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| ChAT-IRES-Cre knockin mice have an "IRES-Cre" sequence inserted downstream of the choline acetyltransferase stop codon such that cre expression is controlled by the endogenous promoter. Chat gene expression, however, is unaffected. Cre recombinase activity is reported in all cholinergic neurons. These mice may be useful for "Cre-lox" technology applications in neurobiology, including studies of motor function, learning and memory, Alzheimer's disease, and Down syndrome, and in obesity and diabetes research. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6;129S6-Chattm1(cre)Lowl/J (Changed: 24-APR-13 ) Type Mutant Stock; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 30-APR-08 Species laboratory mouse Generation N?+F10 (18-JUN-12)
Generation DefinitionsDonating Investigator Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard) Description
Mice that are homozygous for the ChAT-IRES-Cre knockin are viable and fertile. An "IRES-Cre" sequence is inserted downstream of the stop codon such that cre expression is controlled by the endogenous Chat gene promoter. Chat gene expression, however, is unaffected. Cre recombinase activity is reported in all cholinergic neurons. These mice may be useful for "Cre-lox" technology applications in neurobiology, including studies of motor function, learning and memory, Alzheimer's disease, and Down syndrome, and in obesity and diabetes research.View cre expression characterization.
Development
A targeting vector was designed to insert an optimized internal ribosome entry site-linked Cre recombinase gene (followed by an frt-flanked neomycin resistance cassette) downstream of the stop codon of the endogenous gene. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to C57BL/6 mice. The resulting mutant offspring were bred to wildtype siblings, and maintained as such prior to arrival at The Jackson Laboratory Repository in 2006. These mice still retain the frt-flanked neomycin resistance cassette.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
016920 B6.129-Chattm1Jrs/J 014545 B6.Cg-Tg(Chat-COP4*H134R/EYFP)5Gfng/J 014546 B6.Cg-Tg(Chat-COP4*H134R/EYFP)6Gfng/J 007902 B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J 008364 B6;129-Chattm1(cre/ERT)Nat/J
Additional Web Information
Introduction to Cre-lox technology
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Myasthenic Syndrome, Congenital, Associated with Episodic Apnea (CHAT)
assigned by genotype
Chattm1(cre)Lowl/Chat+
involves: 129S6/SvEvTac * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- heterozgyotes are viable and fertile; cre recombinase activity is detected in all cholinergic neurons heterozygotes are viable and fertile; cre recombinase activity is detected in all cholinergic neurons (MGI Ref ID J:114556)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Alzheimer's Disease
Cre-lox System
Cre Recombinase expression in neural tissue
Down syndrome
Research Tools
Cre-lox System
Cre Recombinase Expression
Diabetes and Obesity Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Chattm1(cre)Lowl | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Bradford B Lowell | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ChAT-IRES-Cre; | ||
| Mutation Made By | Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard) | ||
| Strain of Origin | 129S6/SvEvTac | ||
| ES Cell Line Name | W4 | ||
| ES Cell Line Strain | 129S6/SvEvTac | ||
| Site of Expression | Cre recombinase activity is reported in all cholinergic neurons. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Chat | ||
| Molecular Note | An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. The neo cassette was excised with Flp recombinase. Cre recombinase activity is reported in all cholinergic neurons. This allele generated from Chattm2(cre)Lowl. [MGI Ref ID J:114556] [MGI Ref ID J:169562] | ||
| Gene Symbol and Name | Chat, choline acetyltransferase | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | B230380D24Rik; CHOACTASE; CMS1A; CMS1A2; RIKEN cDNA B230380D24 gene; | ||
| Allele Symbol | Chattm2(cre)Lowl | ||
| Allele Name | targeted mutation 2, Bradford B Lowell | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ChAT-IRES-Cre; | ||
| Strain of Origin | 129S6/SvEvTac | ||
| Gene Symbol and Name | Chat, choline acetyltransferase | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | B230380D24Rik; CHOACTASE; CMS1A; CMS1A2; RIKEN cDNA B230380D24 gene; | ||
| Driver Note | Chat | ||
| Molecular Note | An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. This allele was used to generate Chattm1(cre)Lowl. [MGI Ref ID J:169562] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Chattm1(cre)Lowl,Separated MCA
