Strain Name:

B6;129P2-Vamp2tm1Sud/J

Stock Number:

006380

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating InvestigatorDr. Thomas C. Sudhof,   Stanford University School of Medicine

Description
Mice homozygous for this targeted mutation die shortly after birth. Heterozygotes suffer no apparent morbidity or premature mortality. Newborn homozygotes exhibit a rounded appearance with a shoulder hump that may be caused by excess brown fat in the upper back. No developmental changes were noted. Analysis of brain sections did not detect any abnormalities or neurodegeneration. No changes are observed in the expression of a series of synaptic proteins. Spontaneous synaptic vesicle fusion and fusion induced by hypertonic sucrose are decreased approximately 10-fold, and fast Ca2+-triggered fusion was decreased more than 100-fold. No protein product from the targeted gene is detected in forebrain tissue. This mutant mouse strain represents a model that may be useful in studies of synaptic fusion mechanisms.

Development
A targeting vector containing a floxed neomycin resistance gene was used to disrupt sequence 104 bp upstream of the initiator ATG. Point mutations (V38R and V42R) were introduced into the coding region. The construct was electroporated into 129P2/OlaHsd –derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric mice were crossed with C57BL/6 mice. The strain has been backcrossed to C57BL/6 for several generations by the donating laboratory.

Control Information

  Control
   Wild-type from the colony
   100903 B6129PF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Vamp2
008531   B6;129-Vamp2tm1(cre/ERT)Nat/J
014651   B6;CBA-Tg(Thy1-spH)21Vnmu/J
015814   B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ
View Strains carrying other alleles of Vamp2     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Vamp2tm1Sud/Vamp2+

        involves: 129P2/OlaHsd * C57BL/6J
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • extent and time course of thrombin-induced secretion is comparable to wild-type   (MGI Ref ID J:121010)

Vamp2tm1Sud/Vamp2tm1Sud

        involves: 129P2/OlaHsd * C57BL/6
  • mortality/aging
  • complete neonatal lethality
    • animals die immediately after birth   (MGI Ref ID J:72714)
  • growth/size/body phenotype
  • abnormal postnatal growth/weight/body size
    • rounded, curled appearance at birth   (MGI Ref ID J:72714)
  • nervous system phenotype
  • abnormal miniature excitatory postsynaptic currents
    • reduced frequency (about 15% of controls) of spontaneous miniature excitatory currents in hippocampal neurons   (MGI Ref ID J:72714)
    • single neurons stimulated at 1 Hz exhibit only rare synaptic events with small amplitudes corresponding to minis; even at high frequencies, only erratic events occur that are not completely synchronous with the stimulation   (MGI Ref ID J:72714)
  • decreased neurotransmitter release
    • spontaneous synaptic vesicle fusion and fusion induced by hypertonic sucrose is decreased about 10-fold and fast calcium-triggered fusion is decreased more than 100-fold, indicating that neurotransmitter release is operational but cannot be efficiently stimulated by calcium   (MGI Ref ID J:72714)
  • immune system phenotype
  • *normal* immune system phenotype
    • homozygotes exhibit normal degranulation of mast cells   (MGI Ref ID J:131926)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Vamp2tm1Sud/Vamp2tm1Sud

        involves: 129P2/OlaHsd
  • endocrine/exocrine gland phenotype
  • abnormal adrenal chromaffin cell morphology
    • total number of chromaffin vesicles is increased compared to Stx1a mutants   (MGI Ref ID J:138035)
  • nervous system phenotype
  • abnormal adrenal chromaffin cell morphology
    • total number of chromaffin vesicles is increased compared to Stx1a mutants   (MGI Ref ID J:138035)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Vamp2tm1Sud
Allele Name targeted mutation 1, Thomas C Sudhof
Allele Type Targeted (knock-out)
Common Name(s) syb 2 -; synaptobrevin/VAMP2-;
Mutation Made ByDr. Thomas Sudhof,   Stanford University School of Medicine
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Vamp2, vesicle-associated membrane protein 2
Chromosome 11
Gene Common Name(s) RATVAMPB; RATVAMPIR; SYB; SYB2; Syb-2; Syb2; VAMP-2; sybII; synaptobrevin 2; synaptobrevin II;
Molecular Note A loxP-flanked neomycin resistance cassette was inserted 104 bp upstream of the initiation codon. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable protein is produced from this allele. [MGI Ref ID J:72714]

Genotyping

Genotyping Information

Genotyping Protocols

Vamp2tm1Sudalternate3, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Schoch S; Deak F; Konigstorfer A; Mozhayeva M; Sara Y; Sudhof TC; Kavalali ET. 2001. SNARE function analyzed in synaptobrevin/VAMP knockout mice. Science 294(5544):1117-22. [PubMed: 11691998]  [MGI Ref ID J:72714]

