Strain Name: |
B6;129-Syt7tm1Sud/J |
|---|---|
Stock Number: |
006388 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.129-Syt7tm1Sud/J (Changed: 30-JUL-08
) |
| Genes & Alleles | Syt7; Syt7tm1Sud; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Stock Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Heterozygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Thomas Sudhof, U. of Texas, SW Medical Center at Dallas Generation N5+N1F1 (04-JAN-08) Strain Description
Homozygotes are viable, fertile, and have normal body weight. There is no obvious neuronal phenotype. Western analysis indicates that no protein product is produced.Strain Development
A targeting vector containing point mutations D225A, D227A, and D233A in exon 12, and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene in the intervening intron was produced. The vector was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 and the foundation line has been backcrossed at least five times to C57BL/6 by the donating laboratory.NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed genetic background (9 out of 27 markers are segregating for non-C57BL/6 alleles). July, 2008.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Syt7tm1Sud | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Thomas C Sudhof | ||
| Mutation Made By | Thomas Sudhof, U. of Texas, SW Medical Center at Dallas | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Syt7, synaptotagmin VII | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | AI851541; B230112P13Rik; IPCA-7; MGC150517; PCANAP7; RIKEN cDNA B230112P13 gene; SYT-VII; expressed sequence AI851541; | ||
| Molecular Note | Point mutations D225A, D227A, and D233A in exon 12, and mutations D303A, D357A, and D359A in exon 13, along with a floxed neomycin resistance gene in the intervening intron were introduced in the Syt7 locus. No protein is detected by Western blot. [MGI Ref ID J:118318] [MGI Ref ID J:131423] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Syt7tm1Sud, tm2Sud
Colony Maintenance
| Breeding & Husbandry | When maintained as a live colony, heterozygotes or homozygotes may be bred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Syt7
004950 B6.129S1-Syt7tm1Nan/J 006389 B6;129-Syt7tm2Sud/J View Strains carrying other alleles of Syt7 (2 strains)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
References
Selected Reference(s)
Additional ReferencesMaximov A; Lao Y; Li H; Chen X; Rizo J; Sorensen JB; Sudhof TC. 2008. Genetic analysis of synaptotagmin-7 function in synaptic vesicle exocytosis. Proc Natl Acad Sci U S A :. [PubMed: 18308933] [MGI Ref ID J:131423]
Price and Supply Information
| Strain Name: | B6;129-Syt7tm1Sud/J |
| Stock Number: | 006388 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form