Strain Name: |
B6.Cg-Tg(Cr2-cre)3Cgn/J |
|---|---|
Stock Number: |
006368 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Cr2; cre; Tg(Cr2-cre)3Cgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Stock Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Klaus Rajewsky, Immune Disease Institute (formerly CBRI) Generation N10+N2F3 (01-MAY-08) Strain Description
Mice homozygous for this "CD21-cre3a" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse complement receptor 2 (CR2) promoter. Cre recombinase expression is detected specifically in mature transitional B cells. When CD21-cre3a mice were crossed to a lacZ-expressing Cre reporter strain, Cre recombinase activity is detected in 65-70% of mature B cells (as determined by FACS analysis of bone marrow cells). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in mature B lymphocytes and follicular dendritic cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study B lymphocyte development.Strain Development
A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse complement receptor 2 (CR2) promoter was microinjected into donor eggs of unknown genetic background. Founder line 3 was established and backcrossed to the C57BL/6 background for 10 generations. These "CD21-cre3a" mice were made homozygous prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Cr2-cre)3Cgn | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, University of Cologne | ||
| Common Name(s) | CD21-cre; CD21-cre3a; Cr2tm1(cre)Cgn; | ||
| Mutation Made By | Marat Alimzhanov, CBR Institute for Biomedical Research | ||
| Site of Expression | mature transitional B cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cr2, complement receptor 2, mouse, laboratory | ||
| Molecular Note | This transgene consists of a cre expression cassette including a Kozak sequence, nuclear localization signal, and bovine growth hormone polyadenylation signal inserted into a BAC containing the Cr2 locus. The ATG in the first exon of Cr2 is also mutatedin this allele. Cre mediated recombination is detected in 60% - 80% of mature B cells and is undetectable in pro/pre-B and immature B cells. [MGI Ref ID J:91091] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (115 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesKraus M; Alimzhanov MB; Rajewsky N; Rajewsky K. 2004. Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer. Cell 117(6):787-800. [PubMed: 15186779] [MGI Ref ID J:91091]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Cr2-cre)3Cgn/J |
| Stock Number: | 006368 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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