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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 26-APR-08 Species laboratory mouse Generation N10+N2F10 (29-JUN-11)
Generation DefinitionsDonating Investigator Klaus Rajewsky, Immune Disease Institute (formerly CBRI) Description
Mice homozygous for this "CD21-cre3a" transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse complement receptor 2 (CR2) promoter. Cre recombinase expression is detected specifically in mature transitional B cells. When CD21-cre3a mice were crossed to a lacZ-expressing Cre reporter strain, Cre recombinase activity is detected in 65-70% of mature B cells (as determined by FACS analysis of bone marrow cells). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in mature B lymphocytes and follicular dendritic cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study B lymphocyte development.Development
A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse complement receptor 2 (CR2) promoter was microinjected into donor eggs of unknown genetic background. Founder line 3 was established and backcrossed to the C57BL/6 background for 10 generations. These "CD21-cre3a" mice were made homozygous prior to arrival at The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (311 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Immunology and Inflammation Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Cr2-cre)3Cgn | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, University of Cologne | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | CD21-cre; CD21-cre3a; Cr2tm1(cre)Cgn; | ||
| Mutation Made By | Marat Alimzhanov, CBR Institute for Biomedical Research | ||
| Site of Expression | mature transitional B cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cr2, complement receptor 2, mouse, laboratory | ||
| Driver Note | Cr2 | ||
| Molecular Note | This transgene consists of a cre expression cassette including a Kozak sequence, nuclear localization signal, and bovine growth hormone polyadenylation signal inserted into a BAC containing the Cr2 locus. The ATG in the first exon of Cr2 is also mutatedin this allele. Cre mediated recombination is detected in 60% - 80% of mature B cells and is undetectable in pro/pre-B and immature B cells. [MGI Ref ID J:91091] | ||
| Gene Symbol and Name | Tg(Cr2-cre)3Cgn, transgene insertion 3, University of Cologne | ||
| Chromosome | UN | ||
| Gene Common Name(s) | CD21-cre; CD21-cre3a; Cr2tm1(cre)Cgn; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Kraus M; Alimzhanov MB; Rajewsky N; Rajewsky K. 2004. Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer. Cell 117(6):787-800. [PubMed: 15186779] [MGI Ref ID J:91091]
Additional References
Tg(Cr2-cre)3Cgn relatedBoboila C; Jankovic M; Yan CT; Wang JH; Wesemann DR; Zhang T; Fazeli A; Feldman L; Nussenzweig A; Nussenzweig M; Alt FW. 2010. Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70. Proc Natl Acad Sci U S A 107(7):3034-9. [PubMed: 20133803] [MGI Ref ID J:157537]
Boboila C; Yan C; Wesemann DR; Jankovic M; Wang JH; Manis J; Nussenzweig A; Nussenzweig M; Alt FW. 2010. Alternative end-joining catalyzes class switch recombination in the absence of both Ku70 and DNA ligase 4. J Exp Med 207(2):417-27. [PubMed: 20142431] [MGI Ref ID J:158825]
Dengler HS; Baracho GV; Omori SA; Bruckner S; Arden KC; Castrillon DH; DePinho RA; Rickert RC. 2008. Distinct functions for the transcription factor Foxo1 at various stages of B cell differentiation. Nat Immunol 9(12):1388-98. [PubMed: 18978794] [MGI Ref ID J:143228]
Eccleston J; Yan C; Yuan K; Alt FW; Selsing E. 2011. Mismatch repair proteins MSH2, MLH1, and EXO1 are important for class-switch recombination events occurring in B cells that lack nonhomologous end joining. J Immunol 186(4):2336-43. [PubMed: 21242524] [MGI Ref ID J:169152]
Gostissa M; Yan CT; Bianco JM; Cogne M; Pinaud E; Alt FW. 2009. Long-range oncogenic activation of Igh-c-myc translocations by the Igh 3' regulatory region. Nature 462(7274):803-7. [PubMed: 20010689] [MGI Ref ID J:155801]
