporter allele, resulting in Stock No. 006001. Mice from that colony were subsequently backcrossed to C57BL/6J for at least 5 generations to create these mice (Stock No. 006366).
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Dicer1tm1Bdh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Brian D Harfe | ||
| Common Name(s) | Dcrflox; Dicerflox; | ||
| Mutation Made By | Brian D. Harfe and Mike McManus, UFlorida and UCSF | ||
| Gene Symbol and Name | Dicer1, Dicer1, Dcr-1 homolog (Drosophila) | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | 1110006F08Rik; D12Ertd7e; DCR1; DNA segment, Chr 12, ERATO Doi 7, expressed; Dicer; HERNA; KIAA0928; RIKEN cDNA 1110006F08 gene; mKIAA0928; | ||
| Molecular Note | A targeting vector was designed to insert loxP sites around the exon that encodes most of the second RNaseIII domain as well as an frt-flanked neo within the loxP-encompassed sequence. Cre-mediated removal of the sequence would result in the loss of 90 amino acids, a loss that does not create a downstream frame-shift or an unstable protein. [MGI Ref ID J:100475] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Dicer1tm1Bdh
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, mutant mice can be bred to C57BL/6J or bred together to create a homozygous colony. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Dicer1tm1Bdh allele
006001 STOCK Dicer1tm1Bdh/J View Strains carrying Dicer1tm1Bdh (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
Cell Biology Research
Protein Processing
Transcriptional Regulation
Research Tools
Cre-lox System (loxP-flanked Sequences)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: transcriptional activation)
References
Selected Reference(s)
Additional ReferencesHarfe BD; McManus MT; Mansfield JH; Hornstein E; Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A 102(31):10898-903. [PubMed: 16040801] [MGI Ref ID J:100475]
Price and Supply Information
| Strain Name: | B6.Cg-Dicer1tm1Bdh/J |
| Stock Number: | 006366 |
This strain is currently Under Development for Distribution Colony.
To register your interest in this strain go to the Strain Interest Form.
Estimated Available for Sale Date: 22-SEP-08
Please note: Estimated available for sale dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for sale depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain/interest registered.
View All Strains Under Development
Supply Details
| Standard Supply | Under Development for Distribution Colony |
|---|---|
| Supply Notes |
This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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