Strain Name:

B6;129P2-Rims1tm1Sud/J

Stock Number:

006376

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating InvestigatorDr. Thomas C. Sudhof,   Stanford University School of Medicine

Description
Mice homozygous for this targeted mutation are viable and fertile, but are poor breeders. Morphological analyses of the brain failed to uncover structural abnormalities or changes in brain architecture. Protein product from the targeted gene is not detected in brain tissue. No statistically significant difference in the density and size of synapses, synaptic vesicle density, or number of docked vesicles has been detected. Decreased transmitter release during paired stimulations was found at inhibitory synapses, opposite to an increase at excitatory synapses. Post-tetanic potentiation (short-term plasticity) is strongly enhanced. This mutant mouse strain represents a model that may be useful in studies of neurotransmitter release.

Development
Targeting vectors containing a neomycin resistance gene were used to replace the first coding exon of the targeted gene. The constructs were electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 for at least three generations.

Control Information

  Control
   Wild-type from the colony
   100903 B6129PF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Rims1
006384   B6;129-Rims1tm2Sud/J
015832   STOCK Rims1tm3Sud/J
View Strains carrying other alleles of Rims1     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Cone-Rod Dystrophy 7; CORD7   (RIMS1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Rims1tm1Sud/Rims1tm1Sud

        involves: 129P2/OlaHsd * C57BL/6
  • behavior/neurological phenotype
  • abnormal maternal nurturing
    • mothers failed to care for litters   (MGI Ref ID J:73900)
  • nervous system phenotype
  • abnormal CNS synaptic transmission   (MGI Ref ID J:73900)
    • abnormal excitatory postsynaptic potential
      • an overall decreased probability of neurotransmitter release in excitatory synapses   (MGI Ref ID J:73900)
      • magnitude of synaptic responses is decreased   (MGI Ref ID J:73900)
      • no change in neurotransmitter release in inhibitory synapses   (MGI Ref ID J:73900)
    • abnormal long term depression
      • long term depression is increased in mossy fibers of the hippocampus   (MGI Ref ID J:73899)
    • abnormal post-tetanic potentiation   (MGI Ref ID J:73900)
    • reduced long term potentiation
      • long term potentiation is absent in the mossy fibers of the hippocampus and in the cerebellum   (MGI Ref ID J:73899)
  • abnormal paired-pulse facilitation   (MGI Ref ID J:73900)
    • decreased paired-pulse facilitation
      • paired pulse depression seen at inhibitory synapses of CA1 pyramidal neurons   (MGI Ref ID J:73900)
    • enhanced paired-pulse facilitation
      • facilitation was greatly increased in excitatory synapses in the hippocampus with short interstimulation intervals but not with longer ones   (MGI Ref ID J:73900)
      • repetitive stimulation of excitatory synapses produced extended facilitation   (MGI Ref ID J:73900)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Rims1tm1Sud/Rims1tm1Sud

        involves: 129P2/OlaHsd
  • nervous system phenotype
  • abnormal excitatory postsynaptic currents
    • in hippocampal neurons, attenuation of NMDAR-mediated EPSCs by MK801 in mutants is much slower than in wild-type during repetitive stimulation   (MGI Ref ID J:106269)
  • abnormal inhibitory postsynaptic currents
    • miniature inhibitory postsynaptic current (mIPSC) frequency is decreased compared to in wild-type mice   (MGI Ref ID J:143053)
    • reduction in mIPSC is less severe than in Rims1tm3.1Sud homozygotes   (MGI Ref ID J:143053)
    • evoked inhibitory postsynaptic current (eIPSC) amplitude is reduced compared to in wild-type mice   (MGI Ref ID J:143053)
    • reduction in eIPSC is less severe than in Rims1tm3.1Sud homozygotes   (MGI Ref ID J:143053)
  • abnormal paired-pulse inhibition
    • mice exhibit a modest reduction in paired pulse depression compared to in wild-type mice that is less pronounced than in Rims1tm3Sud neurons exposed to a cre-expressing lentivirus   (MGI Ref ID J:143053)
  • disorganized barrel cortex
    • barrels corresponding to the small tactile hairs on the snout are absent or indistinct in nulls in mice aged 8-21 days; in adult mice, barrel boundaries are indistinct in mutants; a uniform distribution of layer IV neurons is observed in mutants instead of being restricted to barrels, except for a few large barrels   (MGI Ref ID J:106269)
  • impaired synaptic plasticity
    • in mutants, paired stimuli produces less paired-pulse depression than in wild-type; paired-pulse ratios are increased in mutants compared to wild-type   (MGI Ref ID J:106269)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Rims1tm1Sud
Allele Name targeted mutation 1, Thomas C Sudhof
Allele Type Targeted (Null/Knockout)
Common Name(s) RIM1alpha-;
Mutation Made ByDr. Thomas Sudhof,   Stanford University School of Medicine
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Rims1, regulating synaptic membrane exocytosis 1
Chromosome 1
Gene Common Name(s) C030033M19Rik; CORD7; RAB3IP2; RIKEN cDNA C030033M19 gene; RIM; RIM1; RIM1a; RIM1alpha;
Molecular Note A neomycin resistance cassette replaced the first coding exon, which encodes the translation initiation site and 55 conserved N-terminal residues. Immunoblot analysis using antibody to the N-terminal zinc finger domain did not detect protein. [MGI Ref ID J:73900]