Green JA; Suzuki K; Cho B; Willison LD; Palmer D; Allen CD; Schmidt TH; Xu Y; Proia RL; Coughlin SR; Cyster JG. 2011. The sphingosine 1-phosphate receptor S1P maintains the homeostasis of germinal center B cells and promotes niche confinement. Nat Immunol 12(7):672-80. [PubMed: 21642988] [MGI Ref ID J:174313]
Lam KP; Kuhn R; Rajewsky K. 1997. In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death. Cell 90(6):1073-83. [PubMed: 9323135] [MGI Ref ID J:79299]
Moriyama M; Fukuhara T; Britschgi M; He Y; Narasimhan R; Villeda S; Molina H; Huber BT; Holers M; Wyss-Coray T. 2011. Complement Receptor 2 Is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis. J Neurosci 31(11):3981-3989. [PubMed: 21411641] [MGI Ref ID J:170456]
Oak JS; Chen J; Peralta RQ; Deane JA; Fruman DA. 2009. The p85beta regulatory subunit of phosphoinositide 3-kinase has unique and redundant functions in B cells. Autoimmunity 42(5):447-58. [PubMed: 19811262] [MGI Ref ID J:172476]
Rivera-Munoz P; Soulas-Sprauel P; Le Guyader G; Abramowski V; Bruneau S; Fischer A; Paques F; de Villartay JP. 2009. Reduced immunoglobulin class switch recombination in the absence of Artemis. Blood 114(17):3601-9. [PubMed: 19692705] [MGI Ref ID J:153841]
Schenten D; Kracker S; Esposito G; Franco S; Klein U; Murphy M; Alt FW; Rajewsky K. 2009. Pol zeta ablation in B cells impairs the germinal center reaction, class switch recombination, DNA break repair, and genome stability. J Exp Med 206(2):477-90. [PubMed: 19204108] [MGI Ref ID J:146453]
Srinivasan L; Sasaki Y; Calado DP; Zhang B; Paik JH; DePinho RA; Kutok JL; Kearney JF; Otipoby KL; Rajewsky K. 2009. PI3 kinase signals BCR-dependent mature B cell survival. Cell 139(3):573-86. [PubMed: 19879843] [MGI Ref ID J:157297]
Victoratos P; Kollias G. 2009. Induction of autoantibody-mediated spontaneous arthritis critically depends on follicular dendritic cells. Immunity 30(1):130-42. [PubMed: 19119026] [MGI Ref ID J:143728]
Victoratos P; Lagnel J; Tzima S; Alimzhanov MB; Rajewsky K; Pasparakis M; Kollias G. 2006. FDC-specific functions of p55TNFR and IKK2 in the development of FDC networks and of antibody responses. Immunity 24(1):65-77. [PubMed: 16413924] [MGI Ref ID J:113314]
Vuong BQ; Lee M; Kabir S; Irimia C; Macchiarulo S; McKnight GS; Chaudhuri J. 2009. Specific recruitment of protein kinase A to the immunoglobulin locus regulates class-switch recombination. Nat Immunol 10(4):420-6. [PubMed: 19234474] [MGI Ref ID J:147785]
Wang JH; Gostissa M; Yan CT; Goff P; Hickernell T; Hansen E; Difilippantonio S; Wesemann DR; Zarrin AA; Rajewsky K; Nussenzweig A; Alt FW. 2009. Mechanisms promoting translocations in editing and switching peripheral B cells. Nature 460(7252):231-6. [PubMed: 19587764] [MGI Ref ID J:150339]
Wang X; Cho B; Suzuki K; Xu Y; Green JA; An J; Cyster JG. 2011. Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers. J Exp Med 208(12):2497-510. [PubMed: 22042977] [MGI Ref ID J:178640]
Wu S; Hu YC; Liu H; Shi Y. 2009. Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis. Mol Cell Biol 29(23):6245-56. [PubMed: 19786570] [MGI Ref ID J:155000]
Yan CT; Boboila C; Souza EK; Franco S; Hickernell TR; Murphy M; Gumaste S; Geyer M; Zarrin AA; Manis JP; Rajewsky K; Alt FW. 2007. IgH class switching and translocations use a robust non-classical end-joining pathway. Nature 449(7161):478-82. [PubMed: 17713479] [MGI Ref ID J:126758]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System +/+ sibling x Hemizygote (Female x Male) 26-APR-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Cr2-cre)3Cgn
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Cr2-cre)3Cgn x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Cr2-cre)3Cgn Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Cr2-cre)3Cgn
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Cr2-cre)3Cgn x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Cr2-cre)3Cgn Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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