Strain Name: |
B6;129-Sncatm1Sud Sncbtm1.1Sud/J |
|---|---|
Stock Number: |
006390 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Snca; Sncatm1Sud; Sncb; Sncbtm1.1Sud; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Thomas Sudhof, Stanford University School of Medicine Generation N?+N1F3 (28-DEC-07) Strain Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. No protein product from these targeted genes is detected in brain tissue. Overall brain morphology and structure appears normal. No significant changes in the ultrastructure, short- or long-term synaptic plasticity, or in the pool size or replenishment of recycling synaptic vesicles is detected. Dopamine levels in the double targeted mice are decreased by approximately 20%, but dopamine uptake and release from isolated nerve terminals is normal. Serotonin levels are unchanged. This mutant mouse strain represents a model that may be useful in studies of synaptic function and neurodegenerative disease.Strain Development
A targeting vector introducing loxP sites on either side of exon 2 (encoding residues 1-41) was used to target the Sncb gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl +-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting mice were crossed with a protamine-Cre strain to remove the floxed exon. A targeting vector containing a neomycin resistance gene was used to delete the first coding exon (residues 1-40) of the Snca gene. The construct was electroporated into 129P2/OlaHsd –derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were crossed with C57BL/6 mice. The strain has been backcrossed to C57BL/6 for several generations by the donating laboratory. Mice carrying the independent targeted mutation genes were intercrossed to generate animals with both mutations.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Sncatm1Sud | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Thomas C Sudhof | ||
| Common Name(s) | alpha-; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14.1 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Snca, synuclein, alpha | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | MGC105443; MGC110988; NACP; PARK1; PARK4; PD1; alpha-synuclein; alphaSYN; | ||
| Molecular Note | The first coding exon, encoding residues 1-40, was deleted via homologous recombination. Immunoblotting confirmed the lack of protein in mutant mice brains. [MGI Ref ID J:93686] | ||
| Allele Symbol | Sncbtm1.1Sud | ||
| Allele Name | targeted mutation 1.1, Thomas C Sudhof | ||
| Common Name(s) | BSR; beta-; | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Sncb, synuclein, beta | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | AI838531; betaSYN; expressed sequence AI838531; | ||
| Molecular Note | Sncbtm1Sud mice were crossed with mice expressing cre in the germline, thereby removing floxed exon 2, which encodes amino acids 1-41. [MGI Ref ID J:93544] | ||
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Sncatm1Sud
Sncbtm1.1Sud
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, homozygotes or heterozygotes are intercrossed. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Snca
003692 B6;129X1-Sncatm1Rosl/J 008132 STOCK Tg(THY1-Snca)M1mSud/J View Strains carrying other alleles of Snca (2 strains)
008133 B6.129-Sncbtm1Sud/J
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
Snca relatedNeurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
Parkinson's Disease (synuclein mutants)
Neurobiology Research
Parkinson's Disease
References
Selected Reference(s)
Additional ReferencesChandra S; Fornai F; Kwon HB; Yazdani U; Atasoy D; Liu X; Hammer RE; Battaglia G; German DC; Castillo PE; Sudhof TC. 2004. Double-knockout mice for alpha- and beta-synucleins: effect on synaptic functions. Proc Natl Acad Sci U S A 101(41):14966-71. [PubMed: 15465911] [MGI Ref ID J:93544]
Price and Supply Information
| Strain Name: | B6;129-Sncatm1Sud Sncbtm1.1Sud/J |
| Stock Number: | 006390 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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