Genotyping

Genotyping Information

Genotyping Protocols

Rims1tm1Sud, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Schoch S; Castillo PE; Jo T; Mukherjee K; Geppert M; Wang Y; Schmitz F; Malenka RC; Sudhof TC. 2002. RIM1alpha forms a protein scaffold for regulating neurotransmitter release at the active zone. Nature 415(6869):321-6. [PubMed: 11797009]  [MGI Ref ID J:73900]

Additional References

Rims1tm1Sud related

Andrews-Zwilling YS; Kawabe H; Reim K; Varoqueaux F; Brose N. 2006. Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2. J Biol Chem 281(28):19720-31. [PubMed: 16704978]  [MGI Ref ID J:114867]

Blundell J; Kaeser PS; Sudhof TC; Powell CM. 2010. RIM1alpha and interacting proteins involved in presynaptic plasticity mediate prepulse inhibition and additional behaviors linked to schizophrenia. J Neurosci 30(15):5326-33. [PubMed: 20392954]  [MGI Ref ID J:159850]

Calakos N; Schoch S; Sudhof TC; Malenka RC. 2004. Multiple roles for the active zone protein RIM1alpha in late stages of neurotransmitter release. Neuron 42(6):889-96. [PubMed: 15207234]  [MGI Ref ID J:107731]

Castillo PE; Schoch S; Schmitz F; Sudhof TC; Malenka RC. 2002. RIM1alpha is required for presynaptic long-term potentiation. Nature 415(6869):327-30. [PubMed: 11797010]  [MGI Ref ID J:73899]

Chevaleyre V; Heifets BD; Kaeser PS; Sudhof TC; Castillo PE. 2007. Endocannabinoid-mediated long-term plasticity requires cAMP/PKA signaling and RIM1alpha. Neuron 54(5):801-12. [PubMed: 17553427]  [MGI Ref ID J:128627]

Fourcaudot E; Gambino F; Humeau Y; Casassus G; Shaban H; Poulain B; Luthi A. 2008. cAMP/PKA signaling and RIM1alpha mediate presynaptic LTP in the lateral amygdala. Proc Natl Acad Sci U S A 105(39):15130-5. [PubMed: 18815362]  [MGI Ref ID J:143369]

Grueter BA; Brasnjo G; Malenka RC. 2010. Postsynaptic TRPV1 triggers cell type-specific long-term depression in the nucleus accumbens. Nat Neurosci 13(12):1519-25. [PubMed: 21076424]  [MGI Ref ID J:166764]

Huang YY; Zakharenko SS; Schoch S; Kaeser PS; Janz R; Sudhof TC; Siegelbaum SA; Kandel ER. 2005. Genetic evidence for a protein-kinase-A-mediated presynaptic component in NMDA-receptor-dependent forms of long-term synaptic potentiation. Proc Natl Acad Sci U S A 102(26):9365-70. [PubMed: 15967982]  [MGI Ref ID J:99869]

Kaeser PS; Kwon HB; Chiu CQ; Deng L; Castillo PE; Sudhof TC. 2008. RIM1alpha and RIM1beta are synthesized from distinct promoters of the RIM1 gene to mediate differential but overlapping synaptic functions. J Neurosci 28(50):13435-47. [PubMed: 19074017]  [MGI Ref ID J:143053]

Lachamp PM; Liu Y; Liu SJ. 2009. Glutamatergic modulation of cerebellar interneuron activity is mediated by an enhancement of GABA release and requires protein kinase A/RIM1alpha signaling. J Neurosci 29(2):381-92. [PubMed: 19144838]  [MGI Ref ID J:144499]

Lonart G; Tang X; Simsek-Duran F; Machida M; Sanford LD. 2008. The role of active zone protein Rab3 interacting molecule 1 alpha in the regulation of norepinephrine release, response to novelty, and sleep. Neuroscience 154(2):821-31. [PubMed: 18495360]  [MGI Ref ID J:139395]

Lu HC; Butts DA; Kaeser PS; She WC; Janz R; Crair MC. 2006. Role of efficient neurotransmitter release in barrel map development. J Neurosci 26(10):2692-703. [PubMed: 16525048]  [MGI Ref ID J:106269]

Pitsch J; Opitz T; Borm V; Woitecki A; Staniek M; Beck H; Becker AJ; Schoch S. 2012. The Presynaptic Active Zone Protein RIM1alpha Controls Epileptogenesis following Status Epilepticus. J Neurosci 32(36):12384-95. [PubMed: 22956829]  [MGI Ref ID J:187680]

Powell CM; Schoch S; Monteggia L; Barrot M; Matos MF; Feldmann N; Sudhof TC; Nestler EJ. 2004. The presynaptic active zone protein RIM1alpha is critical for normal learning and memory. Neuron 42(1):143-53. [PubMed: 15066271]  [MGI Ref ID J:89753]

Schoch S; Mittelstaedt T; Kaeser PS; Padgett D; Feldmann N; Chevaleyre V; Castillo PE; Hammer RE; Han W; Schmitz F; Lin W; Sudhof TC. 2006. Redundant functions of RIM1alpha and RIM2alpha in Ca(2+)-triggered neurotransmitter release. EMBO J 25(24):5852-63. [PubMed: 17124501]  [MGI Ref ID J:119934]

Yang Y; Calakos N. 2010. Acute in vivo genetic rescue demonstrates that phosphorylation of RIM1alpha serine 413 is not required for mossy fiber long-term potentiation. J Neurosci 30(7):2542-6. [PubMed: 20164339]  [MGI Ref ID J:167890]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   100903 B6129PF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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