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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Former Names B6.129-Syt7tm2Sud/J (Changed: 30-JUL-08 ) B6.129-Syt7tm1.1Sud/J (Changed: 21-MAR-07 ) Type Mutant Stock; Targeted Mutation; Mating System Heterozygote x Heterozygote (Female x Male) Species laboratory mouse Generation N5?PN2F1 (01-OCT-08) Donating Investigator Thomas Sudhof, Stanford University School of Medicine Description
Homozyogotes are viable, fertile and have normal body weight. This strain was created by excision of a neomycin cassette from the targeted mutation line (see Stock No. 006388 ) and wildtype levels of expression are restored for all splice variants. These mice have no obvious neuronal phenotype.Development
A targeting vector containing point mutations D303A, D357A, and D359A in exon 13 as well as a floxed neomycin gene in intron 12 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric animals were crossed with C57BL/6 and the neomycin gene was removed by crossing with a Cre deleter strain. The foundation line has been backcrossed at least five times to C57BL/6 by the donating laboratory.NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is on a mixed genetic background (4 out of 27 markers are segregating for non-C57BL/6 alleles). July, 2008.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Syt7
004950 B6.129S1-Syt7tm1Nan/J 006388 B6;129-Syt7tm1Sud/J View Strains carrying other alleles of Syt7 (2 strains)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
Syt7tm2Sud/Syt7tm2Sud
B6.129-Syt7tm1Sud
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:118319)
- mice are viable and fertile and have no neuronal phenotype
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Syt7tm2Sud | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Thomas C Sudhof | ||
| Mutation Made By | Thomas Sudhof, Stanford University School of Medicine | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Syt7, synaptotagmin VII | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | AI851541; B230112P13Rik; IPCA-7; MGC150517; PCANAP7; RIKEN cDNA B230112P13 gene; SYT-VII; expressed sequence AI851541; | ||
| Molecular Note | A targeting vector containing point mutations D303A, D357A, and D359A in exon 13 as well as a floxed neomycin gene in intron 12 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. The neomycin gene was removed by crossing with a Cre deleter strain. [MGI Ref ID J:118319] [MGI Ref ID J:131423] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Syt7tm1Sud, tm2Sud, , vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Maximov A; Lao Y; Li H; Chen X; Rizo J; Sorensen JB; Sudhof TC. 2008. Genetic analysis of synaptotagmin-7 function in synaptic vesicle exocytosis. Proc Natl Acad Sci U S A :. [PubMed: 18308933] [MGI Ref ID J:131423]
Additional References
Syt7tm2Sud related
Schonn JS; Maximov A; Lao Y; Sudhof TC; Sorensen JB. 2008. Synaptotagmin-1 and -7 are functionally overlapping Ca2+ sensors for exocytosis in adrenal chromaffin cells. Proc Natl Acad Sci U S A :. [PubMed: 18308932] [MGI Ref ID J:131424]Sudhof TC. 2007. A modified knock-in mutation of Syt7 MGI Direct Data Submission :. [MGI Ref ID J:118319]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $236.40 Female or Male Heterozygous for Syt7tm2Sud $291.90 Female or Male Homozygous for Syt7tm2Sud *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $472.80 Heterozygous for Syt7tm2Sud x Heterozygous for Syt7tm2Sud
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $307.40 Female or Male Heterozygous for Syt7tm2Sud $379.50 Female or Male Homozygous for Syt7tm2Sud *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $614.70 Heterozygous for Syt7tm2Sud x Heterozygous for Syt7tm2Sud
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Tel: 800.422.6423 or 207.288.5845
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