Strain Name: |
FVB-Tg(Ckmm-cre)5Khn/J |
|---|---|
Stock Number: |
006405 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Ckm; cre; Tg(Ckmm-cre)5Khn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator C. Ronald Kahn, Joslin Diabetes Center Generation ?+F1 (03-DEC-07) Strain Description
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice have the Cre recombinase gene driven by the muscle creatine kinase (MCK or Ckm) promoter. Cre activity is observed in skeletal and cardiac muscle. When bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in skeletal and cardiac muscle deletion of the flanked genome.Strain Development
A transgene was designed with a cre recombinase cDNA sequence (with a SV-40 large T antigen nuclear localization signal and poly(A) signal) inserted in place of the translation initiation site of the Ckm gene. This construct was injected into fertilized FVB embryos which were then implanted into CD1 foster mothers. Chimeric mice were bred to FVB inbred animals. The resulting offspring (founder line 5) were bred together for many generations prior to arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Ckmm-cre)5Khn | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, C Ronald Kahn | ||
| Common Name(s) | Ckmm-NLS-cre; MCK-cre; MCK-cre5; MCKCre+; mckCRE; | ||
| Mutation Made By | C. Ronald Kahn, Joslin Diabetes Center | ||
| Strain of Origin | FVB | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ckm, creatine kinase, muscle, mouse, laboratory | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a muscle creatine kinase promoter, which directs expression in the heart and skeletal muscle. [MGI Ref ID J:51266] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ckmm-cre)5Khn
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. While the donating investigator has not attempted to make this strain homozygous, viability of homozygous mice is expected. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Tg(Ckmm-cre)5Khn allele
006475 B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J View Strains carrying Tg(Ckmm-cre)5Khn (1 strain)
006781 C57BL/6-Tg(Ckm-DGAT2)10Far/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cardiovascular Research (Cre-lox System)
Cre-lox System (Cre Recombinase Expression)
Diabetes and Obesity Research
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesBruning JC; Michael MD; Winnay JN; Hayashi T; Horsch D; Accili D; Goodyear LJ; Kahn CR. 1998. A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance. Mol Cell 2(5):559-69. [PubMed: 9844629] [MGI Ref ID J:51266]
Price and Supply Information
| Strain Name: | FVB-Tg(Ckmm-cre)5Khn/J |
| Stock Number: | 006405 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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