Additional References

Vamp2tm1Sud related

Borisovska M; Zhao Y; Tsytsyura Y; Glyvuk N; Takamori S; Matti U; Rettig J; Sudhof T; Bruns D. 2005. v-SNAREs control exocytosis of vesicles from priming to fusion. EMBO J 24(12):2114-26. [PubMed: 15920476]  [MGI Ref ID J:100913]

Deak F; Shin OH; Kavalali ET; Sudhof TC. 2006. Structural determinants of synaptobrevin 2 function in synaptic vesicle fusion. J Neurosci 26(25):6668-76. [PubMed: 16793874]  [MGI Ref ID J:109985]

Fang Q; Zhao Y; Lindau M. 2013. Juxtamembrane tryptophans of synaptobrevin 2 control the process of membrane fusion. FEBS Lett 587(1):67-72. [PubMed: 23178715]  [MGI Ref ID J:192579]

Gerber SH; Rah JC; Min SW; Liu X; de Wit H; Dulubova I; Meyer AC; Rizo J; Arancillo M; Hammer RE; Verhage M; Rosenmund C; Sudhof TC. 2008. Conformational Switch of Syntaxin-1 Controls Synaptic Vesicle Fusion. Science 321(5895):1507-10. [PubMed: 18703708]  [MGI Ref ID J:138035]

Lambertsen KL; Gramsbergen JB; Sivasaravanaparan M; Ditzel N; Sevelsted-Moller LM; Olivan-Viguera A; Rabjerg M; Wulff H; Kohler R. 2012. Genetic KCa3.1-deficiency produces locomotor hyperactivity and alterations in cerebral monoamine levels. PLoS One 7(10):e47744. [PubMed: 23077667]  [MGI Ref ID J:192205]

Matveeva EA; Price DA; Whiteheart SW; Vanaman TC; Gerhardt GA; Slevin JT. 2012. Reduction of vesicle-associated membrane protein 2 expression leads to a kindling-resistant phenotype in a murine model of epilepsy. Neuroscience 202:77-86. [PubMed: 22183055]  [MGI Ref ID J:184394]

Ngatchou AN; Kisler K; Fang Q; Walter AM; Zhao Y; Bruns D; Sorensen JB; Lindau M. 2010. Role of the synaptobrevin C terminus in fusion pore formation. Proc Natl Acad Sci U S A 107(43):18463-8. [PubMed: 20937897]  [MGI Ref ID J:165505]

Puri N; Roche PA. 2008. Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms. Proc Natl Acad Sci U S A 105(7):2580-5. [PubMed: 18250339]  [MGI Ref ID J:131926]

Raingo J; Khvotchev M; Liu P; Darios F; Li YC; Ramirez DM; Adachi M; Lemieux P; Toth K; Davletov B; Kavalali ET. 2012. VAMP4 directs synaptic vesicles to a pool that selectively maintains asynchronous neurotransmission. Nat Neurosci 15(5):738-45. [PubMed: 22406549]  [MGI Ref ID J:191257]

Ren Q; Barber HK; Crawford GL; Karim ZA; Zhao C; Choi W; Wang CC; Hong W; Whiteheart SW. 2007. Endobrevin/VAMP-8 is the primary v-SNARE for the platelet release reaction. Mol Biol Cell 18(1):24-33. [PubMed: 17065550]  [MGI Ref ID J:121010]

Sando R 3rd; Gounko N; Pieraut S; Liao L; Yates J 3rd; Maximov A. 2012. HDAC4 governs a transcriptional program essential for synaptic plasticity and memory. Cell 151(4):821-34. [PubMed: 23141539]  [MGI Ref ID J:193206]

Zhou P; Bacaj T; Yang X; Pang ZP; Sudhof TC. 2013. Lipid-anchored SNAREs lacking transmembrane regions fully support membrane fusion during neurotransmitter release. Neuron 80(2):470-83. [PubMed: 24120845]  [MGI Ref ID J:207214]

Zylbersztejn K; Petkovic M; Burgo A; Deck M; Garel S; Marcos S; Bloch-Gallego E; Nothias F; Serini G; Bagnard D; Binz T; Galli T. 2012. The vesicular SNARE Synaptobrevin is required for Semaphorin 3A axonal repulsion. J Cell Biol 196(1):37-46. [PubMed: 22213797]  [MGI Ref ID J:180658]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes are bred with wildtype siblings. Homozygotes are not viable.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   100903 B6129PF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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