Strain Name: |
B6;129P2-Vamp2tm1Sud/J |
|---|---|
Stock Number: |
006380 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Vamp2; Vamp2tm1Sud; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Thomas Sudhof, Stanford University School of Medicine Generation ?+N1F0 (25-JUL-07) Strain Description
Mice homozygous for this targeted mutation die shortly after birth. Heterozygotes suffer no apparent morbidity or premature mortality. Newborn homozygotes exhibit a rounded appearance with a shoulder hump that may be caused by excess brown fat in the upper back. No developmental changes were noted. Analysis of brain sections did not detect any abnormalities or neurodegeneration. No changes are observed in the expression of a series of synaptic proteins. Spontaneous synaptic vesicle fusion and fusion induced by hypertonic sucrose are decreased approximately 10-fold, and fast Ca2+-triggered fusion was decreased more than 100-fold. No protein product from the targeted gene is detected in forebrain tissue. This mutant mouse strain represents a model that may be useful in studies of synaptic fusion mechanisms.Strain Development
A targeting vector containing a floxed neomycin resistance gene was used to disrupt sequence 104 bp upstream of the initiator ATG. Point mutations (V38R and V42R) were introduced into the coding region. The construct was electroporated into 129P2/OlaHsd –derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric mice were crossed with C57BL/6 mice. The strain has been backcrossed to C57BL/6 for several generations by the donating laboratory.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Vamp2tm1Sud | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Thomas C Sudhof | ||
| Common Name(s) | syb 2 -/-; synaptobrevin/VAMP2-; | ||
| Mutation Made By | Thomas Sudhof, Stanford University School of Medicine | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Vamp2, vesicle-associated membrane protein 2 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | FLJ11460; RATVAMPB; RATVAMPIR; SYB; SYB2; Syb-2; Syb2; VAMP-2; synaptobrevin 2; synaptobrevin II; | ||
| Molecular Note | A loxP-flanked neomycin resistance cassette was inserted 104 bp upstream of the initiation codon. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable protein is produced from this allele. [MGI Ref ID J:72714] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 100903 B6129PF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Vamp2
008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J View Strains carrying other alleles of Vamp2 (1 strain)
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
References
Selected Reference(s)
Additional ReferencesSchoch S; Deak F; Konigstorfer A; Mozhayeva M; Sara Y; Sudhof TC; Kavalali ET. 2001. SNARE function analyzed in synaptobrevin/VAMP knockout mice. Science 294(5544):1117-22. [PubMed: 11691998] [MGI Ref ID J:72714]
Price and Supply Information
| Strain Name: | B6;129P2-Vamp2tm1Sud/J |
| Stock Number: | 006380 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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