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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator Dr. Thomas C. Sudhof, Stanford University School of Medicine Description
Mice homozygous for this targeted point mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. The mutant gene is fully expressed. A two-fold decrease in the overall Ca2+ affinity of the protein and a corresponding decrease in the Ca2+ sensitivity of neurotransmitter release is observed. Nuclear Magnetic Resonance (NMR) spectroscopy shows no structural or conformational changes in the mutant protein. This mutant mouse strain represents a model that may be useful in studies of synaptic neurotransmitter release.Development
A targeting vector was used to create an R233Q substitution in the exon encoding residues 214-269 and insert a floxed neomycin resistance gene into the flanking intron. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6. The strain has been minimally backcrossed to C57BL/6 by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 100903 B6129PF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Syt1
006386 B6;129P2-Syt1tm5Sud/J 006387 B6;129P2-Syt1tm6Sud/J 002478 B6;129S-Syt1tm1Sud/J View Strains carrying other alleles of Syt1 (3 strains)
Phenotype
Phenotype Information
assigned by genotype
Syt1tm3Sud/Syt1tm3Sud
involves: 129P2/OlaHsd * C57BL/6
- nervous system phenotype
- abnormal excitatory postsynaptic currents
- the ESPC amplitudes of mutant neurons were smaller than controls during repetitive stimulation; facilitation of the response was seen at various frequencies whereas synaptic depression was seen in controls (MGI Ref ID J:76452)
- abnormal neurotransmitter secretion
- the fast, synchronous, Ca2+ dependent phase of neurotransmitter release is affected, while the spontaneous release of neurotransmitters is unaffected (MGI Ref ID J:76452)
- the Ca2+ dependent synaptic release probability was decreased two-fold, but was not due to a decrease in the readily releasable pool of neurotransmitters (MGI Ref ID J:76452)
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Syt1tm3Sud | ||
|---|---|---|---|
| Allele Name | targeted mutation 3, Thomas C Sudhof | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | R233Q; Syt1R233Q+; | ||
| Mutation Made By | Dr. Thomas Sudhof, Stanford University School of Medicine | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Promoter | Syt1, synaptotagmin I, mouse, laboratory | ||
| Molecular Note | Insertion of a neomycin resistance cassette into intron 1 and an arginine to glutamine point mutation at position 233 of exon 1 (R233Q) was introduced via homologous recombination. Western blot of brain homogenates from homozygous mutant animals verifiedthe presence of protein expression and enzyme assays demonstrated similar phospholipid- and syntaxin-binding ability compared to wild-type. However, calcium affinity was decreased by 2-fold. [MGI Ref ID J:76452] | ||
References
References provided by MGI
Selected Reference(s)
Fernandez-Chacon R; Konigstorfer A; Gerber SH; Garcia J; Matos MF; Stevens CF; Brose N; Rizo J; Rosenmund C; Sudhof TC. 2001. Synaptotagmin I functions as a calcium regulator of release probability. Nature 410(6824):41-9. [PubMed: 11242035] [MGI Ref ID J:76452]
Additional References
Syt1tm3Sud relatedBlundell J; Kaeser PS; Sudhof TC; Powell CM. 2010. RIM1alpha and interacting proteins involved in presynaptic plasticity mediate prepulse inhibition and additional behaviors linked to schizophrenia. J Neurosci 30(15):5326-33. [PubMed: 20392954] [MGI Ref ID J:159850]
Powell CM; Schoch S; Monteggia L; Barrot M; Matos MF; Feldmann N; Sudhof TC; Nestler EJ. 2004. The presynaptic active zone protein RIM1alpha is critical for normal learning and memory. Neuron 42(1):143-53. [PubMed: 15066271] [MGI Ref ID J:89753]
Sorensen JB; Fernandez-Chacon R; Sudhof TC; Neher E. 2003. Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2+. J Gen Physiol 122(3):265-76. [PubMed: 12939392] [MGI Ref ID J:105824]
Xu J; Pang ZP; Shin OH; Sudhof TC. 2009. Synaptotagmin-1 functions as a Ca2+ sensor for spontaneous release. Nat Neurosci 12(6):759-66. [PubMed: 19412166] [MGI Ref ID J:161265]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintained as a live colony, homozygous breeders are crossed.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 100903 B6129PF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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