Chattm1(cre)Lowl, Separated PCR
Generic Cre Quantitative PCR, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Lowell BB; Olson D; Yu J. 2006. Development and phenotype of ChAT-IRES-Cre mice MGI Direct Data Submission :. [MGI Ref ID J:114556]
Additional References
Chattm1(cre)Lowl relatedChattm2(cre)Lowl relatedDaigle TL; Caron MG. 2012. Elimination of GRK2 from Cholinergic Neurons Reduces Behavioral Sensitivity to Muscarinic Receptor Activation. J Neurosci 32(33):11461-6. [PubMed: 22895728] [MGI Ref ID J:187717]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 MGI Direct Data Submission :. [MGI Ref ID J:184579]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Ivanova E; Hwang GS; Pan ZH. 2010. Characterization of transgenic mouse lines expressing Cre recombinase in the retina. Neuroscience 165(1):233-43. [PubMed: 19837136] [MGI Ref ID J:158209]
Katayama K; Leslie JR; Lang RA; Zheng Y; Yoshida Y. 2012. Left-Right Locomotor Circuitry Depends on RhoA-Driven Organization of the Neuroepithelium in the Developing Spinal Cord. J Neurosci 32(30):10396-407. [PubMed: 22836272] [MGI Ref ID J:186538]
Lefebvre JL; Kostadinov D; Chen WV; Maniatis T; Sanes JR. 2012. Protocadherins mediate dendritic self-avoidance in the mammalian nervous system. Nature 488(7412):517-21. [PubMed: 22842903] [MGI Ref ID J:186772]
Madisen L; Mao T; Koch H; Zhuo JM; Berenyi A; Fujisawa S; Hsu YW; Garcia AJ 3rd; Gu X; Zanella S; Kidney J; Gu H; Mao Y; Hooks BM; Boyden ES; Buzsaki G; Ramirez JM; Jones AR; Svoboda K; Han X; Turner EE; Zeng H. 2012. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nat Neurosci 15(5):793-802. [PubMed: 22446880] [MGI Ref ID J:182204]
Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40. [PubMed: 20023653] [MGI Ref ID J:155793]
Magno L; Kretz O; Bert B; Ersozlu S; Vogt J; Fink H; Kimura S; Vogt A; Monyer H; Nitsch R; Naumann T. 2011. The integrity of cholinergic basal forebrain neurons depends on expression of Nkx2-1. Eur J Neurosci 34(11):1767-82. [PubMed: 22098391] [MGI Ref ID J:184343]
Rossi J; Balthasar N; Olson D; Scott M; Berglund E; Lee CE; Choi MJ; Lauzon D; Lowell BB; Elmquist JK. 2011. Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab 13(2):195-204. [PubMed: 21284986] [MGI Ref ID J:169562]
Siegert S; Cabuy E; Scherf BG; Kohler H; Panda S; Le YZ; Fehling HJ; Gaidatzis D; Stadler MB; Roska B. 2012. Transcriptional code and disease map for adult retinal cell types. Nat Neurosci 15(3):487-95, S1-2. [PubMed: 22267162] [MGI Ref ID J:182307]
Sohn JW; Harris LE; Berglund ED; Liu T; Vong L; Lowell BB; Balthasar N; Williams KW; Elmquist JK. 2013. Melanocortin 4 receptors reciprocally regulate sympathetic and parasympathetic preganglionic neurons. Cell 152(3):612-9. [PubMed: 23374353] [MGI Ref ID J:193452]
Zariwala HA; Borghuis BG; Hoogland TM; Madisen L; Tian L; De Zeeuw CI; Zeng H; Looger LL; Svoboda K; Chen TW. 2012. A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. J Neurosci 32(9):3131-41. [PubMed: 22378886] [MGI Ref ID J:182205]
Martinez TL; Kong L; Wang X; Osborne MA; Crowder ME; Van Meerbeke JP; Xu X; Davis C; Wooley J; Goldhamer DJ; Lutz CM; Rich MM; Sumner CJ. 2012. Survival Motor Neuron Protein in Motor Neurons Determines Synaptic Integrity in Spinal Muscular Atrophy. J Neurosci 32(25):8703-8715. [PubMed: 22723710] [MGI Ref ID J:185657]
Pecho-Vrieseling E; Sigrist M; Yoshida Y; Jessell TM; Arber S. 2009. Specificity of sensory-motor connections encoded by Sema3e-Plxnd1 recognition. Nature 459(7248):842-6. [PubMed: 19421194] [MGI Ref ID J:149553]
Rossi J; Balthasar N; Olson D; Scott M; Berglund E; Lee CE; Choi MJ; Lauzon D; Lowell BB; Elmquist JK. 2011. Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab 13(2):195-204. [PubMed: 21284986] [MGI Ref ID J:169562]
Wang Z; Wang B; Yang L; Guo Q; Aithmitti N; Songyang Z; Zheng H. 2009. Presynaptic and postsynaptic interaction of the amyloid precursor protein promotes peripheral and central synaptogenesis. J Neurosci 29(35):10788-801. [PubMed: 19726636] [MGI Ref ID J:152457]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony at The Jackson Laboratory Repository, homozygous mice are bred together. Mating System Homozygote x Homozygote (Female x Male) 30-APR-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Chattm1(cre)Lowl
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Chattm1(cre)Lowl x Homozygous for Chattm1(cre)Lowl Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Chattm1(cre)Lowl
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Chattm1(cre)Lowl x Homozygous for Chattm1(cre)Lowl Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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