Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+N1F7 (16-APR-09)   Donating Investigator Thomas Sudhof,   Stanford University School of Medicine

Description
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. No protein product from these targeted genes is detected in brain tissue. Overall brain morphology and structure appears normal. No significant changes in the ultrastructure, short- or long-term synaptic plasticity, or in the pool size or replenishment of recycling synaptic vesicles is detected. Dopamine levels in the double targeted mice are decreased by approximately 20%, but dopamine uptake and release from isolated nerve terminals is normal. Serotonin levels are unchanged. This mutant mouse strain represents a model that may be useful in studies of synaptic function and neurodegenerative disease.

Development
A targeting vector introducing loxP sites on either side of exon 2 (encoding residues 1-41) was used to target the Sncb gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl ⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting mice were crossed with a protamine-Cre strain to remove the floxed exon. A targeting vector containing a neomycin resistance gene was used to delete the first coding exon (residues 1-40) of the Snca gene. The construct was electroporated into 129P2/OlaHsd ?derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were crossed with C57BL/6 mice. The strain has been backcrossed to C57BL/6 for several generations by the donating laboratory. Mice carrying the independent targeted mutation genes were intercrossed to generate animals with both mutations.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Snca

003692	B6;129X1-Sncatm1Rosl/J
008132	STOCK Tg(THY1-Snca)M1mSud/J

View Strains carrying other alleles of Snca     (2 strains)

Strains carrying other alleles of Sncb

008133

B6.129-Sncbtm1Sud/J

View Strains carrying other alleles of Sncb     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Snca^tm1Sud/Snca^tm1Sud Sncb^tm1.1Sud/Sncb^tm1.1Sud

        involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ

• nervous system phenotype
• abnormal striatum morphology (MGI Ref ID J:93544)
  • dopamine levels decreased by 18% in the striatum but metabolic levels are normal
• decreased dopamine level (MGI Ref ID J:93544)
  • dopamine levels decreased by 18% in the striatum but metabolic levels are normal

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
Parkinson's Disease
      synuclein mutants

Snca related

Neurobiology Research
Parkinson's Disease

Genes & Alleles

Gene & Allele Information

Allele Symbol		Sncatm1Sud
Allele Name		targeted mutation 1, Thomas C Sudhof
Allele Type		Targeted (knock-out)
Common Name(s)		alpha-;
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14.1
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Snca, synuclein, alpha
Chromosome		6
Gene Common Name(s)		MGC105443; MGC110988; NACP; PARK1; PARK4; PD1; alpha-synuclein; alphaSYN;
Molecular Note		The first coding exon, encoding residues 1-40, was deleted via homologous recombination. Immunoblotting confirmed the lack of protein in mutant mice brains. [MGI Ref ID J:93686]
Allele Symbol		Sncbtm1.1Sud
Allele Name		targeted mutation 1.1, Thomas C Sudhof
Allele Type		Targeted (knock-out)
Common Name(s)		BSR; beta-;
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Sncb, synuclein, beta
Chromosome		13
Gene Common Name(s)		AI838531; betaSYN; expressed sequence AI838531;
Molecular Note		Sncbtm1Sud mice were crossed with mice expressing cre in the germline, thereby removing floxed exon 2, which encodes amino acids 1-41. [MGI Ref ID J:93544]

Genotyping

Genotyping Information

Genotyping Protocols

Snca^tm1Sud, Standard PCR
Sncb^tm1.1Sud, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Chandra S; Fornai F; Kwon HB; Yazdani U; Atasoy D; Liu X; Hammer RE; Battaglia G; German DC; Castillo PE; Sudhof TC. 2004. Double-knockout mice for alpha- and beta-synucleins: effect on synaptic functions. Proc Natl Acad Sci U S A 101(41):14966-71. [PubMed: 15465911]  [MGI Ref ID J:93544]

Additional References

Snca^tm1Sud related

Chandra S; Gallardo G; Fernandez-Chacon R; Schluter OM; Sudhof TC. 2005. Alpha-synuclein cooperates with CSPalpha in preventing neurodegeneration. Cell 123(3):383-96. [PubMed: 16269331]  [MGI Ref ID J:115193]

Schluter OM; Fornai F; Alessandri MG; Takamori S; Geppert M; Jahn R; Sudhof TC. 2003. Role of alpha-synuclein in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in mice. Neuroscience 118(4):985-1002. [PubMed: 12732244]  [MGI Ref ID J:93686]

Sncb^tm1.1Sud related

Chandra S; Gallardo G; Fernandez-Chacon R; Schluter OM; Sudhof TC. 2005. Alpha-synuclein cooperates with CSPalpha in preventing neurodegeneration. Cell 123(3):383-96. [PubMed: 16269331]  [MGI Ref ID J:115193]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygotes or heterozygotes are intercrossed.
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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